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981.
H. Jonsson K. Viken Sandnes D. Schiedek R. Schneider B. E. Grsvik A. Goksyr 《Marine environmental research》2004,58(2-5):655
In an attempt to learn more about the cytochrome P450 (CYP) system of mussels, we used protein databases and alignment software to extract highly conserved CYP sequences. From these alignments synthetic peptides were produced and used for rabbit immunisation, which yielded polyclonal antibodies against the CYP families 2 and 4. The antibodies were evaluated with Western Blot and ELISA assays, using digestive gland microsomal samples from the mussel Mytilus edulis. Western Blots revealed immunoreactions for both antibodies. The anti-CYP2 sequence rendered one major immunopositive protein of ≈49 kDa size, and weak signals for proteins of ≈41 and 56 kDa size. The anti-CYP4 sequence rendered two major bands of ≈56 and 59 kDa size, and also a weak immunoreaction with a protein of ≈43 kDa size. ELISA rendered only weak signals even with a 1:50 dilution of IgG-purified serum. A 10-day exposure to Aroclor 1254 did not appear to affect any of the immunopositive proteins, while total PCBs in soft bodies increased from 14–40 ng/g DW in controls to 373–638 ng/g DW in exposed mussels. 相似文献
982.
E. García-Luque T. A. DelValls C. Casado-Martínez J. M. Forja A. Gmez-Parra 《Marine environmental research》2004,58(2-5):671
We describe the effect of heavy metals Zn, Cd, Pb and Cu on the induction of methallothioneins on the clam Scrobicularia plana along a salinity gradient simulated under laboratory conditions. The clams were exposed to constant heavy metal concentrations in a dynamic estuary simulator during a 15-day assay to investigate possible induction of metal-binding proteins in them. The concentration of heavy metals in water was analysed. Clams were analysed for methallothionein concentrations. The speciation of Zn, Cd, Pb and Cu along the salinity gradient was modelled. Zn showed the highest concentrations and its prevalent species was the free ion. Intersite differences have been observed in methallothionein concentration and related to the salinity gradient. It seems that synthesis of methallothioneins is the result of physiological forces acting in concert with the changes in the chemical speciation of metals, owing to the trace metals uptake is controlled by means of an interaction of physiology and physicochemistry. 相似文献
983.
From the experimental studies in recent years, it has become known that when a wave breaks directly on a vertical faced coastal structure, high magnitude impact pressures are produced. The theoretical and experimental studies show that the dynamic response of such structures under wave impact loading is closely dependent on the magnitude and duration of the load history. The dynamic analysis and design of a coastal structure can be succeeded provided the design load history for the wave impact is available. Since these types of data are very scarce, it is much more convenient to follow a method which is based on static analysis for the dynamic design procedure. Therefore, to facilitate the dynamic design of a vertical plate that is exposed to breaking wave impact, a multiplication factor called “dynamic magnification factor” is herein presented which is defined as the ratio of the maximum value of the dynamic response to that found by static analysis. The computational results of the present study show that the dynamic magnification factor is a useful ratio to transfer the results of static analysis to the dynamic design of a coastal plate for the maximum impact pressure conditions of pmax/γH0≤18. 相似文献
984.
This article describes absolute calibration results for both JASON-1 and TOPEX Side B (TSB) altimeters obtained at the Lake Erie calibration site, Marblehead, Ohio, USA. Using 15 overflights, the estimated JASON altimeter bias at Marblehead is 58 ± 38 mm, with an uncertainty of 19 mm based on detailed error analysis. Assuming that the TSB bias is negligible, relative bias estimates using both data from the TSB-JASON formation flight period and data from 48 water level gauges around the entire Great Lakes confirmed the Marblehead results. Global analyses using both the formation flight data and dual-satellite (TSB and JASON) crossovers yield a similar relative bias estimate of 146 ± 59 mm, which agrees well with open ocean absolute calibration results obtained at Harvest, Corsica, and Bass Strait (e.g., Watson et al. 2003). We find that there is a strong dependence of bias estimates on the choice of sea state bias (SSB) models. Results indicate that the invariant JASON instrument bias estimated oceanwide is 71 mm, with additional biases of 76 mm or 28 mm contributed by the choice of Collecte Localisation Satellites (CLS) SSB or Center for Space Research (CSR) SSB model, respectively. Similar analysis in the Great Lakes yields the invariant JASON instrument bias at 19 mm, with the SSB contributed biases at 58 mm or 13 mm, respectively. The reason for the discrepancy is currently unknown and warrants further investigation. Finally, comparison of the TOPEX/POSEIDON mission (1992-2002) data with the Great Lakes water level gauge measurements yields a negligible TOPEX altimeter drift of 0.1 mm/yr. 相似文献
985.
A statistical model is developed to predict wave overtopping volume and rate of extreme waves on a fixed deck. The probability density function for the volume and rate of overtopping water are formulated based on the truncated Weibull distribution with the assumption of local sinusoidal profile for small amplitude waves. Sensitivity to the wave nonlinearity parameter and deck clearance is discussed. The statistical model is compared to laboratory data of the instantaneous free surface elevation measured in front of a fixed deck, and overtopping volume and overtopping rate measured at the leading edge of the deck. The statistical theory compared well with the measured exceedance probability seaward of the deck. The model prediction of the exceedance probability of deck overtopping gave qualitatively good agreement for large overtopping values. 相似文献
986.
Available measurements of foundation behaviour of the 13 concrete gravity platforms installed in the North Sea in the years 1973–1978 are presented. The measurements cover both the installation phase and the early part of the operation period. For the installation phase, measurements of dowel and skirt penetration resistance, base contact stresses, tilt, piping and erosion, are presented. For the operation period, the presentation includes measurements of settlements, pore water pressures, base contact stresses, permanent lateral displacements, cyclic displacements, dynamic behaviour and erosion. The measurements are used to back calculate soil properties and to evaluate the adequacy of the design procedures which have been used. The observations give valuable experience with respect to the foundation performance of offshore gravity platforms. 相似文献
987.
Tiago M. Alves Vasilios Lykousis Dimitris Sakellariou Stamatina Alexandri Paraskevi Nomikou 《Geo-Marine Letters》2007,27(1):41-61
Bathymetric, 9.5-kHz long-range sidescan sonar (OKEAN), seismic reflection and sediment-core data are used in the analysis
of two tectonic troughs south of Crete, Eastern Mediterranean Sea. Here, up to 1.2 s two-way travel time (TWTT) of strata
have accumulated since the Middle Miocene in association with extension in the South Aegean region. The study area comprises
>100-km- long by >25-km-wide basins filled by sediments subdivided into two seismic units: (1) an upper Unit 1 deposited in
sub-basins which follow the present-day configuration of the southern Cretan margin; (2) a basal Unit 2, more than 500 ms
(TWTT) thick, accumulated in deeper half-graben/grabens distinct from the present-day depocentres. Both units overlap a locally
stratified Unit 3 comprising the pre-Neogene core complex of Crete and Gavdos. In this work, the interpreted seismic units
are correlated with the onshore stratigraphy, demonstrating that denudation processes occurring on Crete and Gavdos in response
to major tectonic events have been responsible for high sedimentation rates along the proximal southern Cretan margin. Consequently,
topographically confined sedimentary units have been deposited south of Crete in the last 12 Ma, including turbidites and
other mass-flow deposits fed by evolving transverse and axial channel systems. Surface processes controlling facies distribution
include the direct inflow of sediment from alluvial-fan systems and incising mountain rivers onto the Cretan slope, where
significant sediment instability processes occur at present. In this setting, seismic profiles reveal eight different types
of stratigraphic contacts on basin-margin highs, and basinal areas show evidence of halokinesis and/or fluid escape. The acquired
data also show that significant changes to the margin’s configuration occurred in association with the post-Alpine tectonic
and eustatic episodes affecting the Eastern Mediterranean. 相似文献
988.
By using the NCEP/NCAR reanalysis data sets for 1951–2001, we study the characteristics of Pacific cyclones. It is shown that the northeast-southwest
direction is predominant in the displacements of cyclones in the North Pacific. We study the variability of the field of surface
atmospheric pressure in different phases of the Pacific decadal oscillation characterizing the temperature anomalies on the
surface of the ocean in the region bounded by 20 and 60°N. It is shown that the decadal variations of the North Atlantic Oscillation
supported by the large-scale anomalies of the Pacific decadal oscillation is the most important cause of natural decadal oscillations
in the European region. We study and evaluate the regional response to the Pacific decadal oscillation by using, as an example,
the analysis of variations of the discharge of European rivers.
__________
Translated from Morskoi Gidrofizicheskii Zhurnal, No. 4, pp. 13–23, July–August, 2007. 相似文献
989.
990.
It has been known that the axisymmetric Cauchy–Poisson problem for dispersive water waves is well posed in the sense of stability. Thereby time evolution solutions of wave propagation depend continuously on initial conditions. However, in this paper, it is demonstrated that the axisymmetric Cauchy–Poisson problem is ill posed in the sense of stability for a certain class of initial conditions, so that the propagating solutions do not depend continuously on the initial conditions. In order to overcome the difficulty of the discontinuity, Landweber–Fridman's regularization, famous and well known in applied mathematics, are introduced and investigated to learn whether it is applicable to the present axisymmetric wave propagation problem. From the numerical experiments, it is shown that stable and accurate solutions are realized by the regularization, so that it can be applicable to the determination of the ill-posed Cauchy–Poisson problem. 相似文献