Oxygen consumption rate of shrimp exposed to crude oil extract

The shallow water shrimp Palaemon adspersus was exposed to 200, 100 and 0 ppb WSF of crude oil for 14 days after which the oxygen consumption rate was measured in a closed chamber with an electrode. Previous work has shown a changed oxygen consumption rate in animals exposed to WSF in concentrations 100 to 1000 times the concentrations used in the present experiments. The oxygen consumption rate was also significantly increased by the low concentrations used in the present work.Although the oxygen consumption rate was significantly increased, as an effect of WSF-pollution, the method is very time-consuming.     

Chloride retention in forest soil by microbial uptake and by natural chlorination of organic matter

Inorganic chlorine (i.e. chloride; Cl_in) is generally considered inert in soil and is often used as a tracer of soil and ground water movements. However, recent studies indicate that substantial retention or release of Cl_in can occur in soil, but the rates and processes responsible under different environmental conditions are largely unknown. We performed ³⁶Cl tracer experiments which indicated that short-term microbial uptake and release of Cl_in, in combination with more long-term natural formation of chlorinated organic matter (Cl_org), caused Cl_in imbalances in coniferous forest soil. Extensive microbial uptake and release of Cl_in occurred over short time scales, and were probably associated with changes in environmental conditions. Up to 24% of the initially available Cl_in within pore water was retained by microbial uptake within a week in our experiments, but most of this Cl_in was released to the pore water again within a month, probably associated with decreasing microbial populations. The natural formation of Cl_org resulted in a net immobilization of 4% of the initial pore water Cl_in over four months. If this rate is representative for the area where soil was collected, Cl_org formation would correspond to a conversion of 25% of the yearly wet deposition of Cl_in. The study illustrates the potential of two Cl_in retaining processes in addition to those previously addressed elsewhere (e.g. uptake of chloride by vegetation). Hence, several processes operating at different time scales and with different regulation mechanisms can cause Cl_in imbalances in soil. Altogether, the results of the present study (1) provide evidence that Cl_in cannot be assumed to be inert in soil, (2) show that microbial exchange can regulate pore water Cl_in concentrations and (3) confirm the controversial idea of substantial natural chlorination of soil organic matter.     

Phytoplankton response to climate changes in Lake Baikal during the Holocene and Kazantsevo Interglacials assessed from sedimentary pigments

Lake Baikal, an ancient pristine lake in Siberia, has accumulated sediment deposits that span 25 million years. These deposits have the potential to provide a long-term record of climate changes and their interaction with the ecology of the lake. In order to investigate whether sedimentary phytoplankton pigments could be used to reconstruct past changes in total phytoplankton abundance and productivity, we compared the spatial variability in sedimentary pigment distributions in Holocene cores from three separate regions of the lake; Vidrino in the south, Posolski on Selenga Delta and Continent Ridge in the north. Furthermore, we present the first continuous sedimentary pigment and organic carbon sequence of the Kazantsevo interglacial (roughly a time equivalent to the European Eemian, and Marine Isotopic Stage MIS5e) at a resolution of approximately 150 years. Results of the spatial study showed marked differences in the sediment pigment deposition. Lowest chlorophyll a plus its degradation products versus organic carbon ratios (Chlas/TOC) indicating lowest production, but highest variability with time (indicating strongest climatic oscillations) were found at Continent Ridge. The study of sedimentary pigments deposited during the last two interglacial periods at Continent Ridge showed Chlas/TOC ratios 50–1000 times higher during the Kazantsevo Interglacial compared to the glacial periods, whereas the TOC content was only five times higher, thus indicating the significance of the Chlas/TOC ratio for the reconstruction of the phytoplankton abundance and productivity. Strong oscillations occurred during the Kazantsevo Interglacial within centennial time scales. Chlorophyllb plus its degradation products provided additional information on the past development of Chlorophyceae. Highest Chlas/TOC ratios were found during the early Holocene at approximately 9 kyr BP. Indications of short phytoplankton production maxima were also found during the late Atlantic (6 kyr BP) and at the Subboreal/Subatlantic transition (3 kyr BP). From this we conclude that sedimentary chlorophyll a is a reliable indicator of phytoplanktonic response to climate changes and may serve for␣validation of future climate scenarios in continental regions.     

Alteration minerals,fluids, and gases on early Mars: Predictions from 1‐D flow geochemical modeling of mineral assemblages in meteorite ALH 84001

Clay minerals, although ubiquitous on the ancient terrains of Mars, have not been observed in Martian meteorite Allan Hills (ALH) 84001, which is an orthopyroxenite sample of the early Martian crust with a secondary carbonate assemblage. We used a low‐temperature (20 °C) one‐dimensional (1‐D) transport thermochemical model to investigate the possible aqueous alteration processes that produced the carbonate assemblage of ALH 84001 while avoiding the coprecipitation of clay minerals. We found that the carbonate in ALH 84001 could have been produced in a process, whereby a low‐temperature (~20 °C) fluid, initially equilibrated with the early Martian atmosphere, moved through surficial clay mineral and silica‐rich layers, percolated through the parent rock of the meteorite, and precipitated carbonates (thereby decreasing the partial pressure of CO₂) as it evaporated. This finding requires that before encountering the unweathered orthopyroxenite host of ALH 84001, the fluid permeated rock that became weathered during the process. We were able to predict the composition of the clay minerals formed during weathering, which included the dioctahedral smectite nontronite, kaolinite, and chlorite, all of which have been previously detected on Mars. We also calculated host rock replacement in local equilibrium conditions by the hydrated silicate talc, which is typically considered to be a higher temperature hydrothermal phase on Earth, but may have been a common constituent in the formation of Martian soils through pervasive aqueous alteration. Finally, goethite and magnetite were also found to precipitate in the secondary alteration assemblage, the latter associated with the generation of H₂. Apparently, despite the limited water–rock interaction that must have led to the formation of the carbonates ~ 3.9 Ga ago, in the vicinity of the ALH 84001 source rocks, clay formation would have been widespread.     

Variation in Lake Baikal's phytoplankton distribution and fluvial input assessed by SeaWiFS satellite data

The current behaviour of selected climate proxies in Lake Baikal was assessed by remote sensing analyses of ‘Sea viewing Wide Field of view Sensor’ (SeaWiFS) satellite data. Suitable proxies include optically visible water constituents such as phytoplankton, suspended terrigenous matter and yellow substance. These limnological parameters reflect the present-day climate bioproductivity and the river discharge in the catchment area.A biological and geochemical ground truth data set for Lake Baikal was established with the help of members of the paleoclimate project ‘high-resolution CONTINENTal paleoclimate record in Lake Baikal’ (CONTINENT). For processing the SeaWiFS satellite data, the atmospheric correction was adapted to the case of Lake Baikal. Chlorophyll as a proxy for phytoplankton was quantified using global NASA ocean colour algorithms OC2 and OC4. In cases of no optical interferences by terrigenous input, the calculated chlorophyll concentrations in clear pelagic waters were within ±30% accuracy with the CONTINENT cruise data during the summers of 2001 and 2002. Within this range of accuracy, the SeaWiFS time series will be able to show the seasonal variations of chlorophyll of specified bio-optical provinces of Lake Baikal and of CONTINENT sites. In this study, the suspended matter as a proxy for the terrigenous input was calculated according to an empirical algorithm using ground truth data in the time frame of flooding events in summer 2001. The approach chosen correlates the suspended matter concentration with the remotely sensed parameter of ‘attenuation coefficient’ to account for the organic-rich terrigenous input that originates from the swampy watersheds.Seasonal and spatial information that is provided by the analyses of the SeaWiFS satellite data will assist paleoclimate researchers to interpret the autochthonous and allochthonous influences at the CONTINENT coring sites.     

40Ar‐39Ar and cosmic‐ray exposure ages of nakhlites—Nakhla,Lafayette, Governador Valadares—and Chassigny

Abstract– We present ⁴⁰Ar‐³⁹Ar dating results of handpicked mineral separates and whole‐rock samples of Nakhla, Lafayette, and Chassigny. Our data on Nakhla and Lafayette and recently reported ages for some nakhlites and Chassigny ( Misawa et al. 2006 ; Park et al. 2009 ) point to formation ages of approximately 1.4 Ga rather than 1.3 Ga that is consistent with previous suggestions of close‐in‐time formation of nakhlites and Chassigny. In Lafayette mesostasis, we detected a secondary degassing event at approximately 1.1 Ga, which is not related to iddingsite formation. It may have been caused by a medium‐grade thermal event resetting the mesostasis age but not influencing the K‐Ar system of magmatic inclusions and the original igneous texture of this rock. Cosmic‐ray exposure ages for these meteorites and for Governador Valadares were calculated from bulk rock concentrations of cosmogenic nuclides ³He, ²¹Ne, and ³⁸Ar. Individual results are similar to literature data. The considerable scatter of T₃, T₂₁, and T₃₈ ages is due to systematic uncertainties related to bulk rock and target element chemistry, production rates, and shielding effects. This hampers efforts to better constrain the hypothesis of a single ejection event for all nakhlites and Chassigny from a confined Martian surface terrain ( Eugster 2003 ; Garrison and Bogard 2005 ). Cosmic‐ray exposure ages from stepwise release age spectra using ³⁸Ar and neutron induced ³⁷Ar from Ca in irradiated samples can eliminate errors induced by bulk chemistry on production rates, although not from shielding conditions.     

Reconstructing the historical changes in Daphnia mean size and planktivorous fish abundance in lakes from the size of Daphnia ephippia in the sediment

The zooplankton community structure in lakes is highly influenced by size-selective predation by fish, with small zooplankton species dominating at high predation pressure. Remains of cladocerans are preserved in the sediment and may be used to trace historical changes in fish predation. We determined how contemporary data on planktivorous fish were related to the size of Daphnia ephippia (dorsal length) in the surface sediment (0-1 cm) of 52 mainly shallow lakes with contrasting densities of fish and nutrients (TP: 0.002-0.60 mg P l^-1). Density of fish expressed as catch per unit effort, in terms of numbers in multiple mesh-sized gill nets (CPUE_n), decreased significantly with increasing mean size of ephippia. The relationship was improved by adding TP as an independent variable, now explaining 90% of the variation in CPUE_n on the full data set covering lakes in Denmark, Greenland and New Zealand, and 78% if only data on Danish lakes were used. CPUE by weight of planktivorous fish and mean weight of Daphnia in the pelagial during summer were also related to ephippial size. By including contemporary data on established relationships between the sizes of egg-bearing female Daphnia and ephippia, we inferred changes in the CPUE_n, mean size of ephippia-bearing Daphnia and summer mean body weight of Daphnia from ephippial size in four lakes during the past 1-2 centuries. In a hypertrophic lake subject to periodic fish kills, Daphnia mean body weight was high and CPUE_n was low compared with those in two eutrophic lakes, while CPUE_n was low and Daphnia body weight was high in the least eutrophic, clearwater lake. Estimated CPUE_n and Daphnia mean weight in the surface sediment of these four lakes corresponded well with contemporary data. Only small changes in ephippial size with time were observed in the clearwater lake and in one of the lakes that had suffered early eutrophication, while major changes occurred in the two other lakes that had been subjected to a major increase in nutrient input or fish kills. We conclude that Daphnia ephippia preserved in the surface sediments of lakes may be a useful and efficient method to quantify the present-day abundance of planktivorous fish and Daphnia mean size. The method is particularly valid in surveys aimed to give a general picture of the fish stock and the ecological state in a set of lakes in a region rather than a precise estimate for a single lake. Though some evidence is provided, more work is needed to evaluate whether the equations are valid for hind-casting in down-core palaeoecological studies.     

Molecular approach to determine contributions of the protist community to particle flux

The importance of key taxonomic groups of eukaryotic phytoplankton as contributors to downward particle flux was evaluated near the European time-series station (ESTOC) in the eastern subtropical North Atlantic in March 2005. For the first time, molecular (cloning and sequencing) techniques were used to compare plankton communities from various depths in the euphotic zone with clone libraries from surface-tethered particle traps. Analyses of 18S rDNA clone libraries revealed compositional differences between the phytoplankton assemblages in the photic zone and those retrieved from shallow particle traps below, suggesting that not all phytoplankton contribute equally to particle flux. Contrary to expectations, our study also showed that it is not diatoms, despite their high abundance in the water column, but rather small phytoplankton taxa that dominated sequences recovered from trap material. We provide here first observational evidence that large taxa with mineral tests may not necessarily contribute more to export production than do smaller taxa even if the former are abundant in the water column.     

Shopping behaviour in the Øresund region before and after the establishment of the fixed link between Denmark and Sweden

In July 2000 the 15.5 km long fixed link across the Øresund was opened, connecting the Danish capital Copenhagen and the Swedish city of Malmö. With the decrease of the barrier an enlarged flow of shoppers was expected with Swedish customers availing themselves of the range of opportunities in the Copenhagen area and Danish customers making use of the much longer shop opening hours in Sweden. However, experience from other European border areas shows that customers tend to do their ordinary shopping in their home countries and that there must be significant differences in prices or ranges of goods to overcome the barrier of the border. Does the typical situation result from the rural nature of many border areas, and will matters be quite different in a metropolitan area? The purpose of the present paper is to describe the level and character of cross-border shopping by the residents of the Øresund region before and after the opening of the fixed link. Responses from about 4,000 people form the basis of the conclusion that a little less than a year after the opening of the fixed link the number of shopping trips across the Øresund had increased, but there was still no heavy flow of customers doing ordinary shopping in the neighbouring countries. In order to assess the level of cross-border shopping across the Øresund the findings are compared to those from surveys across the Danish-German land border.