Antiangiogenic activity of low-temperature lysozyme from a marine bacterium <Emphasis Type="Italic">in vivo</Emphasis> and <Emphasis Type="Italic">in vitro</Emphasis>

We extracted marine low-temperature lysozyme (MLTL), a novel lysozyme, from a marine microorganism through fermentation. Our previous study suggested that a low molecular weight (16 kDa) may exert anti-tumor activity through antiangiogenesis. In this study, we extracted a high weight (39 kDa) and investigated its antiangiogenic activity in vivo and in vitro. Using zebrafish embryos as an in vivo study model, we found that treatment with MLTL significantly inhibited the growth of subintestinal vessels (SIVs) in a dose-dependent manner and that 400 μg/ml MLTL was sufficient to block the growth of SIVs. An in vitro study conducted using human umbilical vein endothelial cells (HUVECs) revealed that MLTL suppressed the proliferation, migration and tube formation of HUVECs in a dose-dependent manner. Interestingly, assays by flow cytometry and DNA electrophoresis indicated that MLTL was able to induce apoptosis of HUVECs. Moreover, further study demonstrated that the disruption of intracellular Ca²⁺ homeostasis may play an important role in MLTL induced apoptosis of HUVECs. Taken together, the results of this study demonstrate for the first time that MLTL inhibits angiogenesis through its pleiotropic effects on vascular endothelial cells and induces apoptosis through regulation of cellular Ca²⁺ levels. The results of this study also revealed a possible mechanism underlying the antiangiogenic effect of MLTL and suggested that MLTL may be a promising new antiangiogenic agent for use in cancer therapy.     

耿达短水准异常与汶川8.0级地震

结合龙门山断裂带其他跨断层资料,对耿达短水准在汶川8.0级地震前后的观测资料进行了重新分析,发现耿达短水准在汶川地震前后的异常为当地居民修建生活小区所致,不应视为地震前兆异常。     

对秦岭造山带几个重大问题的认识

详细讨论了秦岭一大别造山带的几个重大问题，对它的发展阶段进行了新的划分，对其形成发表了新的观点：认为秦岭造山带、华北板块、扬子板块是中生代以来，在抽拉－逆冲岩片构造体制作用下形成的。对秦岭一大别造山带的表层构造特征以及它和深层构造的关系、花岗岩的成因类型、断层与界面的区分及成矿作用等都提出了和以往不同的认识。     

埃尔塔宁小行星质量的下限估计

埃尔塔宁小行星是一颗２１５ 万年前在南太平洋撞击地球的小行星,它也是目前唯一的深海区与地球碰撞的小行星。根据埃尔塔宁号和Ｐｏｌａｒｓｔｅｒｎ 号海洋调查船在陨击海区勘查的小行星残骸分布密度,我们估计埃尔塔宁小行星质量为２ ．３ ×１０１３ｇ ,对应于它的直径为０ ．２３ 公里。这比Ｋｙｔｅ 等人（１９８８） 估计的质量小了１０ 倍,然而它更接近埃尔塔宁小行星撞击事件的实际情况,可以作为埃尔塔宁小行星质量下限和半径下限更合理的估计值。     

M型恒星周围系外行星统计研究

M型恒星(M dwarf)是主序星中质量较小的恒星,也是银河系中数量最多的恒星类型,在其周围形成的行星通常距离主星较近,宜居带也比F、G、K型恒星更靠近主星,更有利于发现系外宜居行星.研究表明, M型恒星周围平均存在2.5颗小质量行星,约为F、 G、 K型恒星的3.5倍,但M型恒星周围巨行星的出现率(occurrence rate)则比F、 G、K型小一个量级.基于M型恒星周围发现的401颗行星的参数开展了统计研究,发现质量越大的行星平均轨道半长径越大.类地行星约占行星总数的74%,且轨道半长径均小于1 au,其中28颗行星具有潜在宜居性.根据行星质量-半径关系,在质量等于4倍地球质量(M⊕)处存在一拐点,除少数几颗行星外,大部分小于该质量的行星可能都是由约65%的硅酸盐和约35%的铁组成,大于该质量的行星半径则随质量增加而迅速增大.约60%的M型恒星周围的行星位于多行星系统且轨道分布紧密,相邻行星轨道在3:2、5:3及2:1等平运动共振位置处存在峰值. M型恒星的多行星系统形成与演化等问题对现今的行星形成理论提出了新挑战.     

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泥蚶锰超氧化物歧化酶基因的研究

Special primers were designed based on the sequence information of superoxide dismutase genes reported in the Iiterature and were used for RT-PCR and rapid amplification of cDNA ends(RACE) to clone Tegillarca granosaMn-superoxide dismutase gene successfully.The length cDNA of Tegillarca granosaMn-superoxide dismutase gene was of 635bp by including a 3^'untranslated region(UTR)of 230bp and an open reading frame(ORF) of 4.5bp encoding a polypeptide of 135 amino acids. TheTegillarca granosaMn-superoxide dismutase gene shares 66%~73% homology with Mizuhopecteb yessoensis^'s,Danio rerio^'s,Xenopus Laevis^'s,Human^'s,Callinectes sapidus^'sSOD CDS, while the corresponding amino acids shares 68%-78% homology and 80%~89% comparability. These indicate the SOD gene is high evolutional conservation in animals.     

Optimizing support vector machine learning for semi-arid vegetation mapping by using clustering analysis

In remote sensing communities, support vector machine (SVM) learning has recently received increasing attention. SVM learning usually requires large memory and enormous amounts of computation time on large training sets. According to SVM algorithms, the SVM classification decision function is fully determined by support vectors, which compose a subset of the training sets. In this regard, a solution to optimize SVM learning is to efficiently reduce training sets. In this paper, a data reduction method based on agglomerative hierarchical clustering is proposed to obtain smaller training sets for SVM learning. Using a multiple angle remote sensing dataset of a semi-arid region, the effectiveness of the proposed method is evaluated by classification experiments with a series of reduced training sets. The experiments show that there is no loss of SVM accuracy when the original training set is reduced to 34% using the proposed approach. Maximum likelihood classification (MLC) also is applied on the reduced training sets. The results show that MLC can also maintain the classification accuracy. This implies that the most informative data instances can be retained by this approach.     

基坑土钉墙支护施工与监测——陕西省人民医院门急诊医技大楼

本文介绍了土钉墙在陕西省人民医院门急诊医技大楼基坑支护中的应用,简述了工程设计方法,总结了施工经验,并对基坑支护体系变形观测结果进行了分析     

遥感影像混合像元分解中的端元选择方法综述

端元选择是进行遥感影像混合像元分解的首要步骤,也是最关键的步骤,其直接影响混合像元分解的精度。该文对遥感影像混合像元分解中端元产生的特定背景、当前存在的端元选择途径和端元选择方法进行综述,并通过对当前端元选择方法的分析,提出了选择或构造端元选择方法应遵循的几个原则。