Heterogeneous distribution of paramagnetic radicals in insoluble organic matter from the Orgueil and Murchison meteorites

An electron paramagnetic resonance (EPR) investigation was performed on the insoluble organic matter from the Orgueil and Murchison meteorites and on terrestrial coals with similar elemental composition. A complementary electron nuclear double resonance (ENDOR) study was also carried out. The measured g-factors of the observed paramagnetic radicals in the meteoritic organic matter exhibit a similar correlation with the chemical composition as for the type III (i.e., hydrogen-poor) terrestrial coals. The main result, obtained from EPR saturation and ¹H ENDOR enhancement measurements, showed that the effective local concentration in radicals of about 3 to 4 × 10¹⁹ spin.g⁻¹ in the meteoritic organic matter is much higher than the average concentration, hence the occurrence of radical-rich regions accounting for 5% and 20% of the total volume for Murchison and Orgueil, respectively. This heterogeneity of concentration seems to be unique among natural organic macromolecules. It is proposed that these radical-rich regions correspond to pristine parts of the organic matter synthesized in conditions close to those prevailing in the interstellar medium, and which have survived the hydrothermal process on the parent body.     

Age of settlement and accumulation rate of submarine “coralligène” (−10 to −60 m) of the northwestern Mediterranean Sea; relation to Holocene rise in sea level

Morphological and chronological studies have been carried out on coralline algal buildups (“coralligène”) situated between 10 and 60 m depth near Marseilles, and in Corsica (France). Despite the presence of occasionally sizeable quantities of iron hydroxide, these constructions prove to be a reliable material for radiocarbon dating. Ages obtained using this method range from 640±120 yrs B.P. (Corsica, Scandola Natural Reserve, −15 m) to 7760±80 yrs B.P. (Marseilles, Grand Congloue, −52 m). Internal erosion surfaces within the buildups give evidence of discontinuous development. The accumulation rate of the coralligène constructions is very low (0.006–0.83 mm yr⁻¹ according to the depth and time period). The higher values (0.53–0.83 mm yr⁻¹) were recorded for the deeper constructions. They correspond to a period between 8000 and 6000 yrs B.P. After 6000 yrs B.P., the only appreciable accumulation rates (0.11–0.42 mm yr⁻¹) were recorded for constructions situated between 10 and 35 m depth, whereas the accumulation rates of deep coralligène (> 50 m) appear to be low or zero. The age of the large constructions (overhang: 80 cm in width) is positively correlated with depth (r = 0.95; p < 0.005). Their development occurred during the Flandrian transgression. The oldest structures, today situated at 50 to 60 m depth, started to develop when water depth was probably no greater than 10 to 15 m. Apart from in strongly shaded fissures on rocky coasts and areas subjected to heavy sedimentation, the main framework building algal species was initially Mesophyllum lichenoides (Ellis) Lemoine, a high tolerant species to light, hydrodynamic energy and temperature. With the rise in sea level, the coralligène structure gradually became available to other less tolerant algal species (Lithophyllum, Lithothamnion), and the crustforming population diversified. Because of the good preservation of coralligène structures, the reliability of radiocarbon dating and the correlation between the age and bathymetric position of the large coralligène structures (except in areas of heavy sedimentation and fissures in shallow rocky coasts), these buildups are considered to be of use as biological indicators of variations in sea level.     

Potential of EPR imaging to detect traces of primitive life in sedimentary rocks

In the prospect of the search for traces of primitive life on Earth and Mars, we investigated the possibility to detect and visualize the spatial distribution of minute amounts of organic matter in ancient rocks, in a non-destructive way, by Electron Paramagnetic Resonance Imaging (EPRI). We studied a series of non- or moderately metamorphosed siliceous rocks (cherts) of different ages ranging from 45 Ma to 3490 Ma and embedding fossile organic matter. In the case of the oldest cherts containing only mature insoluble organic matter (IOM), with IOM^• radicals characterized by a single Electron Paramagnetic Resonance (EPR) line, we could obtain three-dimensional images with sub-millimetric resolution of the organic matter distribution inside samples containing as low as 10¹⁴–10¹⁵ radicals per gram. In the case of younger cherts, containing less mature organic matter, and thus several types of organic radicals, we showed that selective imaging of each type of radical was possible provided that the EPR spectra did not overlap. Selective imaging of either the organic radicals or of the oxygen vacancy (E' centres) of the mineral matrix, which are ubiquitous in siliceous rocks, was possible, selecting either one or the other paramagnetic species with high power in-phase, 1st harmonic detection or with 90°-out-of-phase, 2nd harmonic detection of the EPR. The influence of ferromagnetic inclusions in the mineral matrix on the EPRI of the organic matter was also addressed. Image artifacts due to the ferromagnetic resonance signal of these inclusions could be easily removed by background substraction from the EPR spectra before image reconstruction. We thus showed that selective imaging by EPR of minute amounts of fossile organic matter in ancient rocks could be possible despite the magnetic complexity of such materials.     

Tube wave to shear wave conversion at borehole plugs

In hydraulic fracturing experiments, perforation shots excite body and tube waves that sample, and thus can be used to characterize, the surrounding medium. While these waves are routinely employed in borehole operations, their resolving power is limited by the experiment geometry, the signal‐to‐noise ratio, and their frequency content. It is therefore useful to look for additional, complementary signals that could increase this resolving power. Tube‐to‐body‐wave conversions (scattering of tube to compressional or shear waves at borehole discontinuities) are one such signal. These waves are not frequently considered in hydraulic fracture settings, yet they possess geometrical and spectral attributes that greatly complement the resolution afforded by body and tube waves alone. Here, we analyze data from the Jonah gas field (Wyoming, USA) to demonstrate that tube‐to‐shear‐wave conversions can be clearly observed in the context of hydraulic fracturing experiments. These waves are identified primarily on the vertical and radial components of geophones installed in monitoring wells surrounding a treatment well. They exhibit a significantly lower frequency content (10–100 Hz) than the primary compressional waves (100–1000 Hz). Tapping into such lower frequencies could help to better constrain velocity in the formation, thus allowing better estimates of fracture density, porosity and permeability. Moreover, the signals of tube‐to‐shear‐wave conversion observed in this particular study provide independent estimates of the shear wave velocity in the formation and of the tube wave velocity in the treatment well.     

Rapid flow cytometric method for the assessment of toxic dinoflagellate cyst viability

The inadvertent transfer and dispersal of non-indigenous marine species via shipping ballast water is of increasing environmental concern. Despite a major global effort to develop new ballast water treatment technologies, their acceptance has been hampered by the lack of suitable indicator species for assessing treatment effectiveness. Resistant dinoflagellate cysts are one proposed test organism, however their use has been limited due to difficulties in assessing their viability after treatment. The paper describes the development of a rapid method to determine the viability of cysts of the dinoflagellate Alexandrium catenella using staining with SYTOX Green and flow-cytometric analysis. The viability of A. catenella cysts was inversely proportional to their ability to take up the stain. There was excellent agreement between cysts measured as viable/non-viable using flow cytometry and cyst viability determined in standard long-term germination tests. Advantages of the flow-cytometric method include high test precision and rapid testing times of < 2 days, compared to > 4 weeks using existing germination methods.     

Field application of close‐range digital photogrammetry (CRDP) for grain‐scale fluvial morphology studies

In situ measurement of grain‐scale fluvial morphology is important for studies on grain roughness, sediment transport and the interactions between animals and the geomorphology, topics relevant to many river practitioners. Close‐range digital photogrammetry (CRDP) and terrestrial laser scanning (TLS) are the two most common techniques to obtain high‐resolution digital elevation models (DEMs) from fluvial surfaces. However, field application of topography remote sensing at the grain scale is presently hindered mainly by the tedious workflow challenges that one needs to overcome to obtain high‐accuracy elevation data. A recommended approach for CRDP to collect high‐resolution and high‐accuracy DEMs has been developed for gravel‐bed flume studies. The present paper investigates the deployment of the laboratory technique on three exposed gravel bars in a natural river environment. In contrast to other approaches, having the calibration carried out in the laboratory removes the need for independently surveyed ground‐control targets, and makes for an efficient and effective data collection in the field. Optimization of the gravel‐bed imagery helps DEM collection, without being impacted by variable lighting conditions. The benefit of a light‐weight three‐dimensional printed gravel‐bed model for DEM quality assessment is shown, and confirms the reliability of grain roughness data measured with CRDP. Imagery and DEM analysis evidences sedimentological contrasts between gravel bars within the reach. The analysis of the surface elevations shows the effect variable grain‐size and sediment sorting have on the surface roughness. By plotting the two‐dimensional structure functions and surface slopes and aspects we identify different grain arrangements and surface structures. The calculation of the inclination index allows determining the surface‐forming flow direction(s). We show that progress in topography remote sensing is important to extend our knowledge on fluvial morphology processes at the grain scale, and how a technique customized for use by fluvial geomorphologists in the field benefits this progress. Copyright © 2016 John Wiley & Sons, Ltd.     

Alternate bars in a sandy gravel bed river: generation,migration and interactions with superimposed dunes

A field study was carried out to investigate the development of alternate bars in a secondary channel of the Loire River (France) as a function of discharge variations. We combined frequent bathymetric surveys, scour chains and stratigraphical analysis of deposits with measurements and modelling of flow dynamics. The channel exhibited migrating bars, non‐migrating bars and superimposed dunes. Possible mechanisms of bar initiation were found to be chutes associated with changes of bank direction and instability resulting from interactions between existing bars during the fall in water level after floods. We propose that the reworking of bar sediments during low flows (high width‐to‐depth ratio β), reinforced by high values of the Shields mobility parameter, can explain the formation or re‐generation of new alternate migrating bars during a subsequent flood. The migration pattern of the bars was found to be cyclic and to depend mainly on (i) channel layout and (ii) the dynamics of superimposed dunes with heights and lengths depending on location and discharge value. For instance, the hysteresis affecting the steepness of dunes influences the flow resistance of the dunes as well as the celerity of migrating bars during flood events. We compare the findings from the field with results from theoretical studies on alternate bars. This gives insight in the phenomena occurring in the complex setting of real rivers, but it also sheds light on the extent to which bar theories based on idealized cases can predict those phenomena. Copyright © 2014 John Wiley & Sons, Ltd.     

Pearl oysters Pinctada margaritifera grazing on natural plankton in Ahe atoll lagoon (Tuamotu archipelago, French Polynesia)

In atoll lagoons of French Polynesia, growth and reproduction of pearl oysters are mainly driven by plankton concentration. However, the actual diet of black-lip pearl oysters Pinctada margaritifera in these lagoons is poorly known. To fill this gap, we used the flow through chamber method to measure clearance rates of P. margaritifera in Ahe atoll lagoon (Tuamotu Archipelago, French Polynesia). We found: (i) that pearl oysters cleared plankton at a rate that was positively related to plankton biovolume, (ii) that nanoflagellates were the main source of carbon for the pearl oysters, and (iii) that the quantity and origin of carbon filtrated by pearl oysters was highly dependent on the concentration and composition of plankton. These results provide essential elements for the comprehension of growth and reproduction variability of pearl oysters in atoll lagoons of French Polynesia.