Fungal colonies in open fractures of subseafloor basalt

The deep subseafloor crust is one of the few great frontiers of unknown biology on Earth and, still today, the notion of the deep biosphere is commonly based on the fossil record. Interpretation of palaeobiological information is thus central in the exploration of this hidden biosphere and, for each new discovery, criteria used to establish biogenicity are challenged and need careful consideration. In this paper networks of fossilized filamentous structures are for the first time described in open fractures of subseafloor basalts collected at the Emperor Seamounts, Pacific Ocean. These structures have been investigated with optical microscopy, environmental scanning electron microscope, energy dispersive spectrometer, X-ray powder diffraction as well as synchrotron-radiation X-ray tomographic microscopy, and interpreted as fossilized fungal mycelia. Morphological features such as hyphae, yeast-like growth and sclerotia were observed. The fossilized fungi are mineralized by montmorillonite, a process that probably began while the fungi were alive. It seems plausible that the fungi produced mucilaginous polysaccharides and/or extracellular polymeric substances that attracted minerals or clay particles, resulting in complete fossilization by montmorillonite. The findings are in agreement with previous observations of fossilized fungi in subseafloor basalts and establish fungi as regular inhabitants of such settings. They further show that fossilized microorganisms are not restricted to pore spaces filled by secondary mineralizations but can be found in open pore spaces as well. This challenges standard protocols for establishing biogenicity and calls for extra care in data interpretation.     

Ramifications of high in-situ temperatures for laboratory testing and inferred stress states of unlithified sediments – A case study from the Nankai margin

The recovery of drill cores involves changes in pressure and temperature conditions, which inevitably alter the mechanical properties of unlithified sediments. While expansion from unloading after core recovery is well studied, the effects from cooling on standard geotechnical tests are commonly neglected. Along the central portion of the Nankai margin sediments were recovered from high in-situ temperatures of up to 110 °C during IODP Leg 190. So far, the interpretation of the consolidation state of the Lower Shikoku Basin facies (LSB) entering the accretionary Nankai margin is ambiguous. Results from laboratory consolidation tests at room temperature show high pre-consolidation stresses. These were interpreted as hardening caused by cementation, while the field-based porosity vs. depth trend points towards normal consolidation. As an explanation for this discrepancy, the change of the mechanical properties by cooling from in-situ to laboratory conditions is proposed. In this paper, the results of a thermo-mechanical model are compared to published field data. This comparison suggests that the observed hardening is at least partially an artefact from cooling during core recovery, and that the strata may be considered normally consolidated to slightly overconsolidated. The latter can be explained by minor cementation or the influence of secondary consolidation. The results suggest that cooling from high in-situ temperatures may be important for the interpretation of the consolidation state of other sedimentary successions elsewhere.     

Private rights,public benefits: Industry-driven seabed protection

New Zealand has a large exclusive economic zone (EEZ) that contains a variety of marine habitats and commercially-important species. The commercial fishing industry operating within New Zealand's EEZ is of significant value to the economy and fisheries resources are managed through the extensive use of Individual Transferable Quotas (ITQs). One of the benefits of ITQs has been to better align some of the private incentives of quota owners with the public interest. These incentives contributed to an initiative proposed by the fishing industry to close large areas of New Zealand's EEZ to protect the seabed from trawling. These closed areas are termed benthic protection areas (BPAs) and protect the benthic biodiversity of about 1.1 million square kilometres of seabed—approximately 30% of New Zealand's EEZ. A significant proportion of New Zealand's known seamounts and active hydrothermal vents are protected by these closed areas. We describe and discuss the criteria used to select BPAs and some of the criticism of this marine protection initiative. We argue that the assignment of strong property rights in fishing resources was an important precondition to an industry initiative that has a significant public benefit. Where private and public interests are well aligned, government can adopt an enabling and facilitation role, ceding direct control of processes in order to get the results the align with the public interest.     

Induction of vitellogenin in vivo and in vitro in the model teleost medaka (Oryzias latipes): comparison of gene expression and protein levels

Quantification of the egg yolk precursor vitellogenin (VTG) in fish has become a standard technique to detect estrogenic effects of known chemicals and environmental samples. In the present study, we have analysed VTG induction by estradiol, ethynylestradiol and genistein exposure in the model teleost medaka (Oryzias latipes) and demonstrate that the medaka is a suitable model system to analyse estrogenic effects. By comparing VTG gene expression and protein levels we show that in principal both techniques can be used to study VTG induction in vivo (juvenile and adult males) and in vitro (primary cultures of male liver cells). If a short term in vivo or in vitro exposure is performed, detection of mRNA might be sufficient. For long term studies with the need to detect weak estrogenic chemicals and a precise quantification, immuno-chemical detection may be favoured.     

Geological evolution of the West Luzon Basin (South China Sea,Philippines)

Interpretation of deep 2-D multi-channel seismic data sheds insights into the geological evolution of the West Luzon Basin, Philippines. This basin is a sediment-filled trough that is located between the island of Luzon and the outer arc high of the west Luzon subduction zone. High-amplitude, low-frequency reflection bands mark the acoustic basement. The basement, at about 6 s (TWT), is dissected by normal faults with some of them being inverted in a later phase of deformation. The sedimentary successions, overlying the basement are stratified with partly chaotic structures and discontinuous reflectors. Five regional unconformities separate major stratigraphic units. Grid calculations of our seismic data reveal variations in the sedimentation pattern of the basin with a shift of the deposition centre from east to west and backwards during formation. A distinct bottom-simulating reflector is commonly observed. Because the northern boundary of the continental fragments to the South of the West Luzon Basin is unclear we speculate that the basin may be (partly) underlain by continental crust. The continental crust was affected by rifting prior to and during the opening of the South China Sea and the basin was overprinted at a later stage by a forearc structural setting when subduction was initiated.     

Proglacial groundwater storage dynamics under climate change and glacier retreat

Proglacial aquifers are an important water store in glacierised mountain catchments that supplement meltwater-fed river flows and support freshwater ecosystems. Climate change and glacier retreat will perturb water storage in these aquifers, yet the climate-glacier-groundwater response cascade has rarely been studied and remains poorly understood. This study implements an integrated modelling approach that combines distributed glacio-hydrological and groundwater models with climate change projections to evaluate the evolution of groundwater storage dynamics and surface-groundwater exchanges in a temperate, glacierised catchment in Iceland. Focused infiltration along the meltwater-fed Virkisá River channel is found to be an important source of groundwater recharge and is projected to provide 14%–20% of total groundwater recharge by the 2080s. The simulations highlight a mechanism by which glacier retreat could inhibit river recharge in the future due to the loss of diurnal melt cycling in the runoff hydrograph. However, the evolution of proglacial groundwater level dynamics show considerable resilience to changes in river recharge and, instead, are driven by changes in the magnitude and seasonal timing of diffuse recharge from year-round rainfall. The majority of scenarios simulate an overall reduction in groundwater levels with a maximum 30-day average groundwater level reduction of 1 m. The simulations replicate observational studies of baseflow to the river, where up to 15% of the 30-day average river flow comes from groundwater outside of the melt season. This is forecast to reduce to 3%–8% by the 2080s due to increased contributions from rainfall and meltwater runoff. During the melt season, groundwater will continue to contribute 1%–3% of river flow despite significant reductions in meltwater runoff inputs. Therefore it is concluded that, in the proglacial region, groundwater will continue to provide only limited buffering of river flows as the glacier retreats.     

Assessment of agri-spillways as a soil erosion protection measure in Mediterranean sloping vineyards

Suitable vineyard soils enhance soil stability and biodiversity which in turn protects roots against erosion and nutrient losses. There is a lack of information related to inexpensive and suitable methods and tools to protect the soil in Mediterranean sloping vineyards(25° of slope inclination). In the vineyards of the Montes de Málaga(southern Spain), a sustainable land management practice that controls soil erosion is actually achieved by tilling rills in the down-slope direction to canalize water and sediments. Because of their design and use, we call them agri-spillways. In this research, we assessed two agri-spillways(between 10 m and 15 m length, and slopes between 25.8° and 35°) by performing runoff experiments under extreme conditions(a motor driven pump that discharged water flows up to 1.33 l s~(-1) for 12 to 15 minutes: ≈1000 l). The final results showed: i) a great capacity by these rills to canalize large amounts of water and sediments; and, ii) higher water flow speeds(between 0.16 m s-1 and 0.28 m s~(-1)) and sediment concentrationrates(up to 1538.6 g l~(-1)) than typically found in other Mediterranean areas and land uses(such as badlands, rangelands or extensive crops of olives and almonds). The speed of water flow and the sediment concentration were much higher in the shorter and steeper rill. We concluded that agri-spillways, given correct planning and maintenance, can be a potential solution as an inexpensive method to protect the soil in sloping Mediterranean vineyards.     

Comparison of ladderane phospholipid and core lipids as indicators for anaerobic ammonium oxidation (anammox) in marine sediments

We investigated anaerobic ammonium oxidation (anammox) in continental shelf and slope sediments of the Irish and Celtic Seas by using anammox specific ladderane biomarker lipids. We used the presence of an intact ladderane phospholipid as a direct indicator for living anammox bacteria, and compared it with the abundance of ladderane core lipids derived from both living and dead bacterial biomass. All investigated sediments contained ladderane core lipids as well as the intact ladderane phospholipid, in agreement with ¹⁵N-labeling experiments, which revealed anammox activity at all sites. Ladderane core lipid and intact ladderane phospholipid concentrations were significantly correlated (R² = 0.957 and 0.464, respectively) with anammox activity over the transect of the continental shelf and slope sediments. In the Irish Sea (50-100 m water depth) highest abundances of the intact ladderane phospholipid were found in the upper 2 cm of the sediment, indicating a zone of active anammox. A sharp decline further down-core suggested a strong decrease in anammox biomass and rapid degradation of the intact lipids. In comparison, ladderane core lipids were 1-2 orders of magnitude higher in concentration than the intact ladderane phospholipid and accumulated as dead cell remnants with depth. In the slope sediments of the Celtic Sea both ladderane core lipids and the intact ladderane phospholipid were found in sediments at water depths ranging from 500 to 2000 m. Here, anammox seemed to be active at greater depths of the sediment (>2 cm). Mean abundances of both intact and core ladderane lipids in whole sediment cores increased downslope, indicating an increasing importance of anammox in deeper slope sediments.