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摘要:目的 探讨菌株Salinivibrio sp.YH4分泌的丝氨酸蛋白酶EYHS的耐盐性及结构特征。方法 明胶底物酶谱法分析EYHS的耐盐性。应用生物信息学手段对EYHS及6种耐盐的S8家族丝氨酸蛋白酶结构特征进行分析。结果 EYHS在4 mol/L的NaCl溶液中仍具有活性,属于耐盐蛋白酶。EYHS及6种S8家族丝氨酸蛋白酶分子表面的loop区等无规则卷曲所占比例较高,α-螺旋与β-片层则主要位于酶分子内部。EYHS分子表面酸性氨基酸含量较高,且具有弱疏水内核。多序列比对发现蛋白酶的催化三联体两侧存在高度保守的基序和保守的极性氨基酸及芳香族氨基酸,并存在多个保守的Gly与Ala。同源模建和表面电荷分布显示,α螺旋和β片层围成了蛋白酶的催化腔,EYHS活性中心包含由Asp32、His65与Ser215组成的催化三联体,且催化位点区域表面静电势为负。结论 上述结构特征可能有助于耐盐丝氨酸蛋白酶EYHS在高盐环境下维持其稳定性和适度柔性,并有助于其催化功能的发挥,为深入研究耐盐丝氨酸蛋白酶的高盐环境适应性提供了一定的理论依据。 相似文献
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金属矿产资源的持续开发活动通常会导致矿区土壤重金属含量累积,厘清土壤重金属环境污染特征并评价其生态风险,可为监测土壤环境质量和预防土壤污染提供依据。选择江西于都某百年开采钨矿山,采用单因子污染指数法、内梅罗综合指数法和Hakanson潜在生态风险指数法,对其周边土壤中Cr、Ni、Cu、Zn、Cd、Pb、As、Hg污染特征及潜在生态风险进行调查评价。评价结果表明,研究区土壤受到Cu、Zn、Cd、Pb、As等重金属的污染,单因子污染指数分别为6.74、3.72、45.1、3.36、8.88;综合污染评价表明,Cd是主要污染贡献因子,其次为As、Cu、Pb、Zn;土壤综合潜在生态风险指数平均值高达2065,属很强生态风险;强生态风险及以上区域主要分布在矿山周边及矿山下游河道附近,分布面积约占研究区总面积的43.89%;研究区土壤重金属相关性分析表明,Cu、Zn、Cd、Pb之间具有显著的相关性。综合研究结果可知,该区域土壤Cd具有一定的潜在生态风险,应引起充分重视,矿山开发利用可能是导致Cu、Zn、Cd、Pb污染的主要原因。 相似文献
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冬季积雪对我国夏季降水预测的评估分析 总被引:7,自引:2,他引:7
根据高原积雪和高纬积雪与我国夏季降水相关分析的结果,将高原积雪和高纬积雪作为独立因子分别对我国夏季降水预测做了检验,结果表明:高原积雪较高纬积雪效果要好,冬季高原积雪异常偏多时,长江流域夏季易发生洪涝,这也是预测汛期降水的一个重要信号。 相似文献
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Song Guo-xuan Yan Hao-jian Xu Tong-qi Zhu Shi-chang 《Chinese Astronomy and Astrophysics》1981,5(3):332-336
We have found an interior solution of the Einstein equation containing a vector graviton field under a special mass distribution. 相似文献
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Distinct groundwater recharge sources and geochemical evolution of two adjacent sub-basins in the lower Shule River Basin,northwest China 总被引:1,自引:1,他引:1
Liheng Wang Yanhui Dong Yueqing Xie Fan Song Yaqiang Wei Jiangyi Zhang 《Hydrogeology Journal》2016,24(8):1967-1979
Based on analysis of groundwater hydrogeochemical and isotopic data, this study aims to identify the recharge sources and understand geochemical evolution of groundwater along the downstream section of the Shule River, northwest China, including two sub-basins. Groundwater samples from the Tashi sub-basin show markedly depleted stable isotopes compared to those in the Guazhou sub-basin. This difference suggests that groundwater in the Tashi sub-basin mainly originates from meltwater in the Qilian Mountains, while the groundwater in the Guazhou sub-basin may be recharged by seepage of the Shule River water. During the groundwater flow process in the Tashi sub-basin, minerals within the aquifer material (e.g., halite, calcite, dolomite, gypsum) dissolve in groundwater. Mineral dissolution leads to strongly linear relationships between Na+ and Cl? and between Mg2++ Ca2+ and SO4 2??+?HCO3 ?, with stoichiometry ratios of approximately 1:1 in both cases. The ion-exchange reaction plays a dominant role in hydrogeochemical evolution of groundwater in the Guazhou sub-basin and causes a good linear relationship between (Mg2++ Ca2+)–(SO4 2??+?HCO3 ?) and (Na++ K+)–Cl? with a slope of ?0.89 and also results in positive chloroalkaline indices CAI 1 and CAI 2. The scientific results have implications for groundwater management in the downstream section of Shule River. As an important irrigation district in Hexi Corridor, groundwater in the Guazhou sub-basin should be used sustainably and rationally because its recharge source is not as abundant as expected. It is recommended that the surface water should be used efficiently and routinely, while groundwater exploitation should be limited as much as possible. 相似文献
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Yanyan Liu Yidong Lou Shirong Ye Rui Zhang Weiwei Song Xing Zhang Qingquan Li 《GPS Solutions》2017,21(4):1647-1659
Although integer ambiguity resolution (IAR) can improve positioning accuracy considerably and shorten the convergence time of precise point positioning (PPP), it requires an initialization time of over 30 min. With the full operation of GLONASS globally and BDS in the Asia–Pacific region, it is necessary to assess the PPP–IAR performance by simultaneous fixing of GPS, GLONASS, and BDS ambiguities. This study proposed a GPS + GLONASS + BDS combined PPP–IAR strategy and processed PPP–IAR kinematically and statically using one week of data collected at 20 static stations. The undifferenced wide- and narrow-lane fractional cycle biases for GPS, GLONASS, and BDS were estimated using a regional network, and undifferenced PPP ambiguity resolution was performed to assess the contribution of multi-GNSSs. Generally, over 99% of a posteriori residuals of wide-lane ambiguities were within ±0.25 cycles for both GPS and BDS, while the value was 91.5% for GLONASS. Over 96% of narrow-lane residuals were within ±0.15 cycles for GPS, GLONASS, and BDS. For kinematic PPP with a 10-min observation time, only 16.2% of all cases could be fixed with GPS alone. However, adding GLONASS improved the percentage considerably to 75.9%, and it reached 90.0% when using GPS + GLONASS + BDS. Not all epochs could be fixed with a correct set of ambiguities; therefore, we defined the ratio of the number of epochs with correctly fixed ambiguities to the number of all fixed epochs as the correct fixing rate (CFR). Because partial ambiguity fixing was used, when more than five ambiguities were fixed correctly, we considered the epoch correctly fixed. For the small ratio criteria of 2.0, the CFR improved considerably from 51.7% for GPS alone, to 98.3% when using GPS + GLONASS + BDS combined solutions. 相似文献