Salinivibrio sp.YH4胞外丝氨酸蛋白酶EYHS耐盐性及生物信息学分析

摘要：目的 探讨菌株Salinivibrio sp.YH4分泌的丝氨酸蛋白酶EYHS的耐盐性及结构特征。方法 明胶底物酶谱法分析EYHS的耐盐性。应用生物信息学手段对EYHS及6种耐盐的S8家族丝氨酸蛋白酶结构特征进行分析。结果 EYHS在4 mol/L的NaCl溶液中仍具有活性,属于耐盐蛋白酶。EYHS及6种S8家族丝氨酸蛋白酶分子表面的loop区等无规则卷曲所占比例较高,α-螺旋与β-片层则主要位于酶分子内部。EYHS分子表面酸性氨基酸含量较高,且具有弱疏水内核。多序列比对发现蛋白酶的催化三联体两侧存在高度保守的基序和保守的极性氨基酸及芳香族氨基酸,并存在多个保守的Gly与Ala。同源模建和表面电荷分布显示,α螺旋和β片层围成了蛋白酶的催化腔,EYHS活性中心包含由Asp32、His65与Ser215组成的催化三联体,且催化位点区域表面静电势为负。结论 上述结构特征可能有助于耐盐丝氨酸蛋白酶EYHS在高盐环境下维持其稳定性和适度柔性,并有助于其催化功能的发挥,为深入研究耐盐丝氨酸蛋白酶的高盐环境适应性提供了一定的理论依据。     

中国沿海小黄鱼种群动力学及其空间异质性研究

小黄鱼是我国近海四种最重要的经济鱼类之一,在过去的几十年中小黄鱼种群及其两个地理亚种群经历了巨大的变化。小黄鱼的种群动力学研究,对于维持这一重要渔业的可持续管理是至关重要的。目前仅有的两个小黄鱼种群动力学模型只涵盖了较短的时间,且没有关注其空间差异。本文研究了1968年至2015年间黄渤海和东海小黄鱼的种群动力学模型,包含和不包含空间分层结构的两种贝叶斯模型被用于分析其种群动力学的大尺度空间异质性。本文分析了不同的假设,来研究小黄鱼种群动力学潜在的变化趋势。研究结果表明小黄鱼种群动力学特征具有明显的时间和空间变化。种群的增长速度从20世纪八十年代开始增加,而可捕系数从1981年到2015年增加了两倍多。与黄渤海亚种群相比,东海的小黄鱼亚种群生长速度更快,遭受的捕捞压力也更大。基于最大可持续产量MSY的参考点表明,无论是整个小黄鱼种群还是两个亚种群,近年来都有非常高的过度捕捞风险。因此我国小黄鱼的渔业管理急需更加保守的管理策略,同时考虑其地域差异。本文所用的方法可以应用于其他种类的资源评估和渔业管理,尤其是具有空间异质性和数据有限的种类。     

Multiple Unmanned Underwater Vehicles Consensus Control with Unmeasurable Velocity Information and Environmental Disturbances Under Switching Directed Topologies

China Ocean Engineering - A consensus algorithm proposed in the paper is applied to tackle remarkable problems of unmeasurable velocities, the environmental disturbances, and the limited...     

基于功能视角的广州都市边缘区乡村发展类型分化及其动力机制

基于村庄规划详细调查数据库,采用空间集中度和功能识别等方法,深度剖析广州都市边缘区村域尺度乡村发展的类型分化及其动力机制.结果 表明:①广州都市边缘区村域乡村功能的空间梯度差异特征明显.乡村经济发展功能和社会保障功能呈现出由城区向城郊逐渐减弱的分异特征,农业生产功能和生态保育功能则由城区向城郊逐渐增强态势.以乡村主导功...     

Perceptions of Land Tenure Security in Rural China: The Impact of Land Reallocations and Certification

Abstract

Tenure security is commonly recognized as an important factor in stimulating long-term investments in land. Recent studies suggest that a distinction between legal, actual and perceived tenure security needs to be made in analyzing tenure security. This study discusses the relationships between legal, actual and perceived land tenure security in rural China, and empirically examines the impact of actual on perceived land tenure security by applying Probit models to household and village survey data collected in four provinces. Using household expectations about the absence of land reallocations within the next five years as the dependent variable, we find that tenure security is positively affected by the possession of land certificates in villages that periodically reallocated land but not in villages that did not do so. The estimated impact is larger for land certificates issued in the new round of land certification than for land certificates that were issued earlier.     

耀变体在射电波段的偏振研究

利用UMRAO数据库22个耀变体源的偏振数据,根据Lazarian&Pogosyan理论模型分析研究了耀变体射电波段的偏振随波长的变化,得出不同类的耀变体偏振随波长变化的原因。结果表明,与Lazarian&Pogosyan理论模型吻合较好。由此得出如下结论:(1)反常去偏振耀变体源中反常法拉第旋光起主导作用,反常去偏振相对较少;(2)热辐射与同步辐射混合作用,高频波段的偏振度小于低频波段的偏振度(反常去偏振);(3)当被观测波段波长小于吸积盘热辐射对应的维恩波长时,高频波段的偏振度大于低频波段的偏振度(常规去偏振)。     

中国县域乡村地域多功能格局及影响因素识别

论文以中国大陆县域为研究单元,构建乡村地域多功能评价指标体系,利用熵权法、半变异函数和地理探测器等模型,对2000—2015年中国乡村多功能指数进行综合测评,揭示中国县域乡村地域多功能的空间分异特征,定量识别乡村地域多功能空间分异的影响因素。研究结果表明：① 中国乡村地域多功能在空间上呈现出平原、东部沿海等地区高值集聚,高原、山地等地区低值集聚,各县域乡村地域多功能整体呈现出逐渐提升态势;② 经济发展功能和社会保障功能对乡村地域多功能的贡献率逐渐增大,农业生产功能和生态保育功能对乡村地域多功能的贡献率逐渐减小;③ 2000—2015年,中国大陆范围内县域乡村地域多功能空间自相关范围和强度总体呈现减小的趋势,随机性因子成为乡村地域多功能空间分异的主要驱动力;④ 县域经济整体发展水平和财政收入是影响乡村地域多功能空间分异的主导因素;各影响因素之间的两两交互作用会增强乡村地域多功能的空间分异;社会环境因素对乡村地域多功能空间分异的影响程度逐渐上升,自然环境因素的影响程度逐渐下降。     

Rural settlement spatial patterns and effects: Road traffic accessibility and geographic factors in Guangdong Province,China

Journal of Geographical Sciences - Based on land use classification data of remote sensing images, using kernel density, the minimal cumulative resistance model of road traffic accessibility, and a...     

Multiscale geographically and temporally weighted regression: exploring the spatiotemporal determinants of housing prices

Understanding scale effects is important and indispensable for geography studies. However, spatial and spatiotemporal statistical tools for measuring the operational scales of different processes are rather limited. This article extends the popular geographically and temporally weighted regression (GTWR) model to consider operational scale effects by proposing multiscale GTWR (MGTWR), which offers a flexible and scalable framework for identifying and analysing multiscale processes by specifying flexible bandwidths for various covariates. Then, MGTWR is employed to explore spatiotemporal variations and how influential factors are associated with housing prices in Shenzhen. This article attempts to extend GTWR to MGTWR in consideration of scale effects, thereby highlighting the importance of different levels of spatiotemporal heterogeneity. Furthermore, the empirical results of this study can provide valuable policy implications for real estate development in areas where urban planning should address multiscale effects in both temporal and spatial dimensions.     

Rift structures and its related unconformities on and adjacent the Dongsha Rise: insights into the nature of the high-velocity layer in the northern South China Sea

Marine Geophysical Research - It is curious that the typical feature of high-velocity layer (HVL) underneath the crust for the magma-rich margin was found in the magma-poor margin of the South...