白缘(鱼央)5S rDNA的扩增分析及其在染色体上的FISH定位

本文分别对雌雄白缘(鱼央)的5S rDNA进行了PCR扩增和序列分析,并采用双色荧光原位杂交(FISH)技术,以白缘(鱼央)的5S rDNA序列和小麦的45S rDNA为探针,对其在白缘(鱼央)雌雄中期染色体上进行了FISH定位研究。结果表明,白缘(鱼央)5S rDNA序列无雌雄差异;5S rDNA的保守区序列为117 bp;5S rDNA和45S rDNA分别被定位于白缘(鱼央)的性染色体和第5号染色体上。同时从GenBank中获得了22种鱼的5S rDNA,运用DNAman软件构建了23种鱼的系统发育树,对白缘(鱼央)的进化地位进行了初步研究。本研究为阐明白缘(鱼央)在鱼类系统进化中的位置、重复序列在脊椎动物性染色体上的分布状况以及与性别决定与分化的关系,提供了资料积累和分析依据。     

附着基粗糙度等因素与海藻密度的相关性研究

海藻附着基表面粗糙度与孢子附着密度有密切的相关性;已经由许多研究证实。但是海藻苗的密度是否仍然受到附着基粗糙度的影响;还缺乏明确的结论。本文以尼龙和亚克力两种材料作为海藻附着基;悬挂于自然海区中使海藻孢子附着并萌发生长;用以研究附着基表面粗糙度对4种海藻密度的影响。结果显示;多管藻、尾孢藻、点叶藻、硬毛藻这4种海藻;在不具沟槽的光滑面;密度显著低于任何有沟槽的粗糙面;但粗糙度不同的附着基之间比较;对海藻密度的影响并不显著。室内培育的海带苗;在尼龙上的密度与粗糙度成正相关;但是将海带苗移至海上培育后;不再与粗糙度有相关性;可能与其假根状固着器下海后的发育有关。本文还试验了海泥、灭活海泥和低栖硅藻3种沉积物与附着基粗糙度对海藻密度的影响;结果表明;沉积物和附着基粗糙度;以及它们的交互作用对海藻密度的影响;因附着基的材料和海藻种类而有差别。     

Climate–growth relationship stability of <Emphasis Type="Italic">Picea crassifolia</Emphasis> on an elevation gradient,Qilian Mountain,Northwest China

Climate affects Picea crassifolia growth and climate change will lead to changes in the climate–growth relationship (i.e., the “divergence” phenomenon). However, standardization methods can also change the understanding of such a relationship. We tested the stability of this relationship by considering several variables: 1) two periods (1952–1980 and 1981–2009), 2) three elevations (2700, 3000, and 3300 m), and 3) chronologies detrended using cubic splines with two different flexibilities. With increasing elevation, the climatic factor limiting the radial growth of Picea crassifolia shifted from precipitation to temperature. At the elevation of 2700 m, the relationship between radial growth and mean temperature of the previous December changed so that the more flexible spline had a greater precipitation signal. At the elevation of 3000 m, positive correlation of radial growth with mean temperature and precipitation in September of the previous year became more significant. At the elevation of 3300 m, positive correlation between radial growth and precipitation of the current summer and the previous spring and autumn was no longer significant, whereas the positive correlation between radial growth and temperature of the current spring and summer strengthened. The detrending with the most flexible spline enhanced the precipitation signal at 2700 m, while that with the least flexible spline enhanced the temperature signal at 3300 m. All results indicated that the divergence phenomenon was affected by the climatic signals in the chronologies and that it was most dependent on the detrending method. This suggests it is necessary to select a suitable spline bootstrap for studies of growth divergence phenomena.     

Application of 2D numerical model to unsteady performance evaluation of vertical-axis tidal current turbine

Tidal current energy is renewable and sustainable, which is a promising alternative energy resource for the future electricity supply. The straight-bladed vertical-axis turbine is regarded as a useful tool to capture the tidal current energy especially under low-speed conditions. A 2D unsteady numerical model based on Ansys-Fluent 12.0 is established to conduct the numerical simulation, which is validated by the corresponding experimental data. For the unsteady calculations, the SST model, 2×10~5 and 0.01 s are selected as the proper turbulence model, mesh number, and time step, respectively. Detailed contours of the velocity distributions around the rotor blade foils have been provided for a flow field analysis. The tip speed ratio(TSR) determines the azimuth angle of the appearance of the torque peak, which occurs once for a blade in a single revolution. It is also found that simply increasing the incident flow velocity could not improve the turbine performance accordingly. The peaks of the averaged power and torque coefficients appear at TSRs of 2.1 and 1.8, respectively. Furthermore, several shapes of the duct augmentation are proposed to improve the turbine performance by contracting the flow path gradually from the open mouth of the duct to the rotor. The duct augmentation can significantly enhance the power and torque output. Furthermore, the elliptic shape enables the best performance of the turbine. The numerical results prove the capability of the present 2D model for the unsteady hydrodynamics and an operating performance analysis of the vertical tidal stream turbine.     

Depth classification of underwater targets based on complex acoustic intensity of normal modes

In order to solve the problem of depth classification of the underwater target in a very low frequency acoustic field, the active component of cross spectra of particle pressure and horizontal velocity (ACCSPPHV) is adopted to distinguish the surface vessel and the underwater target. According to the effective depth of a Pekeris waveguide, the placing depth forecasting equations of passive vertical double vector hydrophones are proposed. Numerical examples show that when the sum of depths of two hydrophones is the effective depth, the sign distribution of ACCSPPHV has nothing to do with horizontal distance; in addition, the sum of the first critical surface and the second critical surface is equal to the effective depth. By setting the first critical surface less than the difference between the effective water depth and the actual water depth, that is, the second critical surface is greater than the actual depth, the three positive and negative regions of the whole ocean volume are equivalent to two positive and negative regions and therefore the depth classification of the underwater target is obtained. Besides, when the 20 m water depth is taken as the first critical surface in the simulation of underwater targets (40 Hz, 50 Hz, and 60 Hz respectively), the effectiveness of the algorithm and the correctness of relevant conclusions are verified, and the analysis of the corresponding forecasting performance is conducted.