利用ITS-5.8S rDNA序列对2株海洋亚历山大藻进行的分子鉴定

扩增2株分离自青岛胶州湾、从形态上初步确定为亚历山大藻属(Alexandrium sp. qd2, Alexandrium sp. qd3)的5.8S 核糖体基因(5.8S rDNA), 转录单元内间隔区(Internal Transcribed Spacer, ITS) 序列、部分18S rDNA和28S rDNA序列.通过blastn比对、系统进化树以及遗传距离分析将A.sp.qd2和A.sp.qd3鉴定为A.catenella,且属于A.catenella 的不同株型.     

Analysis of stochastic characteristics of the Benue River flow process

Stochastic characteristics of the Benue River streamflow process are examined under conditions of data austerity. The streamflow process is investigated for trend, non-stationarity and seasonality for a time period of 26 years. Results of trend analyses with Mann-Kendall test show that there is no trend in the annual mean discharges. Monthly flow series examined with seasonal Kendall test indicate the presence of positive change in the trend for some months, especially the months of August, January, and February. For the stationarity test, daily and monthly flow series appear to be stationary whereas at 1%, 5%, and 10% significant levels, the stationarity alternative hypothesis is rejected for the annual flow series. Though monthly flow appears to be stationary going by this test, because of high seasonality, it could be said to exhibit periodic stationarity based on the seasonality analysis. The following conclusions are drawn： （1） There is seasonality in both the mean and variance with unimodal distribution. （2） Days with high mean also have high variance. （3） Skewness coefficients for the months within the dry season period are greater than those of the wet season period, and seasonal autocorrelations for streamflow during dry season are generally larger than those of the wet season. Precisely, they are significantly different for most of the months. （4） The autocorrelation functions estimated ＂over time＂ are greater in the absolute value for data that have not been deseasonalised but were initially normalised by logarithmic transformation only, while autocorrelation functions for i = 1, 2 365 estimated ＂over realisations＂ have their coefficients significantly different from other coefficients.     

Preliminary comparison of trace metals in coastal aerosols between Qingdao and Liverpool

Aerosol samples were collected at two coastal suburban stations, Qingdao （China） in 1995-1996 and Liverpool （U.K.） in 1995, respectively. The samples were analyzed to determine the concentrations of trace metals （Cr, Zn, Cu, Co, Ni, Pb, V, and Cd） as well as A1, Fe and Mn. Data were examined to understand the difference of trace metals in aerosols between coastal zones downwind the developing area （near the Yellow Sea） and developed region （near the Irish Sea）. The results show that most elements at Qingdao have levels 4-5 times higher than those at Liverpool, particularly for the crust-dominated elements （e.g. Al, Fe and Mn）. Moreover, the aerosol composition at Qingdao is higher in spring than in summer, underlying the influence of westerlies and local emissions in combination, whereas seasonal change of aerosol composition is not significant at Liverpool. The enrichment factors for the crustal source elements （EFcrust） at Liverpool are much higher than those at Qingdao. The contributions from the pollutant source （Rp） for some trace metals like Cu, Pb, Zn and Cd are 〉90% at Qingdao and Liverpool, suggesting overwhelming anthropogenic contributions to these metals. The contributions from crustal source （Re） for trace metals tend to increase with higher aerosol levels and Al concentration at Qingdao, indicating a good correlation between the crust-dominated component and the air mass. At Liverpool, the Rc values for trace metals are positively correlated with Al concentrations instead of with aerosol mass, suggesting that Al in aerosols represents the crustal component even though the aerosols come from different sources.     

Construction of bacterial artificial chromosome libraries for Zhikong Scallop <Emphasis Type="Italic">Chlamys farreri</Emphasis>

Two Large-insert genomic bacterial artificial chromosome （BAC） libraries of Zhikong scallop Chlamys farreri were constructed to promote our genetic and genomic research. High-quality megabase-sized DNA was isolated from the adductor muscle of the scallop and partially digested by BamH I and Mbo I, respectively. The BamH I library consisted of 53 760 clones while the Mbo I library consisted of 7 680clones. Approximately 96 % of the clones in BamH I library contained nuclear DNA inserts in average size of 100 kb, providing a coverage of 5.3 haploid genome equivalents. Similarly, the Mbo I library with an average insert of 145 kb and no insert-empty clones, thus providing a genome coverage of 1.1 haploid genome equivalents.     

Wave breaking on turbulent energy budget in the ocean surface mixed layer

As an important physical process at the air-sea interface, wave movement and breaking have a significant effect on the ocean surface mixed layer （OSML）. When breaking waves occur at the ocean surface, turbulent kinetic energy （TKE） is input downwards, and a sublayer is formed near the surface and turbulence vertical mixing is intensively enhanced. A one-dimensional ocean model including the Mellor-Yamada level 2.5 turbulence closure equations was employed in our research on variations in turbulent energy budget within OSML. The influence of wave breaking could be introduced into the model by modifying an existing surface boundary condition of the TKE equation and specifying its input. The vertical diffusion and dissipation of TKE were effectively enhanced in the sublayer when wave breaking was considered. Turbulent energy dissipated in the sublayer was about 92.0% of the total depth-integrated dissipated TKE, which is twice higher than that of non-wave breaking. The shear production of TKE decreased by 3.5% because the mean flow fields tended to be uniform due to wave-enhanced turbulent mixing. As a result, a new local equilibrium between diffusion and dissipation of TKE was reached in the wave-enhanced layer. Below the sublayer, the local equilibrium between shear production and dissipation of TKE agreed with the conclusion drawn from the classical law-of-the-wall （Craig and Banner, 1994）.     

Effects of diet and body size on phosphorus utilization of Liza haematocheila T. ＆ S.

A 21-d laboratory experiment was conducted to study, the phosphorus （P） utilization of two different diets by redlip mullet Liza haematocheila T. ＆ S. Sand-filtered water in salinity 30 and temperatare 25℃ was used. Twenty-nine fish individuals were divided into three groups： 11 to group 1 （G1） fed on diet 1, 11 to group 2 （G2） fed on diet 2, and 7 to contrast group. Diet 1 was a commercial feed, more valuable in nutrition than diet 2 that similar to natural detritus. The results show the intake phosphorus （IP） of G1 was significantly higher than that of G2, and both increased linearly with body size at a certain amount of diet. The retention phosphorus （RP） in fish of G1 was lower than G2. The relationship between retention phosphorus and body size was positive and stronger in G2. Significant difference in faecal phosphorus （FP） was found between G1 and G2. Body size significantly impacted the excretion phosphorus （EP） in G1 but G2. The loss of intake phosphorus in G1 was 10.83-20.27 mg per g fish weight gain, higher than that in G2 for 6.63-9.56. Of the phosphorus, about 10% was allocated into growth, 50% in faeces, and the rest lost in excretion. The main part of phosphorus was lost in faeces but excretion. The phosphorus budget of the fish could be described as 100IP = 7.40RP ＋ 47.39FP ＋ 36.63EP （Diet 1） or 100IP = 11.93RP ＋ 56.64FP ＋ 21.76EP （Diet 2）.     

Molecular phylogenetic analysis of attached Ulvaceae species and free-floating <Emphasis Type="Italic">Enteromorpha</Emphasis> from Qingdao coasts in 2007

Based on the sequence data of the nuclear ribosomal DNA internal transcribed spacer (ITS) 1, 5.8 S, and ITS 2, the molecular phylogeny was analyzed on Ulvaceae species collected from Qingdao coasts in summer of 2007, including 15 attached Ulva and Enteromorpha samples from 10 locations and 10 free-floating Enteromorpha samples from seven locations. The result supported the monophyly of all free-floating Enteromorpha samples, implying the unialgal composition of the free-floating Enteromorpha, and the attached Ulvaceae species from Qingdao coasts were grouped into other five clades, suggesting that they were not the biogeographic origin of the free-floating Enteromorpha in that season. Supported by NSFC (40506030); the Innovative Key Project of the Chinese Academy of Sciences (KZCX2-YW-209); Science & Technology Project of Qingdao City (06-2-2-12-JCH)     

Cloning and sequence analysis of a full-length cDNA of <Emphasis Type="Italic">SmPP1cb</Emphasis> encoding turbot protein phosphatase 1 beta catalytic subunit

Reversible protein phosphorylation, catalyzed by protein kinases and phosphatases, is an important and versatile mechanism by which eukaryotic cells regulate almost all the signaling processes. Protein phosphatase 1 （PP1） is the first and well-characterized member of the protein serine/threonine phosphatase family. In the present study, a full-length cDNA encoding the beta isoform of the catalytic subunit of protein phosphatase l（PPlcb）, was for the first time isolated and sequenced from the skin tissue of flatfish turbot Scophthalmus maximus, designated SmPPlcb, by the rapid amplification of cDNA ends （RACE） technique. The cDNA sequence of SmPPlcb we obtained contains a 984 bp open reading frame （ORF）, flanked by a complete 39 bp 5＇ untranslated region and 462 bp 3＇ untranslated region. The ORF encodes a putative 327 amino acid protein, and the N-terminal section of this protein is highly acidic, Met-Ala-Glu-Gly-Glu-Leu-Asp-Val-Asp, a common feature for PP1 catalytic subunit but absent in protein phosphatase 2B （PP2B）. And its calculated molecular mass is 37 193 Da and pI 5.8. Sequence analysis indicated that, SmPPlcb is extremely conserved in both amino acid and nucleotide acid levels compared with the PPlcb of other vertebrates and invertebrates, and its Kozak motif contained in the 5＇UTR around ATG start codon is GXXAXXGXXATGG, which is different from mammalian in two positions A6 and G3, indicating the possibility of different initiation of translation in turbot, and also the 3＇UTR of SmPPlcb is highly diverse in the sequence similarity and length compared with other animals, especially zebraf＇lsh. The cloning and sequencing of SmPPlcb gene lays a good foundation for the future work on the biological functions of PP1 in the flatfish turbot.     

Singularly perturbed solution of coupled model in atmosphere-ocean for global climate

A box model of the interhemispheric thermohaline circulation （THC） in atmosphere-ocean for global climate is considered. By using the multi-scales method, the asymptotic solution of a simplified weakly nonlinear model is discussed. Firstly, by introducing first scale, the zeroth order approximate solution of the model is obtained. Secondly, by using the multi-scales, the first order approximate equation of the model is found. Finally, second order approximate equation is formed to eliminate the secular terms, and a uniformly valid asymptotic expansion of solution is decided. The multi-scales solving method is an analytic method which can be used to analyze operation sequentially. And then we can also study the diversified qualitative and quantitative behaviors for corresponding physical quantities. This paper aims at providing a valid method for solving a box model of the nonlinear equation.     

Sensitivity of penman-monteith reference crop evapotranspiration in Tao’er River Basin of northeastern China

A non-dimensional relative sensitivity coefficient was employed to predict the responses of reference crop evapotranspiration （ET0） to perturbation of four climate variables in Tao＇er River Basin of the northeastern China. Mean monthly ET0 and yearly ET0 from 1961 to 2005 were estimated with the FAO-56 Penman-Monteith Equation. A 45-year historical dataset of average monthly maximum/minimum air temperature, mean air temperature, wind speed, sunshine hours and relative humidity from 15 meteorological stations was used in the analysis. Results show that： 1） Sensitivity coefficients of wind speed, air temperature and sunshine hours were positive except for those of air temperature of Arxan Meteorological Station, while those of relative humidity were all negative. Relative humidity was the most sensitive variable in general for the Tao＇er River Basin, followed by sunshine hours, wind speed and air temperature. 2） Similar to climate variable, monthly sensitivity coefficients exhibit large annual fluctuations. 3） Sensitivity coefficients for four climate variables all showed significant trends in seasonal/yearly series. Also, sensitivity coefficients of air temperature, sunshine hours and wind speed all showed significant trends in spring. 4） Among all sensitivity coefficients, the average yearly sensitivity coefficient of relative humidity was highest throughout the basin and showed largest spatial variability. Longitudinal distribution of sensitivity coefficients for air temperature, relative humidity and sunshine hours was also found, which was similar to the distribution of the three climate variables.