Phylogeography of the rocky intertidal periwinkle Echinolittorina paytensis through a biogeographic transition zone in the Southeastern Pacific

Barriers to dispersal are recognized to play an important role in the differentiation of populations and ultimately in speciation. In the southeast Pacific, on the northern coast of Peru, a transition zone between the Peruvian and Panamic marine biogeographic provinces exists. Here, the convergence between two contrasting surface currents could generate a barrier effect for the larval dispersal of meroplanktonic invertebrates, which could in turn generate differentiated populations or genetic lineages on both sides of the transition zone. To address this, we studied to Echinolittorina paytensis, an abundant rocky intertidal periwinkle that spans both biogeographic provinces. A total of 95 individuals from Ecuador (2°19′S) to central Peru (7°31′S), covering the Panamic Province, the Peruvian Province, and the transition zone between, were collected. The mitochondrial markers cytochrome c oxidase I and 16SrRNA were sequenced in order to investigate phylogeography and genetic structuring. In general, no genetic structuring was found across the transition zone, suggesting this biogeographic boundary would not be acting as a barrier in this species. Factors such as a high larval dispersal capability and the occurrence of El Niño–Southern Oscillation events such as El Niño are discussed.     

Extracts of the seaweed Bifurcaria bifurcata display antifungal activity against human dermatophyte fungi

Bifurcaria bifurcata is a seaweed of the Order Fucales(Ochrophyta, Phaeophyceae) that can be found all year round along the Portuguese Atlantic shore. Although it is considered edible in some countries,its biotechnological potential has not yet been assessed in detail and here we report its antimicrobial potential against human dermatophyte fungi. Three samples were harvested from Baleal Island(Peniche, Portugal)harvested in winter(BbPe); from Aguda Beach(Arcozelo, Vila Nova de Gaia, Portugal), harvested in autumn(BbAg) and a sample obtained by culturing the Aguda sample under laboratory conditions(BbLC).A broth macrodilution assay was applied to determine the MIC(minimum inhibitory concentration) and MLC(minimum lethal concentration) of the B. bifurcata extracts. Results show that the BbPe MeOH extracts were the most effective and had higher antifungal activity against all the tested dermatophyte strains than the BbLC and BaAg extracts [Epidermophyton floccosum FF9(100-200 μg/mL MIC; 200 μg/mL MLC), Microsporum canis FF1(400 μg/mL MIC; 400 μg/mL MLC), Trichophyton mentagrophytes FF7(100 μg/mL MIC; 800 μg/mL MLC), M. gypseum CECT 2908(800 μg/mL MIC; 800 μg/mL MLC), T.mentagrophytes var. interdigitale CECT 2958(800 μg/mL MIC; 800 μg/mL MLC), T. rubrum CECT 2794(200 μg/mL MIC; 400 μg/mL MLC)]. In fact, only E. floccosum FF9 was sensitive to BbAg and BbLC MeOH extracts. To our knowledge, this is the first report of antifungal activity of B. bifurcata against human dermatophyte fungi.     

Multi‐scale analysis of a migrating submarine channel system in a tectonically‐confined basin: The Miocene Gorgoglione Flysch Formation,southern Italy

The Miocene Gorgoglione Flysch Formation records the stratigraphic product of protracted sediment transfer and deposition through a long‐lived submarine channel system developed in a narrow and elongate thrust‐top basin of the Southern Apennines (Italy). Channel‐fill deposits are exposed in an outcrop belt approximately 500 m thick and 15 km long, oriented oblique to the palaeoflow, which was roughly south‐eastward. These exceptional exposures of channel‐fill strata allow the stacking architectures and the evolution of the channel system to be analyzed at multiple scales, enabling the effects of syn‐sedimentary thrust tectonics and basin confinement on the depositional system development to be deciphered. Two end‐member types of elementary channel architecture have been identified: high‐aspect‐ratio, weakly‐confined channels, and low‐aspect‐ratio, incisional channels. Their systematic stacking results in a complex pattern of seismic‐scale depositional architectures that determines the stratigraphic framework of the deep‐water system. From the base of the succession, two prominent channel complex sets have been recognized, namely CS1 and CS2, consisting of amalgamated incisional channel elements and weakly‐confined channel elements. These channelized units are overlain by isolated incisional channels, erosional into mud‐prone slope deposits. The juxtaposition of different channel architectures is interpreted to have been governed by regional thrust‐tectonics, in combination with a high subsidence rate that promoted significant aggradation. In this scenario, the alternating ‘in sequence’ and ‘out of sequence’ tectonic pulses of the basin‐bounding thrusts controlled the activation of coarse‐clastic inputs in the basin and the resulting stacking architectures of channelized units. The tectonically‐driven confinement of the depositional system limited the lateral offset in channel stacking, preventing large‐scale avulsions. This study represents an excellent opportunity to analyze the stratigraphic evolution of a submarine channel system in tectonically‐active settings from an outcrop perspective. It should find wide applicability in analogous depositional systems, whose stratigraphic architecture has been influenced by tectonically‐controlled lateral confinement and associated lateral tilting.     

Damage assessment of churches after L’Aquila earthquake (2009)

L’Aquila earthquake, which occurred on April 6, 2009, proved the high vulnerability of cultural heritage, with particular reference to churches. Damage assessment in the emergency was carried out on more than 700 churches with a methodology aimed at recognizing the collapse mechanisms in the different architectonic elements of the church. The method was developed after the earthquake in Umbria and the Marches (1997) and has been widely used in the last decade; this approach is also very useful for seismic prevention, as it allows one to single out the most vulnerable structures. Some examples are presented in this paper, representative of recurrent damage in the main elements of the church: the fa?ade, the roof, the apse and the belfry. It emerges that, for a correct interpretation of damage and vulnerability, it is necessary a deep knowledge of local construction techniques and of the historic transformation sequence. Moreover, the bad behaviour of churches strengthened by modern techniques, such as the substitution of original timber roofs with stiff and heavy r.c. slabs, was observed. Starting from the observation of some case studies, the paper achieves some worth results, which may be useful for correctly driving future strengthening interventions.     

Physical and chemical control of the phytoplankton of Ahe lagoon, French Polynesia

The environmental characteristics of Ahe deep lagoon (Tuamotu Archipelago, French Polynesia) were studied over 3 years with the aim of explaining the spatial and temporal variability of the natural food available for pearl oysters with a special focus on phytoplankton biomass and global photosynthesis/respiration ratio of the lagoon. Chlorophyll averaged 0.34±0.01 μg L(-1) and our findings did not confirm increased phytoplankton biomass in deep lagoonal waters. Phytoplankton production appears to be limited firstly by nitrogen and respiratory processes overpass photosynthetic processes at least in the north-eastern edge of the atoll. Grazing by pearl oysters in culture seems to decrease the POC concentration but not the phytoplankton biomass. Oysters graze mainly on non chlorophyllian particles.     

Pearl oysters Pinctada margaritifera grazing on natural plankton in Ahe atoll lagoon (Tuamotu archipelago, French Polynesia)

In atoll lagoons of French Polynesia, growth and reproduction of pearl oysters are mainly driven by plankton concentration. However, the actual diet of black-lip pearl oysters Pinctada margaritifera in these lagoons is poorly known. To fill this gap, we used the flow through chamber method to measure clearance rates of P. margaritifera in Ahe atoll lagoon (Tuamotu Archipelago, French Polynesia). We found: (i) that pearl oysters cleared plankton at a rate that was positively related to plankton biovolume, (ii) that nanoflagellates were the main source of carbon for the pearl oysters, and (iii) that the quantity and origin of carbon filtrated by pearl oysters was highly dependent on the concentration and composition of plankton. These results provide essential elements for the comprehension of growth and reproduction variability of pearl oysters in atoll lagoons of French Polynesia.     

Survival and movements of Magellanic penguins rehabilitated from oil fouling along the coast of South America, 2000-2010

Oil pollution is a significant conservation concern. We examined data from six institutions along the coast of South America: Emergency Relief Team of the International Fund for Animal Welfare, Fundación Mundo Marino, Centro de Recupera??o de Animais Marinhos, Natura Patagonia, Associa??o R3 Animal, and Mar del Plata Aquarium and data from resightings in Argentina, Brazil, Chile and Falkland/Malvinas Islands. From 2000 to 2010, 2183 oiled Magellanic penguins were rehabilitated as part of the routine activities of these institutions or during emergency responses to eight oil spills in which they were involved; all rehabilitated penguins were flipper banded and released. Since their release, 41 penguins were resighted until 31 December 2011. The results demonstrate that, when combined with other prevention strategies, the rehabilitation of Magellanic penguins is a strategy that contributes to the mitigation of adverse effects of oil spills and chronic pollution to the species.     

Reconstruction of the volcanic history of the Tacámbaro-Puruarán area (Michoacán, México) reveals high frequency of Holocene monogenetic eruptions

The 690?km² Tacámbaro-Puruarán area located at the arc-front part of the Michoácan-Guanajuato volcanic field in the Trans-Mexican Volcanic Belt (TMVB) records a protracted history of volcanism that culminated with intense monogenetic activity in the Holocene. Geologic mapping, ⁴⁰Ar/³⁹Ar and ¹⁴C radiometric dating, and whole-rock chemical analyses of volcanic products provide insights to that history. Eocene volcanics (55–40?Ma) exposed at uplifted blocks are related to a magmatic arc that preceded the TMVB. Early TMVB products are represented by poorly exposed Pliocene silicic domes (5–2?Ma). Quaternary (<2?Ma) volcanoes (114 mapped) are mainly scoria cones with lavas (49 vol.%), viscous lava flows (22 vol.%), and lava shields (22 vol.%). Erupted products are dominantly either basaltic andesites (37 vol. %), or andesites (17 vol.%), or span across both compositions (28 vol.%). Basalts (9 vol.%), dacites (4 vol.%), shoshonites (2 vol.%), and other alkali-rich rocks (<3 vol.%) occur subordinately. Early-Pleistocene volcanism was bimodal (dacites and basalts) and voluminous while since 1?Ma small-volume eruptions of intermediate magmas have dominated. Higher rates of lithospheric extension in the Quaternary may have allowed a larger number of small, poorly evolved dikes to reach the surface during this period. Eruptive centers as old as 1.7?Ma are aligned in a NE direction parallel to both, basement faults and the direction of regional compressive stress, implying structural control on volcanic activity. Data suggest that volcanism was strongly pulsatory and fed by localized low-degree partial melting of mantle sources. In the Holocene, at least 13 eruptions occurred (average recurrence interval of 800?years). These produced ~3.8?km³ of basaltic andesitic to andesitic magma and included four eruptions dated at ~1,000; 4,000; 8,000; and 11,000?years bc (calibrated ¹⁴C ages). To date, this is one of the highest monogenetic eruption frequencies detected within such a small area in a subduction-related arc-setting. These anomalous rates of monogenetic activity in an area with thick crust (>30?km) may be related to high rates of magma production at depth and a favorable tectonic setting.     

40Ar–39Ar dating of volcanogenic products from the AND-2A core (ANDRILL Southern McMurdo Sound Project, Antarctica): correlations with the Erebus Volcanic Province and implications for the age model of the core

The AND-2A drillcore (Antarctic Drilling Program—ANDRILL) was successfully completed in late 2007 on the Antarctic continental margin (Southern McMurdo Sound, Ross Sea) with the aim of tracking ice proximal to shallow marine environmental fluctuations and to document the 20-Ma evolution of the Erebus Volcanic Province. Lava clasts and tephra layers from the AND-2A drillcore were investigated from a petrographic and stratigraphic point of view and analyzed by the ⁴⁰Ar–³⁹Ar laser technique in order to constrain the age model of the core and to gain information on the style and nature of sediment deposition in the Victoria Land Basin since Early Miocene. Ten out of 17 samples yielded statistically robust ⁴⁰Ar–³⁹Ar ages, indicating that the AND-2A drillcore recovered ≤230 m of Middle Miocene (∼128–358 m below sea floor, ∼11.5–16.0 Ma) and >780 m of Early Miocene (∼358–1093 m below sea floor, ∼16.0–20.1 Ma). Results also highlight a nearly continuous stratigraphic record from at least 358 m below sea floor down hole, characterized by a mean sedimentation rate of ∼19 cm/ka, possible oscillations of no more than a few hundreds of ka and a break within ∼17.5–18.1 Ma. Comparison with available data from volcanic deposits on land, suggests that volcanic rocks within the AND-2A core were supplied from the south, possibly with source areas closer to the drill site for the upper core levels, and from 358 m below sea floor down hole, with the “proto-Mount Morning” as the main source.     

Large-scale bedforms along a tideless outer shelf setting in the western Mediterranean

High-resolution multibeam swath-bathymetry and sediment samples were collected across the outer shelf region of the Columbretes Islands (southern Ebro continental shelf, western Mediterranean Sea). Bathymetric data from the submerged part of the Columbretes volcanic system revealed the presence of three main relict sand bodies along the outer shelf, at 80–116 m depth range, above which asymmetric and slightly asymmetric large and very large 2D and 3D subaqueous dunes were observed. These bed features were recognized, mapped and quantified with the aim of evaluating their potential formation mechanisms in relation to the local hydrodynamic and morphologic settings of the area. Dunes range from 150 to 760 m in wavelength and from tens of centimeters to 3 m in height, and are among the longest ever recognized in an outer shelf region. These bedforms are mostly composed of medium-sized sandy sediments, presumably coming from the degraded relict sand bodies on top of which they have developed, mixed with fine fractions from the recent draping holocenic sediments. The orientation of the dunes is SSW, progressively turning W towards the southernmost sector of the area, following the trend of the shelf-edge. Contemporary hydrodynamic measurements at the Ebro continental shelf-edge show that recorded currents are insufficient to form the observed bedforms and that stronger currents are required for sediment mobilization and dune formation. Based on their morphology and orientation, it is proposed that these bedforms are produced by the action of the southward-flowing Liguro-Provençal-Catalan (LPC) geostrophic current. The LPC probably reaches high near-bottom currents during energetic hydrodynamic events through interactions with the seafloor morphology of the study area. Subaqueous dunes are expected to be basically inactive features with respect to present-day processes, although they can be reactivated during high-energy events. The small Δh/λ ratio measured in the dune fields of the Columbretes shelf revealed that the dune heights fall below the values predicted by the Flemming (1988) global equation, as observed in other outer shelf settings also dominated by unidirectional flows. This may suggest a different morphodynamic character of large dunes formed on outer shelves in a micro-tidal regime.