Genetic diversity analysis with ISSR PCR on green algae Chlorella vulgaris and Chlorella pyrenoidosa

In the present study, genetic polymorphism and diversity in unicellular clones of Chlorella vulgaris Beijerinck and Chlorella pyrenoidosa Chick were studied with Inter Simple Sequence Repeats PCR (ISSR PCR). Samples including four clones of C. vulgaris and three clones of C. pyrenoidosa were purified by single-clone-choice method. For four C. vulgaris unicellular clones, the total number of the bands scored for 18 primers was 298; and the number of the polymorphic bands was 118, of which 39.6% were polymorphic. The size of PCR products ranged from 200 to 2 500 bp. The total number of bands scored for 18 primers, the number of polymorphic bands and the percentage of three C. pyrenoidosa unicellular clones was 194.83 and 30.8%, respectively. POPGENE analysis show that the average Nei genetic diversity (h *) and Shannon index of diversity (I *) in the four C. vulgaris unicellular clones was 0.2181 and 0.3208, respectively, which is slightly higher than those of the three C. pyrenoidosa unicellular clones (0.190 3 and 0.274 8), which agreed with the percentage of polymorphic bands in the mixed samples of the two species. The results suggest that ISSR is a useful method to Chlorella for intra-species genetic analysis.     

河流静水滩区潜流带水交换空间分布模式研究（英文）

Hyporheic zone(HZ) influences hydraulic and biogeochemical processes in and alongside streams, therefore, investigating the controlling geographic factors is beneficial for understanding the hydrological processes in HZ. Slack water pool (SWP) is an essential micro-topographic structure that has an impact on surface water and groundwater interactions in the HZ during and after high flows. However, only a few studies investigate HZ surface water and groundwater exchange in the SWP. This study used the thermal method to estimate the HZ water exchange in the SWP in a segment of the Weihe River in China during the winter season. The findings show that on the flow-direction parallel to the stream, river recharge dominates the HZ water exchange, while on the opposing flow-direction bank groundwater discharge dominates the water exchange. The water exchange in the opposing flow-direction bank is about 1.6 times of that in the flow-direction bank. The HZ water exchange is not only controlled by flow velocity but also the location and shape of the SWP. Great water exchange amount corresponds to the shape with more deformation. The maximum water exchange within the SWP is close to the river bank where the edge is relatively high. This study provides some guidelines for water resources management during flooding events.     

Effects of rainfall intensity and topography on rill development and rill characteristics on loessial hillslopes in China

Rill development is a major soil erosion process that causes severe soil degradation.This study examined the effects of representative rainfall intensities(50 and 75 mm h-1),slope gradients(10°and 150),and slope lengths(7.5 and 10.0 m)on rill development and rill characteristics on loessial hillslopes in China.Loessial soil was collected from the cropland of Ansai Town,Yan'an City,Shaanxi Province.The soil with 28.3%sand,58.1%silt,and13.6%clay was packed into a soil pan to conduct rainfall simulations in 2012.The results showed that the time of the knickpoint occurrence(5-16 min),the rill headcut extension(9-33 min),and the mean headward erosion rate(1.7-5.o cm min-1)were better representative indicators for reflecting the changes in the rill development than other indicators used in this study.For a quick evaluation of the rill erosion severity,the rill coverage ratio(1%-12%,generallyincreasing with an increase in the rainfall intensity)was better than the other indicators for treatments with different rainfall intensities,and the rill width-depth ratio(1.56-2.27,generally decreasing with an increase in the slope gradient)was better than the other indicators for treatments with different slope gradients.Furthermore,the rill inclination angle(8.2°-19.1°,significantly increasing with an increase in the slope length)and rill density(0.19-1.34 m·m-2,generally increasing with an increase in the slope length)were more suitable for evaluating the rill erosion severity on hillslopes with different slope lengths.Therefore,the representative indicators could reflect the differences in the rill development and rill characteristics under different rainfall and topographic situations.The study greatly improved the evaluation of rill erosion severity and the prediction of the development of rills for loessial hillslopes.     

铣削斜楔形区域形成及其对流场负压区的影响规律

【目的】研究在内喷淋冷却高速铣孔加工中,铣削斜楔形区域的形成对流场负压区的影响。【方法】对高速湿式铣削加工区域建立三维流场模型,应用计算流体力学软件Fluent,采用非结构化四面体网格单元,利用滑移网格技术对高速铣削过程进行三维瞬态计算。【结果】得到不同刃数、不同转速的立铣刀对同一孔径工件进行切削时流场的压力分布情况。三刃立铣刀斜楔形对流场负压影响最大,当立铣刀刃数增加时,此斜楔形区缩小,负压减小,转速为10 000～20 000 r/min时增长速度逐渐降低,20 000～35 000 r/min时增长速度逐渐增高。【结论】从流场角度分析,流场中最大负压值与转速呈正相关,转速的提高增加了抑制空化的难度;增加铣刀刃数,可减小流场斜楔形区面积,减小空化可能。     

生态地貌学研究体系及其功能提升探讨

地球表层是人类生存的家园,地表形态直接或间接影响人类生活、生产和社会经济活动。地貌学和生态学是与人类关系密切的学科。自然实体与人文实体都依附于地表,研究生态与地貌关系的科学便统称为生态地貌学,直接影响人类生存选址、生存保障、生产方向、产业布局、交通、城乡建设等,因此,生态地貌学是保障人类生存与社会经济可持续协调发展的基础性与应用性极强的学科,是国家实现生态文明战略的基础性学科。生态地貌学研究生态与地貌两者相互作用形成的生态地貌综合实体,学科体系包含地貌基础学科、生态基础学科及其相互作用形成的生态地貌学科。生态地貌学科下又包含生态地貌区划学、生态地貌类型学、生态地貌资源学、生态地貌岩态学、生态地貌遥感与GIS技术、生态地貌管理与规划等分支学科,是目前仍然属于探索性的学科。生态地貌结构可表达为：由地貌与生物成分、类型、区域组合、数量构成及其空间排列组合方式。从生态地貌结构理论出发,对其功能进行系统梳理,主要可以归纳为区域结构、类型结构、资源结构、岩态结构等多种类型。不同结构类型具有不同的功能,主要功能有提升中国自然地理区划质量与空间定位功能、生态评估与生态设计功能、土地利用评估与利用结构调整功能、地质地貌灾害成因和防灾减灾对策功能等。故该研究意在实现生态地貌功能间协调、高效可持续,通过对各功能进行整合,形成功能体系,并从调控管理上提出了提升功能能力的设计路径。     

1961—1987年与1988—2014年辽宁省气候区划界线变化及可能气候成因

基于同一区划方法、指标体系,使用1961—2014年辽宁省52站气象观测资料,分析辽宁省气温、气候区划指标、范围及界线的变动特征。结果表明：辽宁省年均气温在1988年发生一次突变,突变后气温开始显著上升;≥10 ℃积温日数比较显著地响应气温突变,而干燥指数、7月平均气温变化不显著。在空间分布上区划指标值均存在不同程度的变化。① 全省≥10 ℃积温日数均出现增加,但在中西部地区显著增加;② 在盘锦-抚顺一线以北（南）,气候总体呈不显著变湿（干）趋势;③ 7月平均气温呈缓慢上升趋势。区划范围及界线位置出现更加显著地变化：① 暖温带范围主要向北向东扩展,中温带向东收缩;② 半湿润区范围主要向北向西扩展,半干旱区向西北方向收缩,湿润区范围基本不变;③ Tb范围显著向北向东扩展,Ta范围向北向东收缩。在此基础上分析了气候格局变化的可能气候成因,发现突变后≥10 ℃积温日数期间500 hPa高度场增加与4月和10月东亚冬季风减弱,4—10月东北冷涡持续天数增加和7月500 hPa高度场增加,可能分别是温度带,Tb区、Ta区和半湿润区、半干旱区变化的原因。     

Gaussian decomposition and component pigment spectral analysis of phytoplankton absorption spectra

The absorption spectrum of phytoplankton is an important bio-optical parameter for ocean color hyperspectral remote sensing;its magnitude and shape can be affected considerably by pigment composition and concentration.We conducted Gaussian decomposition to the absorption spectra of phytoplankton pigment and studied the spectral components of the phytoplankton,in which the package effect was investigated using pigment concentration data and phytoplankton absorption spectra.The decomposition results were compared with the corresponding concentrations of the five main pigment groups(chlorophylls a,b,and c,photo-synthetic carotenoids(PSC),and photo-protective carotenoids(PPC)).The results indicate that the majority of residual errors in the Gaussian decomposition are0.001 m~(-1),and R~2 of the power regression between characteristic bands and HPLC pigment concentrations(except for chlorophyll b)was0.65 or greater for surface water samples at autumn cruise.In addition,we determined a strong predictive capability for chlorophylls a,c,PPC,and PSC.We also tested the estimation of pigment concentrations from the empirical specific absorption coefficient of pigment composition.The empirical decomposition showed that the Ficek model was the closest to the original spectra with the smallest residual errors.The pigment decomposition results and HPLC measurements of pigment concentration are in a high consistency as the scatter plots are distributed largely near the 1:1 line in spite of prominent seasonal variations.The Wozniak model showed a better fit than the Ficek model for Chla,and the median relative error was small.The pigment component information estimated from the phytoplankton absorption spectra can help better remote sensing of hyperspectral ocean color that related to the changes in phytoplankton communities and varieties.     

Farmer-based Rice Cropping Systems in the Poyang Lake Region,China: Analysis of Characteristics and Policy Implications

This paper examines the spatial characteristics of farmer/household behaviors in regional rice cropping systems (RCS), and the results provide necessary information for developing strategies that will maintain regional food security. Through field study and statistical analysis based on 402 households questionnaires finished in 2014-2015 in the Poyang Lake Region (PLR) of China, we arrived at two main conclusions. First, single- and double cropping rice were found across the study area, but showed a general distribution trend, with double cropping rice in the southeast part (especially in Jinxian county) and single cropping rice in the northwest (particularly in De’an county). Second, the household decisions concerning RCS varied in different parts of the PLR, but double cropping was the dominant type, with about 63.57% of the respondent households in the PLR cultivating double cropping rice. However, the multiple-cropping index of paddy rice was only 1.55. About 3% of interviewed households had altered their RCS during this period. Based on these findings, the local governments should guide farmers’ paddy field cultivation behaviors by increasing the comparative efficiency of rice production, promoting appropriate scale operations and land conversion, as well as optimizing rice growing conditions to improve the multiple cropping index and enhance food provision. Finally, land-use efficiency and more sustainable use of land resources should be improved.     

一例异育银鲫(Carassius auratus gibelio)暴发性出血病病原分析

2016年6月,江苏某异育银鲫(Carassius auratus gibelio)养殖场暴发一种传染性急性出血病,造成养殖银鲫大量死亡。为分析此次疾病病因及流行规律,本研究从发病养殖场采集患出血病的异育银鲫,从细菌、病毒及寄生虫三个方面对病原进行了分析。采用病原菌分离、组织病理学观察、超薄切片电镜观察、病毒核酸分析、回感实验等对病原进行鉴定。结果显示从发病鲫鱼体内分离到病毒一株,未发现寄生虫及细菌感染。经测序及序列分析,该病毒为鲤疱疹病毒Ⅱ型(Cyprinid herpesvirus2,CyHV-2)病毒,组织病理学观察结果显示患病鱼的鳃和肾脏有明显病变,电镜下可观察到病鱼脾脏组织有带囊膜的球形病毒,囊膜直径约为170—200nm,病毒衣壳直径约为110—120nm,核心直径约为60nm,用组织匀浆感染鲫鱼囊胚细胞系(CGB)可稳定地观察到典型的细胞病变,用患病鱼组织匀浆液人工感染异育银鲫的死亡率高达100%,荧光定量PCR检测到该病毒可感染多器官,其中以脾脏中病毒含量最高,其次是脑,肝脏中最少。本研究可为CyHV-2的诊断防控及疫苗研制提供资料。     

氨氮急性攻毒对水产经济动物棘胸蛙(Paa spinosa)蝌蚪死亡率、排氨率、耗氧率及窒息点的影响

以体长(1.932±0.204)cm、体质量(1.386±0.055)g的棘胸蛙蝌蚪为实验动物,在水温(24±0.2)℃、DO(7.30±0.01)mg/L、pH 7.30±0.01条件下,采用静水停食法开展了氨氮对蝌蚪的急性攻毒实验,并以此为基础,测定了不同氨氮质量浓度胁迫下棘胸蛙蝌蚪的排氨率、耗氧率、氧氮比及窒息点。结果表明:(1)蝌蚪对氨氮急性攻毒具明显的运动避毒行为,濒死个体的背部皮肤和肝脏均具明显的色变症状;(2)氨氮对蝌蚪的急性致死率具明显的剂量—时间效应,24h、48h、72h、96h LC50依次为177.5、151.7、148.6和146.8mg/L,毒性时段蓄积程度系数呈持续下降趋势,24—48h、48—72h、72—96h的时段MAC值分别为84.04%、10.1%和5.86%;(3)蝌蚪夜均、昼均、日均及时段排氨率随氨氮质量浓度增加均呈阶梯式下降趋势,其中时段排氨率与对照组均无显著差异的为9.80mg/L实验组,夜均、昼均、日均及时段排氨率与对照组均无显著差异的仅为2.45mg/L实验组(P0.05);(4)氨氮对蝌蚪呼吸耗氧具低毒兴奋效应,其夜均、昼均和日均耗氧率随氨氮质量浓度增加均呈先升后降趋势,峰值氨氮质量浓度范围均为4.90—7.35mg/L(P0.05),与对照组均无显著差异的仅为14.70mg/L实验组(P0.05),窒息点含氧量随氨氮质量浓度增加呈先降后升趋势,谷底出现于4.90mg/L实验组(P0.05),与对照组无显著差异的仅为9.80mg/L实验组(P0.05);(5)实验期间,蝌蚪氧氮比(O:N)波动于18.87—25.34之间,昼均和日均氧氮比(O:N)随氨氮质量浓度增加均依次呈"上升—稳定—下降—再稳定"之趋势,两者的峰值氨氮质量浓度范围分别为4.90—14.70mg/L和4.90—9.80mg/L,夜均氧氮比(O:N)呈先升后降趋势,峰值出现于7.35mg/L实验组(P0.05)。