The role of water mass dynamics in controlling bacterial abundance and production in the middle Adriatic Sea

Month-to-month fluctuations in the abundance of bacteria and heterotrophic nanoflagellates (HNF) and bacterial production, as well as various chemical (nutrients, oxygen) and physical (salinity, temperature) parameters were analysed at a station located in the open middle Adriatic Sea during one decade (1997-2006). Being influenced by both coastal waters and open Adriatic circulation in the surface layer, and by the deep Adriatic water masses in the deep layers (100 m), this station is quite suitable for detecting the environmental changes occurring in the open Adriatic Sea with respect to the circulation of its water masses and their long-term changes and anomalies. Multivariate methods were used to identify seasonal and inter-annual changes of the investigated parameters, associating observed changes to the changes in Adriatic water masses and circulation regimes. The analyses showed that bacterial abundance and production were controlled by different water mass dynamics during 1997-2001 compared to 2002-2006 period, particularly noticeable in different seasonal patterns of biological parameters. The interplay between North Adriatic Dense Water (NAdDW) and Levantine Intermediate Water (LIW) resulted in a change in the available nutrients (NAdDW is poor in orthophosphates), and as a consequence different bacterial abundance and production. A few periods were examined in detail, such as 2004, when LIW inflow was particularly strong and was accompanied by an increase of bacterial and HNF abundances, as well as of bacterial production.     

The influence of 70 and 120 kHz tonal signals on the behavior of harbor porpoises (Phocoena phocoena) in a floating pen

Two harbor porpoises in a floating pen were subjected to five pure tone underwater signals of 70 or 120kHz with different signal durations, amplitudes and duty cycles (% of time sound is produced). Some signals were continuous, others were intermittent (duty cycles varied between 8% and 100%). The effect of each signal was judged by comparing the animals' surfacing locations and number of surfacings (i.e. number of respirations) during test periods with those during baseline periods. In all cases, both porpoises moved away from the sound source, but the effect of the signals on respiration rates was negligible. Pulsed 70kHz signals with a source level (SL) of 137dB had a similar effect as a continuous 70kHz signal with an SL of 148dB (re 1muPa, rms). Also, a pulsed 70kHz signal with an SL of 147dB had a much stronger deterring effect than a continuous 70kHz signal with a similar SL. For pulsed 70kHz signals (2s pulse duration, 4s pulse interval, SL 147dB re 1muPa, rms), the avoidance threshold sound pressure level (SPL), in the context of the present study, was estimated to be around 130dB (re 1muPa, rms) for porpoise 064 and around 124dB (re 1muPa, rms) for porpoise 047. This study shows that ultrasonic pingers (70kHz) can deter harbor porpoises. Such ultrasonic pingers have the advantage that they do not have a "dinner bell" effect on pinnipeds, and probably have no, or less, effect on other marine fauna, which are often sensitive to low frequency sounds.     

Anoxia over the western continental shelf of India: bacterial indications of intrinsic nitrification feeding denitrification

Studies on the Arabian Sea coastal anoxia have been of immense interest, but despite its ecological significance there is sparse understanding of the microbes involved. Hence, observations were carried out off Goa (15 degrees 30'N, 72 degrees 40'E to 15 degrees 30'N, 72 degrees 59'E) to understand the processes that mediate the changes in various inorganic nitrogen species in the water column during anoxia. Water column chemistry showed a clear distinct oxic environment in the month of April and anoxic condition in October. Our study based on microbial signatures indicated that oxygen deficit appeared as a well-defined nucleus almost 40 km away from the coast during the oxic period (April) and spreads there after to the entire water column synchronizing with the water chemistry. Striking results of net changes in inorganic nitrogen species in nitrification blocked and unblocked experimental systems show that denitrification is the predominant process in the water column consuming available nitrate ( approximately 0.5 microM) to near zero levels within approximately 72 h of incubation. These observations have been supported by concomitant increase in nitrite concentration ( approximately 4 microM). Similar studies on denitrification-blocked incubations, demonstrate the potential of nitrification to feed denitrification. Nitrification could contribute almost 4.5 microM to the total nitrate pool. It was found that the relation between ammonium and total dissolved inorganic nitrogen (DIN) pool (r=0.98, p<0.001, n=122) was significant compared to the latter with nitrite and nitrate. The occurrence of high ammonium under low phosphate conditions corroborates our observations that ammonium does not appear to be locked under low oxygen regimes. It is suggested that ammonium actively produced by detrital breakdown (ammonification) is efficiently consumed through nitrification process. The three processes in concert viz. ammonification, nitrification and denitrification appear to operate in more temporal and spatial proximity than hitherto appreciated in these systems and this gives additional cues on the absence of measurable nitrate at surface waters, which was earlier attributed only to efficient algal uptake. Hence we hypothesize that the alarming nitrous oxide input into the atmosphere could be due to high productivity driven tighter nitrification-denitrification coupling, rather than denitrification driven by extraneous nitrate.     

“Trends” and variations of global oceanic evaporation data sets from remote sensing

The variability in global oceanic evaporation data sets was examined for the period 1988-2000. These data sets are satellite estimates based on bulk aerodynamic formulations and include the NASA/Goddard Space Flight Center Satellite-based Surface Turbulent Flux version 2 （ GSSTF2）, the Japanese-ocean flux using remote sensing observations （J-OFURO）, and the Hamburg Ocean-Atmosphere Parameters and Fluxes from Satellite version 2 （HOAPS2）. The National Center for Environmental Prediction （NCEP） reanalysis is also included for comparison. An increase in global average surface latent heat flux （SLHF） can be observed in all the data sets. Empirical mode decomposition （EMD） shows long-term increases that started around 1990 for all remote sensing data sets. The effect of Mt. Pinatubo eruption in 1991 is clearly evident in HOAPS2 but is independent of the longterm increase. Linear regression analyses show increases of 9.4%, 13.0%, 7. 3%, and 3.9% for GSSTF2, J-OFURO, HOAPS2 and NCEP, for the periods of the data sets. Empirical orthogonal function （EOF） analyses show that the pattern of the first EOF of all data sets is consistent with a decadal variation associated with the enhancement of the tropical Hadley circulation, which is supported by other satellite observations. The second EOF of all four data sets is an ENSO mode, and the correlations between their time series and an SO1 are 0.74, 0.71,0.59, and 0.61 for GSSTF2, J-OFURO, HOAPS2, and NCEP in that order. When the Hadley modes are removed from the remote sensing data, the residue global increases are reduced to 2.2% , 7. 3%, and 〈 1% for GSSTF2, J-OFURO and HOAPS, respectively. If the ENSO mode is used as a calibration standard for the data sets, the Hadley mode is at least comparable to, if not larger than, the ENSO mode during our study period.     

Validation of Foreground Emission Model using GALEX Medium Observations

The study of diffuse ultraviolet(UV) background radiation is vital in the investigation of stellar and galactic evolution. Space-based UV observations are comprised of both foreground and background radiations. The foreground emission in an observation is a result of solar contamination in the direction of observation. In our previous work, we modeled airglow(one of the major constituents of the foreground emission) as a function of10.7 cm Solar Flux and Sun Angle with great accuracy using GALEX...     

A Statistical Study of Magnetic Flux Emergence in Solar Active Regions Prior to Strongest Flares

Using the data on magnetic field maps and continuum intensity for Solar Cycles 23 and 24,we explored 100 active regions (ARs) that produced M5.0 or stronger flares.We focus on the presence/absence of the emergence of magnetic flux in these ARs 2–3 days before the strong flare onset.We found that 29 ARs in the sample emerged monotonically amidst quiet-Sun.A major emergence of a new magnetic flux within a pre-existing AR yielding the formation of a complex flare-productive configuration was observ...     

Effects of cadmium, zinc and nitrogen status on non-protein thiols in the macroalgae Enteromorpha spp. from the Scheldt Estuary (SW Netherlands, Belgium) and Thermaikos Gulf (N Aegean Sea, Greece)

Enteromorpha prolifera (Scheldt Estuary) and E. linza (Thermaikos Gulf) were incubated at three salinities with 100 and 200microgL(-1)Cd and Zn. The objective was to measure effects of Cd, Zn and nitrogen (N) status on the pools of metal-binding non-protein thiols: glutathione and phytochelatins, (gamma-glutamyl-cysteinyl)(n)-glycine (PC). In E. linza, ammonium pools were higher, but amino acid pools, total N and protein contents were lower than in E. prolifera. Reduced glutathione (GSH) pools were positively correlated with free glutamate and protein contents. In E. linza GSH pools increased and the ratio of reduced to oxidized glutathione (GSH:(GSH+0.5GSSG)), an indicator of oxidative stress, decreased with Cd contents, indicating Cd-induced glutathione oxidation. Total glutathione pools (reduced plus oxidized) ranged from 16nmolSgdwt(-1) in controls (at 0.5micromolCdgdwt(-1)) to 179nmolSgdwt(-1) (at 1.9micromolCdgdwt(-1)) at the highest cadmium dosage. Cadmium stimulated PC synthesis in E. prolifera which suggests that in N-rich algae, glutathione pools were high enough for PC synthesis. In both species GSH and protein increased with Zn contents, whereas GSH:(GSH+0.5GSSG) decreased, which would indicate Zn-induced oxidative stress; in E. linza, at the highest salinity the glutathione redox ratio decreased from 0.61 (at 2.9micromolZngdwt(-1)) to 0.26 (at 4.9nmolSgdwt(-1)) (at 0.5molCdgdwt(-1)). PCs were not synthesized in response to Zn, which may have resulted in Zn-induced GSH oxidation. The presence of both oxidative effects (Cd, Zn) and detoxification (Cd) could be identified by observing the responses of glutathione and PC pools to metal stress.     

渤海海域浮游病毒和浮游细菌的丰度变化与环境因子之间的耦联关系

Uncovering the role of environmental factors and finding critical factors which harbor significant fractions in governing microbial communities remain key questions in coastal marine systems. To detect the interactions between environmental factors and distributions of virio-and bacterioplankton in trophic coastal areas, we used flow cytometry to investigate the abundance of virio-and bacterioplankton covering 31 stations in the Bohai Sea of China. Our results suggested that the average abundance of total virus(TV) in winter(～2.29×10~8 particles/mL)was slightly lower than in summer(～3.83×10~8 particles/mL). The mean total bacterial abundance(TB) was much lower in winter(～2.54×107 particles/mL) than in summer(～5.43×10~7 particles/mL). Correlation analysis via redundancy analysis(RDA) and network analysis among virioplankton, bacterioplankton and environmental factors revealed that the abundances of viral and bacterial subpopulations depend on environmental factors. In winter, only temperature significantly influenced the abundances of virio-and bacterioplankton. In summer, in addition to temperature, both salinity and nutrient(SiO_2) had a remarkable impact on the distribution of virioand bacterioplankton. Our results showed a clear seasonal and trophic pattern throughout the whole water system, which revealed that temperature and eutrophication may play crucial roles in microbial distribution pattern.     

渤海东部潮流动力地貌特征

渤海东部发育了典型的潮流动力地貌体系，它由老铁山水道冲刷槽、辽东浅滩沙脊和渤中浅滩沙席三者组成。前者为潮流侵蚀区，后两者为潮流沉积区。老铁山水道表层最大流速可达５ｋｎ，潮流强烈侵蚀海底；辽东浅滩表层最大流速１．３￣２．３ｋｎ，Ｍ２分潮椭率绝对值小于０．４，潮流往复强，形成潮流沙脊；渤中浅滩表层最大流速为１．２￣１．６ｋｎ，Ｍ２分分潮椭率绝对值大于０．４，潮流旋转性强，形成潮流沙席。６号沙脊南端详测