Identification of five sea cucumber species through PCR-RFLP analysis

Sea cucumbers are traditional marine food and Chinese medicine in Asia. The rapid expansion of sea cucumber market has resulted in various problems, such as commercial fraud and mislabeling. Conventionally, sea cucumber species could be distinguished by their morphological and anatomical characteristics; however, their identification becomes difficult when they are processed. The aim of this study was to develop a new convenient method of identifying and distinguishing sea cucumber species. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of mitochondrial cytochrome oxidase I gene (COI) was used to identifing five sea cucumber species (Apostichopus japonicus, Cucumaria frondosa, Thelenota ananas, Parastichopus californicus and Actinopyga lecanora). A 692 bp fragment of COI was searched for BamHI, KpnI, PstI, XbaI and Eco31I restriction sites with DNAMAN 6.0, which were then used to PCR-RFLP analysis. These five sea cucumber species can be discriminated from mixed sea cucumbers. The developed PCR-RFLP assay will facilitate the identification of sea cucumbers, making their source tracing and quality controlling feasible.     

湖南两次流域性致洪暴雨异同点分析

利用常规气象资料、水情资料及灾情资料,对发生地点、季节及影响系统相似的2010年7月8-14日和2012年7月11-19日两次湖南省持续性大范围暴雨天气过程进行对比分析,结果表明：（1）两次暴雨过程均具有持续时间长、暴雨频繁且集中、累计雨量大、洪涝严重等特点;（2）东北冷涡加强、副高东退南落,冷、暖空气在湖南交汇,导致连续性暴雨发生;（3）“10.7”暴雨过程冷、暖气流势力相当,雨带呈东西向,稳定少动,致灾性强.而“12.7”暴雨过程冷空气势力较强,雨带呈移动性,虽然致灾范围广,但灾情较前者轻.     

QuikSCAT和ASCAT卫星反演风场在中国南海北部的适用性研究

对QuikSCAT和ASCAT原始轨道10 m反演风场与浮标资料在中国南海北部的统计检验分析结果表明:两套卫星资料在中国南海北部具有较好的适用性,QuickSCAT反演风速偏高0.46 m·s~(-1),ASCAT反演风速在近海偏高0.45 m·s~(-1),在开阔海域偏高0.07 m·s~(-1)。超过半数的QuickSCAT反演风向误差30°。在近岸海域,ASCAT反演风向误差30°的超过56%,在开阔海域,误差绝对值30°的达到64%。小风时卫星反演风速偏大,大风时卫星反演风速明显偏小,且白天的偏差大于夜间;在5~10 m·s~(-1)风速条件下,两者的一致性较好。用WRF模式模拟的近海风能资源存在高估的可能,卫星资料对近海风能资源评估是个有益的补充,本文对卫星反演风场误差的分析结果也可以为卫星反演风场的资料同化提供参考。     

地面沉降对高速铁路的影响分析

北京平原区快速发展的地面沉降对高速铁路的发展构成了威胁,地面沉降与过量开采地下水造成的水位下降关系密切,为此有针对性地开展基于高速铁路的地下水动态与地面沉降相关关系研究对于高铁安全运行意义重大,特别是对于制定高铁沿线地下水开采方案、地面沉降减缓措施和工程措施至关重要。基于其对高速铁路的影响模式,本文将地面沉降分为区域沉降和局部沉降两种类型。针对区域沉降,利用Logistic方程,使用天竺、望京及王四营分层地面沉降和地下水位数据,构建了不同层位地下水水位变化与地面沉降之间的相关关系模型,通过ABAQUS计算局部地区,对于6m高路堤和15m CFG桩处理深度的地基而言,当渗透系数k=2m／d,距离线路边缘25m处浅层地下水下降10m将产生约61—85mm的沉降。     

内蒙古敖汉旗克力代岩体锆石U-Pb年代及地球化学

通过LA-ICP-MS锆石U-Pb测年对内蒙古敖汉旗克力代岩体进行研究。结果表明,岩浆锆石的加权平均年龄为263±1 Ma,表明其结晶年龄为中二叠世。岩石地球化学分析表明,岩体具有高Si(SiO2=69.94%～72.56%),富ALK(Na2O+K2O=8.04%～9.23%),贫Fe(FeOT=1.50%～1.82%)、Mg(MgO=0.65%～0.86%)、Ti(TiO2=0.32%～0.35%)的特点;A/CNK值为0.87～0.93,为准铝质;A/NKC1.1,显示出I型花岗岩特征。固结指数(SI)为6.06～7.36,分异指数(DI)为88.82～91.82,说明岩体经历了较强的分异演化作用。稀土元素总量较低(ΣREE=91.76×10-6～143.16×10-6),轻稀土明显富集,重稀土相对亏损,LREE/HREE值平均为9.14,(La/Yb)N平均值为8.36,δEu平均值为0.58,为Eu亏损型。大离子亲石元素(LILE)Rb、K较富集,强烈亏损高场强元素(HFSE)Nb、Ti、Ta。因此,判定克力代岩体为高钾钙碱性I型花岗岩。结合测年结果和地球化学特征,判定该岩体为晚海西期华北板块和西伯利亚板块碰撞作用形成的同碰撞型花岗岩。     

广西田林县八渡平乐金矿找矿远景

平乐金矿床位于桂西北-黔西南-滇东南金锑银多金属成矿区,与其东侧典型金矿床高龙金矿成矿地质背景有一定的类似性。本次工作通过调查取样,结合高龙金矿地质特征及控矿因素对比分析,对该矿床的成矿远景进行了初步分析。     

矿井涌水量预测的几种常用方法对比——以宁夏宁东煤田鸳鸯湖矿区麦垛山煤矿11采区水文地质补勘为例

我国许多煤矿区,由于地下水频繁突入,造成淹井或因突水威胁使大量煤炭资源难以开发。因此,准确预测矿井涌水量,为矿井防治水提供依据,对煤矿床安全开采和长远发展至关重要。以宁东煤炭基地麦垛山煤矿为例,采用解析法、水文地质比拟法和三维渗流数值法等六种预测方法,对该煤矿11采区110203、110208工作面2#煤顶板直罗组底部砂岩含水层进行了涌水量预测。通过各种方法的分析比较,最后推荐采用最小二乘比拟法预算结果做最终矿井涌水量。     

Geochemistry,and zircon U–Pb and molybdenite Re–Os geochronology of Jilongshan Cu–Au deposit,southeastern Hubei Province,China

The Jilongshan skarn Cu–Au deposit is located at the Jiurui ore cluster region in the southwestern part of the Middle–Lower Yangtze River valley metallogenic belt. The region is characterized by NW‐, NNW‐ and EW‐trending faults and the mineralization occurs at the contact of lower Triassic carbonate rocks and Jurassic granodiorite porphyry intrusions. The intrusives are characterized by SiO₂, K₂O, and Na₂O concentrations ranging from 61.66 to 67.8 wt.%, 3.29 to 5.65 wt.%, and 2.83 to 3.9 wt.%, respectively. Their A/CNK (A/CNK = n(Al₂O₃)/[n(CaO) + n(Na₂O) + n(K₂O)]) ratio, δEu, and δCe vary from 0.77 to 1.17, 0.86 to 1, and 0.88 to 0.96, respectively. The rocks show enrichment in light rare earth elements ((La/Yb)_N = 7.61–12.94) and large ion lithophile elements (LILE), and depletion in high field strength elements (HFSE), such as Zr, Ti. They also display a peraluminous, high‐K calc‐alkaline signature typical of intrusives associated with skarn and porphyry Cu–Au–Mo polymetallic deposits. Laser ablation inductively coupled plasma spectrometry (LA‐ICP‐MS) zircon U–Pb age indicates that the granodiorite porphyry formed at 151.75 ± 0.70 Ma. A few inherited zircons with older ages (677 ± 10 Ma, 848 ± 11 Ma, 2645 ± 38 Ma, and 3411 ± 36 Ma) suggest the existence of an Archaean basement beneath the Middle–Lower Yangtze River region. The temperature of crystallization of the porphyry estimated from zircon thermometer ranges from 744.3 °C to 751.5 °C, and 634.04 °C to 823.8 °C. Molybdenite Re–Os dating shows that the Jilongshan deposit formed at 150.79 ± 0.82 Ma. The metallogeny and magmatism are correlated to mantle–crust interaction, associated with the subduction of the Pacific Plate from the east. Copyright © 2013 John Wiley & Sons, Ltd.     

北冈底斯带日土县-拉梅拉山口花岗岩体的岩石地球化学特征、锆石U-Pb测年及Hf同位素组成

对西藏西部日土县城以南-拉梅拉山口一带的花岗岩体开展了详细的岩相学、岩石地球化学和锆石U-Pb年代学及Hf同位素研究。所有样品铝饱和指数A/CNK集中在0.76~1.0之间,为准铝质类型。CIPW标准矿物组合为Q+Or+Ab+An+Di（或C）+Hy。在稀土元素配分图中呈现出右倾缓倾斜型的特征,轻稀土元素富集并出现较强的分馏作用,重稀土元素无分馏-轻微分馏。δEu在0.56~0.99范围之间,属于铕亏损型。大离子亲石元素出现分化,富集Rb、Pb、Th而亏损K、Ba,高场强元素Nb、Ta、Ti等明显亏损。获得钾长花岗岩、二长花岗岩及花岗闪长岩中岩浆结晶锆石的LA-ICP-MS U-Pb年龄分别为：79.4±0.4Ma、 81.0±0.5Ma和81.3±0.5Ma,结合锆石稀土元素和岩浆振荡环带特征及Th/U比值,上述年龄结果可代表岩石的结晶年龄,表明该套岩体为晚白垩世侵位的大型岩基。两件样品的锆石均具有正的Hf同位素初始比值ε_Hf（t）,两阶段Hf模式年龄（t_DM2）分别介于547.5~658.0Ma、523.4~710.2Ma之间。分析认为该套岩体的物质来源应该为富角闪石的下地壳,可能为幔源岩浆首先侵入到地壳基底岩石中形成新生地壳,然后在温度约为700~800℃之间、压力<8kbar且富含流体的影响下,这种既有新生地壳又有古老基底地壳构成的混合地壳发生部分熔融而形成。这一结论与野外宏观露头上岩体中大量发育暗色微粒包体等直接岩石学证据相佐证。结合区域构造演化及岩体所处的大地构造位置,该套花岗岩体应该形成于洋壳闭合时的碰撞造山过程,其形成与侵位与北侧班公湖-怒江结合带的构造演化有成因上的联系,是班公湖-怒江特提斯洋向南的俯冲碰撞的产物。     

排桩内支撑基坑典型失稳原因及处理对策分析

排桩内支撑支护结构是软土地区建筑工程中常用的一种基坑支护结构型式。从设计和施工2方面对影响排桩内支撑支护结构稳定的因素进行了分析,并结合某基坑工程破坏实例,对排桩内支撑支护结构典型失稳原因进行了分析探讨,提出了处理对策。开挖结果表明处理措施安全有效。