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241.
Green-lipped mussels (Perna viridis) were exposed to Aroclor 1254 (0.5, 5 and 50 microgl(-1)) and a mixture of benzo[a]pyrene (B[a]P) and Aroclor 1254 (0.3+0.5 and 3+5 microgl(-1)) for 12 days. On day 0, 1, 3, 6 and 12, the levels of DNA strand breaks in the mussel hepatopancreas were monitored using an alkaline unwinding assay. The results were compared to the findings of a previous study in which the levels of DNA strand breakage in the same species were measured following exposure to various concentrations of B[a]P (0.3, 3 and 30 microgl(-1)). The results indicated that Aroclor 1254 at a concentration =50 microgl(-1) did not cause any increase in DNA strand breaks while B[a]P at concentrations ranging from 0.3 to 3 microgl(-1) caused an increase in strand breaks after one day of exposure, followed by an apparent rapid recovery. In contrast, exposure to 30 microgl(-1) B[a]P caused no increase in DNA strand breaks over the exposure period. This was postulated to be due to an early elicitation of the DNA repair system by the relatively high exposure level of B[a]P. This hypothesis was tested in the present study, and the results suggest that exposure to the high B[a]P concentration might have elicited the defense mechanism within the mussels, resulting in no observed increase in DNA strand breaks. An increase in strand breaks was, however, evident when the mussels were exposed to lower B[a]P levels. The levels of DNA strand breaks were correlated with the body burden of B[a]P and Aroclor 1254 but no significant relationship was observed, possibly owing to the rapid metabolism of the toxicant and/or an effective DNA repair mechanism. As a result, DNA strand breakage in the hepatopancreas of green-lipped mussels may not be a suitable biomarker of exposure to the above toxicants in the marine environment. Our findings also suggest that it would be instructive to investigate (1) the role of DNA repair enzymes in the exposed mussels; and (2) the correlation between the activity of these enzymes and the body burden of specific toxicants. 相似文献
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对于地震物理学和断裂运动研究而言,探索断裂带构造和地震行为之间的联系是至关重要的。地震代表滑动行为的一个极端;摩擦熔融和假玄武玻璃为动力破裂过程中的局部滑动提供了野外证据[1-3]。无震滑动则 相似文献
244.
Cross-ecosystem subsidies, such as terrestrial invertebrates and leaf litter falling into water as resources for aquatic communities,
can vary across environmental gradients. We examined whether the effect of terrestrial subsidy inputs on benthic invertebrates
was mediated by resident coastal cutthroat trout (Oncorhynchus clarki) in two representative streams. We experimentally manipulated the input rates (reduced, ambient) of terrestrial subsidies
(terrestrial invertebrates and leaf litter) as well as the presence or absence of cutthroat trout in the two streams. The
hypothesis that the reduction of terrestrial subsidies to the stream influences benthic invertebrate assemblages was supported
by experimental results. The treatments of terrestrial subsidy reduction and cutthroat trout presence had a significant negative
effect on benthic invertebrate community biomass and shredder biomass in East Creek with high natural terrestrial subsidy
input and small amount of large wood in channel. In contrast, results from Spring Creek with low subsidy input and large amount
of large wood in channel showed that only the terrestrial subsidy reduction significantly reduced the biomass of shredders.
The effects of the terrestrial subsidy input and trout predation on benthic invertebrate communities varied between the two
streams. Our results indicate that a subsidy effect on benthic communities can vary between nearby streams differing in canopy
and habitats. This study, with the major finding of highly context-dependent effects of spatial subsidies, suggests that the
interplay of resource subsidies and predators on invertebrate community assemblages can be site-specific and context-dependent
on habitat features. 相似文献
245.
Non-stationary Generation of Weak Turbulence for Very Stable and Weak-Wind Conditions 总被引:4,自引:4,他引:0
Larry Mahrt Christoph Thomas Scott Richardson Nelson Seaman David Stauffer Matthias Zeeman 《Boundary-Layer Meteorology》2013,147(2):179-199
Turbulence measurements for very stable conditions near the surface are contrasted among three sites: a high altitude basin during winter with grass or snow-covered grass, a broad valley with complex agricultural land use, and a more narrow valley that is influenced by a valley cold pool and cold air drainage. In contrast to previous studies, this investigation emphasizes the very weak turbulence with large bulk Richardson number occurring during extensive periods between brief mixing events. The relationship of the turbulence to the non-stationary wind and stratification is examined along with the impact of short-term flow accelerations, directional shear and downward diffusion of turbulence from higher levels. The failure of the turbulence for strong stratification to decrease with further increase of stratification is explored. Additional analyses are applied to weak-wind cases for the entire range of stratification, including weak stratification associated with cloudy conditions. 相似文献
246.
We determined how the species used for calibration affects the accuracy of in situ chlorophyll a (chl a) measurements by fluorometry using single-species cultures and natural phytoplankton populations from Winyah Bay, South Carolina,
USA. When a diatom was used for calibration, chl a in a dinoflagellate culture was overestimated by 66 ± 7%, whereas concentrations of a cryptophyte, chlorophyte, and cyanobacterium
were underestimated by 16 ± 20%, 40 ± 7%, and 71 ± 33%, respectively. In natural populations, the combination of species-specific
and environmentally induced variation in the ratio of fluorescence to chl a (F Chl−1) led to an overestimate by the in situ fluorometer of 40–169% for an April experiment and an underestimate of 4–50% in July.
Even when field samples were dominated by diatoms, environmental effects resulted in highly variable predictions of chl a. Thus, while a carefully selected calibration species can improve estimates of in vivo chl a in the laboratory, calibration of in situ fluorometers should be done with natural communities collected from the site of
interest. 相似文献
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