Detection of DNA damage in mussels and sea urchins exposed to crude oil using comet assay

The single-cell microgel electrophoresis assay or the comet assay was used to evaluate DNA damage of dispersed crude oil on sea urchins (Strongylocentrotus droebachiensis) and mussels (Mytilus edulis L.). Sea urchins were exposed to 0.06 and 0.25 mg/L dispersed crude oil in a continuous flow system, while the mussels were exposed to 0.015, 0.06 and 0.25 mg/L dispersed crude oil. Sea urchin coelomocytes and mussel haemocytes were sampled after 4 and 5 weeks exposure, respectively. In the sea urchin coelomocytes, there was a significant concentration-related increase in the percentage of DNA in comet tail. In mussel haemocytes, there was a significantly higher percentage of DNA in comet tail for all treatments compared to the control. The responses were concentration-related up to 0.06 mg/L oil. The two highest exposure concentrations of mussels were not significantly different from each other. These results indicate that the comet assay can be used for biomonitoring of DNA damage in marine invertebrates following oil contamination.     

论宁波东南港湾地区水资源的开发利用途径

本文在１９８９年对宁波市水资源进行调查的基础上，进一步分析了东南港湾地区水资源的特点及利用现状，并由此而提出了解决水源的对策。     

Co–rich Mn crusts from the Magellan Seamount cluster: the long journey through time

The Magellan seamounts began forming as large submarine shield volcanoes south of the equator during the Cretaceous. These volcanoes formed as a cluster on the small Pacific plate in a period when tectonic stress was absent. Thermal subsidence of the seafloor led to sinking of these volcanoes and the formation of guyots as the seamounts crossed the equatorial South Pacific (10–0°S) sequentially and ocean surface temperatures became too high for calcareous organisms to survive. Guyot formation was completed between about 59 and 45 Ma and the guyots became phosphatized at about 39–34 and 27–21 Ma. Ferromanganese crusts began formation as proto-crusts on the seamounts and guyots of the Magellan Seamount cluster towards the end of the Cretaceous up to 55 Ma after the formation of the seamounts themselves. The chemical composition of these crusts evolved over time in a series of steps in response to changes in global climate and ocean circulation. The great thickness of these crusts (up to 15–20 cm) reflects their very long period of growth. The high Co contents of the outer parts of the crusts are a consequence of the increasing deep circulation of the ocean and the resulting deepening of the oxygen minimum zone with time. Growth of the Co-rich Mn crusts in the Magellan Seamount cluster can be considered to be the culmination of a long journey through time.     

上新世——早更新世青藏高原北缘隆升的磁性地层学证据

对新疆叶城剖面西域砾岩及下伏第三纪地层的磁性地层学研究表明 ,西域砾岩的沉积时代为晚上新世至早更新世 ,磁性地层年龄为 3.5至 <1.8Ma。阿图什组沉积于早上新世 ,古地磁年龄为 4 .6～ 3.5 Ma。阿图什组以砂岩和粉砂岩为主夹薄层砾岩 ,为河流相及冲积扇前缘相。西域砾岩以厚层砾岩为主夹风成粉砂岩 ,为典型洪积—冲积扇堆积。西域砾岩的沉积反映了青藏高原北缘晚上新世至早更新世强烈的隆起和剥蚀     

早第三纪济阳湖盆的形成和演变

在构造和气候的综合作用下,本区在早第三纪成为一个大型裂谷湖盆,并在沙四、沙三和沙一期因受古太平洋海泛影响而具有"近海"湖盆的特征,其演变经历了初始形成-强烈扩张-晚期扩张-收缩干涸四个阶段,构成一个完整的湖侵-湖退旋回和湖泊早期形成-中期扩张-晚期收缩三个亚旋回。     

浅析地图集的发展与趋势

梳理了地图集的发展历史,总结了地图集的发展规律,并给出了地图集的发展趋势。     

Study on rain-runoff process in the peripheral mountainous area of the Sichuan Basin

Studies on rain-runoff process in the peripheral mountainous area of the Sichuan Basin, which is regarded as a key ecological shelter, will contribute to flood control and environmental protection for the Upper Yangtze River Basin. In two typical catchments--the Fujiang River Catchment and the Wujiang River Catchment, rainfall simulations have been conducted to study the rain-runoff processes of yellow soil and limestone soil in three types of land use--forestland, farmland and grassland. Results showed that （1） within the same rainfall process, overland flow occurs first on farmland, then on grassland, and finally on forestland; （2） soil surface coverage has a great impact on the occurrence and amount of overland flow. The runoff amount can increase 2-4 times after the coverage is removed; （3） the infiltration before the occurrence of overland flow will decrease because of higher gravel contents of soil, but it takes no effect on infiltration once overland flow becomes stable; （4） the runoff coefficient of the limestone soil forestland is greater than that of the yellow soil forest land, but less than that of the farmland; （5） three empirical infiltration models, including Horton＇ model, Kostiakov＇ model, and modified Kostiakov＇ model, were compared by using the observed results under rainfall simulation. The results showed that the Kostiakov＇ model performed better than both the Horton＇ model and modified Kostiakov model. According to the results of this research, the Kostiakov＇s model can be used to simulate rainfall infiltration when water erosion is modeled in the peripheral mountainous area of the Sichuan Basin.     

Geochemistry and environmental effect of cadmium in the super-large Jinding Pb-Zn deposit,Yunnan Province,China

The Jinding Pb-Zn deposit in Yunnan Province is the representative of a Cd-enriched area and mining activities lead to the release of Cd into the hypergenic ecosystem, resulting in Cd pollution. The concentrations of Cd vary greatly from one type to another type of rocks in the mining district. In the host rock, Cd concentrations range from 50×10^-6 to 650×10^-6 with an average of 310×10^-6. In primary ores, Cd concentrations range from 14×10^-6 to 2800×10^-6 with an average of 767×10^-6. However, in oxidized ores, Cd concentrations are highest, varying within the range of 110×10^-6 to 8200×10^-6 , averaging 1661×10^-6. It is shown that the oxidized ores are the main carder and environmental source of Cd. Leaching test showed that Pb/Zn ores are easy to oxidize and thereafter release Cd and other harmful elements. These leached elements in the leachate may be precipitated rapidly in the order of Zn〉Pb〉Cd. As for the concentration distribution of Cd in the Bijiang River, it is estimated to be 15.7 μg/L Cd in water, 49.3 mg/L in suspended substances, and 203.7 mg/L in sediments. The average value of Cd in soil from the polluted area is 83.0 mg/kg. Natural weathering of Cd-rich rocks and minerals imposes a potential environmental risk on the aquatic ecosystem of the Bijiang catchment.     

Simulation experiment on hydrocarbon generating potential of various source rocks on the northern margin of the Qaidam Basin,Qinghai, China

The northern margin of the Qaidam Basin is one of the main oil-gas exploration areas in western China, where source rocks are composed mostly of Middle and Lower Jurassic dark mud shale, carbargillite and coal. A large number of subsurface and outcrop samples differing in lithology with different types of organic matter were selected for resource evaluation, research and calculation. And among them, 13 samples were used for simulation experiment on hydrocarbon generating potential of various source rocks. At first, two kinds of heating modes were compared through simulation experiment, including single temperature-step heating and continual heating. Perhaps, the process of hydrocarbon generation and expulsion occurred naturally between a close system and an open system. In addition, the first heating mode was convenient, and all its reaction products were involved in the whole thermal evolution, and the final simulation experimental results were basically in consistency with the natural evolution trend Therefore, the first heating mode was adopted and the hydrocarbon yield of every sample was worked out. According to the type and lithology of organic matter and the hydrocarbon yield of samples for simulation experiment, hydrocarbon generation and expulsion mode with three kinds of lithology and five types of source rock has been established to provide the basis for hydrocarbon generation evaluation, research and resource calculation.     

Regional yield estimation for winter wheat with MODIS-NDVI data in Shandong, China

The significance of crop yield estimation is well known in agricultural management and policy development at regional and national levels. The primary objective of this study was to test the suitability of the method, depending on predicted crop production, to estimate crop yield with a MODIS-NDVI-based model on a regional scale. In this paper, MODIS-NDVI data, with a 250 m resolution, was used to estimate the winter wheat (Triticum aestivum L.) yield in one of the main winter-wheat-growing regions. Our study region is located in Jining, Shandong Province. In order to improve the quality of remote sensing data and the accuracy of yield prediction, especially to eliminate the cloud-contaminated data and abnormal data in the MODIS-NDVI series, the Savitzky–Golay filter was applied to smooth the 10-day NDVI data. The spatial accumulation of NDVI at the county level was used to test its relationship with winter wheat production in the study area. A linear regressive relationship between the spatial accumulation of NDVI and the production of winter wheat was established using a stepwise regression method. The average yield was derived from predicted production divided by the growing acreage of winter wheat on a county level. Finally, the results were validated by the ground survey data, and the errors were compared with the errors of agro-climate models. The results showed that the relative errors of the predicted yield using MODIS-NDVI are between −4.62% and 5.40% and that whole RMSE was 214.16 kg ha⁻¹ lower than the RMSE (233.35 kg ha⁻¹) of agro-climate models in this study region. A good predicted yield data of winter wheat could be got about 40 days ahead of harvest time, i.e. at the booting-heading stage of winter wheat. The method suggested in this paper was good for predicting regional winter wheat production and yield estimation.