Effect of isolation temperature on the characteristics of extracellular proteases produced by Antarctic bacteria

Protease-producing psychrotolerant bacteria were isolated from Antarctic biotopes on casein agar plates using different incubation temperatures. Most of the isolates were non-spore-forming Gram-negative motile rods with catalase activity, 30% were pigmented and none of them were glucose fermenters. All the strains were grown in liquid cultures at 20°C and protease secretion was tested using the azocasein method. Despite their capacity for production of a clear zone of hydrolysis in agar plates, some strains did not produce detectable levels of proteolytic activity in liquid cultures. The lowest apparent optimum temperature for protease activity found in culture supernatants was 40°C. Almost all the strains showed activation energy values about 10-20 kJ-mol⁻¹ lower than that observed for a mesophilic Subtilisin. Most of the proteases showed optimal activity at neutral or alkaline pH values and developed a multiple-band profile on gelatine-SDS-PAGE. It was observed that the lower the strain isolation temperature was, the more stongly cold-adapted–in terms of optimal temperature and activation energy–were the proteases produced by them. This dependence of the characteristics of the proteases on the isolation temperature is an important factor to take into account in the design of screening programmes directed towards the isolation of psychrotolerant bacteria able to produce proteases strongly or weakly adapted to work in the cold. The Antarctic area explored proved to be a promising source of proteolytic bacteria with potential use in industrial processes to be carried out at low or moderate temperatures.     

Clean and safe supply of fish and shellfish to clear the HACCP regulation by use of clean and cold deep ocean water in Rausu, Hokkaido, Japan

For the supply of fish and shellfish to consumers in fresh condition, clean handling after catch from the sea is essential. According to HACCP （Hazard Analysis and Critical Control Points）, it is important to meet such requirement by keeping fish and shellfish under a certain low temperature and clean conditions after catching. The deep ocean water （DOW） characterized by low temperature and cleanliness has been chosen for fish and shellfish handlings, particularly for salmon, cod, and sea urchin in Town ＇Rausu＇ in Hokkaido, Japan. DOW below 2.9℃ of an amount of nearly 5 000 m^3 is planned to be pumped up every day from a depth of about 350 m, and temporarily stored in a large simulated tank on land. DOW is then supplied to fish boats through hydrants distributed throughout the harbor and used for keeping salmon in clean and cold conditions. Ice made from DOW is also used for lowering temperature if necessary. DOW and ice made from DOW are also used during the transportation of fish and shellfish. The entire system is scheduled to be completed by the summer of 2005.     

Sulphur partitioning in sediments and biodeposits below mussel rafts in the Ria de Arousa (Galicia, NW Spain)

Forty four core samples were analyzed to determine sediment particle size, total organic carbon (TOC), total inorganic carbon (TIC), total nitrogen and total sulphur. Sequential extraction of S was also carried out, differentiating AVS (acid volatile sulphide), elemental S, organic S and pyrite S. The results obtained show that the presence of mussel rafts causes intense changes in the physicochemical composition and properties of the sea floor in the Ria de Arousa. The percentage of silt and clay, TOC, TIC and total N were significantly higher in the biodeposit than in the sediment. In contrast, there were no differences between the biodeposit and the sediment in terms of pH (8.0-8.7) and redox potential. The sediment and biodeposit were always anoxic, with values of redox potentials below -100 mV. In accordance with these conditions, the dominant fraction of S was pyrite S (FeS(2)). The AVS fraction and elemental S were present at low concentrations, except in the uppermost part of each core. Pyrite was relatively stable when the biodeposit and sediment were maintained in suspension for 8 days in oxic sea water; unlike the AVS fraction, which disappeared within a few hours.     

Accumulation mechanisms of micrometeorites in an ancient supraglacial moraine at Larkman Nunatak,Antarctica

We report the discovery of a large accumulation of micrometeorites (MMs) in a supraglacial moraine at Larkman Nunatak in the Grosvenor Mountains of the Transantarctic Range in Antarctica. The MMs are present in abundances of ~600 particles kg⁻¹ of moraine sediment and include a near-complete collection of MM types similar to those observed in Antarctic blue ice and within bare-rock traps in the Antarctic. The size distribution of the observed particles is consistent with those collected from snow collections suggesting the moraine has captured a representative collection of cosmic spherules with significant loss of only the smallest particles (<100 μm) by wind. The presence of microtektites with compositions similar to those of the Australasian strewn field suggests the moraine has been accumulating for 780 ka with dust-sized debris. On the basis of this age estimate, it is suggested that accumulation occurs principally through ice sublimation. Direct infall of fines is suggested to be limited by snow layers that act as barriers to accumulation and can be removed by wind erosion. MM accumulation in many areas in Antarctica, therefore, may not be continuous over long periods and can be subject to climatic controls. On the basis of the interpretation of microtektites as Australasian, Larkman Nunatak deposit is the oldest known supraglacial moraine and its survival through several glacial maxima and interglacial periods is surprising. We suggest that stationary ice produced by the specific ice flow conditions at Larkman Nunatak explains its longevity and provides a new type of record of the East Antarctic ice sheet.     

Impact of small-scale structures on estuarine circulation

We present a novel and challenging application of a 3D estuary-shelf model to the study of the collective impact of many small-scale structures (bridge pilings of 1 m × 2 m in size) on larger-scale circulation in a tributary (James River) of Chesapeake Bay. We first demonstrate that the model is capable of effectively transitioning grid resolution from ~?400 m down to ~?1 m near the pilings without introducing undue numerical artifact. We then show that despite their small sizes and collectively small area as compared to the total channel cross-sectional area, the pilings exert a noticeable impact on the large-scale circulation, and also create a rich structure of vortices and wakes around the pilings. As a result, the water quality and local sedimentation patterns near the bridge piling area are likely to be affected as well. However, when evaluating over the entire waterbody of the project area, the near field effects are weighed with the areal percentage which is small compared to that for the larger unaffected area, and therefore the impact on the lower James River as a whole becomes relatively insignificant. The study highlights the importance of the use of high resolution in assessing the near-field impact of structures.     

Dust provenance and its role as a potential fertilizing agent for the Okavango Delta,Botswana

Dust plays a globally important role in supplying biologically essential elements to landscapes underlain by nutrient-poor substrates. Here we show that dust may play a significant role in sustaining productivity in the vast wetlands of the Okavango Delta in southern Africa, one of the world's richest biodiversity hotspots. Dust accumulates preferentially on tree-covered islands in the seasonal swamps of the Delta, creating pockets of fine-grained, nutrient-rich material within the semi-arid landscape of the Kalahari Desert. Strontium and neodymium isotopes reveal that this dust likely originates predominantly from the Makgadikgadi salt pans, located 300 km away, and contributes 10–80% of the fine-grained material present in Okavango island soils. Surface material sourced from the Makgadikgadi Pans contains relatively high amounts of bioavailable phosphorus and iron, potentially influencing Okavango Delta biological productivity. We propose that long-term ecosystem productivity and nutrient availability in the Okavango may be strongly mediated by regional dust inputs. Understanding the influence of dust deposition on nutrient loads and biogeochemical cycling is thus critical for predicting the response of the Okavango Delta to future changes in climate. We suggest that dust inputs may play a significant role in the supply of nutrients to other large, global wetland systems located in dryland environments. © 2020 John Wiley & Sons, Ltd     

Electrical properties of Titan's surface from Cassini RADAR scatterometer measurements

We report regional-scale low-resolution backscatter images of Titan's surface acquired by the Cassini RADAR scatterometer at a wavelength of 2.18-cm. We find that the average angular dependence of the backscatter from large regions and from specific surface features is consistent with a model composed of a quasi-specular Hagfors term plus a diffuse cosine component. A Gaussian quasi-specular term also fits the data, but less well than the Hagfors term. We derive values for the mean dielectric constant and root-mean-square (rms) slope of the surface from the quasi-specular term, which we ascribe to scattering from the surface interface only. The diffuse term accommodates contributions from volume scattering, multiple scattering, or wavelength-scale near-surface structure. The Hagfors model results imply a surface with regional mean dielectric constants between 1.9 and 3.6 and regional surface roughness that varies between 5.3° and 13.4° in rms-slope. Dielectric constants between 2 and 3 are expected for a surface composed of solid simple hydrocarbons, water ice, or a mixture of both. Smaller dielectric constants, between 1.6 and 1.9, are consistent with liquid hydrocarbons, while larger dielectric constants, near 4.5, may indicate the presence of water-ammonia ice [Lorenz, R.D., 1998. Icarus 136, 344-348] or organic heteropolymers [Thompson, W.R., Squyres, S.W., 1990. Icarus 86, 336-354]. We present backscatter images corrected for angular effects using the model residuals, which show strong features that correspond roughly to those in 0.94-μm ISS images. We model the localized backscatter from specific features to estimate dielectric constant and rms slope when the angular coverage is within the quasi-specular part of the backscatter curve. Only two apparent surface features are scanned with angular coverage sufficient for accurate modeling. Data from the bright albedo feature Quivira suggests a dielectric constant near 2.8 and rms slope near 10.1°. The dark albedo feature Shangri-La is best fit by a Hagfors model with a dielectric constant close to 2.4 and an rms slope near 9.5°. From the modeled backscatter curves, we find the average radar albedo in the same linear (SL) polarization to be near 0.34. We constrain the total-power albedo in order to compare the measurements with available groundbased radar results, which are typically obtained in both senses of circular polarization. We estimate an upper limit of 0.4 on the total-power albedo, a value that is significantly higher than the 0.21 total albedo value measured at 13 cm [Campbell, D., Black, G., Carter, L., Ostro, S., 2003. Science 302, 431-434]. This is consistent with a surface that has more small-scale structure and is thus more reflective at 2-cm than 13-cm. We compare results across overlapping observations and observe that the reduction and analysis are repeatable and consistent. We also confirm the strong correlations between radar and near-infrared images.     

Crustal properties of Mercury by morphometric analysis of multi‐ring basins on the Moon and Mars

Abstract— We use satellite altitude free‐air and terrain gravity correlations to differentiate regional variations in crustal viscosity and transient cavity diameters of impact basins on the Moon and Mars that we combine with surface roughness for a rheological assessment of the crust of Mercury. For the Moon and Mars, we separate the free‐air anomalies into terrain‐correlated and terrain‐decorrelated components using the spectral properties of the free‐air and computed terrain gravity effects. Adjusting the terrain effects for the terrain‐correlated anomalies yields compensated terrain effects that we evaluate for crustal thickness variations of the impact basins. We interpret the terrain‐correlated anomalies for uncompensated elements of the crustal thickness variations that we find are strongly correlated with the distribution of basin rings from photogeologic analyses. Hence, we estimate the transient cavity diameter from the innermost diameter of the gravity‐inferred rings. Comparing these diameters with the related crustal thickness estimates clearly differentiates regional variations in the crustal rheologies. For the Moon, the analysis points to a farside crust that was significantly more rigid than the nearside crust during bombardment time. For Mars, the growth in transient cavity diameters with apparent crustal age also reflects increased viscosity due to crustal cooling. These results are also consistent with local estimates of surface roughness developed from the root‐mean‐squared topography over 64 times 64° patches centered on the basins. Hence for Mercury where gravity observations are lacking, rheological inferences on its crust may result from comparing photometric estimates of transient cavity diameter and local surface roughness with the lunar and martian estimates. These results for the Beethoven Basin, a typical multi‐ring impact feature of Mercury, suggest that the viscosity of the mercurian crust was relatively great during bombardment time. This enhanced rigidity, despite crustal temperatures that were probably much hotter than those of the Moon and Mars, may reflect an extremely dry crust for Mercury in its early development.     

Cosmological Evolution of Supergiant Star-Forming Clouds

We present the results of very high resolution CDM simulations of galaxy formation designed to follow the formation and evolution of self-gravitating, supergiant star-forming clouds. We find that the mass spectrum of these clouds is identical to that of globular clusters and GMCs; dN/dM ∝ M ^-1.7 ± 0.1. This revised version was published online in September 2006 with corrections to the Cover Date.     

Extension of a fluctuating plume model of tracer dispersion to a sheared boundary layer and to a large array of obstacles

Fluctuating plume models provide a useful conceptual paradigm in the understanding of plume dispersion in a turbulent flow. In particular, these models have enabled analytical predictions of higher-order concentration moments, and the form of the one-point concentration probability density function (PDF). In this paper, we extend the traditional formalism of these models, grounded in the theory of homogeneous and isotropic turbulent flow, to two cases: namely, a simple sheared boundary layer and a large array of regular obstacles. Some very high-resolution measurements of plume dispersion in a water channel, obtained using laser-induced fluorescence (LIF) line-scan techniques are utilised. These data enable us to extract time series of plume centroid position (plume meander) and dispersion in the relative frame of reference in unprecedented detail. Consequently, experimentally extracted PDFs are able to be directly compared with various theoretical forms proposed in the literature. This includes the PDF of plume centroid motion, the PDF of concentration in the relative frame, and a variety of concentration moments in the absolute and relative frames of reference. The analysis confirms the accuracy of some previously proposed functional forms of model components used in fluctuating plume models, as well as suggesting some new forms necessary to deal with the complex boundary conditions in the spatial domain.