Utilizing in vivo nuclear magnetic resonance spectroscopy to study sublethal stress in aquatic organisms

In vivo nuclear magnetic resonance spectroscopy (NMR) is a powerful technique for characterizing the sublethal actions of physical and chemical stressors in live, intact organisms. In particular, 31P NMR is ideal for observing perturbations to cellular energetics since critical metabolite concentrations, including phosphagens, ATP and inorganic phosphate (Pi), can be measured non-invasively and in real time. This technique's versatility is demonstrated not only in the diversity of organisms that can be studied, but also in its broad-ranging applicability to environmental research. Illustrative studies include the actions of copper in adult red abalone (Haliotis rufescens) and changes in energetically important metabolites in developing medaka embryos (Oryzias latipes). Advantages and disadvantages of in vivo NMR will be discussed.     

Analysis of the 16S-23S rDNA intergenic spacers (IGSs) of marine vibrios for species-specific signature DNA sequences

Vibrios are widespread in the marine environment and a few pathogenic species are known to be commonly associated with outbreaks of diarrheal diseases in humans due to the consumption of raw or improperly cooked seafood. However, there are also many Vibrio species which are potentially pathogenic to vertebrate and invertebrate aquatic animals, and of which little is known. In an attempt to develop rapid PCR detection methods for these latter class of vibrios, we have examined the 16S-23S intergenic spacers (IGSs) of 10 lesser-known Vibrio species and successfully developed species-specific primers for eight of them--Vibrio costicola, V. diazotrophicus, V. fluvialis, V. nigripulchritudo, V. proteolyticus, V. salmonicida, V. splendidus and V. tubiashii. The IGS amplicons were amplified using primers complementary to conserved regions of the 16S and 23S rRNA genes, and cloned into plasmid vectors and sequenced. Analysis of the IGS sequences showed that 37 ribosomal RNA (rrn) operons representing seven different IGS types have been cloned from the 10 vibrios. The three IGS types--IGS(0), IGS(IA) and IGS(Glu)--were the most prevalent forms detected. Multiple alignment of representative sequences of these three IGS types from different Vibrio species revealed several domains of high sequence variability, which were used to design species-specific primers for PCR. The specificity of the primers were evaluated using total DNA prepared from different Vibrio species and bacterial genera. The results showed that the PCR method can be used to reliably detect eight of the 10 Vibrio species in marine waters in this study.     

美国诺阿卫星计划：迈向21世纪的新进展

文章译自美国国家海洋大气局（ＮＯＡＡ）、国家环境卫星数据及服务局（ＮＥＳＤＩＳ）局长Ｒｏｂｅｒｔ Ｓ．Ｗｉｎｏｋｕｒ的报告。文章介绍了迈向２１世纪的美国诺阿环境卫星坟墓和卫星遥感应用技术的进展，使读者了解未来美国极轨气象卫星系统、极具前景的三维温，湿变量卫星遥感反演以及静止气象卫星最新遥感应用技术发展的前景。     

Detection of Extended Thermal Infrared Emission around the Vega-like Source HD 141569

We report the detection of extended IR emission at 10.8 and 18.2 μm around the Vega-like source HD 141569. Mid-IR imaging with OSCIR on Keck II shows emission from dust extending out to 100 AU from the B9.5 Ve star. Our modeling of the dust places an upper limit of approximately 2 μm on the diameter of the mid-IR-emitting particles if they are Mie spheres of astronomical silicates. Comparison of our mid-IR images to the near-IR (1.1 μm) NICMOS images of HD 141569 (Weinberger et al. 1999) shows that the mid-IR emission originates at smaller distances from the star than the scattered near-IR light, as also previously observed for the archetype Vega-like source beta Pictoris.     

Toxicological and chemical assessment of ordinance compounds in marine sediments and porewaters

Toxicological and chemical studies were performed with a silty and a sandy marine sediment spiked with 2,6-dinitrotoluene (2,6-DNT), 2,4,6-trinitrophenylmethylnitramine (tetryl), or 2,4,6-trinitrophenol (picric acid). Whole sediment toxicity was analyzed by the 10-day survival test with the amphipod Ampelisca abdita, and porewater toxicity tests assessed macro-algae (Ulva fasciata) zoospore germination and germling growth, sea urchin (Arbacia punctulata) embryological development, and polychaete (Dinophilus gyrociliatus) survival and reproduction. Whole sediments spiked with 2,6-DNT were not toxic to amphipods. The fine-grained sediment spiked with tetryl was also not acutely toxic. The tetryl and picric acid LC50 values in the sandy sediment were 3.24 and 144 mg/kg dry weight, respectively. The fine-grained sediment spiked with picric acid generated a U-shaped concentration-response curve in the amphipod test, with increased survival both in the lowest and highest concentration. Grain-size distribution and organic carbon content strongly influenced the behavior of ordnance compounds in spiked sediments. Very low concentrations were measured in some of the treatments and irreversible binding and biodegradation are suggested as the processes responsible for the low measurements. Porewater toxicity varied with its sedimentary origin and with ordnance compound. The sea urchin embryological development test tended to be the least sensitive. Tetryl was the most toxic chemical in all porewater tests, and picric acid the least toxic. Samples spiked with 2,6-DNT contained a degradation product identified as 2-methyl-3-nitroaniline (also known as 2-amino-6-nitrotoluene), and unidentified peaks, possibly degradation products, were also seen in some of the picric acid- and tetryl-spiked samples. Degradation products may have played a role in observed toxicity.     

A field experimental study on recolonization and succession of subtidal macrobenthic community in sediment contaminated with industrial wastes

A field experiment was carried out in Hong Kong to study the patterns of recolonization and succession of subtidal macrobenthos in defaunated sediment contaminated with industrial wastes and to determine the time required for benthic recovery in the industrial-contaminated sediment. A total of 50 species was found with an average of 172 animals/tray and 24 species/tray recorded one month after deployment. Initial colonizers were predominantly polychaetes (96 animals/tray, accounting for 55.7%) and gastropods (47 animals/tray, accounting for 27.2%). Abundance of macrobenthos increased quickly to a peak (505 animals/tray) after four months, declined afterwards, and increased again till the end of the experiment. Species number peaked (57 species/tray) in the same month as abundance did, and gradually declined thereafter. Abundance, species number and diversity were significantly lower in the industrial-contaminated sediment as compared to the controls during the early successional stages, indicating the harmful effects of industrial wastes on recolonization and succession of macrobenthos. Although no significant differences in community parameters between the industrial-contaminated and the control sediments were found after eleven months, significant difference in species composition still existed after fourteen months, showing a relatively long-term impact of industrial wastes on macrobenthic community structure.     

美国蒙大拿卡尼湾干谷钼矿的流体包裹体和相平衡研究：CO2对辉钼矿…

卡尼湾干谷的钼矿床以网状脉化和矽卡岩化为特征。然而不同的围岩有明显不同的矿物组合。在卡尼湾岩株中，辉钼矿产于典型的石英－钾长石或石英－白云母脉中。在围岩中，辉钼矿产于绿泥石＋黄铁矿＋磁铁矿中，其中包含有不同量的石英、方解石和绿帘石。包含于石英和萤石中的原始流体包裹体有水溶液、液体ＣＯ２和气体ＣＯ２。     

产美考古磁学数据库

北美洲的考古磁学数据库是采用一个关系数据库管理系统编辑完成的,该数据库中现有１８７５个古地磁方向和１５１个古地磁场强度的结果。