冬季刺参养殖环境与肠道内细菌菌群的研究

运用传统细菌分离培养与分子生物学技术相结合的方法,对2008年11月至2009年1月冬季刺参(Apostichopus japonicus Liao)养殖池塘环境(养殖水、底泥、附着基)及刺参肠道内的细菌菌群进行了分析。应用平板稀释涂布培养计数法测得刺参养殖池塘水体、底泥、附着基和肠道细菌数量分别为0.75×102～1.4×104cfu/mL、8.7×104～8.1×105cfu/g、3.8×105～2.8×106cfu/g、7.1×105～1.5×107cfu/g;根据形态学差异从培养所得的细菌中筛选得到22株菌,用限制性内切酶Rsa I和Msp I对所分离菌株进行ARDRA(Amplified rDNA Restriction Analysis)分析,这22株菌被分为8种不同的分类单元(Operational Taxonomic Unit,OTU),其中OTU2与OTU3所包含的菌株分别占分离菌株种数的30%和20%;此外,作者对不同环境培养所得的优势度最高的细菌进行分子鉴定分析。结果表明:水环境中优势菌为施氏假单胞菌(Pseudomonas stutzeri)、门多萨假单胞菌(Pseudomonas mendocina)及巨大芽孢杆菌(Bacillus megaterium),沉积物中优势菌为巨大芽孢杆菌(B.megaterium)、施氏假单胞菌(P.stutzeri)、苏云金芽孢杆菌(Bacillus thuringiensis),附着基中优势菌为巨大芽孢杆菌(B.megaterium)、苏云金芽孢杆菌(B.thuringiensis),灿烂弧菌(Vibrio splendidus),肠道中优势菌为巨大芽孢杆菌(B.megaterium)、苏云金芽孢杆菌(B.thuringiensis),灿烂弧菌(V.splendidus)、施氏假单胞菌(P.stutzeri)。通过对冬季刺参池塘细菌菌群多样性分析和优势菌鉴定,为筛选低温益生菌和防治刺参疾病提供了有益参考。     

台湾海峡及周边海域水色光谱特性分析

利用2006—2007年4次908计划ST06区块海洋光学调查的数据和MODIS水色遥感数据,对台湾海峡及周边海域的水色光谱特性进行了研究和分析。结果显示该海域的水色光谱主要有4个类型:类型1光谱峰值出现在580 nm附近,谱峰两侧遥感反射率随波长变化迅速减小,主要分布在紧靠中国大陆的海域;类型2光谱峰值出现在540～560 nm之间,具有谱峰宽的特点,主要分布在台湾海峡靠近大陆的一侧;类型3光谱与大洋水体光谱类似,主要分布在台湾西南的南海区域和吕宋海峡区域;类型4光谱在.450 nm附近出现极小值,在500～600 nm之间出现峰值,主要出现在台湾海峡中部和靠近台湾岛的一侧。其中类型2和4水体分布具有明显的季节特征。类型3、4、2、1对应的叶绿素a浓度和悬浮泥沙浓度呈上升趋势,对应的透明度依次降低,该海域以二类水体为主。     

全球海洋环流模式中上层海洋对表面强迫的响应和调整 I. 年际变率

利用一个较高分辨率的全球海洋环流模式在COADS1945～1993年逐月平均资料的强迫下对海温和环流场进行了模拟试验,研究了全球热带海洋(主要是热带太平洋)海温和环流场的年际变化特征及模式ENSO冷暖事件演变的控制机理.结果表明,模式成功地再现了和观测一致的海温和环流的年际变化以及ENSO演变特征.其中热带印度洋年际SST变率的主要模态表现为与ENSO相联系的海盆尺度的一致性增暖或变冷现象,次级模态为热带印度洋偶极子模态;热带大西洋的SST年际变率表现为类ENSO的年际振荡现象.在热带太平洋,SST年际变化主要表现为ENSO型,环流的年际变率表现为与ENSO相对应的热带海洋质量循环圈的年际异常.对应于暖(冷)事件,前期赤道海洋垂直环流圈显示出减弱(增强)的特征.其中南赤道流异常的位相较Nino3区海温总体要超前5个月左右的时间;赤道上翻流异常的位相在表层要超前4个月,并随时间由上至下扩展;赤道潜流的异常则显示出东传特征,其中最早的较为显著的异常发生ENSO成熟前3个月180°附近.在模式ENSO冷暖事件的演变过程中,次表层海温异常沿赤道的东传起了关键作用,模式的ENSO模态主要表现为"时滞振子"模态.     

HPLC法同时测定滋阴温阳活血颗粒中两种指标性成分的研究

目的：建立滋阴温阳活血颗粒中两种指标性成分（腺苷和山奈酚-3-O-β-D-葡萄糖苷）的同时测定方法。方法：采用HPLC法,选用phenomex Kinetex C_{18色谱柱（4.6mm×250mm,5μm）,0.1%磷酸-乙腈为流动相,梯度洗脱,流速1.0ml/min,柱温30℃,进样量10μm,检测波长254nm;以腺苷和山奈酚-3-O-β-D-葡萄糖苷为指标性成分,共测定10批滋阴温阳活血颗粒。结果：10批颗粒中,腺苷的含量在2.706～4.132mg/g范围内,山奈酚-3-O-β-D-葡萄糖苷的含量在3.692～4.948mg/g范围内。结论：本研究所建立方法快速、准确、高效,能够对滋阴温阳活血颗粒的质量进行科学评价。}     

东太平洋海隆13°N表层含金属沉积物物质组成与元素赋存状态

为了解不同类型含金属沉积物在物质组成和元素赋存状态的差异,对东太平洋海隆13°N洋中脊两侧表层含金属沉积物(站号:E271和E53)进行了矿物学、地球化学分析,顺序提取实验,并与前人对轴部表层沉积物(站号:17A-EPR-TVG1)的研究结果作了对比分析。研究结果表明, E271和E53是远端含金属沉积物,由非浮力热液羽状流中颗粒物沉降所形成的;17A-EPR-TVG1沉积物是近喷口含金属沉积物,由黑烟囱或者热液硫化物丘状体崩塌、堆积,或者由热液羽状流中Fe-Mn氧化物和硫化物快速沉淀而形成的,近喷口沉积物比远端沉积物更富集Fe、Cu和Zn等元素。元素在两种含金属沉积物中的赋存状态基本相同,除了Fe,Cu,Zn,Mo和稀土元素(REE)等元素在远端沉积物中主要存在于Fe-Mn氧化物相,在近喷口沉积物中主要在残留相中。远端沉积物中REE页岩标准化配分模式与海水相似,表明REE主要来自海水,而近喷口沉积物中REE配分模式与热液流体相似,说明REE以高温热液流体来源为主。相关研究结果加深了对热液沉积作用研究的认识。     

Smectite formation in metalliferous sediments near the East Pacific Rise at 13°N

A 43 cm long E271 sediment core collected near the East Pacific Rise(EPR) at 13°N were studied to investigate the origin of smectite for understanding better the geochemical behavior of hydrothermal material after deposition.E271 sediments are typical metalliferous sediments. After removal of organic matter, carbonate, biogenic opal,and Fe-Mn oxide by a series of chemical procedures, clay minerals(2 μm) were investigated by X-ray diffraction,chemical analysis and Si isotope analysis. Due to the influence of seafloor hydrothermal activity and close to continent, the sources of clay minerals are complex. Illite, chlorite and kaolinite are suggested to be transported from either North or Central America by rivers or winds, but smectite is authigenic. It is enriched in iron, and its contents are highest in clay minerals. Data show that smectite is most likely formed by the reaction of hydrothermal Fe-oxyhydroxide with silica and seawater in metalliferous sediments. The Si that participates in this reaction may be derived from siliceous microfossils(diatoms or radiolarians), hydrothermal fluids, or detrital mineral phases. And their δ30 Si values are higher than those of authigenic smectites, which implies that a Si isotope fractionation occurs during the formation because of the selective absorption of light Si isotopes onto Feoxyhydroxides. Sm/Fe mass ratios(a proxy for overall REE/Fe ratio) in E271 clay minerals are lower than those in metalliferous sediments, as well as distal hydrothermal plume particles and terrigenous clay minerals. This result suggests that some REE are lost during the smectite formation, perhaps because their large ionic radii of REE scavenged by Fe-oxyhydroxides preclude substitution in either tetrahedral or octahedral lattice sites of this mineral structure, which decreases the value of metalliferous sediments as a potential resource for REE.     

PS Vir:a short-period solar-like contact binary

We present multi-color photometric observations and a one-dimensional spectrum, acquired from March 2016 to May 2017, for the short-period eclipsing binary PS Vir, by using the 2.16-m,85-cm and 60-cm telescopes at Xinglong station, which is administered by National Astronomical Observatories, Chinese Academy of Sciences. The spectral type was determined as G2V from the onedimensional spectrum. The photometric solution was reduced from BV Rc light curves. The results imply that PS Vir is a W-subtype contact binary with a mass ratio of q = 0.305(±0.008) and a fill-out factor of f = 14.4(±1.8)%. The orbital period may be undergoing a cyclic oscillation with an amplitude of A = 0.0027(±0.0001) d and a modulated period of 11.7(±0.2) yr, which may result from the light-time effect due to a third body. The lower limit on mass for the assumed component is 0.12 M⊙.Moreover, the more massive component of PS Vir may be a bit more evolved star as determined from the mass-luminosity diagram.     

LAMOST CCD camera-control system based on RTS2

The Large Sky Area Multi-Object Fiber Spectroscopic Telescope(LAMOST) is the largest existing spectroscopic survey telescope, having 32 scientific charge-coupled-device(CCD) cameras for acquiring spectra. Stability and automation of the camera-control software are essential, but cannot be provided by the existing system. The Remote Telescope System 2 nd Version(RTS2) is an open-source and automatic observatory-control system. However, all previous RTS2 applications were developed for small telescopes. This paper focuses on implementation of an RTS2-based camera-control system for the 32 CCDs of LAMOST. A virtual camera module inherited from the RTS2 camera module is built as a device component working on the RTS2 framework. To improve the controllability and robustness, a virtualized layer is designed using the master-slave software paradigm, and the virtual camera module is mapped to the 32 real cameras of LAMOST. The new system is deployed in the actual environment and experimentally tested. Finally, multiple observations are conducted using this new RTS2-frameworkbased control system. The new camera-control system is found to satisfy the requirements for automatic camera control in LAMOST. This is the first time that RTS2 has been applied to a large telescope, and provides a referential solution for full RTS2 introduction to the LAMOST observatory control system.     

Fast adsorption of microcystin-LR by Fe(III)-modified powdered activated carbon

Journal of Oceanology and Limnology - Microcystins (MCs) are cyclic hepatotoxic peptides produced by the bloom-forming cyanobacterium Microcystis and present a public health hazard to humans and...     

一种基于熵权法的小波去噪复合评价指标

传统的评价指标在真值未知的情况下不能满足小波去噪质量评价的要求。为此,借助变化率特征重新构建均方根误差变化量和平滑度变化量两个指标,利用熵权法定权将归一化后的两个指标线性组合,所得到的新指标即为复合评价指标。该方法借助指标的变化率随分解层数的增加表现出明显的收敛特性来确定去噪最优分解层数。实验表明,该方法能够在真值未知的情况下准确地指导小波分解,确定去噪最优分解层数,从而达到最优去噪效果。