Existing spatial clustering methods primarily focus on points distributed in planar space. However, occurrence locations and background processes of most human mobility events within cities are constrained by the road network space. Here we describe a density-based clustering approach for objectively detecting clusters in network-constrained point events. First, the network-constrained Delaunay triangulation is constructed to facilitate the measurement of network distances between points. Then, a combination of network kernel density estimation and potential entropy is executed to determine the optimal neighbourhood size. Furthermore, all network-constrained events are tested under a null hypothesis to statistically identify core points with significantly high densities. Finally, spatial clusters can be formed by expanding from the identified core points. Experimental comparisons performed on the origin and destination points of taxis in Beijing demonstrate that the proposed method can ascertain network-constrained clusters precisely and significantly. The resulting time-dependent patterns of clusters will be informative for taxi route selections in the future. 相似文献
This study was carried out to investigate the biogenic amines(BAs), physicochemical property and microorganisms in dry salted fish, a traditional aquatic food consumed in China. Forty three samples of dry salted fish were gathered from retail and wholesale markets and manufacturers, which had been produced in various regions in China. Cadaverine(CAD) and putrescine(PUT) were quantitatively the most common biogenic amines. About 14% of the samples exceeded the histamine content standards established by the FDA and/or EU. The highest histamine content was found in Silver pomfret(Pampus argenteus)(347.79 mg kg-1). Five of forty three samples exceeded the acceptable content of TYR(100 mg kg-1), and 23.26% of dried-salted fish contained high contents of biogenic amines(above 600 mg kg-1). In addition, species, regions, pickling processes and drying methods made the physicochemical property, microorganisms and biogenic amines in dry salted fish to be different to some extents. The total plate count(TPC) was much higher than that of total halophilic bacteria in all samples. The biogenic amines, physicochemical property and microbiological counts exhibited large variations among samples. Furthermore, no significant correlation between biogenic amines and physicochemical property and TPC was observed. This study indicated that dry salted fish may still present healthy risk for BAs, depending on the processing methods, storage conditions among others. 相似文献
Hydrogeology Journal - Quantifying groundwater/surface-water interactions is essential for managing water resources and revealing contaminant fate. There has been little concern on the exchange... 相似文献
Journal of Geographical Sciences - Lake ice phenology is considered a sensitive indicator of regional climate change. We utilized time series information of this kind extracted from a series of... 相似文献
We analyzed the spatial local accuracy of land cover (LC) datasets for the Qiangtang Plateau, High Asia, incorporating 923 field sampling points and seven LC compilations including the International Geosphere Biosphere Programme Data and Information System (IGBPDIS), Global Land cover mapping at 30 m resolution (GlobeLand30), MODIS Land Cover Type product (MCD12Q1), Climate Change Initiative Land Cover (CCI-LC), Global Land Cover 2000 (GLC2000), University of Maryland (UMD), and GlobCover 2009 (Glob-Cover). We initially compared resultant similarities and differences in both area and spatial patterns and analyzed inherent relationships with data sources. We then applied a geographically weighted regression (GWR) approach to predict local accuracy variation. The results of this study reveal that distinct differences, even inverse time series trends, in LC data between CCI-LC and MCD12Q1 were present between 2001 and 2015, with the exception of category areal discordance between the seven datasets. We also show a series of evident discrepancies amongst the LC datasets sampled here in terms of spatial patterns, that is, high spatial congruence is mainly seen in the homogeneous southeastern region of the study area while a low degree of spatial congruence is widely distributed across heterogeneous northwestern and northeastern regions. The overall combined spatial accuracy of the seven LC datasets considered here is less than 70%, and the GlobeLand30 and CCI-LC datasets exhibit higher local accuracy than their counterparts, yielding maximum overall accuracy (OA) values of 77.39% and 61.43%, respectively. Finally, 5.63% of this area is characterized by both high assessment and accuracy (HH) values, mainly located in central and eastern regions of the Qiangtang Plateau, while most low accuracy regions are found in northern, northeastern, and western regions.
Reversible protein phosphorylation, catalyzed by protein kinases and phosphatases, is an important and versatile mechanism by which eukaryotic cells regulate almost all the signaling processes. Protein phosphatase 1 (PP1) is the first and well-characterized member of the protein serine/threonine phosphatase family. In the present study, a full-length cDNA encoding the beta isoform of the catalytic subunit of protein phosphatase l(PPlcb), was for the first time isolated and sequenced from the skin tissue of flatfish turbot Scophthalmus maximus, designated SmPPlcb, by the rapid amplification of cDNA ends (RACE) technique. The cDNA sequence of SmPPlcb we obtained contains a 984 bp open reading frame (ORF), flanked by a complete 39 bp 5' untranslated region and 462 bp 3' untranslated region. The ORF encodes a putative 327 amino acid protein, and the N-terminal section of this protein is highly acidic, Met-Ala-Glu-Gly-Glu-Leu-Asp-Val-Asp, a common feature for PP1 catalytic subunit but absent in protein phosphatase 2B (PP2B). And its calculated molecular mass is 37 193 Da and pI 5.8. Sequence analysis indicated that, SmPPlcb is extremely conserved in both amino acid and nucleotide acid levels compared with the PPlcb of other vertebrates and invertebrates, and its Kozak motif contained in the 5'UTR around ATG start codon is GXXAXXGXXATGG, which is different from mammalian in two positions A6 and G3, indicating the possibility of different initiation of translation in turbot, and also the 3'UTR of SmPPlcb is highly diverse in the sequence similarity and length compared with other animals, especially zebraf'lsh. The cloning and sequencing of SmPPlcb gene lays a good foundation for the future work on the biological functions of PP1 in the flatfish turbot. 相似文献
It is not clear how the water sources are used by trees in typical karst areas of Guangxi, China. Trees growing on rigid carbonate
rock can primarily use spring water, precipitation-recharged soil water or a mixture of the two. In this study, the Nongla
karst dynamic monitoring station in the southwest of Guangxi, China, was observed. To determine the water sources accessed
by trees growing on rigid carbonate rock, seasonal change of isotope ratios (δ18O and δ D) in twig sap, soil water, rainfall and spring water were measured. Stem water samples plotted to the right of the
meteoric water line indicates utilization of water sources subject to evaporative isotopic enrichment. Trees growing on rigid
carbonate rock in the southwest of China used rainfall directly in the rainy season. Adult trees tended to use deeper water
stored in the epikarst zone, while the young ones used soil water when precipitation decreased. In the dry season, all trees
sampled depend mostly on water stored in the epikarst zone. Spring water was the major water source for all of the tree species
investigated in the dry season. In conclusion, these data indicate that trees growing on rigid carbonate rock in southwest
China depend mostly on water stored in the epikarst zone. Therefore, water storage in the epikarst zone is the essential water
source for trees growing on carbonate rock in southwest China. 相似文献