In this study, a systematic survey of cultural airborne fungi was carried out in the occurrence environments of wall paintings that are preserved in the Tiantishan Grottoes and the Western Xia Museum, China. A bio-aerosol sampler was used for sampling in four seasons in 2016. Culture-dependent and -independent methods were taken to acquire airborne fungal concentration and purified strains; by the extraction of genomic DNA, amplification of fungal ITS rRNA gene region, sequencing, and phylogenetic analysis, thereafter the fungal community composition and distribution characteristics of different study sites were clarified. We disclosure the main environmental factors which may be responsible for dynamic changes of airborne fungi at the sampling sites. The concentration of cultural airborne fungi was in a range from 13 to 1,576 CFU/m3, no significant difference between the two sites at the Tiantishan Grottoes, with obvious characteristics of seasonal variation, in winter and spring were higher than in summer and autumn. Also, there was a significant difference in fungal concentration between the inside and outside of the Western Xia Museum, the outside of the museum was far more than the inside of the museum in the four seasons, particularly in the winter. Eight fungal genera were detected, including Cladosporium, Penicillium, Alternaria, and Filobasidium as the dominant groups. The airborne fungal community structures of the Tiantishan Grottoes show a distinct characteristic of seasonal variation and spatial distribution. Relative humidity, temperature and seasonal rainfall influence airborne fungal distribution. Some of the isolated strains have the potential to cause biodeterioration of ancient wall paintings. This study provides supporting information for the pre-warning conservation of cultural relics that are preserved at local sites and inside museums. 相似文献
In this study,three methods were used to analyze 17 large-scale local high-temperature regions with durations exceeding 2 h within magnetic clouds(MCs)observed by advanced composition explorer from 1998 to 2008.Results show that five of these large-scale regions may have been caused by flare heating;seven of the regions may have been caused by nonuniform expansion when MCs propagated in the solar-terrestrial space;four large-scale high temperature regions may likely result from combined non-uniform expansion and flare heating;and only one large-scale local high-temperature region was not related to either flare heating nor non-uniform expansion.No evidence indicated that magnetic reconnection occurred or had occurred within the high-temperature regions.Based on our results,we infer that such local high-temperature phenomena within MCs are caused primarily as a result of flare heating and non-uniform expansion,either separately or jointly,and that magnetic reconnection plays only a minor role in the formation of high-temperature regions. 相似文献