罗氏沼虾线粒体锰超氧化物歧化酶的克隆表达与功能研究

罗氏沼虾(Macrobrachium rosenbergii)是我国重要的经济虾类之一。近年来, 细菌性感染造成罗氏沼虾养殖业病害频发, 经济损失巨大。因此了解其免疫机制对于指导疾病防控至关重要。线粒体锰超氧化物歧化酶(mitochondrial manganese superoxide dismutase, mMnSOD)被认为是抵御氧化应激的第一道防线, 在先天性免疫中具有至关重要的作用。目前, 甲壳动物中mMnSOD的免疫功能尚不清楚。为此, 本研究克隆了罗氏沼虾mMnSOD基因(mMnSOD of M. rosenbergii, MrmMnSOD), 制备了多克隆抗体, 分析了嗜水气单胞菌(Aeromonas hydrophila)感染下, MrmMnSOD在不同组织中的表达模式。结果显示, 肝胰腺和肠组织中MrmMnSOD的基因表达水平分别在感染后3h和6h达到最大; 组织免疫荧光分析显示, 肝胰腺和肠组织中MrmMnSOD均在感染后12h达到最大荧光强度。以上结果表明, mMnSOD参与了罗氏沼虾对嗜水气单胞菌的免疫应激反应。进一步的蛋白抑菌实验表明, MrmMnSOD可显著抑制大肠杆菌(Escherichia coli)、嗜水气单胞菌、副溶血弧菌(Vibrio parahaemolyticus)、金黄色葡萄球菌(Staphylococcus aureus)和无乳链球菌(Streptococcus agalactiae)的生长, 推测该蛋白可能属于免疫相关分子, 可通过抑菌反应发挥免疫功能。当前的研究结果进一步丰富了甲壳动物先天性免疫基础理论, 也可为今后罗氏沼虾的病害防控和药物研发提供新的靶点参考。     

Formation and evolution of sand deserts in Xinjiang,Northwest China：Ⅰ. Provenances of desert sands

Sandy desert landscape is a geological product of arid climate and abundant sand materials supply in natural conditions, therefore the formation of sandy desert is an ideal studying object for understanding the interaction between various stratigraphic/epigenetic spheres of the earth system. However, until now, the knowledge about the provenance and formation of these deserts in Xinjiang, Central Asia is diverse and inconsistent, a systematic review is necessary. Ancient aeolian sand sediments in the internal areas of these deserts and their twin dust deposits in the Cenozoic strata surrounding the Xinjiang deserts are direct and indirect clues reflecting the provenance of sand materials and the formation and evolution of deserts. Based on the geochemical, mineralogical and isotopic evidences of desert sands and relevant deposits in the peripheral regions, this paper reviews the research progress on the development of the sandy deserts in Xinjiang, Northwest China. Many proofs proposed that desert sands in Xinjiang were mainly sourced from the ancient pluvial, alluvial and fluvial sediments and were eventually achieved because of the local sand supply. It pointed out that the settings of tectonic structure in Xinjiang had made sediments both in the Tarim Basin and the Junggar Basin being influenced greatly by regional hydrological system and aeolian processes originated from the planetary wind system of westerly, the East Asian winter monsoon and the topographical mountain-valley winds. However, the directions of transportation paths are different between the hydrological dynamics and the aeolian dynamics, which are decided by the slantwise structures of the basins tectonics and the regional atmospheric circulation routes, respectively, and have determined anisotropies of the transport pathway of these sediments delivered by different agents. This results in a geological cycle of detrital sediment transportation in the two large basins and thus largely increases the degree of sediment mixing, alternation and recycle between younger and older sediment sources, as well as the complexity and diversity of sand provenances.     

南京市居民网络消费的影响因素及空间特征简

在互联网络和信息技术的影响下,网上购物、团购等基于电子商务平台的网络消费方式得到极大发展,网络消费逐渐成为城市居民消费选择的重要方式,网络消费市场得到了较快的发展。同时,由于居民社会阶层的差别及相应居住空间的分异,导致网络消费呈现出不同的空间特征,并且对实体空间产生不同程度的影响。通过对南京居民网络消费情况的问卷调查,分析个人社会经济属性、住房状况、交通方式以及移动网络使用等体现居民社会阶层状况的要素对居民网络消费选择的影响,进而分析居民网络消费的空间分布特征,研究城市不同空间的网络消费构成和网络消费商品类型特征,在此基础上分析不同空间网络消费对交通出行和实体商业空间结构的影响程度。     

梅雨锋云系的模态研究Ⅱ：出现频率及转换特点

基于经验正交函数（EOF）分解方法提取的1998～2008年梅雨活跃期共16次过程的梅雨锋云系主导模态,讨论了这些主导模态的发生频率、维持时间及相互转换特征。结果表明,在16个梅雨活跃期内,云系的第1主导模态正相位出现的频率最高,并且在4个活跃期内作为首次出现模态率先出现;第5模态正位相的出现频率最低,且该模态多数伴随其他模态一同出现,为过渡模态。统计发现,各主导模态的维持时间并不相同,其中第2模态负位相持续时间最长,为81 h;第5模态正位相的最大持续时间最短,仅为18 h;第6模态负位相的平均持续时间最长。对不同主导模态转换方向和频次的计算表明,各个位相的模态最多（最少）向9种（3种）（含正负位相）其他模态进行转换。除第6模态负位相和第5模态负位相向其他模态的转化具有较好的方向性外,其他模态间的相互转换多表现出随机性特征。其中,第6模态负位相在超过半数的情况下向第5模态负位相转换,而第5模态负位相则多向第3模态正位相和第1模态负位相进行转换。     

Evaluation of the biological toxicity of fluorine in Antarctic krill

Antarctic krill is a potentially nutritious food source for humans, but fluorine(F) toxicity is a matter of concern. To evaluate the toxicity of F in Antarctic krill, 30 Wistar rats were divided into three groups with different dietary regimens: a control group, a krill treatment group(150 mg·kg-1 F), and a sodium fluoride(NaF) treatment group(150 mg·kg-1 F). After three months, F concentrations in feces, plasma, and bone were determined, and the degree of dental and skeletal fluorosis was assessed. The F concentrations in plasma and bone from the krill treatment group were 0.167 0±0.020 4 mg.L-1 and 2 709.8±301.9 mg·kg-1, respectively, compared with 0.043 8±0.005 5 mg·L-1 and 442.4±60.7 mg·kg-1, respectively, in samples from the control group. Concentrations of F in plasma and bone in the krill treatment group were higher than in the control group, but lower than in the NaF treatment group. The degree of dental fluorosis in the krill treatment group was moderate, compared with severe in the NaF treatment group and normal in the control group. The degree of skeletal fluorosis did not change significantly in any group. These results showed that the toxicity of F in Antarctic krill was lower than for an equivalent concentration of F in NaF, but it was toxic for rats consuming krill in large quantities. To conclude, we discuss possible reasons for the reduced toxicity of F in Antarctic krill. The present study provides a direct toxicological reference for the consideration of Antarctic krill for human consumption.     

广西寒露风开始期短期气候预测方法研究

利用1951-2013年广西90个气象观测站气温资料、国家气候中心74项指数和美国National Oceanic and Atmospheric Administration（NOAA）的Climate Prediction Center（CPC）60项指数以及海温和陆地雪盖资料、美国国家冰雪研究中心（NSIDC）的两极海冰资料,使用相关分析方法得到广西寒露风开始期气候影响因子,利用逐步回归和神经网络方法进行寒露风开始期的预测。结果表明：寒露风开始期与前一年9-10月北极海冰面积、当年3月南极海冰面积、前一年6月欧亚雪盖、当年5月北美雪盖、北半球雪盖的相关显著。与前一年9月北半球极涡面积指数、前一年10月亚洲区极涡面积指数、前一年3月热带印度洋海温偶极子等指数相关显著。粒子群-神经网络方法预测误差低于逐步回归方法,预报能力有明显提高。     

Spatial distribution pattern of seafloor hydrothermal vents to the southeastern Kueishan Tao offshore Taiwan Island

Investigations of the diffusion activities both within and outside the seafloor hydrothermal vents, as well as related mineral genesis, have been one of the key focuses of ocean biogeochemistry studies. Many hydrothermal vents are distributed close to the southern Okinawa Trough on the less-than-30-m deep shallow seafloor off Kueishan Tao, northeast of Taiwan Island. Investigations of temperature, pH and Eh at four depths of hydrothermal plume were carried out near Kueishan Tao at the white(24.83°N, 121.96°E) and yellow(24.83°N, 121.96°E) vents. An 87 h of temperature time series observation-undertaken near the white vent showed that tide is the main factor affecting the background environment. Based on the observed data, 3-dimensional sliced diffusion fields were obtained and analyzed. It was concluded that the plume diffused mainly from north to south due to ebb tide. The yellow vent's plume could effect as far as the white vent surface. From the temperature diffusion field, the vortices of the plume were observed. The Eh negative abnormality was a better indicator to search for hydrothermal plumes and locate hydrothermal vents than high temperature and low pH abnormalities.     

东海西部陆架海域水团的季节特征分析

On the basis of the CTD data and the modeling results in the winter and summer of 2009, the seasonal characteristics of the water masses in the western East China Sea shelf area were analyzed using a cluster analysis method. The results show that the distributions and temperature-salinity characteristics of the water masses in the study area are of distinct seasonal difference. In the western East China Sea shelf area, there are three water masses during winter, i.e., continental coastal water（CCW）, Taiwan Warm Current surface water（TWCSW） and Yellow Sea mixing water（YSMW）, but four ones during summer, i.e., the CCW, the TWCSW, Taiwan Warm Current deep water（TWCDW） and the YSMW. Of all, the CCW, the TWCSW and the TWCDW are all dominant water masses. The CCW, primarily characterized by a low salinity, has lower temperature, higher salinity and smaller spatial extent in winter than in summer. The TWCSW is warmer, fresher and smaller in summer than in winter, and it originates mostly from the Kuroshio surface water（KSW） northeast of Taiwan, China and less from the Taiwan Strait water during winter, but it consists of the strait water and the KSW during summer. The TWCDW is characterized by a low temperature and a high salinity, and originates completely in the Kuroshio subsurface water northeast of Taiwan.     

鲜浒苔稀硫酸水解产糖工艺研究

分别用质量分数为0.6%、1.0%、1.4%、1.8%、2.2%的稀硫酸在121℃高温高压的条件下水解鲜浒苔30、60、90 min,浒苔水解残留物再用纤维素酶酶解,用DNS法测定上述各步骤中的还原糖产量。结果表明,浒苔总糖含量为67.2%。稀硫酸水解工艺中,硫酸质量分数为1.8%、水解90 min,浒苔还原糖转化率最高,达59.9%,每克干质量浒苔可产生447.0 mg还原糖。稀硫酸水解残留物纤维素酶酶解工艺中,浒苔还原糖转化率较低,在硫酸质量分数为0.6%、水解时间为60 min时还原糖转化率出现最大值,仅为6.5%。结果显示浒苔稀硫酸水解产还原糖效果显著,但并不能促进纤维素酶酶解。浒苔糖化可单独采用稀硫酸水解工艺,本实验条件下的最佳工艺条件为1.8%硫酸水解90 min。本研究探索了浒苔糖化工艺条件,对后续的浒苔燃料乙醇发酵研究具有一定的参考意义。     

洋山深水港区海域秋、冬季沉积物中重金属来源分析及生态风险评价(英文)

通过测定洋山深水港区海域2010~2013年6个航次秋、冬季沉积物中重金属含量,分析其时空差异,结果表明该海域沉积物重金属总体含量较低,空间分布较为均匀,Hg、Zn、Pb、Cd含量秋季明显高于冬季,As、Cu含量的季节性波动较小,用因子分析法研究该海域重金属来源,发现陆源工业、船舶航运排污以及有机质降解是研究海域Pb、Cd、Zn的主要来源;农业污染、码头货物装卸残留及建筑垃圾支配着Cu、As、Hg的来源。SQG-Q生态风险评价表明研究海域沉积物中重金属均存在中、低度的生态风险,秋季的生态风险高于冬季,Hg和Cu是主要生态风险因子;地累积指数法评价结果显示研究海域基本不受Hg、As、Zn、Pb的污染;Cd、Cu以轻度污染为主,部分站位属于偏中度污染,6种重金属污染程度依次为:CdCuPbZnHgAs。因子综合得分评价表明,重金属污染相对严重的站位依次为5号、4号及3号站位。但总体看来洋山深水港区附近海域沉积物中重金属污染状况属于轻度污染。