乳糖诱导重组别藻蓝蛋白基因在大肠杆菌中的表达

以重组别藻蓝蛋白表达工程菌JM109（DE3）／pET28-APC作为研究对象，对于JM109（DE3）菌株在乳糖诱导下表达重组蛋白的规律进行了研究。比较分析了不同生长阶段进行诱导，最佳乳糖浓度、诱导持续时间和诱导温度等参数对重组蛋白表达的影响。实验结果表明，采用JM109（DE3）为宿主菌，经过条件优化，乳糖诱导重组蛋白的表达量可以达到IPTG诱导的水平；较低的诱导培养温度能有效提高可溶性重组蛋白的表达。最后在摇瓶发酵结果的基础上，实现了乳糖诱导工程菌的5L全自动发酵罐培养。研究结果为在大肠杆菌大规模发酵中，运用廉价、无毒的乳糖作为诱导剂提供了一定的借鉴。     

悬浮液进样氢化物发生原子荧光法测定海洋沉积物样品中痕量砷

人类对砷的认识已有很长的历史,近几十年来关于砷对环境的污染以及对人体健康的影响日益受到人们的关注.砷是海洋沉积物中常见的主要环境污染元素之一,目前测定它的常用方法有砷钼酸-结晶紫分光光度法[1]、氢化物-原子吸收分光光度法[1]、催化极谱法[1]、氢化物-原子荧光光谱法[2],但是这些方法需将样品制备成溶液,处理过程冗长,易引起操作沾污和待测成分损失,而且试剂消耗量大,同时产生的酸气对操作人员的身体健康非常有害,为此分析工作者一直在寻求一些新的前处理技术.     

流动注射-氢化物发生原子荧光法测定海洋沉积物样品中痕量铅

铅是一种具有累积性的有害元素,是海洋监测调查中沉积物介质的必测元素之一,常用测定方法有双硫腙分光光度法、火焰原子吸收光谱法、无火焰原子吸收光谱法等[1],前两种方法灵敏度较低,测定时一般需要预富集,操作较繁琐;后一种方法虽然灵敏度高,但仪器昂贵,对操作人员要求高.氢化物发生原子荧光法由于灵敏度高、检出限低且线性范围宽,在铅的分析中日益被重视.     

A three-dimensional model study on ocean dynamic response to traveling cyclone over the Huanghai Sea

On the basis of numerical simulation of the mean circulation and relevant thermal-salinity fields in June with a three-dimensional ocean model (ECOM-si), the model outputs are used as first guess of initial fields for numerical integration of the model equations and the numerical results are applied to investigating the dynamical responses of the Huanghai Sea and the East China Sea (HECS) in the course of a weak land-to-sea cyclone‘s passage over the Huanghai Sea on 15-16 June 1999. Predominance of the dynamic impact of cyclone over the thermal one in June in the HECS is justified using observations and model simulations.The cyclone and its surrounding weather system, i.e,, subtropical high ridge to its south could influence current and thermal fields in the Bohai Sea, the Huanghai Sea and the northern East China Sea even though the intensity of cyclone was rather weak. The response of oceanic currents to the wind stresses driven by the cyclone and its southern subtropical high were strongly characterized by the wind drift with its extent of equivalent scale of cyclone in the horizontal and of Ekman layer in the vertical. The sea response at a given site was closely related to the transient local wind speed and direction,especially was sensitive to the local wind direction,which is demonstrated at three points locating at the southern and western Huanghai Sea and the northern East China Sea. So the sea responses at locations differed considerably from one another. Current responded to the wind stress in a simple way:directly to the wind-driven current and subsequent gradient current and slope current, etc., whereas sea temperature responded to the wind stress in two ways: directly to the cyclone-induced cooling and indirectly to water movements both in the horizontal and the vertical by the cyclone‘ s wind stress. So the sea temperature variation under the influence of cyclone was more complicate than the current. The HECS in response to the cyclone and its ambient weather system was likely to be a fast process and such a response could last at least for more than 1d. Current increased with the duration of wind stress exerted on the surface and decreased with the increasing depth. Affected by the cyclone, the maximum sea surface temperature decreased by almost 1.6℃ during the 24h cyclone.     

雷州半岛灯楼角热带海岸的景观生态分类

海岸景观由于其边缘效应而具有较高的生产力和生物多样性水平,尤其是本区的热带海岸景观,由于发育有我国大陆唯一的珊瑚礁而显独特。本文根据景观生态学的分类方法,将灯楼角热带海岸景观生态划分为3大类（自然景观、经营景观和人工景观）24亚类,并揭示了本区的景观生态系统的等级和景观的演变与时空分布;由陆向海方向上依次出现陆地景观、海岸景观和沿岸浅海景观,而区域自然景观的出现时间序列上,则是陆地景观、沿岸线海景观和海岸景观。     

三种海产帘蛤目贝类的核型研究

以大竹蛏、紫石房蛤和中国蛤蜊的鳃组织为材料,经注射植物血球凝集素和秋水仙素处理,低渗作用后,采用滴片法和压片法制片,对3种贝的核型进行了研究.结果表明,3种贝类的二倍体染色体数均为38条,大竹蛏的核型为26m+6sm+2st+4t,NF=0;紫石房蛤的核型为32m+2sm+4st/t,NF=2;中国蛤蜊的核型为20m+16sm+2st/t,NF=4.在3种贝类的染色体中均未发现随体及性染色体.     

日本鬼鲉蜇伤与P物质和生长抑素的关系及其粗毒对免疫细胞活性影响

１９９７年６月在山东省日照市岚山头附近海域收集日本鬼ｙｏｕ,采用放射凤学方法检测了注射毒腺粗提物大鼠局部肌肉组织、血浆、疹髓、延髓、中脑、下丘脑及免疫器官内Ｐ物质和生长抑素含量变化;用ＭＴＴ法检测了朱粗提物在整体和离体条件下对免疫细胞活性的影响;用生化方法检测了毒腺组织中透明质酸酶活性。结果表明,与对照组大鼠相比,实验组大鼠局部肌肉及延髓、中脑、胸腺中脾脏中Ｐ物质含量增加,而下丘脑中Ｐ物质含量下降     

渤海湾表层沉积物重金属与污染评价

用原子吸收法测定了渤海湾表层沉积物中重金属的含量,同时用2种评价方法对该海域底质环境进行了评价。结果表明:沉积物中汞、锌含量平均值超过标准,其余项目未发现超标;其分布特征由于受沿岸不同污染源的污染影响,重金属污染物难以形成一定规律的分布状态。同时该海域底质环境质量总指数已达到污染程度和很强的生态危害,其中主要污染元素是Hg,已达重污染,并形成极强的生态危害。     

扬子板块西缘石棉安顺场新元古代钾长花岗岩地球化学特征及其地质意义

扬子板块西缘新元古代岩浆活动强烈,其成因研究对探讨Rodinia超大陆的演化有着重要意义。本文对石棉安顺场钾长花岗岩形成年龄及其地球化学特征研究,结果表明锆石~(206)Pb/~(238)U年龄加权平均值为777.3±4.8 Ma(MSWD=0.23,2σ),代表花岗岩的结晶年龄。岩石SiO_2含量高(72.64%~76.27%),铝饱和指数A/CNK大于1(1.06~1.24),K_2O/Na_2O在1.40~2.22之间,里特曼指数σ小于3.3(2.08~2.74)。岩石轻稀土元素(LREE)富集,Nb、Ta轻微亏损,Eu负异常明显(δEu=018~0.23)。岩石的ε_(Nd)(t)为0.5~3.3(平均值为2.1),T_(DM)在1.19~1.61Ga之间,反映其源区以古老地壳物质为主。铅的初始同位素比值分别为[n(~(206)Pb)/n(~(204)Pb)]i=15.4103~17.2707,[n(~(207)Pb)/n(~(204)Pb)]i=15.4265~15.5479,[n(~(208)Pb)/n(~(204)Pb)]i=33.3518~35.8641。此外,岩石具有高的Rb/Sr比值和低的Ca O/Na2O比值、较低的Al_2O_3/TiO_2比值和低的Rb/Ba比值,表明其起源于泥质岩石的部分熔融。综合地球化学、同位素特征和区域地质资料,我们认为石棉安顺场钾长花岗岩为过铝质高钾钙碱性S型花岗岩,它是地壳泥质源岩部分熔融的产物,形成于挤压的构造环境中。     

图像数据挖掘软件原型系统的设计与开发

提出了图像数据挖掘软件原型系统的设计与开发的试验框架,阐述了其设计与开发方法,介绍了各个模块的功能、设计与开发情况。