Tidal Freshwater Marshes Harbor Phylogenetically Unique Clades of Sulfate Reducers That Are Resistant to Climate-Change-Induced Salinity Intrusion

Rates of sea level rise associated with climate change are predicted to increase in the future, potentially altering ecosystems at all ecological levels. Sea level rise can increase the extent of brackish water intrusion into freshwater ecosystems, which in turn can affect the structure and function of resident microbial communities. In this study, we performed a year-long mesocosm experiment using intact tidal freshwater marsh sediment cores to examine the effect of a 5-part per thousand (ppt) salinity increase on the diversity and community composition of sulfate-reducing prokaryotes. We used a clone library approach to examine the dsrA gene, which encodes an important catalytic enzyme in sulfate reduction. Our results indicate that tidal freshwater marshes contain extremely diverse communities of sulfate-reducing bacteria. Members of these communities were, on average, only 71 % similar to known cultured sulfate reducers and 81 % similar to previously sequenced environmental clones. Salinity and associated increases in sulfate availability did not significantly affect the diversity or community composition of sulfate-reducing prokaryotes. However, carbon quality and quantity, which correlated with depth, were found to be the strongest drivers of sulfate-reducing community structure. Our study demonstrates that the sulfate-reducing community in tidal freshwater marsh sediments appears resistant to increased salinity in the face of sea level rise. Additionally, the microorganisms that comprise this sulfate-reducing community appear to be unique to tidal freshwater marsh sediments and may represent novel lineages of previously undescribed sulfate reducers.     

Response scenarios for the deltaic plain of the Rhône in the face of an acceleration in the rate of sea-level rise with special attention to<Emphasis Type="Italic">Salicornia</Emphasis>-type environments

One of the most critical problems facing many deltaic wetlands is a high rate of relative sea-level rise due to a combination of eustatic sea-level rise and local subsidence. Within the Rhône delta, the main source of mineral input to soil formation is from the river, due to the low tidal range and the presence of a continuous sea wall. We carried out field and modeling studies to assess the present environmental status and future conditions of the more stressed sites, i.e.,Salicornia-type marshes with a shallow, hypersaline groundwater. The impacts of management practices are considered by comparing impounded areas with riverine areas connected to the Rhône River. Analysis of vegetation transects showed differences between mean soil elevation ofArthrocnemum fruticosum (+31.2 cm),Arthrocnemum glaucum (+26.5 cm), bare soil (+16.2 cm), and permanently flooded soil (?12.4 cm). Aboveground and belowground production showed that root:shoot ratio forA. fruticosum andA. glaucum was 2.9 and 1.1, respectively, indicating more stressful environmental conditions forA. glaucum with a higher soil salinity and lack of soil drainage. The annual leaf litter production rate of the two species is 30 times higher than annual stem litter production, but with a higher long-term decomposition rate associated with leaves. We developed a wetland elevation model designed to predict the effect of increasing rates of sea-level rise on wetland elevation andSalicornia production. The model takes into account feedback mechanisms between soil elevation and river mineral input, and primary production. In marshes still connected to the river, mineral input decreased quickly when elevation was over 21 cm. Under current sea-level rise conditions, the annual amount of riverine mineral input needed to maintain the elevation of the study marshes is between 3,000 and 5,000 g m^?2 yr^?1. Simulations showed that under the Intergovernmental Panel on Climate Change best estimate sea-level rise scenario, a mineral input of 6,040 g m^?2 yr^?1 is needed to maintain marsh elevation. The medium term response capacity of the Rhône deltaic plain with rising sea level depends mainly on the possibility of supplying sediment from the river to the delta, even though the Rhône Delta front is wave dominated. Within coastal impounded marshes, isolated from the river, the sediment supply is very low (10 to 50 g m^?2 yr^?1), and an increase of sea-level rise would increase the flooding duration and dramatically reduce vegetation biomass. New wetland management options involving river input are discussed for a long-term sustainability of low coastal Mediterranean wetlands.     

Use of Fossil Bryozoans in Sourcing Lithic Artifacts

This study reviews the occurrence and potential of bryozoans within lithic artifacts and also sets out a methodology for their use in sourcing and discusses the advantages and disadvantages of this approach. We present case studies from our own research and from the literature on using bryozoans in sourcing archaeological lithic artifacts. Fossil bryozoans of different ages and clades can be effectively used to determine the material source of lithic artifacts from a variety of prehistoric ages. The case studies included in this report span the stratigraphic range of bryozoans from the Ordovician to the Neogene. The bryozoans came from four different orders: trepostome, fenestrate, cyclostome, and cheilostome. The use of these lithic artifacts ranged back to 25 ka. Although the majority of the fossil bryozoans were incidental in the artifacts, the bryozoans were still useful for determining their original source rock. The improved searchable online paleontologic databases allow for more efficient use of fossil bryozoans to constrain the stratigraphic and paleogeographic distribution of source outcrops. Although generally underutilized in sourcing prehistoric lithic artifacts, it is clear that by analyzing bryozoans, an increased understanding of the lithologic nature of these materials could be gained by the archaeological community.     

Salting-out of methane in single-salt solutions at 25°C and below 800 psia

Aqueous solubilities of methane at 25°C have been determined in single-salt solutions equilibrated with a CH₄ gas phase at 350, 550, and 750 psia. Measurements were made over a range of ionic strengths in NaCl, KCl, CaCl₂, MgCl₂, Na₂SO₄, K₂SO₄, MgSO₄, Na₂CO₃, K₂CO₃, NaHCO₃, and KHCO₃ aqueous solutions.At 25°C and constant pressure and methane fugacity, methane solubilities were largely controlled by the stoichiometric ionic strength, I, and the cation of the salt. Except for an increased salting-out due to HCO₃^?, the anion effect was relatively insignificant. Different but consistent solubility trends were followed in monovalent and divalent cation salt solutions as a function of I. Solubilities increased in salt solutions having a common anion in the following cation sequence: Na⁺ < K⁺ ? Ca²⁺ < Mg²⁺.The molal salting coefficient, k_m, for each salt was constant under the experimental conditions of the study, k_m is defined by $\text{log}\text{γ}\text{ch}{}_4\text{I}$ where $\text{γ}\text{ch}{}_4$, the molal activity coefficient, is the methane solubility ratio () measured at constant temperature, pressure, and CH₄ fugacity. Single-salt k_m values are as follows: 0.121, NaCl (4m); 0.121, Na₂SO₄ (1m); 0.118, Na₂CO₃ (1.5m); 0.146, NaHCO₃ (0.5m); 0.101, KCl (4m); 0.108, K₂SO₄ (0.5m); 0.111, K₂CO₃ (2m); 0.145, KHCO₃ (0.5m); 0.071, CaCl₂ (2m); 0.063, MgCl₂ (2m); and 0.066, MgSO₄ (1.5m) where the molalities in parentheses refer to the maximum salt concentrations used in this study.     

Late Holocene Marsh Expansion in Southern San Francisco Bay, California

Currently, the largest tidal wetlands restoration project on the US Pacific Coast is being planned and implemented in southern San Francisco Bay; however, knowledge of baseline conditions of salt marsh extent in the region prior to European settlement is limited. Here, analysis of 24 sediment cores collected from ten intact southern San Francisco Bay tidal marshes were used to reconstruct spatio-temporal patterns of marsh expansion to provide historic context for current restoration efforts. A process-based marsh elevation simulation model was used to identify interactions between sediment supply, sea-level rise, and marsh formation rates. A distinct age gradient was found: expansion of marshes in the central portion of southern San Francisco Bay dated to 500 to 1500 calendar years before present, while expansion of marshes in southernmost San Francisco Bay dated to 200 to 700 calendar years before present. Thus, much of the tidal marsh area mapped by US Coast Survey during the 1853–1857 period were in fact not primeval tidal marshes that had persisted for millennia but were recently formed landscapes. Marsh expansion increased during the Little Ice Age, when freshwater inflow and sediment influx were higher than during the previous millennium, and also during settlement, when land use changes, such as introduction of livestock, increased watershed erosion, and sediment delivery.     

Tertiary stratigraphy of Western Australia

This paper is a summary of the present knowledge of the Tertiary stratigraphy of Western Australia. Also included is new information on the Cainozoic of the Carnarvon Basin, a result of petroleum exploration in the area.

Tertiary rocks formed during more than one cycle of deposition in three basins (Eucla, Perth, and Carnarvon), and also as thin units deposited in a single transgression along the south coast. The Tertiary stratigraphy of the Bonaparte Gulf Basin is not well known.

Drilling in the Eucla Basin has encountered up to 400 m of Tertiary in the south central part, with uniform thinning towards the margins. The section begins with a middle‐upper Eocene carbonate unit which represents the dominant event in the Tertiary sedimentation in this basin. More carbonates were deposited in the late Oligocene‐early Miocene and middle Miocene.

Along the south coast, the so‐called Bremer Basin, the Plantagenet Group (up to 100 m) of siltstone, sandstone, spongolite, and minor limestone, was deposited during the late Eocene.

The Perth Basin contains up to 700 m of Tertiary sediment, formed during at least two phases of sedimentation. The upper Paleocene‐lower Eocene Kings Park Formation consists of marine shale, sandstone, and minor limestone, with a thickness of up to 450 m. The Stark Bay Formation (200 m) includes limestone, dolomite, and chert formed during the early and middle Miocene. Events after deposition of the Stark Bay Formation are not well known.

The northern Carnarvon Basin and Northwest Shelf contain by far the most voluminous Tertiary sediment known from Western Australia: 3500 m is known from BOCAL's Scott Reef No. 1. A more usual maximum thickness is 2500 m. Most sediments were laid down in four episodes, separated by unconformities: late Paleocene‐early Eocene; middle‐late Eocene; late Oligocene‐middle Miocene; and late Miocene to Recent.

The Paleocene‐early Eocene cycle consists of about 100–200 m (up to 450 m in the north) of carbonate, shale, and marl of the Cardabia Group containing rich faunas of planktonic foraminifera.

The middle‐late Eocene sediments include diverse rock types. Marine and nonmarine sandstone formed in the Merlinleigh Trough. At the same time, the Giralia Calcarenite (fauna dominated by the large foraminifer Discocyclina) and unnamed, deeper water shale, marl, and carbonate (with rich planktonic foraminiferal faunas) formed in the ocean outside the embayment. Thickness is usually of the order of 100–200 m.

The main cycle of sedimentation is the late Oligocene‐middle Miocene, during which time the Cape Range Group of carbonates formed. This contains dominantly large foraminiferal faunas, of a wide variety of shallow‐water microfacies, but recent oil exploration farther offshore has recovered outer continental shelf facies with abundant planktonic foraminifera. A minor disconformity representing N7 and perhaps parts of N6 and N8 is now thought to be widespread within the Cape Range Group. The last part of this cycle resulted in sedimentation mainly of coarse calcareous marine sandstone (unnamed), and, in the Cape Range area, of the sandstone and calcareous conglomerate of the Pilgramunna Formation. Maximum thickness encountered in WAPET wells is 900 m.

After an unconformity representing almost all the late Miocene, sedimentation began again, forming an upper Miocene‐Recent carbonate unit which includes some excellent planktonic faunas. Thickness is up to 1100 m.

Thin marine sediments of the White Mountain Formation outcrop in the Bonaparte Gulf Basin. They contain some foraminifera and a Miocene age has been suggested.     

Late stage bivalve larvae in a well-mixed estuary are not inert particles

Depth-specific sampling at a single location was used to examine the vertical distribution of pediveliger larvae of bivalve mollusks in the York River, an estuary of Chesapeake Bay, Virginia. The water column at the sampling site was usually well mixed, lacking consistent temperature or salinity gradients for larvae. Four species showed strong vertical stratification when collected simultaneously at three depths. Pediveliger stage larvae of a clam (Cyrtopleura costata), an oyster (Crassostrea virginica), and a shipworm (Bankia gouldi) were most abundant near the benthos, and least abundant near the surface. A mussel (Geukensia demissa), showed the reverse trend, with most pediveliger larvae near the surface. Tidal stage had a slight effect on two species (C. costata andC. virginica), but only to increase the relative abundance of larvae near the benthos during flood tide. Otherwise, neither tidal phase nor light levels (night versus day) had detectable effects on distribution patterns. Sampling very close to the sediment-water interface provided no evidence that pediveliger larvae spent a significant proportion of their time in this location. While the behavior of precompetent bivalve larvae may tend to retain them within an estuary, that of competent-to-metamorphose pediveliger larvae does not appear to have that function. Pediveliger larvae may regulate their depth to best locate potential settlement substrate. Such an hypothesis is consistent with adult habitat zones of at least some of these species in Chesapeake Bay. Removing competent-to-settle larvae from analyses of larval distributions in estuaries will enhance or clarify depth distribution patterns observed for earlier-stage larvae.     

Development of ecosystem indicators for the Suwannee River estuary: Oyster reef habitat quality along a salinity gradient

The Suwannee River watershed is one of the least developed in the eastern United States, but with increasing urbanization it is facing potential long-term alterations in freshwater flow to its estuary in the Gulf of Mexico. The purpose of this study was to develop biological indicators of oyster reef state along a natural salinity gradient in the Suwannee River estuary in order to allow the rapid assessment of the effect of changing freshwater input to this system. Percent cover and density of three size classes of living oysters, as well as the abundance of several predominant reef-associated invertebrates, were measured along a broad salinity gradient in the estuary and were correlated with salinity estimates from a long-term database for the preceding 12–24 mo. All eastern oyster,Crassostrea virginica, parameters (percent cover and density of three size classes) were significantly and negatively related to salinity. Data from samples collected near the lower intertidal were more closely dependent upon salinity than were samples from the higher intertidal at the same sites. Salinity differences were most closely reflected in differences in total oyster cover. This relationship corresponded with a general decline in oyster habitat with increasing distance from the mouth of the Suwannee River. Species richness was significantly and positively correlated with allC. virginica parameters (percent cover and density of three size classes), but the relationship explained only about half the variability. Density data of the hooked mussel,Ischadium recurvum, and a mud crab,Eurypanopeus depressus, were positively and strongly correlated withC. virginica parameters, likely reflecting the abundance of habitat provided byC. virginica shells. All of the biological indicators measured responded similarly along the salinity gradient, indicating they provide reliable indices of the effect of changing salinities in the Suwannee River estuary over the previous 1 or 2 yr. Some areas of positive relief defined as reefs 30 years ago are no longer oyster habitat, suggesting an ongoing decline, but nearshoreC. virginica were abundant. *** DIRECT SUPPORT *** A02BY003 00002