The Development of the Chagas' Online Data Entry System (CODES-GIS)

This article describes a web‐based data entry and GIS‐driven mapping system designed for an ethnographic and entomological survey of Chagas’ disease, an emerging zoonotic disease, and Triatoma dimidiata, a primary vector, in the Los Tuxtlas region of Veracruz, Mexico. To better understand this disease in the region, a collaborative, multi‐disciplinary study was initiated to conduct a spatial investigation of T. dimidiata and a community‐by‐community survey of local perceptions of the disease. In order to facilitate such a collaborative effort the CODES‐GIS was developed. This system allows for (near) real‐time mapping, analyses, disease reporting, and results sharing. CODES‐GIS provides a framework for a research team working in a remote area with limited technology, software, or GIS expertise to benefit from (near) real‐time spatial analyses performed at collaborating institutions. The system is bi‐directional, where field personnel can upload data to the system for field‐based map production. Likewise, laboratory personnel can upload diagnostics data for viewing by field personnel. In this way, the system provides a virtual link between the field and the laboratory to increase the speed at which results are returned to the local community. The CODES‐GIS is described along with a selection of study results.     

Appendicularian assemblages and their space–time variability off the La Plata River,SW Atlantic Ocean

Among tunicates, the small planktonic appendicularians generally comprise a significant fraction of the mesozooplankton of estuarine environments. However, no spatio-temporal studies nor estimation of some ecological parameters such as biomass or pellet production of these organisms have been previously reported in La Plata River estuary. We studied the species composition and abundance as well as biomass and pellet production, associated with the river plume variations using data collected during seven spring (October) cruises. Approximately 80 samples were collected in each cruise with a Pairovet net (200 μm mesh size) trawled vertically through the water column; CTD data profiles were also recorded. Three transects at the north, middle and south of the estuary were performed in order to compare the environmental gradients to the species distribution. The river's discharges varied between years and it was reflected on the salinity distribution. The salinity was the main factor driving species composition. Oikopleura dioica, Oikopleura fusiformis and Fritillaria borealis were found, O. dioica being the dominant one. We detected that O. fusiformis was more abundant in stratified waters between salinities of 31 and 33 mainly during 1994, 1995 and 1996. Fritillaria borealis was found only in salinities higher than 33 and in homogenous waters being more abundant in 1995. This year was singular in the sense that no river plume was observed at the middle transect, as it is shown by the homogenous salinity profiles. Oikopleura dioica was present in stratified waters at all salinity ranges. In 1993, it was more abundant at low salinities with temperatures between 12 and 16 °C and a predominance of juvenile animals was observed. The contribution in biomass and pellet production of O. dioica in the stratified waters of this front was very high (27.91–66.58 g C m⁻² d⁻¹) revealing their importance in the estuary's carbon cycle.     

Land use and the spatial distribution of perfluoroalkyl compounds as measured in the plasma of bottlenose dolphins (Tursiops truncatus)

The distribution of perfluoroalkyl compounds (PFCs) in the environment is well documented with higher concentrations observed in wildlife located in industrial and urban areas. This study examined the distribution of PFCs in relation to land use using blood samples collected from bottlenose dolphins during capture-release health assessment surveys conducted in Charleston, SC. The study area was partitioned into three subareas (ACW, CHS, and SRE) based upon habitat and land use characteristics. The ACW and CHS subareas are characterized by high degrees of industrial and urban land uses, while the SRE subarea is more residential and characterized by a lower degree of developed land use. Long-term monitoring data from photo-identification surveys were used to group bottlenose dolphins based on their proportions of sightings in the different subareas. Dolphins affiliated with both the ACW and CHS subareas were observed to have significantly higher mean plasma concentrations of perfluorooctane sulfonate (PFOS), perfluorodecanoic acid (PFDA) and perfluoroundecanoic acid (PFUnA) than those affiliated with the SRE subarea. Dolphins affiliated with the ACW subarea were found to have a significantly higher mean plasma concentration of PFUnA than those affiliated with the CHS subarea. Further examination of the distribution of the PFCs revealed positive correlations with developed land uses and negative correlations with wetland/marsh land cover. A positive correlation was also observed between PFUnA and agricultural land use. The variability and scale of the observed contaminant burdens have important implications for the conservation and management of living marine resources and illustrates the importance of long-term monitoring of free-ranging wildlife species.     

Primary production and implications for metabolic balance in Hawaiian lee eddies

Recent discrepancies between geochemical and biological approaches for determining whether ocean ecosystems are net heterotrophic or net autotrophic have led to uncertainty in the net metabolic state of open ocean ecosystems. Geochemical approaches indicate that the oceans are net positive autotrophic, but direct observations based on short-term incubation techniques suggest that the ocean is in a state of net heterotrophy. One hypothesis for the apparent discrepancy is that net autotrophic production occurs in aperiodic “bursts,” which are superimposed on a more constant background state of net heterotrophy. Mixing events, which introduce new nutrients to the surface ocean, provide one mechanism for fueling such aperiodic bursts of net production. In conjunction with the Eddy Flux (E-Flux) program in the lee of the Hawaiian Islands during winter 2004–2005, we examined the relationship between photosynthesis and irradiance (P vs. E) in surface waters inside and outside of two cold-core, cyclonic eddies, and conducted five incubation experiments to examine the metabolic response of mixed-layer plankton communities to nutrient-rich deep-sea water additions. Our results showed that in the mixed layer, maximum rates of light-saturated photosynthesis, derived from photosynthesis–irradiance experiments were not significantly different inside vs. outside the eddies (p=0.35 and 0.44 for E-Flux I and E-Flux III, respectively). Addition of nutrients to mixed-layer water showed that (1) gross primary production (GPP) became decoupled from a more constant rate of respiration and (2) net system metabolism shifted from approximate balance, or slight net heterotrophy, to a demonstrably net autotrophic system. From these results, we determined that the threshold GPP for net autotrophic production for the mixed layer of the study region was 1.65 mmol O₂ m⁻³ d⁻¹, which is consistent with previous estimates for the oligotrophic open ocean.     

Geometry and brittle deformation of the subducting Nazca Plate, Central Chile and Argentina

We use data from the Chile Argentina Geophysical Experiment (CHARGE) broad-band seismic deployment to refine past observations of the geometry and deformation within the subducting slab in the South American subduction zone between 30°S and 36°S. This region contains a zone of flat slab subduction where the subducting Nazca Plate flattens at a depth of ∼100 km and extends ∼300 km eastward before continuing its descent into the mantle. We use a grid-search multiple-event earthquake relocation technique to relocate 1098 events within the subducting slab and generate contours of the Wadati-Benioff zone. These contours reflect slab geometries from previous studies of intermediate-depth seismicity in this region with some small but important deviations. Our hypocentres indicate that the shallowest portion of the flat slab is associated with the inferred location of the subducting Juan Fernández Ridge at 31°S and that the slab deepens both to the south and the north of this region. We have also determined first motion focal mechanisms for ∼180 of the slab earthquakes. The subhorizontal T -axis solutions for these events are almost entirely consistent with a slab pull interpretation, especially when compared to our newly inferred slab geometry. Deviations of T -axes from the direction of slab dip may be explained with a gap within the subducting slab below 150 km in the vicinity of the transition from flat to normal subducting geometry around 33°S.