Effect of graded levels of dietary carbohydrate on growth,feed utilisation and intestinal microbial community structure in dusky kob Argyrosomus japonicus fed a pelleted diet

The ability to utilise carbohydrates is limited for many predatory marine fishes. Graded levels of dietary carbohydrate (4.1–24.6%) were formulated using pregelatinised maize starch, to determine optimal levels for dusky kob Argyrosomus japonicus, an emerging mariculture finfish for which pelleted feeds are being developed. Specific growth rate increased with an increase in the carbohydrate level up to 16.72%, after which it declined. Feed utilisation followed a similar trend, with the best feed conversion ratio (1.28) and protein efficiency ratio (1.76) recorded at 16.4% carbohydrate. Lipid vacuolisation of the hepatocytes was evident in all livers examined, with melano-macrophage aggregates in those of fish fed 24.6% carbohydrate suggesting starvation. Gut bacterial community profiles were variable but were not influenced by dietary carbohydrate level and differed mostly between fish fed trout feed prior to the trial and those fed experimental diets containing starch. The dusky kob were able to clear glucose from their blood when fed up to 16.4% carbohydrate, but glucose removal was not achieved at 24.6% carbohydrate. In conclusion, dusky kob has a limited ability to utilise cooked starch as a carbohydrate source, which may be included in pelleted feed at 16.4% without adverse effects. For this species, levels of dietary carbohydrate above this may result in symptoms consistent with physiological breakdown, including reduced growth, reduced feed intake and feed conversion efficiency, prolonged hyperglycaemia, liver pathology and altered microbial communities in the foregut.     

Behaviour of radiative accelerations in stars

Diffusion processes are able to stratify elements in stars on time-scales shorter than the evolution time on the main sequence. According to the efficiency of these diffusion processes, which depend on the radiative accelerations, abundances are time-dependent and inhomogeneous outside the stellar core. Large atomic and opacity data bases allow accurate computations of radiative accelerations, and several authors have developed efficient methods taking advantage of these large data bases. In the present work, these powerful tools are used to improve our knowledge on how radiative accelerations depend on the abundances of elements in stellar plasma.
In this study, we test the behaviours of radiative accelerations that are usually supposed. The results we obtain give some clues for future attempts to simplify the calculations of these accelerations.     

Evidence for transverse spread in Leonid meteors

We report here evidence for significant transverse spread of the light production region in bright Leonid meteors. One Leonid meteor has an apparent spread in the light production region of about 600 m perpendicular to the flight path for the meteor, that transverse spread persisting for at least 0.3 s. We have also detected short-duration, jet-like features emanating from a bright Leonid meteor recorded in 1998. These jet-like features have maximum spatial dimensions up to 1.9 km. While we cannot definitively rule out instrumental artefacts as a cause for these jet-like features, they may be evidence of motion contributing to the observed spatial spread in the light production region.     

A GIS‐based Approach in Support of an Assessment of Food Safety Risks

A Geographical Information System (GIS)‐based approach was developed for the identification of vulnerabilities and the measurement of risks associated with contamination of food systems with biological agents. In this research work, a tight integration of ArcGIS with the Arena simulation tool has been implemented. Arena was used to simulate and track contamination in a food distribution network and transmit the time dependent information to GIS. ArcGIS was employed to provide the primary user interface, process network data, and visualize the results. In addition, the GIS, through its powerful capabilities to process spatial data, could allow decision‐ makers to quickly determine the potential impact of a contamination event, at any stage, as a function of both time and geography. Two contamination scenarios along the farm‐to‐fork chain were examined to show the geographic zone and the proportion of the population affected by the contamination. A constraint Voronoi data structure was developed to define influence zones (these were color coded according to a dynamic risk index), to identify those areas that are at greatest immediate risk as time progresses, and to estimate the population affected by these contamination events. This approach thus appears to have general application to many GIS‐based risk assessment problems.     

Transient analysis of the source of water to wells: Cape Cod, Massachusetts

A transient flow modeling analysis for potential public-supply wells on western Cape Cod, Massachusetts, demonstrates the difference between transient and steady-state recharge areas can have important implications for wellhead protection. An example of a single pumping well illustrates that commonly used steady-state time-related capture areas do not represent the recharge area and travel times of water being pumped from the well until sufficient time has elapsed for steady-state flow conditions to be established. Until that time, transient recharge areas are needed to account for the portion of water discharging from the well that entered the aquifer before pumping started. An example of two pumping wells demonstrates the same area at the water table cannot supply water to more than one well under steady-state flow conditions. Transient recharge areas to multiple wells can overlap, however, until steady-state flow conditions are established. The same area can, therefore, be a source of water to more than one well during early pumping times, and the water pumped from a given well may derive from source areas, including contaminated areas, that do not lie within the well's steady-state recharge area.     

Synthetic shorelines in New Zealand? Quantification and characterisation of microplastic pollution on Canterbury's coastlines

Microplastics are persistent environmental contaminants found in marine environments worldwide. Microplastic particles isolated from coastlines in the Canterbury region of New Zealand were quantified and characterised. Sediment samples were collected from 10 locations representing exposed-beach, estuarine and harbour environments in both urban and non-urban settings. Particles were isolated from sediments using an NaCl density-separation procedure and quantified and characterised with a combination of optical/fluorescence imaging and micro-Raman spectroscopy. Microplastics were detected at eight out of 10 locations, at concentrations ranging from 0–45.4 particles kg^?1 of dry sediment. The majority of microplastics were identified as polystyrene (55%), polyethylene (21%) and polypropylene (11%). Microplastic concentrations in exposed-beach environments were significantly greater than in harbour and estuarine environments.     

Hillslope groundwater discharges provide localized stream ecosystem buffers from regional per- and polyfluoroalkyl substances contamination

Emerging groundwater contaminants such as per- and polyfluoroalkyl substances (PFAS) may impact surface-water quality and groundwater-dependent ecosystems of gaining streams. Although complex near-surface hydrogeology of stream corridors challenges sampling efforts, recent advances in heat tracing of discharge zones enable efficient and informed data collection. For this study, we used a combination of streambed temperature push-probe and thermal infrared methods to guide a discharge-zone-oriented sample collection along approximately 6 km of a coastal trout stream on Cape Cod, MA. Eight surface-water locations and discharging groundwater from 24 streambed and bank seepages were analysed for dissolved oxygen (DO), specific conductance, stable water isotopes, and a range of PFAS compounds, which are contaminants of emerging concern in aquatic environments. The results indicate a complex system of groundwater discharge source flowpaths, where the sum of concentrations of six PFAS compounds (corresponding to the U.S. Environmental Protection Agency third Unregulated Contaminant Monitoring Rule “UCMR 3”) showed a median concentration of 52 ± 331 (SD) ng/L with two higher outliers and three discharges with PFAS concentrations below the quantification limit. Higher PFAS concentration was related (− 0.66 Spearman rank, p < .001) to discharging groundwater that showed an evaporative signature (deuterium excess), indicating flow through at least one upgradient kettle lake. Therefore, more regional groundwater flowpaths originating from outside the local river corridor tended to show higher PFAS concentrations as evaluated at their respective discharge zones. Conversely, PFAS concentrations were typically low at discharges that did not indicate evaporation and were adjacent to steep hillslopes and, therefore, were classified as locally recharged groundwater. Previous research at this stream found that the native brook trout spawn at discharge points of groundwater recharged on local hillslopes, likely in response to generally higher levels of DO. Our study shows that by targeting high oxygen discharges the trout may thereby be avoiding emerging contaminants such as PFAS in groundwater recharged farther from the stream.     

UV line profiles as a probe for atomic diffusion theory in stellar atmospheres

In quiet stellar atmospheres that are stabilized, for example, by the presence of strong magnetic fields, a non-uniform distribution of chemical elements (abundance stratification) is predicted by the diffusion theory. Spectroscopic observations of magnetic Ap stars provide a strong support for the diffusion theory. Stratification predictions in the upper atmosphere, in particular, strongly depend on the magnetic field geometry. Optical lines used in stratification analysis of Ap stars do not probe the upper atmospheric layers and, hence, cannot provide reliable information on abundance gradients there. We show the expected effect of stratification on the profiles of strong lines in the UV region and how these lines may be used for constraining diffusion theory.