Paper II – Dirt,dates and DNA: OSL and radiocarbon chronologies of perennially frozen sediments in Siberia,and their implications for sedimentary ancient DNA studies

Arnold, L. J., Roberts, R. G., MacPhee, R. D. E., Haile, J. S., Brock, F., Möller, P., Froese, D. G., Tikhonov, A. N., Chivas, A. R., Gilbert, M. T. P. & Willerslev, E. 2010: Paper II – Dirt, dates and DNA: OSL and radiocarbon chronologies of perennially frozen sediments in Siberia, and their implications for sedimentary ancient DNA studies. Boreas, Vol. 40, pp. 417–445. 10.1111/j.1502‐3885.2010.00181.x. ISSN 0300‐9483 The sedimentary ancient DNA (sedaDNA) technique offers a potentially invaluable means of investigating species evolution and extinction dynamics in high‐latitude environments. An implicit assumption of the sedaDNA approach is that the extracted DNA is autochthonous with the host deposit and that it has not been physically transported from older source deposits or reworked within the sedimentary profile by postdepositional mixing. In this paper we investigate whether these fundamental conditions are upheld at seven perennially frozen wetland sites across the Taimyr Peninsula and coastal lowlands of north‐central Siberia. Optically stimulated luminescence (OSL) and radiocarbon (¹⁴C) dating are used to constrain the ages of both the inorganic and organic fractions of perennially frozen deposits from which sedaDNA of extinct and extant species have been recovered. OSL and ¹⁴C age/depth profiles, as well as single‐grain equivalent dose (D_e) distribution characteristics, are used to assess the stratigraphic integrity of these sedaDNA sequences by (i) identifying the presence of primary or reworked organic and inorganic material, and (ii) examining the types of depositional and postdepositional processes that have affected specific sedimentary facies. The results of this study demonstrate that even though DNA preservation and stratigraphic integrity are commonly superior in perennially frozen settings, this does not, in itself, guarantee the suitability of the sedaDNA approach. The combined OSL and ¹⁴C chronologies reveal that certain perennially frozen sites may be poorly suited for sedaDNA analysis, and that careful site selection is paramount to ensuring the accuracy of any sedaDNA study – particularly for ‘latest appearance date’ estimates of extinct taxa.     

CALCULATION OF SYNTHETIC REFLECTION SEISMOGRAMS IN THE FREQUENCY DOMAIN *

Calculation of reflection seismograms in the frequency domain makes it possible to treat absorption correctly, i.e. makes it possible to include dispersion (= causality) and frequency dependent reflection coefficients. In the paper it is shown how such a calculation can be done in a reasonably efficient manner.     

Holocene shore displacement and deglaciation chronology in Norrbotten, Sweden

The coastal zone of Norrbotten, northern Sweden, was gradually inundated by the Ancylus Lake following the retreating ice margin and forming a highest coastline approximately 210 m above the present sea level. The succeeding shore displacement is reconstructed based on lithological investigations and radiocarbon datings of identified isolation sequences from 12 cored lake basins. The highest lake basins, along with two basins above the highest shoreline, suggest ice-free conditions already at 10 500 cal. yr BP. This is at least 500 years earlier than previously thought and implies rapid ice-sheet break-up in the Gulf of Bothnia. The shore displacement (RSL) curve represents a forced regression of successively decreasing rate through the Holocene, from 9 m/100 yr to 0.8 m/100 yr. During the first 1000-1200 years, the isostatic uplift is exponentially declining, followed by a constant uplift rate from c. 9500 cal. yr BP to 5500-5000 cal. yr BP. The last 5000 years seem to be characterized by a low but constant rebound rate. The development of the Ancylus Lake stage of the Baltic may also be discerned in the Norrbotten RSL curve, suggesting that the chronology of the Ancylus Lake stages may have to be revised. The Littorina transgression is also reflected by the RSL curve shape. In addition, a series of early to mid-Holocene beach terraces were OSL-dated to allow for comparison with the ¹⁴C-dated shore displacement curve. Interpretations of these ages and their relation to former sea levels were clearly more problematic than the dating of the lake basin isolations.     

Salinity and redox alternations in the northwestern Baltic proper during the late Holocene

Selected geochemical parameters and siliceous microfossil assemblages in Baltic Sea sediments are presented which reflect past variations in redox conditions, salinity and primary production. The sediments were deposited during the freshwater Ancylus Lake (9500-8000 ¹⁴C BP) and brackish Litorina Sea (8000-3000 BP) stages of the Baltic. The diatom record shows that surface-water salinity increased further at c . 7000-6500 BP, although smaller amounts of brackish water entered the basin from c . 8000 BP onwards. Attempts to use exchangeable Mg as a palaeosalinity indictor were not applicable. Gross primary productivity increased along with salinity, which has been interpreted as an effect of nutrient enrichment in the photic zone. This led between c . 6500 and 4500 BP to a high accumulation of organic carbon, anoxic or nearly anoxic bottom conditions and formation of laminated deposits. Certain laminae consist of alternating layers of organic and minerogenic material and were probably formed annually, i.e. in the manner of varves. The laminated successions are distinguished by enrichments of V, Cu and especially Mo. The highest Mo content occurs in the core from the greatest water depth, an effect of anoxic conditions during deposition. The Fe/Mn ratio was shown to be ambiguous as an indicator of past redox conditions. Since biogenic silica shows large variablity in contemporaneously deposited sediments, this parameter cannot be used as a proxy for the past production of siliceous algae in the Baltic Sea.     

MODELLING RUNOFF GENERATION ON SMALL AGRICULTURAL CATCHMENTS: CAN REAL WORLD RUNOFF RESPONSES BE CAPTURED?

This paper focuses on the problem of quantifying real world catchment response using a distributed model and discusses the ability of the model to capture that response. The rainfall–runoff responses of seven small agricultural catchments in the eastern wheatbelt region of south-western Australia are examined. The variability in runoff generation and the factors that contribute to that variability (i.e. rainfall intensity, soil properties and topography) are investigated to determine if their influence can be captured in a mathematical model. The spatially distributed rainfall–runoff model used in this study is based on the TOPMODEL concepts of Beven and Kirkby (1979), and simulates runoff generation by both the infiltration excess and saturation excess mechanisms. Simulations with the model revealed the highly complex nature of catchment response to rainfall events. Runoff generation was highly heterogeneous in both space and time, with the runoff response being governed by the spatial variability of soil properties and topography, and by the temporal variation in rainfall intensity. Although the model proved capable of simulating catchment response for many events, the investigation has demonstrated that not all aspects of the variability associated with agricultural catchments (particularly the effects of land management) can be captured using this relatively simple model. © 1997 by John Wiley & Sons, Ltd     

Composition and source of the clay-sized fraction of Saalian till in The Netherlands

The clay mineral composition of a number of Saalian till samples from The Netherlands shows a large variation in smectite and illite abundances. The samples were selected according to their source area, as inferred from coarse erratic components. Specimens containing primarily rock fragments indicative of East and East-Central Baltic areas are associated with no or low smectite percentages in their clay fractions. Those with mainly flint and rock fragments indicative of the West Baltic areas have high smectite contents in the clay. In general the smectite-rich material is found in the lower portions of the Saalian till bed, whereas the smectite-poor occurs in the upper portions. The linkage between very fine and coarse till components in the upper smectite-free parts of the till suggests that this entire till has been subjected to englacial transport over distances of at least 1000 km.     

Late Quaternary glacial history of the central west coast of Jameson Land, East Greenland

The Late Quaternary ( c . 130,000–10,000 BP) glacial history of the central west coast of Jameson Land, East Greenland, is reconstructed through glacial stratigraphical studies. Seven major sedimentary units are described and defined. They represent two interglacial events (where one is the Holocene). one interstadial event and two glacial events. The older interglacial event comprises marine and fluvial sediments, and is correlated to the Langelandselv interglacial, corresponding to oxygen isotope sub-stage 5e. It is followed by an Early Weichselian major glaciation during the Aucellaelv stade, and subsequently by an Early Weichselian interstadial marine and deltaic event (the Hugin Sø interstade). Sediments relating to the Middle Weichselian have not been recognized in the area. The Hugin Sø interstade deposits have been overrun by a Late Weichselian ice advance, during the Flakkerhuk stade, when the glacier, which probably was a thin, low gradient fjord glacier in Scoresby Sund, draped older sediments and landforms with a thin till. Subsequent to the final deglaciation, some time before 10,000BP, the sea reached the marine limit around 70 m a.s.l., and early Holocene marine, fluvial and littoral sediments were deposited in the coastal areas.     

Magnetic measurements recording Late Holocene man-induced erosion in S. Greenland

Magnetic measurements have been carried out on four lake sediment successions in interior S. Greenland. The longest record extends back to 7000 C¹⁴ years BP. Based on the magnetic susceptibility six units have been distinguished. The results of the magnetic analyses show a close correlation between the four investigated sites, corroborated by pollen stratigraphy and radiocarbon dates. The period after the deglaciation, lasting for some thousand years, was characterized by sparse vegetation on the unstable soils. Erosion rates were high as revealed by the high concentration of magnetic minerals. Along with the spreading of dwarf-shrub heaths the soils stabilized, reducing the eroding capacity, and are seen as decreasing concentrations of magnetic minerals in the sediments. During the subsequent period there seems to have been an equilibrium between climate and vegetation resulting in minimal erosion. The arrival of the Norsemen in c. AD 1000, introducing cattle and cutting down the 'forests', seems to have caused a disturbance in the fragile environment. It destroyed the attained equilibrium and is seen in the sediment records as an increase in magnetic concentrations. Due to the disappearance of the Norsemen in the 15th century, or slightly later, a new phase of soil stabilization began. A new and still ongoing phase of even more severe soil erosion is connected with the reappearance of man, now as a sheep breeder, in the beginning of this century. Magnetic concentrations are again high, being in the same order of magnitude as during early Holocene time.     

Palaeomagnetic records from two varved clay sequences in the Middle Swedish ice marginal zone

Two varved clay sequences, at Rystad and Tottnäs, situated in the Middle Swedish ice marginal zone were analysed palaeomagnetically. Two parallel profiles were sampled and analysed at each site. The varved clay at Rystad was dated by floating varve chronology. The varves at Tottnäs can be linked to Swedish time scale, expressed in calendar years B.P. Due to the distance between the sites they cannot be correlated by means of varve diagrams. Palaemagnetic methods were used as an alternative. Based an AF demanetization of pilot samples, the palaeomanetic to be too low, in the order of15, compared to the site latitude. At Tottnäs the inclination records are very close to the expected inclination with respect to the site latitude. Because of a systematic inclination error in the Rystad profiles the correlation was based on the declination records. Statistical comparisons of these records between the two sites indicate that the sediment successions are partly synchronous. It is concluded that the deglaciation at Tottnäs started c. 130 years earlier than at Rystad. This mean that the Swedish ice marginal zone east of Rystad will have a more northeasterly extension than previously thought.     

The glacially sculptured landscape in Dronning Maud Land, Antarctica, formed by wet-based mountain glaciation and not by the present ice sheet

The glacial landscape beneath the Maudheimvidda ice sheet in East Antarctica was most probably formed during a more temperate phase of Antarctic glaciation than the present. Overdeepened glacial cirques and U-shaped valleys are found in the Heimefrontfjella and Vestfjella mountain ranges. These glacial landforms, located beneath the ice sheet, have been mapped with radio-echo sounders. The present ice sheet covering these landforms is cold and frozen to its bed, and has a negligible erosive effect on the substrate. Ice sheet thickening during the Quaternary glacial periods is not believed to have caused any significant increase in erosion at the investigated sites. Instead, the glacial morphology was most likely formed by smaller, temperate glaciers when the Antarctic climate was warmer than at present. Datings of foraminifera and ash layers from the Transantarc-tic Mountains indicate that the present cold ice sheet was formed 2.5 Ma years ago. Other studies imply that a cold Antarctic ice sheet has lasted even longer. The glacial landforms in Maudheimvidda may thus be of a pre-Quaternary age.