The influence of long emersion on biota, ammonium fluxes and nitrification in intertidal sediments of Marennes-Oléron Bay, France

A comparative study between waterlogged and reflooded intertidal sediments was undertaken in March and June 1999 through statistical analysis of selected sediment parameters (biota, salinity, O2, Eh), pool sizes and benthic fluxes of nutrients (NH4+, NO2-, NO3-) and nitrification rates. In March samples, absence of polychaetes and oligochaetes from upper sediment horizons were due to erosional events sweeping away surface sediments. Presence of richer annelid assemblages in June samples indicated more stable hydrodynamic conditions that favoured the development of benthic microalgae biofilms. Dewatering of sediments during a 3-day emersion period promoted a salinity rise on top layers, migration of pore water ions towards the sediment surface, and created sediment fissures that accelerated water exchange on reflooding. Reflooded and waterlogged sediment systems were comparable with respect to the release of NH4+ to overlying water but were different with respect to nitrification rates. Sediment-water NH4+ fluxes were higher (P = 0.011) in March (3.3 mmol m(-2) day(-1) compared to June (1.4 mmol m(-2) day(-1) due to higher macrofauna biomasses and lower benthic microalgae concentrations in March samples. Potential nitrification rates (range from 19 to 60 mmol NO3- (-2) day(-1)) were not statistically different between March and June. A thinner oxic layer in reflooded compared with waterlogged systems reflects a decrease of O2 diffusion into sediment at high salinities which resulted in the fall of the actual nitrification rates (P < 0.05). Our data suggest that long term dessication of intertidal sediments may depress the nitrification process at the ecosystem level.     

南极西部埃尔斯沃思山脉罗斯造山运动的地层学证据：冈瓦纳古太平洋边缘的演化

南极西部埃尔斯沃思山脉罗斯造山运动的地层学证据：冈瓦纳古太平洋边缘的演化Ｐ．Ｍ．Ｇｏｌｄｓｔｒａｎｄ等冈瓦纳重建过程中存在着一个长期的疑问，即埃尔斯沃思山脉的古生代地层的明显整合与横贯南极山脉的大地构造史相矛盾，这两座山脉在中生代冈瓦纳分裂之前通常认...     

DNA adducts in mussels and fish exposed to bulky genotoxic compounds

Biological and procedural factors can influence DNA adduct detection in aquatic organisms. Among them, functional structure and metabolic traits represent major biological determinants for adducts formed by lipophilic pro-mutagenic contaminants. In detecting DNA adducts through the 32P-postlabelling assay, efficiency in DNA purification, digestion, labelling, as well as adduct enrichment and quantification may explain differences between independent studies. Reference DNA adducts have been used to verify some 32P-postlabelling aspects. Data obtained for mussels and fish treated with benzo[a]pyrene (B[a]P) and environmentally exposed to genotoxins confirm the above assertions. Although the 32P-postlabelling assay cannot be proposed for routine biomonitoring it appears a reliable and very sensitive index of exposure to genotoxic pollutants in both fish and mollusks.     

Identification of SNARE proteins in fish-Tilapia Oreochromis niloticus

1 IntroductionSecretion is a basic function of all living organ-isms. In fish, like in mammals, neurotransmitters,hormones, enzymes and other macromolecules aresecreted in several neuronal, neuroendocrine and ex-ocrine organs and tissues located in brain,…     

末次间冰期之后劳伦和科迪勒拉冰盖的形成与发展

末次冰期年代确定中的一个基本问题是能被应用于确定事件年代而又大于放射性碳的测定限度的可靠的测年方法太少，最近10-20a来新测试技术的发展和应用已加深了我们对晚第四纪事件的了解，然而，对于确定事件的年代和分析这些事件依然受到限制．     

Evidence for enhanced bioavailability of trace elements in the marine ecosystem of Deception Island, a volcano in Antarctica

This study assessed whether trace elements present at Deception Island, an active submarine volcano in the Antarctic Peninsula, show enhanced biological availability to the local marine community. Using a weak acid extraction method to dissolve organic material and leach associated but not constitutive trace elements of sediments, fifteen elements were measured from seafloor sediment, seawater particulates, and tissues of benthic (bivalves, brittlestars, sea urchins) and pelagic (demersal and pelagic fishes, krill) organisms collected in the flooded caldera. The highest element concentrations were associated with seafloor sediment, the lowest with seawater particulates and organism tissues. In the case of Ag and Se, concentrations were highest in organism tissue, indicating contamination through the food chain and biomagnification of those elements. The elements Al, Fe, Mn, Sr, Ti, and to a lesser extent Zn, were the most concentrated of the trace elements for all sample types. This indicates that the whole ecosystem of Deception Island is contaminated with trace elements from local geothermal activity, which is also reflected in the pattern of element contamination in organisms. Accordingly, element concentrations were higher in organisms collected at Deception Island compared to those from the neighboring non-active volcanic King George Island, suggesting that volcanic activity enhances bioavailability of trace elements to marine organisms. Trace element concentrations were highest in digestive tissue of organisms, suggesting that elements at Deception Island are incorporated into the marine food web mainly through a dietary route.     

南海红树林内生真菌1356＃代谢产物的研究

从南海红树林内生真菌1356＃分离出两种鞘胺醇（A，B）和三种环二肽（C，D，E）。它们的结构通过IR，MS，1D NMR和2D NMR谱推导出来，（3'E,4E)-1-(-D-吡喃葡萄糖基－3－羟基－2－（2'-羟基十八碳酰基）氨基－10－甲－3',4,9-十八碳三烯（A）是一种新的鞘胺醇甙。     

Induction of phenol-type sulfotransferase and glucuronosyltransferase in channel catfish and mummichog

Conjugation of phenolic xenobiotics and metabolites through sulfation and glucuronidation is an important biotransformation pathway. Sulfotransferases (SULT) are generally considered non-inducible, while some UDP-glucuronosyltransferase (UGT) isoenzymes are co-induced with cytochrome P450-1A by Ah-receptor ligands. To test these assumptions for two fish species, we measured sulfation and glucuronidation of 9-hydroxybenzo[a]pyrene in 3-methylcholanthrene (3-MC) treated channel catfish (Ictalurus punctatus) and in mummichog (Fundulus heteroclitus) from the creosote contaminated Atlantic Wood site in the Elizabeth River, VA. The results show a significant induction of both UGT and SULT activity in 3-MC treated catfish, linked to the expected induction of EROD activity. In mummichog, significant induction of UGT was measured at the contaminated site over the reference site (King's Creek, VA), as well as extremely low SULT activities at both sites. Western blots, using a polyclonal antibody for catfish phenol-type SULT, confirmed the absence of phenol-type SULT in mummichog. Residual, though slightly inducible, SULT activity may be attributed to other SULT isoforms.     

重量稀释的和浓缩的标准海水的电导率同盐度和温度的函数关系

在-2℃至35℃的整个海洋温度范围和0至42‰S盐度范围内测量准确已知盐度的海水样品电导率和同温度下标准海水电导率的比值R_(s.t.o)。盐度S<35‰的海水样品是由蒸馏水准确重量稀释标准海水制备的,快速蒸发标准海水制备高盐度海水样品继而重量稀释到已经确定的<35‰S范围。推导出了非常准确地表示1～42‰S和全部温度范围内的S与R_(s.t.o)关系式,即 S=f_1(R_(s.t.o)) f_2(R_(s.o,t.)t)=sum from n=0 to 5 a_1R~(a/2) △t/(1 k△t)sum from n=0 to 5 b_nR~(n/2)式中△t=t-15℃,R=R_(s.t.o),只有第一项f_1要求15℃。也确定了温度对标准海水电导率的影响,用t的四次方程非常准确地表示温度t时的电导率的比值的r_(tt)(C_(35.t.o)/C_(35.15.o)),即:(?)_t=sum from n=0 to 4 C_nt~n 这两个方程足以满足常压下所有盐度测量。     

Minor constituents in the Martian atmosphere from the ISM/Phobos experiment

Global Martian atmospheric results derived from the infrared imaging spectrometer ISM flown aboard the Phobos 2 Soviet spacecraft are presented. Over low altitude regions the expected CO mixing ratio of (8 +/- 3) x 10(-4) is measured. Variations of the 2.35-micrometers feature are inconsistent with this value over the Great Martian Volcanoes. If the 2.35-micrometers band is entirely attributable to carbon monoxide, the CO mixing ratio is typically depleted by a factor of 3 over these high altitude areas. Orography should play a major role in the existence of this CO "hole." If, however, these spectral variations at 2.35 micrometers are due to the surface composition, the fraction of the surface covered by the responsible mineral must smoothly decrease as the surface elevation decreases. This phenomenon implies a strong interaction between the surface and the atmosphere for the Great Martian Volcanoes. Diurnal behavior and latitudinal variations of water vapor are globally consistent with Viking measurements. During the Phobos observations, the water vapor amounts over the bright equatorial regions range around 11 pr-micrometers during the day. These amounts are slightly larger than those inferred from 1976 to 1979. The lack of global dust storms during 1988-1989 could explain the enhancement of H2O in the atmosphere.