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131.
Induction of vitellogenin in vivo and in vitro in the model teleost medaka (Oryzias latipes): comparison of gene expression and protein levels 总被引:5,自引:0,他引:5
Quantification of the egg yolk precursor vitellogenin (VTG) in fish has become a standard technique to detect estrogenic effects of known chemicals and environmental samples. In the present study, we have analysed VTG induction by estradiol, ethynylestradiol and genistein exposure in the model teleost medaka (Oryzias latipes) and demonstrate that the medaka is a suitable model system to analyse estrogenic effects. By comparing VTG gene expression and protein levels we show that in principal both techniques can be used to study VTG induction in vivo (juvenile and adult males) and in vitro (primary cultures of male liver cells). If a short term in vivo or in vitro exposure is performed, detection of mRNA might be sufficient. For long term studies with the need to detect weak estrogenic chemicals and a precise quantification, immuno-chemical detection may be favoured. 相似文献
132.
Jeffrey M. Napp Christine T. Baier Richard D. Brodeur Kenneth O. Coyle Naonobu Shiga Kathy Mier 《Deep Sea Research Part II: Topical Studies in Oceanography》2002,49(26)
The southeastern Bering Sea shelf ecosystem is an important fishing ground for fin- and shellfish, and is the summer foraging grounds for many planktivorous seabirds and marine mammals. In 1997 and 1998, Northern Hemisphere climate anomalies affected the physical and biological environment of the southeastern Bering Sea shelf. The resulting anomalous conditions provided a valuable opportunity to examine how longer-term climate change might affect this productive ecosystem. We compared historical and recent zooplankton biomass and species composition data for the southeastern Bering Sea shelf to examine whether or not there was a response to the atmosphere–ocean–ice anomalies of 1997 and 1998. Summer zooplankton biomass (1954–1994) over the southeastern shelf did not exhibit a decline as previously reported for oceanic stations. In addition, zooplankton biomass in 1997 and 1998 was not appreciably different from other years in the time series. Spring concentrations of numerically abundant copepods (Acartia spp., Calanus marshallae, and Pseudocalanus spp.), however, were significantly higher during 1994–1998 than 1980–1981; spring concentrations of Metridia pacifica and Neocalanus spp. were not consistently different between the two time periods. Neocalanus spp. was the only taxon to have consistent differences in stage composition between the two time periods—CV copepodites were much more prevalent in May of the 1990s than early 1980s. Since relatively high zooplankton concentrations were observed prior to 1997, we do not attribute the high concentrations observed in the summers of 1997 and 1998 directly to the acute climate anomalies. With the present data it is not possible to distinguish between increased production (control from below) and decreased predation (control from above) to explain the recent increase in concentrations of the species examined. 相似文献
133.
An analysis of published and original data on the meiobenthos abundance in the depth interval from 100 to 9807 m (in total, 665 records, 445 of them obtained for depths exceeding 1000 m) revealed general regularities in its distribution. The influence of the sampling and data processing methods on the quantitative estimates of the meiobenthos abundance is considered to demonstrate changes in the proportions of the main meiobenthic taxa at different depths and to characterize latitudinal changes in the meiobenthos abundance. The dependence of the abundance of free-living nematodes, the most abundant group of metazoan meiobenthos, on trophic conditions is analyzed. No significant differences in the meiobenthos abundance in the samples obtained by box-and multicorers are established. It is shown that the share of nematodes in metazoan meiobenthos communities increases with the depth. In temperate latitudes, a distinct maximum in the population density confined to depths exceeding 1 km is observed. The quantitative distribution of the meiobenthos at the depths gradient is controlled by the bottom macrotopography and trophic conditions. 相似文献
134.
Michael S. Dinniman John M. Klinck Walker O. Smith Jr. 《Deep Sea Research Part II: Topical Studies in Oceanography》2003,50(22-26):3103
Transport of warm, nutrient-rich Circumpolar Deep Water (CDW) onto Antarctic continental shelves and coastal seas has important effects on physical and biological processes. The present study investigates the locations of this transport and its dynamics in the Ross Sea with a high-resolution three-dimensional numerical model. The model circulation is forced by daily wind stress along with heat and salt fluxes calculated from atmospheric climatologies by bulk formulae. All surface fluxes are modified by an imposed climatological ice cover. Waters under the Ross Ice Shelf are not included explicitly, but their effect on temperature and salinity is imposed in a buffer zone at the southern end of the model domain. A simple nutrient uptake is calculated based on the climatological chlorophyll distribution and Monod uptake kinetics.Model circulation is strongly affected by bottom topography, due to weak stratification, and agrees with schematics of the general flow and long-term current measurements except near the southern boundary. The sea-surface temperature is similar to satellite estimates except that the warmest simulated temperatures are slightly higher than observations. There is a significant correlation between the curvature of the shelf break and the transport across the shelf break. A momentum term balance shows that momentum advection helps to force flow across the shelf break in specific locations due to the curvature of the bathymetry (that is, the isobaths curve in front of the flow). For the model to create a strong intrusion of CDW onto the shelf, it appears two mechanisms are necessary. First, CDW is driven onto the shelf at least partially due to momentum advection and the curvature of the shelf break; then, the general circulation on the shelf takes the CDW into the interior. 相似文献
135.
V. A. Dulov S. I. Klyushnikov V. N. Kudryavtsev O. V. Shulgin O. G. Shcherbak 《Physical Oceanography》1991,2(4):269-282
An overview of experiments is given on the observation of the dependence of the intensity of wind wave breaking on large-scale
currents' inhomogeneities and atmospheric boundary layer stratification. The data were interpreted using a model in which
the variance of the wind wave breaking intensity reflects fluctuations in the influx of energy to wind waves due to various
factors.
Translated by V. Puchkin. 相似文献
136.
The proposed numerical model simulates the short-term temporal changes in shoreline position due to a structure interrupting the longshore sediment flux. The impacts of both the groin-type construction and underwater trench of arbitrary orientation relative to the shore are discussed. In order to estimate the sediment mass trapped by the structure, a submodel of the longshore sediment transport induced by a random wave field is developed. The contribution of the surface roller in momentum balance as well as in sediment suspension is included. The shoreline changes are computed from the equation deduced from the mass conservation. The perturbations in the longshore sediment discharge caused by a structure are assumed to concentrate within some boundary area of which the spatial scale is proportional to the structure's length until the latter is exceeded by the width of the sediment flux. It is shown in particular that the total effect of a long trench (channel) and a pier in its nearshore part results in general shoreline recession except for the vicinity of a pier. The model is tested against the laboratory data of Baidei et al. (1994) and applied to the Baidara Bay coast (Kara Sea) where a pipeline would be designed. 相似文献
137.
We present the results of experimental investigations of the characteristics of turbulence in the layer of wave-induced mixing.
The data on the fluctuations of velocity, temperature, and conductivity are obtained with the help of a Sigma-1 measuring complex. The computed values of the dissipation rate of turbulent energy are compared with different models proposed
for the subsurface layer. It is shown that the available models fail to guarantee satisfactory agreement of the numerical
results with the experimental data for the layer of active wave action and, in particular, in the presence of swell. This
leads us to the conclusion concerning the necessity of parametrization and assimilation of more complete data on the state
of the sea surface, the structure of currents, and the surface layer of the atmosphere in the models.
__________
Translated from Morskoi Gidrofizicheskii Zhurnal, No. 2, pp. 15–28, March–April, 2007. 相似文献
138.
Mucous macroaggregates, recently observed in the northern Adriatic in summer of 1997 and late spring of 2000, are primarily the product of phytoplankton (diatom) exudates during favourable environmental conditions in late spring, and can be viewed as macrogels. The FT-IR and 1H-NMR spectra of macroaggregate samples from the northern Adriatic, collected in different formation stages in July 1997, August 1997, September 1997 and June 2000, as well as of cultured diatom Skeletonema costatum, showed that they are similar and composed of aliphatic components and polysaccharides bonded through carboxylic and amide groups, and organosilicon compounds. The stability of macrogels of macroaggregates is, according to FT-IR and X-ray analyses, most probably enhanced by interactions with entrapped particles of calcite, quartz and clay minerals. These organic-mineral associations seem to be important for mucous phenomena. According to 1H-NMR spectra, the temporal differences in macroaggregate composition show a relative increasing percentage of organosilicon compounds and aliphatic components bonded to carbohydrates through ester and amide groups, and an increasing ratio between aliphatic structures and carbohydrates. This indicates that aliphatic chains bonded to Si and carbohydrates may contribute to the persistence and stability of macroaggregates in the summer stratified waters in the northern Adriatic, while the temporal decrease of carbohydrate content is most probably due to microbial and photochemical degradation of algal reserve polysaccharides. 相似文献
139.
The transport pathways of fine sediments (fraction <2 μm) along the Brazilian continental shelf from Ceará to the Amazon River
mouth were studied by means of clay mineral analyses. On the continental shelf southeast of the Amazon mouth, fluctuations
in clay mineral compositions reflect simple mixing between the suspended load of the North Brazil Current and sediment from
several smaller rivers. Previously, clay mineral variations west of the Amazon mouth have been explained by variable settling
velocities of different grain-size classes within the <2 μm fraction or by selective coagulation of individual clay mineral
groups. By contrast, our experiments with river bank samples show that selective coagulation does not occur in Amazon River
sediments. A more appropriate explanation for observed variations in clay mineral composition off the Amazon mouth seems to
be, similarly to that for the shelf between Ceará and the Amazon mouth, a mixing of Amazon sediments with suspended material
of the North Brazil Current. This interpretation is supported by data on clay mineral composition east and south of the Amazon
mouth, showing more affinity to sediments of the North Brazil Current than to the suspended load of the Amazon River. Additionally,
relatively low sedimentation rates and low concentrations of fine-grained sediments on the shelf suggest that high riverine
input by the Amazon River does not overprint the sediments of the North Brazil Current in this region. The strong North Brazil
Current shunts the Amazon suspended load in a north-westerly direction along the north-eastern coast of South America. Hence,
stronger sedimentation of Amazon sediments would occur only west of the river mouth. 相似文献
140.
Th sorption and export models in the water column: A review 总被引:2,自引:3,他引:2
Nicolas Savoye Claudia Benitez-Nelson Adrian B. Burd J. Kirk Cochran Matthew Charette Ken O. Buesseler George A. Jackson Matthieu Roy-Barman Sabine Schmidt Marc Elskens 《Marine Chemistry》2006,100(3-4):234
Over the past few decades, the radioisotope pair of 238U / 234Th has been widely and increasingly used to describe particle dynamics and particle export fluxes in a variety of aquatic systems. The present paper is one of five review articles dedicated to 234Th. It is focused on the models associated with 234Th whereas the companion papers (same issue) are focused on present and future methodologies and techniques (Rutgers van der Loeff et al.), C / 234Th ratios (Buesseler et al.), 234Th speciation (Santschi et al.) and present and future applications of 234Th [Waples, J.T., Benitez-Nelson, C.R., Savoye, N., Rutgers van der Loeff, M., Baskaran, M., Gustafsson, Ö., this issue. An Introduction to the application and future use of 234Th in aquatic systems. Marine Chemistry, FATE special issue]. In this paper, we review current 234Th scavenging models and discuss the relative importance of the non-steady state and physical terms associated with the most commonly used model to estimate 234Th flux. Based on this discussion we recommend that for future work the use of models should be accompanied by a discussion of the effect that model and data uncertainty have on the model results. We also suggest that future field work incorporate repeat occupations of sample sites on time scales of 1–4 weeks in order to evaluate steady state versus non-steady state estimates of 234Th export, especially during high flux events (> ca. 800 dpm m− 2 d− 1). Finally, knowledge of the physical oceanography of the study area is essential, particularly in ocean margins and in areas of established upwelling (e.g., Equatorial Pacific). These suggestions will greatly enhance the application of 234Th as a tracer of particle dynamics and flux in more complicated regimes. 相似文献