Microbial responses using denaturing gradient gel electrophoresis to oil and chemical dispersant in enclosed ecosystems

Microbial responses to the addition of oil with or without a chemical dispersant were examined in mesocosm and microcosm experiments by using denaturing gradient gel electrophoresis of bacterial ribosomal DNA and direct cell counting. When a water-soluble fraction of oil was added to seawater, increases in cell density were observed in the first 24h, followed by a decrease in abundance and a change in bacterial species composition. After addition of an oil-dispersant mixture, increases in cell density and changes in community structure coincided, and the amount of bacteria remained high. These phenomena also occurred in response to addition of only dispersant. Our results suggest that the chemical dispersant may be used as a nutrient source by some bacterial groups and may directly or indirectly prevent the growth of other bacterial groups.     

Hysteresis and Surface Shear Stresses During Snow-Particle Aeolian Transportation

Boundary-Layer Meteorology - Surface shear stresses produced by wind and particle collision play a key role in aerodynamic entrainment and splash processes. The fluid shear stress at the surface...     

Snow ripples and their contribution to the mass transport in drifting snow

Morphological characteristics of snow ripples formed by drifting snow were investigated as functions of wind velocity in a cold wind tunnel at -15 °C. Wave-length, wave height and migration rate of snow ripples increased from 5 to 20 cm, 3 to 5 mm and 1 to 8 cm/min, respectively, with increasing wind velocity from 5 to 7 m/s. Measured size distributions of snow particles in snow ripples showed sorting of large particles in ridges, suggesting that the snow ripple migration is caused by creeping of large particles. The snow drift rate caused by creep, that is, by the ripple migration, was estimated to amount, at least, to 6% of the total snow drift rate.     

Near-infrared observations of the dust coma of Comet Kohoutek (1973f) with a tilting-filter Fabry-Perot photometer

By means of narrow-band Fabry-Perot filters, which exclude the interference from molecular line fluorescence, the brightness of Comet Kohoutek (1973f) has been measured at 8560 and 8748 Å. Data reduction on the basis of averaged Mie-scattering cross sections indicates that the dust production rate was different before and after perihelion at the same heliocentric distances. This asymmetry suggests that vaporization and dust entrainment were governed by fractionation of a multicomponent mixture of parent molecules in a comparatively porous cometary nucleus.     

Interannual fluctuations in recruitment of walleye pollock in the Oyashio region related to environmental changes

The Japanese Pacific walleye pollock (Theragra chalcogramma) stock is the largest stock of this species in Japanese waters. It is a key component of the Oyashio ecosystem. In southern Hokkaido waters, these fish spawn mainly during January and February near the mouth of Funka Bay (FB), and most eggs and larvae are transported into FB. During midsummer juvenile pollock migrate along the southern coast of Hokkaido to a nursery ground on the continental shelf off eastern Hokkaido (Doto area). However, some eggs and larvae are transported southward to the Tohoku region (TR). Transport depends largely on the Oyashio, which generally flows southward along the eastern coasts of Hokkaido and Tohoku. Thus, this stock has two different recruitment routes: FB–Doto and FB–TR. In the 1980s, when the southward flow of the Oyashio was strong, the number of age-2 pollock estimated from a virtual population analysis (VPA) indicated that recruitment to the entire stock remained at a medium level. In the 1990s, when the Oyashio weakened, strong year-classes occurred in 1991, 1994, and 1995, but not in the latter half of the 1990s. Juvenile catches in the TR by commercial fisheries, which can be taken as indices of recruitment level via FB–TR, were high during the 1980s and decreased in the 1990s. Although there was no significant difference in the average number of recruits between the 1980s and the 1990s as estimated from a VPA, the recruitment patterns differed between the two decades. Here, we propose that recruitment routes of this stock shifted in response to environmental changes.     

Field observations of meteotsunami locally called “abiki” in Urauchi Bay, Kami-Koshiki Island, Japan

A seasonal scale field observation extending over a period of 82?days was conducted in Urauchi Bay on Kami-Koshiki Island, to record meteotsunami events, disastrous secondary oscillations locally known as ??abiki.?? The bay has an elongated T-shape topography with a narrow mouth opening westward to the East China Sea. The area has suffered the effects of meteotsunami causing flooding in residential area and damage to fishing fleets and facilities. A comprehensive observation system for sea level, ocean currents and barometric pressure was deployed to cover the regions within and offshore from Urauchi Bay and the open sea near the island of Mejima in the East China Sea. Vigorous meteotsunami events, where the total height exceeded 150?cm, were observed over five-day periods during the observation period. One or two hours prior to the arrival of meteotsunami events at Kami-Koshiki Island, abrupt 1?C2?hPa pressure changes were observed at the Mejima observation site. Pressure disturbances were found to travel eastward or northeastward. The propagation speed was found to nearly coincide with that of ocean long waves over the East China Sea, and as a result, resonant coupling should be anticipated. The incoming long waves were also amplified by geometric resonance with eigen oscillations inherent in the T-shape topography of Urauchi Bay.     

Sea-floor methane blow-out and global firestorm at the K–T boundary

A previously unsuspected source of fuel for the global firestorm recorded by soot in the Cretaceous–Tertiary impact layer may have resided in methane gas associated with gas hydrate in the end-Cretaceous seafloor. End-Cretaceous impact-generated shock and megawaves would have had the potential to initiate worldwide oceanic methane gas blow-outs from these deposits. The methane would likely have ignited and incompletely combusted. This large burst of methane would have been followed by longer-term methane release as a part of a positive thermal feedback in the disturbed ocean-atmosphere system.     

Study of the effect of water-soluble fractions of heavy-oil on coastal marine organisms using enclosed ecosystems,mesocosms

Mesocosm facilities composed of 4 experimental and 2 reservoir tanks (1.5 m in diameter, 3.0 m in depth and 5 tons in capacity) made of FRP plastics, were constructed in the concrete fish rearing pond in the Fisheries Laboratory, The University of Tokyo. The water-soluble fraction of Rank A heavy residual oil was formed by mixing 500 g of the oil with 10 l of seawater, which was introduced to the 5000 l-capacity tanks. Experimental Run 4 was conducted from May 31 to June 7, 2000. Oil concentrations in the tanks were 4.5 microg/l called LOW, and 13.5 microg/l, called HIGH tank. Bacterial growth rates very quickly accelerated in the HIGH tank just after the loading of oil which corresponded with a high increase of bacterial cells in the same tank after 2 days. Later, bacterial numbers in HIGH tank rapidly decreased, corresponding with the rapid increase of heterotrophic nano-flagellates and virus numbers on the same day. Sediment traps were deployed at the bottom of the experimental tanks, and were periodically retrieved. These samples were observed both under light microscope and epi-fluorescent microscope with UV-excitation. It was observed that the main components of the vertical flux were amorphous suspended matter, mostly originating from dead phytoplankton and living diatoms. It was further observed from the pictures that vertical transport of oil emulsions were probably conducted after adsorption to amorphous suspended matter and living diatoms, and were settling in the sediment traps at the bottom of the tanks. This means that the main force which drives the soluble fraction of oil into bottom sediment would be vertical flux of such amorphous suspended particles and phytoplankton. Further incubation of the samples revealed that the oil emulsions were degraded by the activity of autochtonous bacteria in the sediment in aerobic condition.     

Tsunami deposits from the 1993 Southwest Hokkaido earthquake and the 1640 Hokkaido Komagatake eruption,northern Japan

The southwest Hokkaido tsunami of July 12th, 1993, left continuous onshore sand deposits along the west coast of Oshima Peninsuka, Hokkaido, northern Japan. We investigated spatial distribution and lithofacies of the new tsunami deposits for its identification of ancient tsunami deposits. An eyewitness acount and bent plants helped our interpretation of the onshore tsunami behavior. We regard the following properties as typical of the coastal tsunami sand deposits: (1) The deposits cover the surface almost continuously on gentle topography. (2) Deposit thicknesses and mean grain sizes descrease with distance from the sea. (3) Deposit thicknesses and lithofacies vary greatly across local surface undulation. (4) Graded bedding reflecting tsunami runup and backwash is present in thick deposits. (5) The deposits are widely distributed along the coast and extend inland several tens of meters to 100 m. We examined a candidate for the paleo-tsunami deposits associated with the 1640 Komagatake eruption, and confirmed that the similar patterns are typical of ancient tsunami deposits.     

New age constraints on the cooling and unroofing history of the Trans-Himalayan Ladakh Batholith (Kargil area), N. W. India

Thermotectonic history of the Trans-Himalayan Ladakh Batholith in the Kargil area, N. W. India, is inferred from new age data obtained here in conjunction with previously published ages. Fission-track (FT) ages on apatite fall around 20±2 Ma recording cooling through temperatures of ∼100°C and indicating an unroofing of 4 km of the Ladakh Range since the Early Miocene. Coexisting apatite and zircon FT ages from two samples in Kargil show the rocks to have cooled at an average rate of 5–6°C/Ma in the past 40 Ma. Zircon FT ages together with mica K−Ar cooling ages from the Ladakh Batholith cluster around 40–50 Ma, probably indicating an Eocene phase of uplift and erosion that affected the bulk of the batholith after the continental collision of India with the Ladakh arc at 55 Ma. Components of the granitoids in Upper Eocene-Lower Oligocene sediments of the Indus Molasse in Ladakh supports this idea. Three hornblende K−Ar ages of 90 Ma, 55 Ma, and 35 Ma are also reported; these distinctly different ages probably reflect cooling through 500–550°C of three phases of I-type plutonism in Ladakh also evidenced by other available radiometric data: 102 Ma (mid-Cretaceous), 60 Ma (Palaeocene), and 40 Ma (Late Eocene); the last phase being localised sheet injections. The geodynamic implications of the age data for the India-Asia collision are discussed.