2012年夏季楚科奇海浮游动物垂直迁移及上升流对其生物量的影响

The diel vertical migration(DVM) of zooplankton and the influence of upwelling on zooplankton biomass were examined using water column data of current velocity and mean volume backscattering strength(MVBS)collected by moored acoustic Doppler current profilers(ADCPs) deployed in the southeastern Chukchi Sea during the 5th Chinese National Arctic Research Expedition(CHINARE) in summer 2012, combined with the satellite observational data such as sea surface temperature(SST), wind, and chlorophyll a(Chl a). Hourly acoustic data were continuously collected for 49-d in the mooring site. Spectral analysis indicated that there were different migrating patterns of zooplankton, even though precisely classifying the zooplankton taxa was not available. The prevailing 24-h cycle corresponded to the normal DVM with zooplankton swimming upwards at sunrise and returning to deep waters at sunset. There was a clear DVM in the upper 17 m of the water column during the period with distinct day-night cycles, and no active DVM throughout the water column when the sun above the horizon(polar day), suggesting that light intensity was the trigger for DVM. Also there was a second migrating pattern with 12-h cycle. The upwelling event occurring in the northwest of Alaskan coastal area had important influence on zooplankton biomass at the mooring site. During the upwelling, the SST close to the mooring site dropped significantly from maximal 6.35°C to minimal 1.31°C within five days. Simultaneously, there was a rapid increase in the MVBS and Chl a level, suggesting the aggregation of zooplankton related to upwelling.     

A cDNA microarray technique applied for analysis of global gene expression profiles in tributyltin-exposed ascidians

To analyze global gene expressions, we constructed a cDNA microarray from a basal chordate, the ascidian Ciona intestinalis. Ciona is a cosmopolitan species and a genomic analysis of Ciona revealed that ascidians had approximately 15,500 protein-coding genes. Our "Ciona intestinalis cDNA chip version 1 (Ci cDNA chip ver. 1)" has arrayed 13,400 unique Ciona cDNAs. To establish a detection system for gene expression profiles in wild ascidians using a cDNA microarray, we analyzed gene expressions in the whole body of Ciona adults after exposure to 100 nM tributyltin (TBT) for 24 h. In our preliminary array data using Ci cDNA chip ver. 1, we found more than 200 genes that showed strong differential expressions. These genes encoded proteins that were concerned with stress response, detoxification, oxidoreduction reaction, biosynthesis, and catabolism. This, the first large cDNA microarray of this animal, should facilitate analyses of global gene expressions following exposure to TBT.     

Morphological and molecular evidence supports the first occurrence of two fishes,Siganus sutor(Valenciennes, 1835)and Seriolina nigrofasciata(Rüppell, 1829)(Actinopterygii:Perciformes), from marine waters of Odisha coast,Bay of Bengal,India

Marine ecosystems provide a wide variety of diverse habitats that frequently promote migration and ecological adaptation. The extent to which the geographic distribution of marine organism has reshaped by human activities remains underappreciated. The limitations intrinsic to morphology-based identification systems have engendered an urgent need for reliable genetic methods that enable the unequivocal recognition of fish species,particularly those that are prone to overexploitation and/or market substitution. In the present study, however, an attempt has been taken to identify two locally adapted fish species, Siganus sutor(Valenciennes, 1835) and Seriolina nigrofasciata(Rüppell, 1829) of order Perciformes, which happens to be the first record in Odisha coast,Bay of Bengal. The diagnostic characteristics of Siganus sutor are: dorsal fin XIII-10, anal fin VII-9, pectoral fin 15,pelvic fin II-3, while that of Seriolina nigrofasciata dorsal fin VI-I-35, anal fin I-17, pectoral fin 16, pelvic fin 5. All COI barcodes generated in this study were matched with reference sequences of expected species, according to morphological identification. Bayesian and likelihood phylogenetic trees were drawn based on DNA barcodes and all the specimens clustered in agreement with their taxonomic classification at the species level. The phylogeographic studies based on haplotype network and migration rates suggest that both the species were not panmitic and the high-frequency population distribution indicates successful migration. The result of this study provides an important validation of the use of DNA barcode sequences for monitoring species diversity and changes within a complex marine ecosystem.     

Cytochrome P450 differences in normal and imposex-affected female whelk Buccinum undatum from the open North Sea

Normal and imposex-affected female Buccinum undatum were sampled from the open North Sea at three locations, one with low, and two with high shipping densities. Cytochrome P450 components and P450 aromatase activity were determined in the microsomal fractions isolated from pooled digestive gland/gonads. Cytochrome P450 aromatase activity was significantly higher (P < 0.05) in normal females collected in the low shipping density area (1,325 +/- 295 fmol/h/mg protein) than levels from imposex animals from a high shipping density area (620 +/- 287 fmol/h/mg protein). A negative correlation was found between aromatase activity and organotin body burden (r = -0.99). Levels of CYP450, cytochrome b5 and NADPH cytochrome c reductase activity did not show differences among groups. This is the first field evidence of depressed aromatase activity in imposex affected females, although additional research under laboratory controlled conditions is required to fully understand the mechanisms underlying the development of imposex in this species.     

Evaluation of sampling devices for the determination of polycyclic aromatic hydrocarbons in surface microlayer coastal waters

The sea surface microlayer (SML) may play an important role on the transport and fate of persistent organic pollutants in the marine environment. In order to evaluate the appropriateness of a number of sampling devices for the analysis of 14 parent polycyclic aromatic hydrocarbons (3-5 aromatic rings), marine SML waters were sampled using a glass plate, a rotating drum/roller, a metal screen and a surface slick sampler. The underlying waters were also sampled for the determination of the corresponding enrichment factors (EF = [C](microlayer)/[C](underlying water)). The EFs were phase dependent, ranging from 1 to 3 for the dissolved phase and between 4 and 7 for the particulate phase. In order to better assess the performance of the different sampling methods, in terms of phase partitioning, the truly dissolved and colloidal phases were also estimated. Generally, no significant differences were found for the enrichment factors provided by the different methods, due to the observed large variability in concentrations that can be attributed to small-scale coastal processes. However, the metal screen is recommended as the most efficient sampling method for the study of PAHs taking into account the amount of water collected versus time.     

通过对重力波曳力的参数化来减小数值天气预报和大气环流模式中的系统性误差

一、引 言 由于地形性激发的中尺度重力波的中断,在加拿大气候中心大气环流谱模式中有时候采用了波的曳力效应的参数化。这一参数化的细节以及它在大气环流模式气候模拟中影响的讨论,可见Boer等人(1985)的工作。 最近,人们利用激发重力波次尺度地形的更好的描绘,来修改这一参数化方案。还有,使用Lindzen(1981)的波饱和概念来描述曳力效应。这一关于地形重力波曳力参数化的作法已在加拿大气候中心的大气环流谱模式中广泛试验,在加拿大气象中心的业务的谱数值预报模式中也正在普遍进行试验。本文的目的所在于概述在这些模式中正在普遍使用的波的曳力方案,并且证明这一方案在消除中期预报和大气环流模拟的系统性误差方面的用处。     

Coral reef recovery status in south Andaman Islands after the bleaching event 2010

The Andaman and Nicobar Islands are one of the Union Territories of India, located in the eastern part of the Bay of Bengal. In 2010 summer, the increment in sea surface water temperature (up to 34℃) resulted in the bleaching of about 74% to 77% of corals in the South Andaman. During this event, coral species such as Acropora cerealis, A. humilis, Montipora sp., Favia pallida, Diploastrea sp., Goniopora sp. Fungia concinna, Gardineroseries sp., Porites sp., Favites abdita and Lobophyllia robusta were severely affected. This study is to assess the recovery status of the reef ecosystem by estimating the percentage of Live Coral cover, Bleached coral cover, Dead coral with algae, Rubble, Sandy flat, Algal assemblage and other associated organisms. The sedimentation rate (mg cm-2 d-1) and coral coverage (%) were assessed during this study period. The average sedimentation rate was ranged between 0.27 and 0.89 mg cm-2 d-1 . The observed post bleaching recovery of coral cover was 21.1% at Port Blair Bay and 13.29% at Havelock Island. The mortality rate of coral cover due to this bleaching was estimated as 2.05% at Port Blair Bay and 9.82% at Havelock Island. Once the sea water temperature resumed back to the normal condition, most of the corals were found recovered.     

Mushroom production as an alternative for rural development in a forested mountainous area

Wild mushrooms are recognized as important non-wood forest products in mountainous ecosystems, but their real potential for generating rural economies has not been fully evaluated due to the difficulties in obtaining reliable productivity data, minimizing their true potential as contributor to rural economies. Mushroom yield models based on large data series from Pinus forest ecosystems in the region of Catalonia(Spain), combined with data from the Spanish National Forest Inventory allow us to estimate the potential mushroom productivity by forest ecosystems. The results of 24,500 tons/yr of mushrooms of which 16,300 tons are classified as edible and 7,900 tons are commonly marketed demonstrate the importance of mushroom productions in Catalonian pine forests, mostly located in mountainous areas where the development of agricultural activities is limited. Economic mushroom value is estimated at 48 million € for the edible mushroom and 32 million € for those corresponding to marketable yields, confirming the potential of this non-wood forest product. These production results and corresponding economic values provide a basis for the incorporation of wild mushrooms as significant non-wood forest products in the development of forest policies in mountainous areas.