藻类分子生物技术两年评─—与藻类活性物质研究有关的生物技术

根据１９９２年６月－１９９４年６月国内外发表资料，评述海洋藻类活性物质研究的生物技术及最新成果。认为，海洋生物活性物质研究的重心正转向与海洋生物技术有关的研究。运用生物技术筛选、提取并生产藻类活性物质已经成为这一领域的主流。综合文献表明，藻类活性物质的生物技术正走向成熟，大量的活性物质被发现，光生物反应器将成为主要的下游生产技术，通过藻类基因工程生产药物已初现端倪。     

Parallel experiments with Thalassiosira rotula in outdoor plastic tanks: Development of dissolved free amino acids during an algae bloom

Three outdoor plastic tanks were filled with North Sea water. The water was filtered free of algae and inoculated with the diatom Thalassiosira rotula. Under nearly natural nutrient supply an exponential plankton growth occurred with realistic cell concentrations. The flexible tanks (3–4 m³, 4–5 m deep) were exposed in the German Bight at a station in the outer harbour of Helgoland. The concentration changes of dissolved free amino acids (DFAA) were determined. The direct determination method applied permitted an analysis in picomole range without loss and with good reproducibility. The preliminary concentration of DFAA amounted to 0.2 μmol dm⁻³ in all three cultures, increasing during the development of T. rotula to 0.9–1.3 μmol dm⁻³ and decreasing in the stationary phase back again to smaller values. A high degree of temporal variation in DFAA was observed in all three cultures. In spite of these rapid changes in concentration, a good parallel development could be shown for all cultures, which extended as far as the spectra of the amino acids. Relationships between DFAA and other parameters of the culture-systems were examined.     

Marine pipeline dynamic response to waves from directional wave spectra

A methodology has been developed to calculate the dynamic probabilistic movement and resulting stresses for marine pipelines subjected to storm waves. A directional wave spectrum is used with a Fourier series expansion to simulate short-crested waves and calculate their loads on the pipeline. The pipeline displacements resulting from these loads are solutions to the time-dependent beam-column equation which also includes the soil resistance as external loading. The statistics of the displacements for individual waves are combined with the wave statistics for a given period of time, e.g. pipeline lifetime, to generate probabilistic estimates for net pipeline movement. On the basis of displacements for specified probability levels the pipeline configuration is obtained from which pipeline stresses can be estimated using structural considerations, e.g. pipeline stiffness, end restraints, etc.     

Outer wave number spectrum and statistical distribution of apparent wave number vector

Based on the ray theory and Longuet-Higgins’s linear model of sea waves, the joint distribution of wave envelope and apparent wave number vector is established. From the joint distribution, we define a new concept, namely the outer wave number spectrum, to describe the outer characteristics of ocean waves. The analytical form of the outer wave number spectrum, the probability distributions of the apparent wave number vector and its components are then derived. The outer wave number spectrum is compared with the inner wave number spectrum for the average status of wind-wave development corresponding to a peakness factor P = 3. Discussions on the similarity and difference between the outer wave number spectrum and inner one are also presented in the paper.     

Imbalance in the carbonate budget of surficial sediments in the North Atlantic Ocean: variations over the last millenium?

Fluxes contributing to the particulate carbonate system in deep-sea sediments were investigated at the BENGAL site in the Porcupine Abyssal Plain (Northeast Atlantic). Deposition fluxes were estimated using sediment traps at a nominal depth of 3000 m and amounted to 0.37±0.1 mmol C m⁻² d⁻¹. Dissolution of carbonate was determined using flux of total alkalinity from in situ benthic chambers, is 0.4±0.1 mmol C m⁻² d⁻¹. Burial of carbonate was calculated from data on the carbonate content of the sediment and sedimentation rates from a model age based on ¹⁴C dating on foraminifera (0.66±0.1 mmol C m⁻² d⁻¹). Burial plus dissolution was three times larger than particle deposition flux which indicates that steady-state is not achieved in these sediments. Mass balances for other components (BSi, ²¹⁰Pb), and calculations of the focusing factor using ²³⁰Th, show that lateral inputs play only a minor role in this imbalance. Decadal variations of annual particle fluxes are also within the uncertainty of our average. Long-term change in dissolution may contribute to the imbalance, but can not be the main reason because burial alone is greater than the input flux. The observed imbalance is thus the consequence of a large change of carbonate input flux which has occured in the recent past. A box model is used to check the response time of the solid carbonate system in these sediments and the time to reach a new steady-state is in the order of 3 kyr. Thus it is likely that the system has been perturbed recently and that large dissolution and burial rates reflect the previously larger particulate carbonate deposition rates. We estimate that particulate carbonate fluxes have certainly decreased by a factor of at least 3 and that this change has occurred during the last few centuries.     

Method and results of assimilation of climatic data on temperature,salinity, and sea level into a numerical model of the Black Sea

A method for combined assimilation of climatic hydrologic fields of temperature, salinity, and the climatic dynamic level of the Black Sea into a model of sea dynamics is proposed. The monthly mean fields of the dynamic sea level were obtained from the results of assimilation of satellite altimetry data into the model. The statistical characteristics of errors in the forecasts of the level, salinity, and temperature were assumed to be proportional to the statistical characteristics of the differences between monthly mean climatic fields of temperature, salinity, and sea level calculated by means of assimilating altimetry observations of the sea level and analogous climatic hydrologic fields. The climatic fields of currents are reconstructed and analyzed. The assimilation of the climatic altimetry level allows the reproduction (in current fields) of quasi-stationary synoptic anticyclonic eddies located along the periphery of the Black Sea Rim Current.