Freshwater-saltwater interface and estuarine sediment in situ assays based on post-exposure feeding of chironomids and polychaetes

An integrated approach for ecological and ecotoxicological sediment assessments along the freshwater saltwater interface (FSI) and estuarine areas in general was developed, through the adaptation of recently proposed methods for the midge larvaChironomus riparius (an in situ assay based on post-exposure feeding for sediment toxicity assessments in freshwater systems) and the development of new techniques for the polychaeteHediste (Nereis) diversicolor (an in situ post-exposure feeding assay). Based on the previously proposed methods, improvements were made to the feeding quantification ofC. riparius fourth instar larvae. A method to quantify the post-exposure feeding ofH. diversicolor was developed and optimized under laboratory conditions using newly hatchedArtemia franciscana nauplii as a food source. Assay chambers and procedures fromC. riparius assays were easily adapted forH. diversicolor in situ exposures. Sediment assays using this integrated approach were successfully performed at an uncontaminated river-estuarine system. They consisted of a 48-h exposure followed by a 1-h post-exposure feeding for chironomids and a 2-h post-exposure feeding for polychaetes. Good recovery rates were obtained after the 48-h field exposure. Fluctuations in various physicochemical variables at the FSI, due to the tidal regime, did not significantly influenceC. riparius larval post-exposure feeding rates, suggesting that uncontaminated freshwater sites located upstream of FSI areas can be used as reference sites forC. riparius in situ evaluations in estuaries. Adapted assay chambers and procedures were suitable forH. diversicolor in situ exposures.     

Performance evaluation of implicit stochastic reservoir operation optimization supported by long-term mean inflow forecast

This study applies implicit stochastic optimization (ISO) to develop monthly operating rules for a reservoir located in Northeast Brazil. The proposed model differs from typical ISO applications as it uses the forecast of the mean inflow for a future horizon instead of the current-month inflow. Initially, a hundred different 100-year monthly inflow scenarios are synthetically generated and employed as input to a deterministic operation optimization model in order to build a database of optimal operating data. Later, such database is used to fit monthly reservoir rule curves by means of nonlinear regression analysis. Finally, the established rule curves are validated by operating the system under 100 new inflow ensembles. The performance of the proposed technique is compared with those provided by the standard reservoir operating policy (SOP), stochastic dynamic programming (SDP) and perfect-forecast deterministic optimization (PFDO). Different forecasting horizons are tested. For all of them, the results indicate the feasibility of using ISO in view of its lower vulnerability in contrast to the SOP as well as the proximity of its operations with those by PFDO. The results also reveal that there is an optimal choice for the forecasting horizon. The comparison between ISO and SDP shows small differences between both, justifying the adoption of ISO for its simplified mathematics as opposed to SDP.     

High-resolution imaging of sulfur oxidation states, trace elements, and organic molecules distribution in individual microfossils and contempo rary microbial filaments

Owing to the delicate nature of fossil microorganisms and inherent difficulties for discriminating true fossils from artifacts, an important challenge is to extract unequivocal biogenic information from individual microfossils using high-resolution, nondestructive and sensitive techniques. Here, we use combined synchrotron (X-ray microfluorescence, X-ray absorption near-edge structure and infrared microspectroscopies) and particle-induced X-ray emission analyses to image the spatial distribution at a μm-scale of a variety of potential biogenic markers (major and trace elements, C-H bonds, and sulfur-oxidation states) in individual prokaryotic microfossils. In particular, we analyzed iron-oxide fossil filaments of putative biogenic origin encapsulated with amorphous silica from a fragment of an inactive hydrothermal chimney of the East Pacific Rise. In order to test the biogenic origin of the markers studied, we performed the same analyses on filamentous bacteria corresponding most likely to the ?-Proteobacteria, and collected from substrates exposed to a hydrothermal fluid vent at the Mid-Atlantic Ridge. In both types of fossil and contemporary filaments, the occurrence of CH₂ groups and of three sulfur species (sulfate, sulfite, organic S) showing heterogeneous distribution that underline the cytoplasm of individual cells in the case of the present-day filament, suggests that the original microorganisms were actively metabolizing sulfur. These results show the large potential of combining high-resolution synchrotron techniques to analyze individual microfossils for extracting unequivocal biogenic information. Furthermore, they also suggest that cooccurrence of different sulfur oxidation states within single microfossils could constitute a biogenic metabolic marker indicating S-metabolizing activities.     

Patterns of sea surface temperature variability on seasonal to sub-annual scales at and offshore the Río de la Plata estuary

SST variability on seasonal to sub-annual scales in the coastal region of South America between 30° and 39°S, largely influenced by the Rio de la Plata estuary’s plume, and its relation to wind variability are explored. Data are six years of daily ensembles of gridded satellite SST and sea surface winds with spatial resolutions of about 11 and 25 km, respectively. Observations from oceanographic cruises are used to validate the results. It is found that the seasonal cycle can be explained in terms of two modes. The first one, characterizing fall-early winter/spring-early summer, is related to the radiative cycle. The second one, corresponding to late summer and winter, displays warm/cold anomalies along the Uruguayan coast forced by the prevailing winds during those seasons. In the upper estuary and the northern part of the area of influence of the freshwater plume, variability in sub-annual scales is significant. A large portion of this variance is related to zonal wind anomalies that force warm/cold SSTs along that coast. Cold anomalies of up to −5 °C occur under anomalously intense easterly winds, indicating upwelling. These events are very frequent and show large persistence, occurring up to one and a half months. They also display a marked seasonal cycle – being more frequent in late spring and summer – large inter-annual variability and seem to be modulated by the continental runoff. When discharge is low, the freshwater plume retracts to the west, reducing the inner-shelf stratification and increasing the likelihood of a full upwelling to the surface. In winter, short time-scale SST variability is mostly due to variability in the atmospheric cold fronts crossing the region. Weaker or less frequent (stronger or more frequent) fronts produce a generalized warming (cooling) over the region. As the estuary heats (colds) faster than the shelf, a warm (cold) anomaly develops in the upper Río de la Plata. On inter-annual time scales, probably because ENSO activity was weak during the studied period, SST variability was not important.     

Paleohydrological changes in an Amazonian floodplain lake: Santa Ninha Lake

Holocene environments have been reconstructed by sedimentological, mineralogical and organic geochemical analysis of a 270-cm core from Santa Ninha Lake, a floodplain lake in lower Amazonia. Dated by fourteen AMS-radiocarbon dates, the sediment core has a basal age of 5,600?cal?years BP and different sedimentary units were identified. These units document various hydrologic phases in the evolution of this lake. Reduced Amazon River influence, with reduced high-water levels of the river, characterized the period between 5,600 and 5,100?cal?years BP. Comparison with other Amazonian and Andean paleoclimate studies point to a dryer climate during this phase. After 5,100?cal?years BP coarse sediments and quartz increase which suggest a higher inflow of the Amazon River. Between 5,000 and 2,300?cal?years BP the coarse sediments and quartz remain high but the organic carbon showed the lowest values. The riverine inflow caused dilution of the organic material produced in the lake and consequently low rates of carbon flux in these phases were recorded. These results show that the hydrodynamics of the Amazon River strongly influence the behavior, productivity and consequently the sedimentation process in the floodplain lakes.     

Overview and status of estuarine microphytobenthos ecological research in South Africa

This article presents a historical overview of estuarine microphytobenthos (MPB) research in South Africa published over the period 1950 to the present, highlighting major milestones, challenges and estuarine management problems, as well as future research needs within the South African context. The studies that were covered comprise peer-reviewed books and journal articles on relevant research conducted in any estuarine environment during the period reviewed. There was a general increase in MPB research outputs over the decades, from only two publications in the 1950s, to over 20 outputs between 2010 and the present. Whereas the MPB studies in South Africa cover a broad spectrum of themes, the research priorities in these works have changed from taxonomically biased studies to those of ecologically based research. Research in the 1950s and 1960s was exclusively taxonomic in nature, with the first ecological investigation incorporating MPB being produced in the 1970s. By the 1980s, ecological studies dominated the research outputs and this trend has persisted to the present. The 1990s and 2000s saw the rise of research into the role of fresh water as a driver of MPB dynamics, whereas the period 2010 to the present saw an increase in more diverse ecological themes, ranging from an autecological investigation to food-web studies and the assessment of multiple drivers of MPB dynamics. However, the majority of studies have focused on either diatoms or estimates of overall MPB biomass. Moreover, there is a regional underrepresentation that runs broadly along biogeographic lines, with the bulk of the work having been conducted in the warm-temperate and subtropical zones of South Africa. Challenges and future research needs for the region are outlined, as is the need to expand MPB research to include other aspects of the biology and ecology of this flora.     

How do management alternatives of fast-growing forests affect water quantity and quality in southeastern Brazil? Insights from a paired catchment experiment

Fast-growing forest plantations have been expanding in Brazil in the last 50 years, which reach productivities by over 40 m³ ha⁻¹ year⁻¹ in reduced rotation between 5 and 15 years. In the 1990s, environmental warnings about these plantations guided research projects seeking to understand their effects on water and propose forest management actions to minimize them. The assessment of forest management effects on water resources is conducted by long-term experiments in paired catchments. In this paper we present results of some studies conducted at the hydrological monitoring centre of Itatinga Experimental Forest Station, of the University of São Paulo, where hydrological monitoring began in 1987, and currently include three catchments (83–98 ha) under different forest management regimes: short-rotation Eucalyptus plantation, long-term forest plantation mosaic and native forest restoration. Results show that at similar conditions observed at study area including deep soils and good natural water regulation, hydrological effects vary according to the forest management regime adopted, increasing water consumption and making the flow regime vulnerable to intra- and inter-annual seasonality. Regarding water quality, weekly sampling results showed suspended sediments and nitrate concentrations below water quality thresholds criteria by silvicultural operations, and the effects were transient but higher concentrations of nutrients were observed in intensive management regime. In the study area, reducing the management intensity of forest plantation by increasing the rotation time, adopting forest age mosaic and avoiding the coppice technique are alternative choices that reduced water use and increased flow regulation. Different adopted forest management schemes directly affected water use, showing that in water-deficit tropical regions, management regime of fast-growing forest plantations controls water availability.     

Towards a framework for geospatial tangible user interfaces in collaborative urban planning

The increasing complexity of urban planning projects today requires new approaches to better integrate stakeholders with different professional backgrounds throughout a city. Traditional tools used in urban planning are designed for experts and offer little opportunity for participation and collaborative design. This paper introduces the concept of geospatial tangible user interfaces (GTUI) and reports on the design and implementation as well as the usability of such a GTUI to support stakeholder participation in collaborative urban planning. The proposed system uses physical objects to interact with large digital maps and geospatial data projected onto a tabletop. It is implemented using a PostGIS database, a web map server providing OGC web services, the computer vision framework reacTIVision, a Java-based TUIO client, and GeoTools. We describe how a GTUI has be instantiated and evaluated within the scope of two case studies related to real world collaborative urban planning scenarios. Our results confirm the feasibility of our proposed GTUI solutions to (a) instantiate different urban planning scenarios, (b) support collaboration, and (c) ensure an acceptable usability.     

Trophic transfer of methylmercury and trace elements by tropical estuarine seston and plankton

Methylmercury (MeHg) and trace elements (TE), mercury, selenium, cadmium, lead and copper, were determined in a microbial loop composed by three size classes of autotrophic and heterotrophic microorganism samples, 1.2–70 μm (seston, SPM), 70–290 μm (microplankton) and ≥290 μm (mesoplankton) from five sampling stations within a polluted eutrophic estuary in the Brazilian Southeast coast and one external point under the influence of the bay. TE concentrations were within the range reported for marine microorganisms from uncontaminated locations. Microplankton was primarily composed of proto-zooplankton and diatoms (>90%) while approximately 50% of mesoplankton was composed mainly of copepods. MeHg and TE in samples did not differ among the five sampling stations within the bay. Cd, Pb and Cu in seston were higher in the stations sampled inside Guanabara Bay (0.67 μg Cd g⁻¹, 9.26 μg Pb g⁻¹, 8.03 μg Cu g⁻¹) than in the external one (0.17 μg Cd g⁻¹, 3.98 μg Pb g⁻¹ and 2.09 μg Cu g⁻¹). Hg, MeHg and Se did not differ among the five points within the more eutrophic waters of the estuary and the external sampling station. The trophic transfer of MeHg and Se was observed between trophic levels from prey (seston and microplankton) to predator (mesoplankton). The successive amplification of the ratios of MeHg to Hg with increasing trophic levels from seston (43%), to microplankton (59%) and mesoplankton (77%) indicate that biomagnification may be occurring along the microbial food web. Selenium, that is efficiently accumulated by organisms through trophic transference, was biomagnified along the microbial food web, while Hg, Cd, Pb, Cu did not present the same behavior. Concentrations differed between the three size classes, indicating that MeHg and TE accumulation were size-dependent. MeHg and TE concentrations were not related to the taxonomic groups' composition of the planktonic microorganisms. Results suggest the importance of the role of the trophic level and microorganism size in regulating element transfers. Eutrophication dilution may provide a process-oriented explanation for lower MeHg and TE accumulation by the three size classes of microorganisms collected at the five sampling stations within the bay.