Habitability analyses of aquatic bacteria

Habitability is defined as an ability of an organism to inhabit different environments. Habitability of organisms, however, cannot be inferred from analyses such as a whole genome or community structures. A recently developed database, the MetaMetaDB, gives us information from what kind of environments one particular 16S rRNA sequence data has ever been obtained, and thus enables us to infer the habitability of the bacterium in question. In order to check the applicability of this database to study the habitability of aquatic bacteria, samples collected at two Naka River stations, one estuarine station from Naka River Estuary, two coastal stations at Oarai in Ibaraki Prefecture, Japan and one station in the Kuroshio Current of the western North Pacific were examined. The phylotypes were tracked against the MetaMetaDB and it was reasonably found that the low-salinity stations were dominated by sequences with “freshwater and groundwater”, “human” and “wastewater” habitat identities, while the high-salinity stations were dominated by those with a “marine” identity. The phylotypes of low-salinity stations with a particular habitat identity were absent or rare in the high-salinity stations and vice versa. The MetaMetaDB also showed that sequences of Cyanobacteria or related phylogenetic groups may be present in the human gut, as well as the probable distribution of the relatives (ancestors/descendants/siblings) of some bacteria. These overall findings proved that the MetaMetaDB is useful as a new tool to infer microbial habitability and it gives us new information on the possible origin and ecology of microorganisms in the environments.     

BALLOON-BORNE MEASUREMENTS OF AEROSOL VERTICAL DISTRIBUTIONS OVER BEIJING DURING THE SUMMER AND AUTUMN OF 1993

The results of two balloon soundings during the summer and autumn of 1993 from the Xianghe Observation Station are being utilized in a study of the temporal and spatial distribution of the atmospheric aerosols.The balloon,gondola,instrumentation and atmospheric conditions during the observation period are described.The temporal and spatial characteristics of aerosol concentration,size ratio,mixing ratio,and size distribution for both troposphere and strato-sphere are presented and analyzed.     

A model of fish preference and mortality under hypoxic water in the coastal environment

Eutrophication and associated occurrence of hypoxic condition could cause significant damage to marine ecosystems, resulting in considerable economic losses to fisheries and aquaculture and is a major source of stress that fish often have to contend with in order to survive. This problem is likely to be exacerbated in the coming years, since the wastewater treatment facilities is unlikely to catch up with increasing human activities. Moreover, large-scale reclamation projects in coastal areas have recently been increased, and these activities certainly have adverse impacts on water quality and fisheries resources. Coastal construction has a significant role in the development of hypoxic water by changing the current and mixing pattern of water. Changes in species composition and decreases in species richness and diversity have been well documented in hypoxic systems. Hypoxia could cause endocrine disruption in fish and eliminate populations of sensitive species. Shallow coastal areas are of great importance for the special nursery of fish and shellfish and land reclamation in these areas cause strong damage to fisheries. Although the tolerance of aquatic life to hypoxia is known, there is no information about the mortality of fish caused by hypoxia because fish can swim around it and no modeling study has yet been carried out. Criteria that influence the movement of fish are: amounts of food, water temperature and depth, dissolved oxygen concentration and nature of seabed. However, among these, water temperature and dissolved oxygen are the most crucial parameters that affect survival, movement and growth of fish. In this paper, a model of fish preference and mortality for environmental conditions was developed and applied to the Hakata Bay where hypoxic water occurs every summer. For the purpose of this study, a field survey of fish behavior under hypoxic water was conducted by releasing marbled sale (Pleuronectes yokohamae) in the inner bay. Moreover, a series of preference tests for DO, salinity and temperature in the laboratory were conducted in order to decide preference parameters of fish. Using the results of both field and laboratory studies, a sub-module of fish preference and mortality was coded within an integrated hydrothermal and eutrophication model (CHEM) to predict the behavior and mortality of marbled sale when hypoxia would occur in the bay. The model could reasonably simulate the behavior of the fish under hypoxia. An assessment of the impact of the ongoing land reclamation project of about 401 ha in the Wajiro tidal flat zones at the head of the bay on the fisheries resources was also conducted using the model. The results showed that the artificial land lowered the mortality rate of fish under hypoxic condition in the bay during the summer period.     

Ice-silicate fractionation among icy bodies due to the difference of impact strength between ice and ice-silicate mixture

Laboratory experiments on the impact disruption of ice-silicate mixtures were conducted to clarify the accretion process of small icy bodies. Since the icy bodies are composed of ice and silicates with various porosities, we investigated the effect of porosity on the impact disruption of mixtures. We tested the mixture target with the mass ratio of ice to silicate, 0.5 and with 5 different porosities (0, 12.5, 25, 32, 37%) at the impact velocities of 150 to 670 m/s. The silicate mass ratio was changed from 0 to 0.5 in steps of 0.1 at a porosity of 12.5% and a constant impact velocity of about 300 m/s. The impact strength of the mixture was found to decrease with increasing porosity and the silicate mass ratio between 0.1 and 0.5 could enhance the strength of the icy target. The observed dependence of the impact strength on the porosity is opposite to that observed for pure ice. This difference could play an important role in ice-silicate fractionation during the accretion process. Because, ice rich bodies are easily broken as the porosity decreases in their evolution, the collisional growth could be prohibited. On the other hand, among the silicate rich bodies the collisional growth could be enhanced.     

Microbial responses using denaturing gradient gel electrophoresis to oil and chemical dispersant in enclosed ecosystems

Microbial responses to the addition of oil with or without a chemical dispersant were examined in mesocosm and microcosm experiments by using denaturing gradient gel electrophoresis of bacterial ribosomal DNA and direct cell counting. When a water-soluble fraction of oil was added to seawater, increases in cell density were observed in the first 24h, followed by a decrease in abundance and a change in bacterial species composition. After addition of an oil-dispersant mixture, increases in cell density and changes in community structure coincided, and the amount of bacteria remained high. These phenomena also occurred in response to addition of only dispersant. Our results suggest that the chemical dispersant may be used as a nutrient source by some bacterial groups and may directly or indirectly prevent the growth of other bacterial groups.     

Spin rates of fast-rotating asteroids and fragments in impact disruption

We present a new experimental result of fragment spin-rate in impact disruption, using a thin glass plate. A cylindrical projectile impacts on a side (edge) of the plate. Dispersed fragments are observed using a high-speed camera and the spin rates of fragments are measured. We find that the measured fragment spin-rate decreases with increasing size. Assuming that the rotational energy of fragments is supplied from the residual stress, the spin rate ω decreases with increasing fragment size r as ω∼r−1, which explains the above experimental results. This size-dependence is similar to that of the observed spin rates of small fast-rotating asteroids. Our results suggest that spin rates of fragments of small asteroids immediately after disruption may have a similar size-dependence, and can provide constraints on the subsequent spin-state evolution of small asteroids due to thermal torques.     

Low-velocity collisions between centimeter-sized snowballs: Porosity dependence of coefficient of restitution for ice aggregates analogues in the Solar System

Understanding the collisional behavior of ice dust aggregates at low velocity is a key to determining the formation process of small icy bodies such as icy planetesimals, comets and icy satellites, and this collisional behavior is also closely related to the energy dissipation mechanism in Saturn’s rings. We performed head-on collision experiments in air by means of free-falling centimeter-sized sintered snowballs with porosities from 44% to 80% at impact velocities from 0.44 m s^?1 to 4.12 m s^?1 at ?10 °C. In cases of porosity larger than 70%, impact sticking was the dominant collision outcome, while bouncing was dominant at lower porosity. Coefficients of restitution of snow in this velocity range were found to depend strongly on the porosity rather than the impact velocity and to decrease with the increase of the porosity. We successfully measured the compaction volume of snowballs after the impact, and it enabled us to estimate the dynamic compressive strength of snow with the assumption of the energy conservation between kinetic energy and work for deformation, which was found to be consistent with the upper limit of static compressive strength. The velocity dependence of coefficients of restitution of snow was analyzed using a Johnson’s model, and a diagram for collision outcomes among equal-sized sintered snowballs was successfully drawn as a function of porosity and impact velocity.     

New Late Jurassic palaeomagnetic data from the northern Sichuan basin: implications for the deformation of the Yangtze craton

Upper Jurassic red sandstones and red siltstones were collected from 67 layers at 12 localities in the Penglaizhen formation. This formation is in the north of Bazhong county (31.8°N, 106.7°E) in the Sichuan basin, which is located in the northern part of the Yangtze craton. Thermal demagnetization isolated a high-temperature magnetic component with a maximum unblocking temperature of about 690 °C from 45 layers. The primary nature of the magnetization acquisition is ascertained through the presence of magnetostratigraphic sequences with normal and reversed polarities, as well as positive fold and reversal tests at the 95 per cent confidence level. The tilt-corrected mean direction of 36 layers is D = 20.0°, I = 28.8° with α ₉₅ = 5.8°. A Late Jurassic palaeomagentic pole at 64.7°N, 236.0°E with A ₉₅ = 7.0° is calculated from the palaeomagnetic directions of 11 localities. This pole position agrees with the two other Late Jurassic poles from the northern part of the Yangtze craton. A characteristic Late Jurassic pole is calculated from the three poles (68.6°N, 236.0°E with A ₉₅ = 8.0°) for the northern part of the Yangtze craton. This pole position is significantly different from that for the southern part of the Yangtze craton. This suggests that the southern part of the Yangtze craton was subjected to southward extrusion by 1700 ± 1000  km with respect to the northern part. Intracraton deformation occurred within the Yangtze craton.     

Removal of water‐surface reflection effects with a temporal minimum filter for UAV‐based shallow‐water photogrammetry

The recent development of structure‐from‐motion (SfM) and multi‐view stereo (MVS) photogrammetry techniques has enabled semi‐automatic high‐resolution bathymetry using aerial images taken by consumer‐grade digital cameras mounted on unmanned aerial vehicles (UAVs). However, the applicability of these techniques is sometimes limited by sun and sky reflections at the water surface, which render the point‐cloud density and accuracy insufficient. In this research, we present a new imaging technique to suppress the effect of these water‐surface reflections. In this technique, we order a drone to take a short video instead of a still picture at each waypoint. We then apply a temporal minimum filter to the video. This filter extracts the smallest RGB values in all the video frames for each pixel, and composes an image with greatly reduced reflection effects. To assess the performance of this technique, we applied it at three small shallow‐water sites. Specifically, we evaluated the effect of the technique on the point cloud density and the accuracy and precision of the photogrammetry. The results showed that the proposed technique achieved a far denser point cloud than the case in which a randomly chosen frame was used for each waypoint, and also showed better overall accuracy and precision in estimating water‐bottom elevation. The effectiveness of this new technique should depend on the surface wave state and sky radiance distribution, and this dependence, as well as the applicability to large areas, should be investigated in future research. Copyright © 2018 John Wiley & Sons, Ltd.