Effects of benzene and dimethylnaphthalene on homeostatic processes in two species of crabs

Long-term exposure of animals to sub-lethal doses of toxicants such as benzene (B) and dimethylnaphthalene (DMN) may result in subtle changes in their physiology and biochemistry. In crustaceans such changes include decreased rate of limb regeneration, extended time to molt and decreased growth increment at molt.^1,2 These processes depend upon an adequate supply of stored nutrients in the tissues and appropriate release of neuroendocrine substances from the central nervous system.^3,4 We are examining the effects of sub-lethal doses of B and DMN on osmotic and ionic regulation and on nutrient storage in the blue crab, Callinectes sapidus, and the green crab, Carcinus maenas. Disturbances in these physiological processes may reflect alterations in neuroendocrine functions brought about by the presence of toxicants. We find that exposure of C. maenas to B or DMN, followed by transfer to a dilute medium, results in impaired osmoregulatory capacity, while addition of these compounds to a dilute medium to which the animal is already acclimated does not affect their regulatory ability. Storage of carbohydrate in the midgut gland (hepatopancreas) is decreased by B or DMN, while the accessory storage tissues (gill, muscle) are not significantly affected.     

Land-derived pollutant hydrocarbons

Evidence is given for the derivation of oil pollution from on-shore sources and for a possible relation between its concentration in estuarine sediment and in the lipids of the sediment biomass.     

A system for large volume aerosol sampling in the stratosphere using electrostatic precipitation

Summary An instrumental system for securing aerosol samples in the stratosphere by electrostatic precipitation has been developed. The system is designed to be balloon borne to altitudes between 30 km and 45 km and to collect with high efficiency the aerosol content of the stratosphere at these altitudes. Volumetric sampling rates of one meter³ sec^–1 ambient air are obtained with the sampling configuration with collection efficiency in excess of 95 percent for particles between .01 microns (mmd) and 2 microns (mmd).Specially developed associated instrumentation for altitude and air flow measurement points to an important versatility in the application of gas discharges for low-pressure (or high altitude) altimetry and anemometry over a very wide range of air densities and tempertures.The theory, operation, and performance of the aerosol sampling system and associated subsytems are described. Certain new observations of low-density corona physics phenomena are reported and potential applications to other areas of research and technology are indicated.

---

Zusammenfassung Es wurde ein Instrument entwickelt, mit dem man Aerosolproben aus der Stratosphäre durch elektrostatischen Niederschlag erhalten kann. Das System wird von einem Ballon in Höhen zwischen 30 und 45 km getragen und sammelt mit hoher Wirksamkeit Proben des Aerosolgehalts in diesen Höhen. Mit dem Instrument wird eine Sammelgeschwindigkeit von 1 m³/sek erreicht bei einer Sammelwirksamkeit von mehr als 95% für Partikel von Größen zwischen 0,01 und 2 Mikron.Besonders entwickelte angeschlossene Instrumente zur Messung der Höhe und der Luftströmungen zeigen die wesentliche Vielseitigkeit der Anwendung von Gasentladungen für die Höhenmessung und Windmessung bei niedrigem Druck (oder in großen Höhen) in einem großen Bereich der Luftdichte und der Temperatur.Es werden die Theorie, die Arbeitsweise und die Ausführung des Aerosolprobeninstrumentes und der angeschlossenen Hilfssysteme beschrieben. Es wird über einige neue Beobachtungen aus dem Bereich der Phänomene der Gasentladungen bei niedriger Dichte berichtet und neue mögliche Anwendungen in anderen Zweigen der Forschung und Technologie werden angegeben.

---

Résumé On a construit un instrument au moyen duquel on peut obtenir des échantillons d'aérosole stratosphérique par précipitation électrostatique. Le système est emporté par un ballon à des altitudes de 30 à 45 km et rassemble avec efficacité des echantillons du contenu de l'aérosole à ces altitudes. Avec cet appareil, on obtient une vitesse de prise de 1 m³/sec avec une efficacité de plus de 95% pour les particules comprises entre 0,01 et 2 microns.Des appareils spéciaux annexes permettent de mesurer la hauteur et les courants d'air. Ils démontrent les possibilités multiples de l'utilisation de la décharge dans un gaz pour la mesure de l'altitude et des vents par pression très faible (ou à très haute altitude) dans un domaine étendu tant de la densité de l'air que de la température.On décrit dans ce mémoire la théorie, le mode de travail et l'exécution de cet instrument de prise d'échantillons d'aérosoles ainsi que des systèmes auxiliaires annexes. On rapporte sur quelques nouvelles observations faites dans le domaine des phénomènes connexes à la décharge dans un gaz par faible densité. On indique en outre de nouvelles possibilités d'utilisation du système dans d'autres branches de la recherche ou de la technologie.

---

---

With 16 Figures

---

This work has been supported by the Division of Biology and Medicine, U. S. Atomic Energy Commission, under contract at (30-1)-2363 with Del Electronics.     

Genesis and solvus relations of submicroscopically intergrown paragonite and phengite in a blueschist from northern California

Electron microbeam techniques have been used to examine submicroscopically intergrown paragonite, phengite and chlorite from the South Fork Mountain Schist of the Franciscan Terrane of northern California, which was subjected to blueschist facies metamorphism. The sample also contains quartz, albite, lawsonite, and rutile. The subassemblage albite-lawsonite-rutile requires metamorphic conditions on the low-temperature side of the equilibrium albite+lawsonite+rutile=paragonite+sphene+quartz+H₂O (T<200° C and P<7.4 kbars based on thermodynamic data of Holland and Powell 1990). The white micas appear to be optically homogeneous, but back-scattered electron images can distinguish two different micas by their slight difference in contrast. Electron microprobe analyses (EMPA) of micas show Na/(Na+K) ranging from 0.2 to 0.8. The two micas are resolved by transmission electron microscopy (TEM) as packets of phengite and paragonite that range from 20 to several hundred nm in thickness. The compositions, determined by analytical electron microscopy (AEM), constrain the limbs of the phengite-paragonite solvus to values of Na/(Na+K)=<0.02 and 0.97, representing less mutual solid solution than ever reported by EMPA. The textural relations imply that the sheet silicates were derived from reactions between fluids and detrital clays and that they are in an intermediate stage of textural development. We caution that microprobe analyses of apparently homogeneous sheet silicates may yield erroneous data and lead to faulty conclusions using phengite barometry and paragonite-muscovite thermometry, especially in fine-grained rocks that formed at relatively low temperatures. Contribution no. 473 from the Mineralogical Laboratory, Department of Geological Sciences, The University of Michigan, Ann Arbor, Michigan, USA     

Groundwater quality assessment from a hard rock terrain, Salem district of Tamilnadu, India

A total of 162 groundwater samples for three representative seasons were collected from Salem district of Tamilnadu, India to decipher hydrogeochemistry and groundwater quality for determining its suitability for drinking and agricultural proposes. The water is neutral to alkaline in nature with pH ranging from 6.6 to 8.6 with an average of 8.0. Higher electrical conductivity was observed during post-monsoon season. The abundance of major ions in the groundwater was in the order of $ {\text{Na} > \text{Ca} > \text{Mg} > \text{K} = \text{Cl} > \text{HC}}{{\text{O}}_3}\; > \;{\text{S}}{{\text{O}}_4}\; > \;{\text{N}}{{\text{O}}_3} $ . Piper plot reveals the dominance of geochemical facies as mixed Ca–Mg–Cl, Na–Cl, Ca–HCO₃, Ca–Na–HCO₃, and Ca–Cl type. NO₃, Cl, SO₄, and F exceed the permissible limit during summer and post-monsoon seasons. Sodium adsorption ratio was higher during post-monsoon and southwest monsoon season indicating high and low salinity, satisfactory for plants having moderate salt tolerance on soils. Permeability index of water irrespective of season falls in class I and class II indicating water is moderate to good for irrigation purposes. As per the classification of water for irrigation purpose, water is fit for domestic and agricultural purposes with minor exceptions irrespective of seasons.     

Study of a rockfall in a limy conglomerate canyon (Covarrubias, Burgos, N. Spain)

In this paper, a rockfall of some tenths of cubic metres of rock, occurred in a conglomerate canyon in the northern Castilian plateau (N-Central Spain), is described in detail. This includes the basic data (geology, mapping, meteorological data and rock involved), the mechanical mechanism of the instability, the propagation and reach of the fallen rock boulders and the final location of the pieces. The study is based on detailed geological, aerial photography and geotechnical studies and advance topographical measurements. Geological engineering techniques are used to analyze the instability initiation and rockfall propagation. This study contributes to a better understanding of the geological processes leading to the occurrence of calcareous canyons and cliffs in the drainage network of the upper Castilian plateau. It is also helpful to improve our knowledge on natural rockfall phenomena for risk engineering purposes aiming to quantify, control and mitigate associated hazards.     

Seasonal cycle of surface circulation in the coastal North Indian Ocean

Monthly-mean winds and currents have been used to identify the driving mechanisms of seasonal coastal circulation in the North Indian Ocean. The main conclusions are: (i) the surface circulation off Arabia is typical of a wind-driven system with similar patterns of longshore current and wind stress; (ii) circulation off the west coast of India is consistent with the dynamics of a wind-driven eastern boundary current only during the southwest monsoon. During the northeast monsoon it is possible that the influence of the interior flow is important. (iii) There are at least three mechanisms that influence the surface circulation off the east coast of India: wind-stress, influence of fresh-water run off and contribution of the interior flow. It is difficult at present to assess the relative importance of these three processes.     

Contrasting rifted margin styles south of Greenland: implications for mantle plume dynamics

We present new and reprocessed seismic reflection data from the area where the southeast and southwest Greenland margins intersected to form a triple junction south of Greenland in the early Tertiary. During breakup at 56 Ma, thick igneous crust was accreted along the entire 1300-km-long southeast Greenland margin from the Greenland Iceland Ridge to, and possibly 100 km beyond, the triple junction into the Labrador Sea. However, highly extended and thin crust 250 km to the west of the triple junction suggests that magmatically starved crustal formation occurred on the southwest Greenland margin at the same time. Thus, a transition from a volcanic to a non-volcanic margin over only 100–200 km is observed. Magmatism related to the impact of the Iceland plume below the North Atlantic around 61 Ma is known from central-west and southeast Greenland. The new seismic data also suggest the presence of a small volcanic plateau of similar age close to the triple junction. The extent of initial plume-related volcanism inferred from these observations is explained by a model of lateral flow of plume material that is guided by relief at the base of the lithosphere. Plume mantle is channelled to great distances provided that significant melting does not take place. Melting causes cooling and dehydration of the plume mantle. The associated viscosity increase acts against lateral flow and restricts plume material to its point of entry into an actively spreading rift. We further suggest that thick Archaean lithosphere blocked direct flow of plume material into the magma-starved southwest Greenland margin while the plume was free to flow into the central west and east Greenland margins. The model is consistent with a plume layer that is only moderately hotter, 100–200°C, than ambient mantle temperature, and has a thickness comparable to lithospheric thickness variations, 50–100 km. Lithospheric architecture, the timing of continental rifting and viscosity changes due to melting of the plume material are therefore critical parameters for understanding the distribution of magmatism.     

Effects of water-borne copper on metallothionein and lipid peroxidation in the marine amphipod Gammarus locusta

The aim of the current work was to determine over 10 days the effects of water-borne exposure of the marine amphipod Gammarus locusta to 4 microgCu l(-1) on the metallothionein (MT; measured by differential pulse polarography) protection system and lipid peroxidation (LP: thiobarbituric acid-reactive malondialdehyde equivalents) as a measure of oxidative damage. MT levels in exposed animals increased significantly at day 2 (36% > control; P < 0.001) and remained high at days 6 and 10 (55 and 38%, respectively, P < 0.001). The maximum level of MT at day 6 coincided with the highest Cu body-burden. LP increased within I day of exposure, indicative of Cu as an oxidative stressor. However, in contrast to MT, the highest LP level was seen at day 4 (68% > control, P < 0.001) before returning to control values by day 6, indicating a protective role of MT against the pro-oxidant effects of Cu.