Metabolism of perylene by monooxygenases of the skin and liver of Ambystoma tigrinum

The prevalence of dermal neoplasia among neotenic tiger salamanders (Ambystoma tigrinum) collected from the Reese Air Force Base sewage lagoon far exceeded that among salamanders from nearby uncontaminated lagoons. Perylene was the predominant pglycyclic aromatic hydrocarbon contaminant in this lagoon. Perylene was shown to be a substrate for monooxygenases in skin and liver homogenates and formed DNA-binding metabolites. Perylene exposure appeared to augment subsequent perylene metabolism by dermal enzymes but it was not a strong inducer of hepatic monooxygenases. Aroclor 1254 induced benzo(a)pyrene and perylene metabolism by both skin and liver enzymes. However, perylene apparently was not activated by salamander enzymes, as suggested by its lack of in vitro mutagenicity and failure to induce tumors.     

Using the Mellor–Yamada mixing scheme in seasonally ice-covered seas—Corrigendum to: Parameterization of vertical mixing in the Weddell Sea [Ocean Modelling 6 (2004) 83–100]

A coding error in the s-Coordinate Primitive Equation Model (SPEM) has led to misleading statements about the behaviour of the Mellor–Yamada level 2 parameterization of vertical mixing. It has been claimed that the scheme removes static instability only very slowly and preserves statically unstable stratifications for an unrealistic long time. This note corrects this statement by demonstrating that the Mellor–Yamada mixing scheme, if implemented correctly, tends to overestimate rather than underestimate vertical mixing in seasonally ice-covered seas. Similar to other mixing schemes with the same behaviour, this leads to spurious open ocean deep convection, an unrealistic homogenization of the water column, and a significant reduction of sea ice volume.     

Cyclone and bioturbation effects on sediments from coral reef lagoons

Despite the severity of tropical cyclone ‘Winifred’, which crossed the Great Barrier Reef on 1 February 1986, there were little long-term effects on lagoon surface sediments from reefs in its path. Short-term effects were apparent only at one particularly exposed area. These were: an increase in proportion of the coarse fraction, the establishment of sand ripples, and the destruction of the mounds produced by callianassid shrimps (normally the dominant topographic feature). Within six weeks this area was indistinguishable from a typical reef lagoon. This is probably the result of sediment reworking by callianassid shrimp, involving selective burial of the coarse fragments and transport to the surface of finer particles. Sediment turnover rates by callianassids are commensurate with change to the sediment within the relatively short period observed. The sediment fauna responded quickly to the changes in sediment type. Immediately after the cyclone the disturbed area supported a fauna typical of the coarse sediments on the shallow reef flat, as the sediment reverted to a more normal type so the fauna changed back to that typical of a reef lagoon.     

On the structural evolution of the Central Trough in the Norwegian and Danish sectors of the North Sea

The Central Trough of the North Sea is not a simple rift graben. It is an elongated area of regional subsidence which was initiated in mid Cretaceous times and continued to subside through to the late Tertiary. Its form is not representative of pre-mid Cretaceous tectonics.In Late Permian times the North Sea was divided into a northern and southern Zechstein basin by the E-W trending Mid North Sea-Ringkøbing-Fyn High. The latter was dissected by a narrow graben trending NNW through the Tail End Graben and the Søgne Basin. The Feda Graben was a minor basin on the northern flank of the Mid North Sea High at this time. This structural configuration persisted until end Middle Jurassic times when a new WNW trend separated the Tail End Graben from the Søgne Basin. Right lateral wrench movement on this new trend caused excessive subsudence in the Tail End and Feda Grabens while the Søgne Basin became inactive.Upper Jurassic subsidence trends continued during the Early Cretaceous causing the deposition of large thicknesses of sediments in local areas along the trend. From mid Cretaceous times the regional subsidence of the Central Trough was dominant but significant structural inversions occurred in those areas of maximum Early Cretaceous and Late Jurassic subsidence.     

Relative molar mass distributions of chromophoric colloidal organic matter in coastal seawater determined by Flow Field-Flow Fractionation with UV absorbance and fluorescence detection

A new method for the characterization of chromophoric colloidal organic matter in seawater has been applied to samples from the Baltic Sea, Kattegatt and Skagerrak seas. Size fractionation of the sample by Flow Field-Flow Fractionation and measurement of the fluorescent and UV absorbing properties of the individual size fractions result in a relative molar mass distribution (RMM) of the optical properties. The RMM distributions have been used to estimate number and weight average relative molar masses, and polydispersity indices. At least two sources of coloured organic matter were identified from the ratio of fluorescence to UV: the Baltic surface water and the Skagerrak deep water. The dominating processes were mixing and dilution, but processes such as photo bleaching of fluorescence are also believed to be important. The RMM distribution derived from UV detection (1150–1300 Dalton) increased with increasing salinity while that derived for fluorescence (1500–1250 Dalton) decreased with increasing salinity. The specific UV absorbance taken as a proxy of the aromaticity of the chromophoric organic material showed decreasing trend with both increasing salinity and increasing UV derived weight average relative molar mass. Increasing polydispersity of the colloidal material was also observed as a function of salinity.     

Mapping sediment acoustic impedance using remote sensing acoustic techniques in a shallow-water carbonate environment

 Real-time trackline plots of surficial sediment acoustic impedance delineate several sedimentary facies off Garden Key in the Dry Tortugas. The sea floor within a 6×6 km surveyed area consists of carbonate muds (silts), sands and shell, rock, and live corals. The 4-kHz acoustic data supports this finding by providing a pictographic representation of the distribution and structure of several sediment facies types. Plotting the gridded acoustic data with commercial mapping software (Surfer) provides a three-dimensional (3D) perspective of the bottom topography with a color contour map of surficial sediment impedance (upper 0.4 m) draped over the 3D surface.