东海黑潮表层盐度分布特征及其影响因素

利用美国国家海洋大气管理局2007年发布的全球海域温、盐数据库资料,美国地球物理数据中心2006年发布的海底地形数据库资料以及日本海洋科学与技术机构2003年发布的1997—2002年东海地区月平均降水量资料,研究东海黑潮表层盐度的月季分布特征,并分析其影响因素。结果表明,东海黑潮表层盐度存在明显的月季变化特征。总体而言,12月至次年3月表层盐度高,6—9月表层盐度低,4、5月和10、11月为过渡阶段;表层盐度高值分布在东海黑潮主段靠近东边界一侧;6—9月入口段的表层盐度高于出口段的表层盐度,其他月份入口段的表层盐度低于出口段的表层盐度。东海黑潮表层盐度主要受表层温度、降水、径流的影响。冬、春、秋季的表层盐度分布在黑潮主段靠近陆架一侧区域受表层温度影响大;降水对东海黑潮表层盐度产生局部小范围的影响,时间主要集中在1月和6—8月份,区域分布在低纬25°N以南和30°N附近。长江冲淡水夏季对东海黑潮表层盐度的影响大于其他季节对东海黑潮表层盐度的影响,7月长江径流量达到最大值时,对应的黑潮扇形区的盐度最低。     

Prediction of Extreme Sea-State Encountered by Oceangoing Vessels

In order to determine the extreme sea-state encountered by oceangoing vessels,a method forthe prediction of wave parameters based on the data of long-term distribution sample is developed by useof the fitting approach with the Weibull probability functions and the power functions.As an example aspecial calculation result is given including the analysis of wave parameters for the data of the NorthAtlantic Basin and the computation of motion and load encountered by two container ships CSLR andCSBV in different loading conditions.All computation results are satisfactory compared with corre-sponding design results.     

四川冕宁稀土矿床黑色风化物中的稀土元素赋存状态研究

本文用现代的固体表面分析方法和连续选择性提取法对冕宁黑色风化物中稀土元素的赋存状态进行研究因。在可交换离子相,易还原相,水成相和矿物结晶相四种稀土存在的形式中,易还原相和水成相是自然界矿物中新的稀 存状态。特性吸附是产生这两种新相的内在原因。     

Characteristics of Upper Mantle Activity in the South China Sea Region and the Indochina Mantle Plume

According to computed results of the mantle traction field beneath the lithosphere based on satellite-modelled gravity anomalies of different degrees, it has been revealed that the three types of mantle convection on different scales existing in the South China Sea region is the key factor controlling the geodynamics. The mantle convection models on large and middle scales have been proved by natural seismic S-wave tomographic data and interpreted by using the present mantle plume concept In consideration of other relevant geological and geophysical data, the authors put emphasis on discussing the expression form, origin and age of the Indochina mantle plume and its important effect on the conversion of organic matter and hydrocarbon accumulation in Cenozoic basins.     

Response Characteristics of Load on Vessels in Waves

Considering the requirement of direct design and fatigue test for ships and floating structures byuse of FEM technique,a computational procedure of spectral analysis for wave load on the hull surface is de-veloped in this paper.The response of hydrodynamic pressure on the body surface to a designated sea state forships and floating structures is calculated by use of the revised strip method with the hull bound perturbationflow concept.The spectral function of wave load for the defined point on the body surface can be determinedfrom the Wiener-Khinhin theorem and the characteristic load value can be also obtained from spectral mo-ment analysis.A container ship is taken as a computational example and the sample of wave load with a cer-tain probability and corresponding encountered frequency is provided.     

Identification of Prorocentrum minimum and Takayama pulchella by fluorescence in situ hybridization through epifluorescence microscopy and flow cytometry

Partial rDNA sequences of Prorocentrum minimum and Takayama pulchella were amplified, cloned and sequenced, and these sequence data were deposited in the GenBank. Eight oligonucleotide probes (DNA probes) were designed based on the sequence analysis. The probes were employed to detect and identify P. minimum and T. pulchella in unialgal and mixed algal samples with a fluorescence in situ hybridization method using flow cytometry. Epifluorescence micrographs showed that these specific probes labeled with flu...     

Inheritance of 15 microsatellites in the Pacific oyster Crassostrea gigas： segregation and null allele identification for linkage analysis

Microsatellites were screened in a backcross family of the Pacific oyster, Crassostrea gigas. Fifteen microsatellite loci were distinguishable and polymorphic with 6 types of allele-combinations. Null alleles were detected in 46.7% of loci, accounting for 11.7% of the total alleles. Four loci did not segregate in Mendelian Ratios. Three linkage groups were identified among 7 of the 15 segregating loci. Fluorescence-based automated capillary electrophoresis (ABI 310 Genetic Analyzer) that used to detect the microsatellite loci, has been proved a fast, precise, and reliable method in microsatellite genotyping.     

Efficient expression of green fluorescent protein （GFP） mediated by a chimeric promoter in Chlamydomonas reinhardtii

To improve the expression efficiency of exogenous genes in Chlamydomonas reinhardtii,a high efficient expression vector was constructed.Green fluorescent protein(GFP) was expressed in C.reinhardtii under the control of promoters:RBCS2 and HSP70A-RBCS2.Efficiency of transformation and expression were compared between two transgenic algae:RBCS2 mediated strain Tran-Ⅰ and HSP70A-RBCS2 mediated strain Tran-Ⅱ.Results show that HSP70A-RBCS2 could improve greatly the transformation efficiency by approximately eightfold of RBCS2,and the expression efficiency of GFP in Tran-Ⅱ was at least double of that in Tran-Ⅰ.In addition,a threefold increase of GFP in Tran-Ⅱwas induced by heat shock at 40°C.All of the results demonstrated that HSP70A-RBCS2 was more efficient than RBCS2 in expressing exogenous gene in C.reinhardtii.     

Evaluation of estimation methods for meiofaunal biomass from a meiofaunal survey in Bohai Bay

Studies in the coastal area of Bohai Bay,China,from July 2006 to October 2007,suggest that the method of meiofaunal biomass estimation affected the meiofaunal analysis.Conventional estimation methods that use a unique mean individual weight value for nematodes to calculate total biomass may cause deviation of the results.A modified estimation method,named the Subsection Count Method (SCM),was also used to calculate meiofaunal biomass.This entails only a slight increase in workload but generates results of g...     

南海西部浮游有孔虫含量与水深关系定量研究

南海西部4°～18°N,1085～115°E海域表层沉积物中有孔虫定量分析表明,从陆架至深海盆区,随着水深增加,底栖有孔虫丰度总体呈下降趋势;而浮游有孔虫在上陆坡区水深200～2 000 m处最丰富,向浅水和深水方向,其丰度均下降,浮游有孔虫百分含量（P）与水深（D）有明显的相关关系。但在陆架区和陆坡－深海盆区,两者关系完全不同：在陆架区随水深增加浮游有孔虫百分含量明显增大,而在陆坡－深海盆区,两者呈负相关关系。经定量拟合水深小于200 m的陆架区,浮游有孔虫含量与水深满足关系式：lnD＝0.021P＋3.208;而在水深大于200 m的陆坡－深海盆区,两者满足D＝－5263P＋52 105.2。这主要是由于陆架区随水深增加,浮游有孔虫增加,但水深大于200 m后,碳酸盐的溶解起主要作用,浮游有孔虫比底栖有孔虫更易于溶解,造成其含量随水深增加而下降。