Influence of artificial groundwater lakes on the abundance and activity of bacteria in adjacent subsurface systems

Bacterial abundances and activity, estimated by 4′,6-diamidino-2-phenylindole staining (DAPI) and the reduction of 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl tetrazolium chloride (INT), were investigated in two oligotrophic artificial groundwater lakes and the surrounding aquifers. To evaluate the effect of lake water on groundwater downstream, samples were taken from wells at different distances from the lakes, and the total number of bacteria and the number of active bacteria in these samples were compared with samples collected upstream. In addition, sterilized sandy sediments were exposed in groundwater wells to measure the number and activity of bacteria attached to particles. At one of the study sites, where the lake sediments were disturbed by dredging, total bacterial abundance and the number of respiring bacteria in the groundwater aquifer was clearly influenced by the lake water. The average bacterial abundances decreased from 2.6 ± 1.9 × 10⁵ cells ml⁻¹ in the well closest to the lake (S2) to 2.9 ± 3.8 × 10⁴ cells ml⁻¹ in the most distant one (S4), which was equivalent to cell numbers in the upstream well. The number of respiring bacteria showed a similar tendency with 1.3 ± 2.7 × 10⁴ active cells ml⁻¹ in S2 and 1.9 ± 1.5 × 10³ active cells ml⁻¹ in S4. At the second study site, which was not influenced by dredging, bacteria in the downstream wells seemed not to be affected by the lake water. The number and activity of bacteria, which colonized exposed sediments, were not significantly different in the upstream and downstream wells, indicating a minor influence of lake water on this habitat. Our results suggest that gravel-pit lakes may influence the free living bacterial assemblages in nearshore groundwater systems, but do not visibly affect numbers and activity of bacteria attached to the surface of aquifer sediments.     

Oxidative stress in the mussel Mytella guyanensis from polluted mangroves on Santa Catarina Island,Brazil

Digestive glands of the mangrove mussel Mytella guyanensis, collected at one non-polluted site (site 1) and two polluted sites (sites 2 and 3), were analysed for different antioxidant defenses, lipid peroxidation and DNA damage. Thiobarbituric acid-reactive substance (TBARS) and 8-oxo-7,8-dihydro-2'-deoxyguanosine levels were enhanced at the polluted sites. With the exception of superoxide dismutase, the activities of catalase and glutathione peroxidase were also higher at the polluted sites. Greater increases were observed in glutathione reductase, glutathione S-transferase and etoxyresorufine-O-deethylase activities at the polluted sites. Conversely, reduced glutathione content was higher at the control site. Trace metal contents in mussels collected at polluted sites were increased compared to the control site, and there were strong positive correlations between TBARS and Cu and Pb contents. M. guyanensis is routinely exposed to an oxidative stress condition at both polluted sites, and considering xenobiotic bioaccumulation in bivalve molluscs, the mangrove mussel represents an excellent bioindicator for environmental monitoring studies.     

Influence of water temperature and salinity on seasonal occurrences of Vibrio cholerae and enteric bacteria in oyster-producing areas of Veracruz, México

The influence of temperature and salinity on the occurrence of Vibrio cholerae, Escherichia coli and Salmonella spp. associated with water and oyster samples was investigated in two lagoons on the Atlantic Coast of Veracruz, Mexico over a 1-year period. The results indicated that seasonal salinity variability and warm temperatures, as well as nutrient influx, may influence the occurrence of V. cholera. non-O1 and O1. The conditions found in the Alvarado (31.12 °C, 6.27‰, pH = 8.74) and La Mancha lagoons (31.38 °C, 24.18‰, pH = 9.15) during the rainy season 2002 favored the occurrence of V. cholera O1 Inaba enterotoxin positive traced in oysters. Vibrio alginolyticus was detected in Alvarado lagoon water samples during the winter season. E. coli and Salmonella spp. were isolated from water samples from the La Mancha (90–96.7% and 86.7–96.7%) and Alvarado (88.6–97.1% and 88.6–100%) lagoons. Occurrence of bacteria may be due to effluents from urban, agricultural and industrial areas.     

Use of Plant Bioassays for the Detection of Genotoxins in the Aquatic Environment

The present article describes the use of plant bioassays for the detection of genotoxins in the aquatic environment and gives an overview of test methods, their detection spectrum for environmental mutagens and carcinogens and their limitations and pitfalls. The most widely used test systems are micronucleus assays with meiotic cells of Tradescantia and with meristematic root tip cells of Allium and Vicia. In the last years, protocols for single cell gel electrophoresis assays have been developed, which can be conducted with a variety of species. Also various gene mutation test procedures have been developed with plants but they have hardly ever been used in environmental studies. Plants detect a broad variety of environmentally relevant genotoxins, in particular directly acting compounds. Many pesticides and industrial chemicals caused positive results; plant assays are also a unique tool for the detection of DNA‐reactive carcinogenic heavy metals in the environment. In many studies with complex mixtures, positive results were obtained which indicates that plants are sufficiently sensitive to detect effects without concentration of water samples. One of the shortcomings of the use of plants as indicators is their lack of sensitivity towards certain classes of promutagens such as nitrosamines, heterocyclic amines, and polycyclic aromatic hydrocarbons (PAHs). However, the former compounds are hardly encountered in the environment and PAHs can easily be detected chemically and in other mutagenicity tests. Taken together, the currently available data show that plant bioassays are a useful component of test batteries for environmental monitoring.     

Eustatic and climatic controls on the development of the Wahiba Sand Sea, Sultanate of Oman

Aeolian sand sea accumulations can serve as valuable archives of climate change in continental environments. The Wahiba Sand Sea is situated at the northern margin of the area presently affected by Indian Summer Monsoon Circulation and it records environmental changes associated with this major climatic boundary over the last 160 000 years. The internal stratigraphy and evolution of the sand sea is investigated using a combination of outcrop, borehole, seismic and luminescence data. Proximity to the Indian Ocean means that the sand sea succession shows the influence of sea level changes on the sedimentary architecture and composition of the dune deposits. During the last two glacial periods, low global sea level was associated with a high input of bioclastic grains, reflecting the significance of subaerially exposed shelf areas as one of the main sources of aeolian sediment. The onset of aeolian sediment transport and deposition was related to the breakdown of stabilizing vegetation during arid periods that equate with sea level lowstands. The preservation of aeolian sediments by the formation of supersurfaces and associated palaeosoils took place during times of increased wetness and elevated groundwater tables. This interplay of constructive and destructive periods greatly influenced the sedimentary architecture. Oscillations of wet and dry periods between 160 000 and 130 000 years and 120 000–105 000 years ago are attributed to the evolution of a wet aeolian system. Younger periods of aeolian deposition around and after the last glacial maximum were characterized by dry aeolian conditions. No soil horizons developed during these times.     

Terrestrial laser scanning survey in support of unstable slopes analysis: the case of Vulcano Island (Italy)

Natural Hazards - The capability to measure at distance dense cloud of 3D point has improved the relevance of geomatic techniques to support risk assessment analysis related to slope instability....     

Paleomagnetism and magnetic mineralogy of metabasites and granulites from Orlica-Śnieżnik Dome (Central Sudetes)

The results of palaeomagnetic, rock magnetic, and microscopic study of Early Paleozoic metabasites and granulites from the Orlica?nie?nik Dome (OSD, Sudetes) have been combined with geochronological data. In the eastern part of the OSD (?nie?nik Massif, SM) ferrimagnetic pyrrhotite is prevalent, accompanied by various amounts of Fe-oxides. In the western part of the OSD (Orlica-Bystrzyca Massif, OBM) Fe-oxides dominate. All magnetic minerals originated during hydrothermal and weathering processes. The palaeomagnetic study revealed the presence of three secondary components of natural remanence: Late Carboniferous, Late Permian, and Mesozoic. Two Paleozoic components are related to volcanic activity in the Sudetes. They are carried by pyrrhotite and Fe-oxides and were isolated only in SM rocks. The Mesozoic component was determined in both parts of the OSD and is carried by Fe-oxides. It covers a time span, from ～160 to ～40 Ma, corresponding to a long period of alteration.     

The sanitary state of Pomeranian Bay and Gulf of Gdańsk waters during the flood of 1997

Summary Due to the 1997 summer flood, the Pomeranian and Gdańsk Bays were polluted with a significant amount of bacteriologically contaminated Oder and Vistula rivers waters. The purpose of this study was to determine the sanitary state of both bays during the flood and directly after it, the range of direction of distribution of the bacteriological contamination brought in by Vistula and Oder waters. On the basis of examination results, the sanitary state of the bays waters was found to be worse, particularly with regard to indicators: total bacterial number (at 20 °C and 37 °C) and Most Probable Number (MPN) of coliform and faecal coliform bacteria. Bacteria of the Pseudomonas group(Pseudomonas aeruginosa, Pseudomonas fluorescens), Enterobacteriaceae group (m.in.Proteus vulgaris), as well as enterococci(Streptoccus) and staphylococci(Staphylococcus) were found in water samples from Pomeranian and Gdańsk Bays. In the Pomeranian Bay the waters spread to the west (due to weather conditions), whereas in Gdańsk Bay they remained mainly in the coastal zone and then moved to the east. An improvement in the sanitary state of both bay waters was observed at the end of August. Zusammenfassung W?hrend des Sommer-Hochwassers 1997 wurden die Gew?sser der Pommerschen und der Danziger Bucht mit kontaminiertem Wasser aus den Flüssen Oder und Weichsel verunreinigt. Ziel dieser Arbeit ist es, den Grad der bakteriellen Verunreinigungen durch einstr?mendes Flu?wasser aus Oder und Weichsel in die Pommersche und die Danziger Bucht w?hrend des Hochwassers und direkt danach zu bestimmen. Auf der Basis von Messungen wurde eine Vermehrung von Colibakterien im Wasser beider Buchten unter Berücksichtigung folgender Indikatoren festgestellt: 1. Gesamtzahl an Bakterien (Wassertemperatur 20 °C and 37 °C) und 2. vermuteter Anteil von gesamtcoliformen und f?kalcoliformen Bakterien. Die Wasserproben aus der Danziger und der Pommerschen Bucht enthielten sowohl F?ulnisbakterien(Pseudomonas aeruginosa, Pseudomonas fluorescens) als auch Bakterien aus der Gruppe der Enterobacteriaceae(Proteus vulgaris), Staphylokokken(Staphylococcus) und F?kalstreptokokken(Streptococcus). Infolge Wetterver?nderung verlagerte sich das kontaminierte Wasser aus der Pommerschen Bucht nach Westen. In der Danziger Bucht hingegen blieb das kontaminierte Wasser zun?chst im Küstenbereich und verlagerte sich dann ostw?rts. Ende August wurde eine Verbesserung der Wasserqualit?t in beiden Buchten ermittelt.