Integrative taxonomic description of Plakina kanaky,a new polychromatic sponge species from New Caledonia (Porifera: Homoscleromorpha)

Four similar sponges of different colors, all unknown to science, were collected in submarine caves of New Caledonia. We aimed at determining whether the four chromotypes represented different species or phenotypic variations of a unique new species. We used an integrative taxonomic approach combining morphologic, molecular and metabolomic analyses. The main traits that define these specimens are a skeleton made of monolophose, trilophose and tetralophose calthrops only, high chemical diversity and a high abundance and diversity of prokaryotic symbionts. The symbiotic community includes two unique prokaryote morphotypes, which are described for the first time in Homoscleromorpha, and appeared to be vertically transmitted. Although several features slightly differ among chromotypes, the most parsimonious conclusion was to propose a single new species Plakina kanaky sp. nov. Our phylogenetic analysis indicated the paraphyly of the Plakina genus, with P. kanaky sp. nov. belonging to a clade that includes Plakina jani and Plakina trilopha. The present work demonstrates that integrative taxonomy should be used in order to revise the entire Plakinidae family and especially the non‐monophyletic genus Plakina.     

Molecular and distribution data on the poorly known,elusive, cave mysid Harmelinella mariannae (Crustacea: Mysida)

Mediterranean underwater marine caves harbour abundant populations of several species of mysids that are increasingly used as biological models in ecological and evolutionary studies. One exception is the species Harmelinella mariannae, described in 1989 and then hardly ever again reported in the literature. We here provide the first data on the distribution of this poorly known taxon that, contrary to expectations for a rare brooding cave dweller, we now report from Madeira Island in the nearby Atlantic, to the easternmost parts of the Mediterranean. Brief behavioural observations are added, particularly its atypical solitary habits and its feeding behaviour as a high trophic level carnivore. Molecular characterization of the different specimens captured provided three sorts of information. Mitochondrial COI and 16S haplotypes suggest different colonization waves in the Mediterranean, with one group in the Eastern Basin, two in the Marseille region in the NW part of this sea, and another group with a very wide extension from Madeira to Liguria and Malta. Mitochondrial data also support that one of the groups in Marseille might have diverged as a cryptic species of Harmelinella. 18S rRNA gene displays a single common sequence to all specimens from the four groups, and seems to confirm the original proposed placement of this taxon within the subfamily Heteromysinae, not Leptomysinae.     

Temporal and spatial zonation of macroepiphytes on Posidonia oceanica (L.) Delile leaves in a meadow off Tunisia

The aim of this survey was to study the cover and the composition of macroepiphytic species on the leaves of Posidonia oceanica in the east of Tunisia. Surveys were conducted in December 2009 (winter period), March (spring period) and August 2010 (summer period) in a fringing reef located in Chebba. At each sampling date, 15 adult leaves were randomly collected and divided into basal and apical parts. The inner face of each part was examined with ocular glasses and microscope to estimate species cover by orthogonal projection. PERMANOVA and ANOSIM were used to test for differences of cover between sampling dates and leaf parts. SIMPER, cluster analysis, and PCA were used to ordinate species assemblages. Comparison of epiphytic cover along leaf blades showed significant differences for all groups, except cyanophycea, with high cover of hydrozoans and bryozoans in the basal part and high cover of algae in the apical part. The species composition and cover also vary with sampling date; minimum values were detected in December and the epiphytic community was composed of a few pioneer species, whereas maximum epiphytic cover values were registered in August, with the epiphytic community being composed of a more mature and more diverse community, termed ‘climax’. The main regulatory factors for this distribution are discussed.     

Protracted oogenesis and annual reproductive periodicity in the deep‐sea pennatulacean Halipteris finmarchica (Anthozoa,Octocorallia)

Halipteris finmarchica is one of the most common species of deep‐sea pennatulacean corals in the Northwest Atlantic; it was recently determined to act as a biogenic substrate for other species and as a nursery for fish larvae. Its reproductive cycle was investigated in colonies sampled in 2006 and 2007 along the continental slope of Newfoundland and Labrador (Canada). Halipteris finmarchica exhibits large oocytes (maximum diameter of 1000 μm), which are consistent with lecithotrophic larval development. Female potential fecundity based on mature oocytes just before spawning was ~6 oocytes · polyp^?1 (500–6300 oocytes · colony^?1); male potential fecundity was 16 spermatocysts · polyp^?1 (5500–57,400 spermatocysts · colony^?1). Based on statistical analysis of size‐probability frequency distributions, males harboured one cohort of spermatocysts that matured inside 8–11 months, whereas females harboured two distinct cohorts of oocytes; a persistent pool of small ones (≤400 μm) and a small number (~20%) of larger ones that grew from ~400 to >800 μm over a year. Despite this difference in the tempo of oogenesis and spermatogenesis, a synchronic annual spawning was detected. A latitudinal shift in the spawning period occurred from south (April in the Laurentian Channel) to north (May in Grand Banks and July–August in Labrador/Lower Arctic), following the development of the phytoplankton bloom (i.e. sinking of phytodetritus). Prolonged oogenesis with the simultaneous presence of different oocyte classes in a given polyp is likely not uncommon in deep‐sea octocorals and could hamper the detection of annual/seasonal reproduction when sample sizes are low and/or time series discontinued or brief.     

Mortality by cause of death in Colombia: a local analysis using spatial econometrics

Journal of Geographical Systems - Colombia is undergoing major changes in mortality patterns. National- and department-level cause-specific analyses have previously been carried out, but very...