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This study aimed to optimize the purification of recombinant growth hormone from Paralichthys olivaceus. Recombinant flounder growth hormone (r-fGH) was expressed by Escherichia coli in form of inclusion body or as soluble protein under different inducing conditions. The inclusion body was renatured using two recovery methods, i.e., dilution and dialysis. Thereafter, the refolded protein was purified by Glutathione Sepharase 4B affinity chromatography and r-fGH was obtained by cleavage of thrombin. For soluble products, r-fGH was directly purified from the lysates by Glutathione Sepharase 4B affinity chromatography. ELISA-receptor assay demonstrated that despite its low receptor binding activity, the r-fGH purified from refolded inclusion body had a higher yield (2.605 mgL-1) than that from soluble protein (1.964 mgL-1). Of the tested recovery methods, addition of renaturing buffer (pH 8.5) into denatured inclusion body yielded the best recovery rate (17.9%). This work provided an optimized purification method for high recovery of r-fGH, thus contributing to the application of r-fGH to aquaculture. 相似文献
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广州城市空间形态特征与时空演化分析 总被引:3,自引:0,他引:3
城市空间形态的形成和变化是城市内、外各种社会力量相互作用的物质空间反映。本文基于历史资料和遥感影像,探讨了近百年来广州城市空间形态特征和演变过程。广州城市空间形态演变依次经历了轮形团块期、触角期、分散组团式、轴向发展期、带形发展期、新的触角期等几个阶段。利用遥感方法对1979~2004年间广州市建成区变化的监测表明,该期间广州先后经历了20世纪80年代的相对稳定期、90年代前期的快速发展期、90年代中后期的平稳发展期和近年来的高速发展期,目前是扩展速度较快的时期。 相似文献
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JI Zilu LI Ronghua WANG Chunlin ZHANG Zhouyi ZHANG Weijia MU Changkao SONG Weiwei 《中国海洋大学学报(英文版)》2022,21(1):179-185
This study investigated the effects of long-term low-salinity on the growth performance,proximate composition,amino acid and fatty acid profiles of Scylla paramamosain.The salinity of the low-salinity-culture group(LC)and maintained-salinity-cul-ture group(MC)were set at 5 and 25 respectively.After rearing for 30 d,the survival rate(SR)of the LC group was significantly lower,whereas the average daily molting frequency(ADMF)was significantly higher than those in MC group(P<0.05).There were no significant difference for weight gain rate(WGR)and specific growth rate(SGR)between the two groups.The moisture content of the LC group was slightly higher than the MC group without significant difference.The contents of ash,crude lipid and crude pro-tein in the LC group were lower than those in the MC group,but no significant differences were found except for ash content.A total of 17 free amino acids and 23 fatty acids were identified from the muscle of S.paramamosain.The total amino acids(TAA),essen-tial amino acids(EAA)and essential amino acid index(EAAI)had no significant difference between the two groups.The crabs from LC group had a significantly higher HUFA,including EPA,DHA,ARA.The percentage of n-3 PUFA and the ratio of n-3/n-6 PUFA were significantly higher in the LC group.These results indicated that S.paramamosain rearing at low salinity had significantly de-creased SR;however,the low-salinity environment significantly promoted the accumulation of unsaturated fatty acids(UFAs),espe-cially the n-3 PUFA,which might be related to the osmoregulation mechanism. 相似文献
46.
Laboratory experiments were conducted to investigate the evolution of interfacial internal solitary waves(ISWs) incident on a triangular barrier. ISWs with different amplitudes were generated by gravitational collapse. The ISW energy dissipation and turbulence processes were calculated as waves passed over the triangular barrier. Experimental results showed that ISWs were reflecting back off the triangular barrier, and shoaling ISWs led to wave breaking and mixing when waves propagated over the obstacle. Wave instability created the dissipation of energy as it was transmitted from waves to turbulence. The rate of ISW energy dissipation, the maximum turbulent dissipation, and the buoyancy diffusivity linearly increased with the increase in the incident wave energy. 相似文献
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Glacial tills are highly compressible soils composed of loosely cemented sandy silt particles. Their role, for instance, as initial filler materials in geo-technical projects along temperate glacier regions warrant a systematic evaluation of their compressive characteristics. As such, we carry out a series of onedimensional compression tests on re-compacted glacial till samples obtained from Tianmo Gully, Tibet, with the aims of evaluating their compressive behavior under controlled initial stress conditions. The yield stress was determined from the void ratio versus consolidation pressure(e-log) planes. Its compression and swelling behaviors were observed during the primary and secondary compressions of the consolidation tests. From the compression curves, a correlation can be found between the consolidation stress and the void index. In addition, we find that the compression curves of glacial tills collapse into a single curve when normalized by the compression index. The experimental results in this study provide a basic understanding of the compression characteristics of the glacial tills in Tibet, China. 相似文献
48.
山东南部沿海冬季小型底栖生物的初步研究 总被引:1,自引:0,他引:1
利用统计法、经验模型和多元聚类分析法和数学分析软件对2006年12月908项目冬季航次山东南部海域(36°15′—36°45′N,120°45′—122°00′E)小型底栖生物调查所获得的数据进行分析,获得该调查海域冬季小型底栖生物的平均丰度为(657.43±214.60)ind/10cm2,平均生物量为(847.15±427.63)μgdwt/10cm2,平均生产量为(7624.32±3848.64)μgdwt/(10cm2·a-1);还对N/C、Simpson优势度指数、Margalef多样性指数、Pielou均匀度指数和香农-威纳指数进行了计算。与2006年8月908项目夏季航次同一海域调查数据进行了比较,得到小型底栖生物的平均丰度及其优势类群线虫丰度冬季明显低于夏季,而生物量和类群多样性与夏季相当。同时,依据丰度指标对山东南部研究海域进行了初步的生态学划分,呈现出较明显的地域性。 相似文献
49.
Based on the z-coordinate ocean model HAMSOM,we introduced the internal-tide viscosity term and applied the model to numerically investigate the M2 internal tide generation and propagation in the Luzon Strait (LS).The results show that (1) in the upper 250 m depth,at the thermocline,the maximum amplitude of the generated internal tides in the LS can reach 40 m;(2) the major internal tides are generated to the northwest of Itbayat Island,the southwest of Batan Island and the northwest of the Babuyan Islands;(3) during the propagation the baroclinic energy scattering and reflection is obvious,which exists under the effect of the specific topography in the South China Sea (SCS);(4) the westward-propagating internal tides are divided into two branches entering the SCS.While passing through 118 E,the major branch is divided into two branches again.The strongest internal tides in the LS are generated to the northwest of Itbayat Island and propagate northeastward to the Pacific.However,to the east of 122 E,most of the internal tides propagate southeastward to the Pacific as a beam. 相似文献
50.
新疆荒漠地区植被覆盖度遥感估算模型十分缺乏,给荒漠化监测等相关工作带来很大不便,开展植被覆盖度遥感估算经验模型研究,对于促进和完善相关地区的生态监测及研究工作具有积极的现实意义。通过对阜康市北部沙漠南缘和克拉玛依市中部平原荒漠进行无人机航拍,利用无人机遥感提取(光合)植被信息,并将无人机航拍影像的植被覆盖度统计单元与高分辨率卫星影像像元在空间上直接相对应,获取在高分辨率卫星影像像元尺度上的植被盖度,然后通过植被覆盖度和空间上与其相对应的源自高分辨率卫星影像的NDVI数据的拟合关系,建立基于源自高分二号影像的NDVI的阜康北部沙漠植被覆盖度遥感估算线性模型以及基于源自ZY1-02C影像的NDVI的克拉玛依平原荒漠植被覆盖度遥感估算二次多项式模型。研究中所采用的无人机遥感与卫星遥感相结合、植被覆盖度统计单元与卫星像元在空间上直接对应的方法,可避免以往相关工作中常以点位测量数据代表卫星像元数据所带来的不确定性。由于所用卫星影像的NDVI数据稳定性相对不足等原因,所建立的遥感估算模型的估算精度尚相对偏低,有待于今后进一步的工作加以改进。 相似文献