Effect of copper on the growth of shrimps Litopenaeus vannamei: water parameters and copper budget in a recirculating system

Shrimps( Litopenaeus vannamei) were intensively cultured in a recirculating aquaculture system for 98 days to investigate effects of 0.3 mg/L Cu on its performance, Cu budget, and Cu distribution. Shrimps in Cu-treated systems had greater mean final weight(11.10 vs 10.50 g), body length(107.23 vs 106.42 mm), survival rate(67.80% vs 66.40%), and yield(6.42 vs 5.99 kg/m 3), and lower feed conversion ratio(1.20 vs 1.29) than those in control systems but the differences were not significant. Vibrio numbers remained stable(10 4 –10 6 colony forming units/mL) in the rearing tanks of both control and treated systems. Total ammonium-N, nitrite-N, nitrate-N, pH, chemical oxygen demand, 5-day carbonaceous biochemical oxygen demand, and total suspended solids were similar in controls and treatments. Dissolved Cu concentration in the treated systems decreased from 0.284 to 0.089 mg/L while in the control systems it increased from 0.006 2 to 0.018 mg/L. The main sources of Cu in the treated systems were the artificially added component(75.7% of total input), shrimp feed(21.0%), water(2.06%), and shrimp biomass(1.22%). The major outputs of Cu occurred via the mechanical filter(41.7%), water renewal(15.6%), and draining of the sediment trap(15.1%). The foam fractionator removed only 0.69% of total Cu input. Harvested shrimp biomass accounted for 11.68% of Cu input. The Cu concentration of shrimps in the Cu-treated systems(30.70 mg/kg wet weight) was significantly higher than that in control systems(22.02 mg/kg). Both were below the maximum permissible concentration(50 mg/kg) for Cu in seafood for human consumption in China. Therefore, recirculating systems can be used for commercial on-growing of Litopenaeus vannamei without loss of shrimp quality, even in water polluted by 0.30 mg/L Cu. The mechanical filter is the main route for Cu removal.     

Analysis of fatty acid composition of sea cucumber Apostichopus japonicus using multivariate statistics

Fatty acids （FAs） provide energy and also can be used to trace trophic relationships among organisms. Sea cucumber Apostichopus japonicus goes into a state of aestivation during warm summer months. We examined fatty acid profiles in aestivated and non-aestivated A. japonicus using multivariate analyses （PERMANOVA, MDS, ANOSIM, and SIMPER）. The results indicate that the fatty acid profiles of aestivated and non-aestivated sea cucumbers differed significantly. The FAs that were produced by bacteria and brown kelp contributed the most to the differences in the fatty acid composition of aestivated and non- aestivated sea cucumbers. Aestivated sea cucumbers may synthesize FAs from heterotrophic bacteria during early aestivation, and long chain FAs such as eicosapentaenoic （EPA） and docosahexaenoic acid （DHA） that produced from intestinal degradation, are digested during deep aestivation. Specific changes in the fatty acid composition of A. japonicus during aestivation needs more detailed study in the future.     

条斑紫菜类囊体膜蛋白质组双向电泳研究方法

以蓝绿温和胶电泳为工具,首次在国内用于条斑紫菜(Porphyra yezoensis)类囊体膜色素蛋白质复合物的研究。结果显示:(1)利用非离子型去污剂十二烷基麦芽糖苷(DM)增溶条斑紫菜类囊体膜,DM/Chla(w/w)15︰1产生的效果较好,在BN-PAGE胶中可以分离到较多较清晰的条带。(2)选取蔗糖密度层50%条带制备得到的类囊体膜样品进行第一向BN-PAGE和第二向SDS-Urea-PAGE电泳实验。第一向电泳分离出4个蛋白复合物,对第二向电泳胶上的15个蛋白点切取做质谱鉴定,检测到PSⅡ47ku,PSⅡ44ku,cytochrome f,PSⅡD2,PSⅡD1等蛋白。本实验证实了温和胶电泳与SDS电泳结合对于条斑紫菜这种原始红藻的类囊体膜研究方面应用的可行性。     

荣成天鹅湖越冬大天鹅粪便的重金属水平

为对大天鹅(Cygnus cygnus)保护及湖区管理提供基础数据,作者于2011年11月~2012年3月研究了山东荣成天鹅湖越冬大天鹅食物来源及其粪便的重金属水平。结果表明:所选择的食物来源中,Zn、As和Cr在硬毛藻(Chaetomorpha linum)中含量最高,Cd在大叶藻(Zostera marina L.)叶片中含量最高,Pb在大叶藻根茎中含量最高,Cu在石莼(Ulva pertusa)中含量最高,Hg在小麦(Triticum aestivum)中含量最高;水生植物来源的粪便中Cd含量极显著高于陆地植物来源的粪便,陆地植物来源的粪便中Cr,Pb和Hg含量显著高于水生植物来源的粪便;摄食小麦苗可能造成大天鹅对Cd和Hg的积累。     

陕西地区垂直形变分析

利用中国陕西地区三期国家一等水准网的平差成果、两期二等水准网成果、两期陕西省高程控制网成果、两期全球导航卫星系统（global navigation satellite system, GNSS）大地控制网成果、陆态网络等工程连续运行基准站及流动站成果、地理国情监测成果,分析陕西地区最新垂直运动趋势。结果表明:GNSS垂直运动与水准垂直运动趋势整体一致;20世纪70年代至2018年,关中平原垂直运动呈下降趋势,关中平原周边山区及高原垂直运动呈上升趋势;陕北地区地壳垂直运动总体上呈现上升趋势,平均上升速度约为4 mm/a;关中平原总体呈下降趋势,下降最大速度为6 mm/a;陕南地区也呈上升趋势,平均上升速度约为3 mm/a;陕北、西安局部区域的不均匀地面沉降灾害严重。     

高度约束MHE算法在伪距单点定位中的应用

针对目前全球卫星导航系统(GNSS)中伪距单点定位(SPP)技术的定位精度已不能满足现代大多数应用场景的需求,提出了一种附加高度约束的滚动时域估计(MHE)算法,以此来改善SPP的定位性能. 附加高度约束的MHE算法是将接收机的位置高度作为非线性约束加入到SPP的估计参数中,并采用近似MHE算法来进一步提高定位精度的优化算法. 结果表明:高度约束的MHE滤波比传统最小二乘(LS)的滤波具有更好的平滑特性,同时随其视窗大小的增加,其定位精度得到了进一步改善. 验证了附加高度约束MHE方案的有效性、可行性,所得结果对SPP的实际应用具有重要的参考意义.      

印度尼西亚卢瓦斯砂金矿床地质特征

卢瓦斯砂金矿位于印度尼西亚共和国苏门答腊岛东南部卢瓦斯河道及一侧阶地。矿区所处大地构造位置属喜马拉雅—马来西亚—苏门答腊火山活动带之东段。依据该砂金矿床的成矿地貌特征、地质特征、重砂矿物组合,初步认定该矿属近源型冲积型砂金矿,其砂金的物质来源于萨温河上游含金地质体,特别是火山碎屑岩中的含金石英脉和硅化脉。该砂金矿聚集于河道河漫滩及一级、二级河谷阶地中的泥、砂、砾等现代沉积物中。矿体产状受河流及两侧地形、地貌控制。     

云南大关寿山水电站地质灾害危险性评估

在工程建设中,地质灾害的发生往往与工程周边复杂的地质环境条件密切相关,山地区域由于地质地形条件复杂,使得工程地质灾害发生的可能性和危险性大大增加.以云南大关寿山水电站为例,介绍了地质灾害评估工作的基本过程和特点,对一般水电站的地质灾害危险性评估工作具有一定的参考价值.     

内蒙古科右中旗地区梅勒图组安山岩年代学特征及其地质意义

对内蒙古科右中旗地区梅勒图组安山岩锆石LA--ICP--MS U--Pb年代学、锆石Hf同位素和岩石地球化学测试分析结果表明,梅勒图组安山岩形成时代约为早白垩世中期(123~125 Ma)。锆石εHf(t)=(+5.84~+9.34),一阶段Hf模式年龄为412~553 Ma。岩石地球化学分析表明,安山岩K2O含量较高,SiO2含量和Mg#值中等,富集大离子亲石元素,高场强元素Nb、Ta、Ti等明显亏损。这些特征反映出梅勒图组安山岩的源区为新形成的富集地幔。结合地球化学特征和区域大地构造背景,研究区梅勒图组安山岩的形成可能与太平洋板块俯冲作用后的陆内伸展环境有关。     

云南剑川新民铁矿地质及成因

新民铁矿位于北北西—南南东向雪龙山—桥后断裂（弥沙河断裂）东侧之云岭褶皱束三级构造单元中,赋矿层位为中三叠统上兰组下亚段泥质灰岩.地层、构造控矿,为沉积—后期改造型铁矿床.