An overview of SAX99: acoustic measurements

A high-frequency acoustic experiment was performed at a site 2 km from shore on the Florida Panhandle near Fort Walton Beach in water of 18-19 m depth. The goal of the experiment was, for high-frequency acoustic fields (mostly In the 10-300-kHz range), to quantify backscattering from the seafloor sediment, penetration into the sediment, and propagation within the sediment. In addition, spheres and other objects were used to gather data on acoustic detection of buried objects. The high-frequency acoustic interaction with the medium sand sediment was investigated at grazing angles both above and below the critical angle of about 30°. Detailed characterizations of the upper seafloor physical properties were made to aid in quantifying the acoustic interaction with the seafloor. Biological processes within the seabed and the water column were also investigated with the goal of understanding their impact on acoustic properties. This paper summarizes the topics that motivated the experiment, outlines the scope of the measurements done, and presents preliminary acoustics results     

Cell-Specific Alkaline Phosphatase Expression by Phytoplankton from Winyah Bay,South Carolina,USA

Alkaline phosphatase expression by phytoplankton from two sites in Winyah Bay, SC, USA was investigated using nutrient-addition bioassays and cell-specific enzyme-labeled fluorescence (ELF) measurements. Our aim was to determine whether expression was group- or species-specific within the phytoplankton community. Diatoms dominated the riverine site in May, the coastal site in July, and both sites in August. Phytoplankton growth was limited by nitrogen (N) availability at the coastal site in May and the riverine site in August, but phosphate limitation was not observed. Alkaline phosphatase expression ranged from ∼30% of cells enumerated to less than 1% and was significantly reduced by inorganic phosphorus (P; 10 μM P) additions. Expression was restricted to species with low abundance, and there were no shifts in community composition consistent with alkaline phosphatase expression. Lack of phosphate limitation at higher-than-Redfield N/P ratios (up to 40:1), however, points to a potentially wider role of dissolved organic phosphorus in nutrition of Winyah Bay phytoplankton than indicated by the ELF assay.     

The use of selected genotoxicity assays in green-lipped mussels (Perna viridis): a validation study in Hong Kong coastal waters

In Hong Kong, discharges of domestic and industrial wastewaters and disposal of contaminated muds into coastal waters have resulted in high levels of persistent organic contaminants in the water column, biota and bottom sediments. Although the acute toxicity of effluents has been tested by government regulators using mortality-based bioassays, the sublethal effects of many of the pollutants, including environmental carcinogens, have not been well studied. The objective of the present work was to examine the efficiency and efficacy of using: (1) DNA strand breaks (comet assay and alkaline agarose gel electrophoresis assay); (2) oxidative DNA damage (levels of 8-hydroxy-2-deoxyguanosine in cellular DNA) and (3) micronuclei (MN) formation as early warning toxicological indicators of potential environmental carcinogen exposure. Green-lipped mussels (Perna viridis) were transplanted to study sites and retrieved after 4, 8, 12, 16 and 30 days. The bioaccumulation of organic pollutants, including the polycyclic aromatic hydrocarbons (PAHs), organochlorine pesticides (OC pesticides) and polychlorinated biphenyls (PCBs) was observed, and compared with biomarker assays. Some significant dose- and time-dependent inductions of the genotoxic biomarkers, including 8-OHdG, DNA strand breaks and micronuclei were observed. In particular, several very strong (r>0.9) and significantly positive relationships were found at all sites during days 0-12. In addition, significant strong relationships between certain organic contaminants (carcinogenic PAHs and 4 common OC pesticides) and micronuclei were detected at the polluted sites, Tsim Sha Tsui and Sai Wan Ho. The results suggest that the use of micronuclei as a biomarker is potentially effective, especially at the more polluted sites. Nonetheless, further research is required to validate their effectiveness under varying field conditions, especially to elucidate responses under lower contaminant loads, interactions between the environmental and physiological factors affecting organisms in differing coastal environments, and changes in biotransformation and DNA repair enzymes in test organisms.     

Environmental Factors Associated With Natural Methane Occurrence in the Appalachian Basin

The recent boom in shale gas development in the Marcellus Shale has increased interest in the methods to distinguish between naturally occurring methane in groundwater and stray methane associated with drilling and production operations. This study evaluates the relationship between natural methane occurrence and three principal environmental factors (groundwater redox state, water type, and topography) using two pre‐drill datasets of 132 samples from western Pennsylvania, Ohio, and West Virginia and 1417 samples from northeastern Pennsylvania. Higher natural methane concentrations in residential wells are strongly associated with reducing conditions characterized by low nitrate and low sulfate ([NO₃^?] < 0.5 mg/L; [SO₄^2?] < 2.5 mg/L). However, no significant relationship exists between methane and iron [Fe(II)], which is traditionally considered an indicator of conditions that have progressed through iron reduction. As shown in previous studies, water type is significantly correlated with natural methane concentrations, where sodium (Na) ‐rich waters exhibit significantly higher (p<0.001) natural methane concentrations than calcium (Ca)‐rich waters. For water wells exhibiting Na‐rich waters and/or low nitrate and low sulfate conditions, valley locations are associated with higher methane concentrations than upland topography. Consequently, we identify three factors (“Low NO₃^? & SO₄^2?” redox condition, Na‐rich water type, and valley location), which, in combination, offer strong predictive power regarding the natural occurrence of high methane concentrations. Samples exhibiting these three factors have a median methane concentration of 10,000 µg/L. These heuristic relationships may facilitate the design of pre‐drill monitoring programs and the subsequent evaluation of post‐drill monitoring results to help distinguish between naturally occurring methane and methane originating from anthropogenic sources or migration pathways.     

N-Body Simulations of Planetesimal Evolution: Effect of Varying Impactor Mass Ratio

We present results from direct N-body simulations of collisions between gravitational aggregates of varying size as part of a study to parameterize planetesimal growth in the Solar System. We find that as the ratio of projectile to target mass departs from unity, the impact angle has less effect on the outcome. At the same time, the probability of planetesimal growth increases. Conversely, for a fixed impact energy, collisions between impactors with mass ratio near unity are more dispersive than those with impactor mass ratio far from unity. We derive an expression for the accretion probability as a function of mass ratio. For an average mass ratio of 1:5, we find an accretion probability of ∼60% over all impact parameters. We also compute the critical specific dispersal energy Q*_D as a function of projectile size. Extrapolating to a projectile size of 1 m with a 1-km target, we find Q*_D=10³−10⁴ J kg⁻¹, in agreement with several other collision models that use fundamentally different techniques. Our model assumes that the components of each gravitational aggregate are identical and indestructible over the range of sampled impact speeds. In future work we hope to incorporate a simple fracture model to extend the range of applicable speeds and we plan to implement our results in a large-scale planetesimal evolution code.     

A Simplified Kinetic Element Formulation for the Rotation of a Perturbed Mass-Asymmetric Rigid Body

Euler's equations, describing the rotation of an arbitrarily torqued mass asymmetric rigid body, are scaled using linear transformations that lead to a simplified set of first order ordinary differential equations without the explicit appearance of the principal moments of inertia. These scaled differential equations provide trivial access to an analytical solution and two constants of integration for the case of torque-free motion. Two additional representations for the third constant of integration are chosen to complete two new kinetic element sets that describe an osculating solution using the variation of parameters. The elements' physical representations are amplitudes and either angular displacement or initial time constant in the torque-free solution. These new kinetic elements lead to a considerably simplified variation of parameters solution to Euler's equations. The resulting variational equations are quite compact. To investigate error propagation behaviour of these new variational formulations in computer simulations, they are compared to the unmodified equations without kinematic coupling but under the influence of simulated gravity-gradient torques.