Supplementation of Yeast Extract to Practical Diet Improves the Growth,Anti-Oxidative Capacity and Intestinal Morphology of Shrimp Litopenaeus vannamei

Two control diets based on the commercial formula were designed to contain high(27%, D1) and low(22%, D2) levels of fish meal, respectively. Into D2, 500, 1000 and 1500 mg kg~(-1) of yeast extract were added, respectively, yielding three experimental diets(YE1 through YE3). Shrimp(initial body weight 0.30 g ± 0.02 g) were fed with the experimental diets, five tanks each diet and 30 shrimp individuals each tank, for 8 weeks, and then challenged with Vibrio parahaemolyticus. The results showed that the specific growth rate(SGR) of shrimp in D2 was significantly lower than that of shrimp in D1(P 0.05). The SGR of shrimp in YE3 was similar to that of shrimp in D1. The feed intake of shrimp was similar between D1 and D2. The feed conversion ratio and protein efficiency ratio of shrimp were similar among all diets(P 0.05). YE significantly improved the activity of glutathione S-transferase. The concentration of glutathione(GSH) and the total serum anti-oxidative capacity(T-AOC) of D1 were significantly higher than those of shrimp feeding other diets(P 0.05). The content of serum malondialdehyde of shrimp feeding YE2 and YE3 was significantly lower than that of shrimp feeding D2(P 0.05). The thickness of intestine muscular layer of shrimp feeding YE1 and YE2 was similar to that of shrimp feeding D1. The shrimp feeding YE1 showed the highest villus height of intestine among all groups. The cumulative mortality after challenging was similar among all groups(70.00%–86.67%)(P 0.05). In conclusion, 1000–1500 mg kg~(-1) of YE was suggested to be supplemented into the practical diets to improve the growth, anti-oxidative capacity and intestinal morphology of shrimp L. vannamei.     

Morphogenesis of the Euplotid Ciliate Uronychia binucleata Young, 1922(Ciliophora,Hypotrichia)

Morphogenesis of a population of the marine euplotid ciliate, Uronychia binucleata, which was found in Yellow Sea coastal waters next to a sewage outfall at a beach near Zhanqiao Pier, Qingdao, China, was investigated using protargol staining. The main pattern of morphogenesis is typical for the genus and can be summarized as follows: 1) the oral primordium in both proter and opisthe develops de novo in a subcortical pouch. In each daughter cell, the developing adoral zone of membranelles divides into two parts. The new membranelles formed in the proter's oral primordium will replace the leftmost five parental ones; six parental membranelles are retained by the proter; 2) the undulating membranes anlage is formed and develops independently from the oral primordium within the same subcortical pouch; 3) five primary front oventral-transverse cirral anlagen appear de novo on the cell surface; 4) the marginal cirral anlagen are formed de novo; 5) the leftmost frontal cirrus develops de novo on the cell surface; 6) two caudal cirri are formed at the posterior end of the rightmost anlage while the second primordium from the right gives rise to the third caudal cirrus. In contrast to its congeners, the anlage of the leftmost frontal cirrus is formed to the right of the undulating membranes anlage and before the formation of the latter.     

Relationship between stand characteristics and soil properties of two typical forest plantations in the mountainous area of Western Sichuan,China

There is uncertainty and limited knowledge regarding stand characteristics and soil properties of middle-aged pure forest plantations in the mountainous area of Western Sichuan. Plantation forests are almost exclusively planted as monocultures in the Pengzhou forest farm in the western mountainous area of Sichuan Province. This study aimed to assess the spatial and ecological characteristics of middle-aged Cunninghamia lanceolata(Lamb.) Hook. and Cryptomeria fortunei Hooibr. in the Pengzhou forest farm. The relationships and differences between stand growth characteristics and forest soil composition were assessed on the two forests planted simultaneously within the same area and exposed to the same environmental conditions. Cr. fortunei grew sparse, and slower than Cun. lanceolata. Principal component analysis indicated that the soil properties in the Cr. fortunei plantation were better than those in the Cun. lanceolata plantation. Redundancy analysis revealed that the differences in stand structure were affected significantly by the total nitrogen content and pH value. Thus, we propose that Cr. fortunei is planted for priority consideration in the subtropical high mountain area. Further, tending operations and application of fertilizers need to be conducted in Cun. lanceolata forests. Based on theoretical and experimental data, the present study explains why local farmers are more willing to plant Cr. fortunei, and thus provides a useful theoretical reference for the development of sustainable plantations in the future.     

Evaluation of the integrated multi-satellite retrievals for global precipitation measurement over the Tibetan Plateau

The availability of high-resolution satellite precipitation measurement products provides an opportunity to monitor precipitation over large and complex terrain and thus accurately evaluate the climatic, hydrological and ecological conditions in those regions. The Global Precipitation Measurement(GPM) mission is an important new program designed for global satellite precipitation estimation, but little information has been reported on the applicability of the GPM’s products for the Tibetan Plateau(TP). The object of this study is to evaluate the accuracy of the Integrated Multi-Satellite Retrievals for GPM(IMERG) Final Run product under different terrain and climate conditions over the TP by using 78 ground gauges from April 2014 to December 2017. The results showed the following:(1) the 3-year average daily precipitation estimation in the IMERG agrees well with the rain gauge observations(R~2=0.58, P0.01), and IMERG also has a considerable ability to detect precipitation, as indicated by a high probability of detection(78%-98%) and critical success index(65%-85%);(2) IMERG performed better at altitudes from 3000 m to 4000 m with a small relative bias(RB) of 6.4%. Precipitation change was not significantly affected by local relief;(3) the climate system of the TP was divided into four climate groups with a total of 12 climate types based on the K?ppen climate classification system, and IMERG performed well in all climate types with the exception of the arid-desert-cold climate(Bwk) type. Furthermore, although IMERG showed the potential to detect snowfall, it still exhibits deficiencies in identifying light and moderate snow. These results indicate that IMERG could provide more accurate precipitation data if its retrieval algorithm was improved for complex terrain and arid regions.     

C:N:P stoichiometry of perennial herbs' organs in the alpine steppe of the northern Tibetan Plateau

The patterns of C:N:P stoichiometry across ecosystems are important in understanding biogeochemical processes. The stoichiometry of nutrients at the leaf and root level have been reported previously, but relationships of other plant organs, such as stems and the reproductive organs, remain unclear. We collected 228 samples of leaves, roots, stems and reproductive organs from 11 common plant species at 25 sites on the Tibetan Plateau to explore the relationships of C:N:P stoichiometry both within and across plant organs. The average C concentrations in the roots, leaves, stems and reproductive organs were 427.32, 410.51, 421.11 and 416.72 mg g~(-1), respectively. The shoot tissues(leaves, stems and reproductive organs) had significantly higher N and P concentrations than the roots. The N and P concentrations had a significant positive correlation within the same organ. The nutrient concentrations(N and P) and nutrient ratios(C:N, C:P and N:P) were significantly correlated across all pairwise organ combinations. Our data suggest that alpine perennial herbs share similar evolutionary histories and have constrained patterns of covariation for C concentrations, with differential patterns for N and P stoichiometry across organs. Our data also indicate that covarying sets of nutrient traits are consistent across environments and biogeographical regions and demonstrate convergent evolution in plant nutritional characteristics in extreme alpine environments.     

祁连山中段青海云杉林土壤肥力质量评价研究

以祁连山排露沟流域青海云杉林为研究对象,研究了海拔梯度上土壤肥力因子的分布特征及变化规律,并运用主成分分析法对青海云杉林土壤肥力状况进行了评价。结果表明：（1）研究区土壤呈碱性,pH值均大于8.0;高海拔地区（3 300 m）含水量达到过饱和状态,各土层含水量均大于100%;随海拔升高,全氮含量呈增大趋势,全钾含量呈减小趋势,而全磷含量呈先减小后增大趋势;不同海拔梯度速效磷含量差异不显著（P>0.05）,海拔3 300 m处速效钾含量显著高于其他海拔段（P<0.05）。（2） 不同海拔梯度下土壤有机质、全氮、全磷、全钾、速效磷和速效钾含量都有明显的“表聚效应”,其中3 300 m处0~10 cm土层有机质含量高达325.93 g·kg^-1,是本海拔段其他土层的1.6~1.8倍,是同土层其他海拔段的1.3~2.0倍。（3） 土壤肥力因子间关系密切,土壤含水量与有机质、全氮呈极显著正相关关系,与土壤容重、pH和全钾呈极显著负相关关系,土壤养分含量之间存在不同程度的显著正相关关系。（4） 不同海拔梯度土壤肥力质量为：3 300 m>3 200 m>3 100 m>3 000 m>2 900 m。     

西藏定结地区爬坡沙丘粒度特征分析

通过对西藏定结地区典型爬坡沙丘表层沉积物的形态参数、粒度组成和粒度参数等进行分析,揭示其粒度特征。结果表明：（1）爬坡沙丘表层沉积物以细砂和中砂为主,极细砂含量较少,同时含有少量粉砂和粗砂,不含粘土成分。（2）沙丘平均粒径范围1.98~2.41 Ф,分选系数变化范围为0.45~0.75,属中等分选,偏度变化范围为0.01~0.12,呈近对称至正偏分布,峰度变化范围为0.94~1.01,呈中等尖锐峰态。（3）从样品PPSQ1~PPSQ6,随着高度和距离的增加,沙丘表层沉积物平均粒径先变细后变粗,分选不断变好。（4）爬坡沙丘表层沉积物主要来源于河漫滩。（5）与雅鲁藏布江、东昆仑山地区爬坡沙丘对比可知,定结地区的沙丘粒径较粗,分选较好,主要是物源及风力分选作用的共同结果。     

雅安市雨城区壶穴形态特征

以雅安市雨城区的严桥河和周公河流域的河床壶穴为研究对象,对128处壶穴形态规模与几何参数进行详细测量。运用定量统计方法分析壶穴的分布规模和形态特征。结果表明:周公河流域壶穴大多分布在岸边大石块上,严桥河流域壶穴主要分布于河床的岩坎上;两地壶穴平均分布密度为0.43个/m2,相对其他区域较小,且壶穴的长短轴和平均口径与深度都有相对明显的规律。在平面形态上,壶穴形态多样,周公河以近圆型壶穴最多,占52.5%,严桥河以椭圆型壶穴最多,占63.6%。在垂直剖面形态上,周公河处壶穴90%属于U型,其余为V型;严桥河处壶穴V型和U型分别占52.3%和44.3%,还有3.4%属倒Ω型;两地壶穴都以浅型为主。     

天降血栓通丸对2型糖尿病大鼠血糖、大血管病变的影响

目的：研究天降血栓通丸对2型糖尿病大鼠血糖、大血管病变的影响。方法：将90只wistar大鼠随机分为正常组15只与造模组75只,通过高糖高脂饲料+链脲佐菌素诱导建立糖尿病大鼠模型,造模成功后将其随机分为模型组,天降血栓通丸早期干预组、中期干预组、晚期干预组（简称为早期组、中期组、晚期组）,盐酸吡格列酮组（西药组）,每组各15只,分别采用相应药物干预。观察大鼠一般情况,检测空腹血糖、胰岛素、胰岛素敏感指数、细胞间黏附分子-1（ICAM-1）水平。结果：造模组与空白组比较,胰岛素、胰岛素敏感指标、体质量、第13周空腹血糖差异均有统计学意义（P＜0.01或P＜0.05）;药物干预后空腹血糖水平ICAM-1模型组、各治疗组与空白组比较,各治疗组与模型组比较,差异均有统计学意义（P＜0.05）。结论：天降血栓通丸无降糖作用,其早期干预能有效降低ICAM-1,这可能是其对2型糖尿病大鼠大血管病变影响的机制之一。     

Effects of stocking density on the growth and immunity of Atlantic salmon salmo salar reared in recirculating aquaculture system(RAS)

Atlantic salmon reared in recirculating aquaculture system(RAS) may lead to inappropriately high stocking density, because ?sh live in a limited space. Finding the suitable stocking density of Atlantic salmon reared in RAS is very important for RAS industry. In this paper, the in?uence of stocking density on growth and some stress related physiological factors were investigated to evaluate the ef fects of stocking density. The ?sh were reared for 220 days at ?ve densities(A: 24 kg/m^3; B: 21 kg/m^3; C: 15 kg/m^3; D: 9 kg/m^3 and E: 6 kg/m^3). The results show that 30 kg/m^3 might be the maximum density which RAS can af ford in China. The stocking densities under 30 kg/m^3 have no ef fect on mortality of Atlantic salmon reared in RAS. However, the speci?c growth rate(SGR), ?nal weight and weight gain in the high density group were signi?cantly lower than the lower density groups and middle density groups. Moreover, feed conversion rate(FCR) had a negative correlation with density. Plasma hormone T_3 and GH showed signi?cant decrease with the increase of the stocking density of the experiment. Furthermore, thyroid hormone(T 3), GH(growth hormone) activities were decreased with stocking density increase. However, plasma cortisol, GOT(glutamic oxalacetic transaminase) and GPT(glutamic pyruvic transaminase) activities were increase with stocking density increase. And the stocking density has no ef fects on plasma lysozyme of Atlantic salmon reared in RAS. These investigations would also help devise effcient ways to rear adult Atlantic salmon in China and may, in a way, help spread salmon mariculture in China.