Comet assay in phytoplankton as biomarker of genotoxic effects of environmental pollution

The alkaline comet assay was tested on different microalgae: the dinoflagellates, Karenia mikimotoi and Alexandrium minutum, and the diatom, Chaetoceros gracilis. The microalgae were exposed during their exponential growth to the model direct genotoxicant, hydrogen peroxide (1h, 5 and 100muM H2O2). Following H2O2 exposure, the comet assay was validated only for K. mikimotoi for which genotoxicity was observed from the lowest tested concentration of 5 microM with a concentration-dependent effect. C. gracilis was too small in size (4 microm) to be correctly analysed. For A. minutum, our lysis buffer was not strong enough to digest the cellulosic thecal plates. For K. mikimotoi, the comet assay was thus applied for the study of the genotoxic effects of different pesticides: epoxiconazole (as Opus formulation), chlorpyriphos-ethyl (as Dursban formulation) and endosulfan at 1, 10 and 100 microg of active substance/L for 24h. Exposure to epoxiconazole in formulation resulted in an increase in the extent of DNA strand breaks at the highest tested concentration icro/L. Endosulfan exposure resulted in DNA damage for K. mikimotoi nuclei. Genotoxicity was observed from 1 microg/L of endosulfan and was not concentration dependent.     

The role of water mass dynamics in controlling bacterial abundance and production in the middle Adriatic Sea

Month-to-month fluctuations in the abundance of bacteria and heterotrophic nanoflagellates (HNF) and bacterial production, as well as various chemical (nutrients, oxygen) and physical (salinity, temperature) parameters were analysed at a station located in the open middle Adriatic Sea during one decade (1997-2006). Being influenced by both coastal waters and open Adriatic circulation in the surface layer, and by the deep Adriatic water masses in the deep layers (100 m), this station is quite suitable for detecting the environmental changes occurring in the open Adriatic Sea with respect to the circulation of its water masses and their long-term changes and anomalies. Multivariate methods were used to identify seasonal and inter-annual changes of the investigated parameters, associating observed changes to the changes in Adriatic water masses and circulation regimes. The analyses showed that bacterial abundance and production were controlled by different water mass dynamics during 1997-2001 compared to 2002-2006 period, particularly noticeable in different seasonal patterns of biological parameters. The interplay between North Adriatic Dense Water (NAdDW) and Levantine Intermediate Water (LIW) resulted in a change in the available nutrients (NAdDW is poor in orthophosphates), and as a consequence different bacterial abundance and production. A few periods were examined in detail, such as 2004, when LIW inflow was particularly strong and was accompanied by an increase of bacterial and HNF abundances, as well as of bacterial production.     

Argo floats revealing bimodality of large-scale mid-depth circulation in the North Atlantic

Analysis of Argo float trajectories at 1 000 m and temperature at 950 m in the North Atlantic between November 2003 and January 2005 demonstrates the existence of two different circulation modes with fast transition between them. Each mode has a pair of cyclonic - anticyclonic gyres. The difference is the location of the cyclonic gyre. The cyclonic gyre stretches from southeast to northwest in the first mode and from the southwest to the northeast in the second mode. The observed modes strongly affect the heat and salt transport in the North Atlantic. In particular, the second mode slows down the westward transport of the warm and saline water from the Mediterranean Sea.     

阿尔沃兰海（Alboran Sea）中碳酸盐岩丘的发现：梅利利亚海山区

本文主要研究发现在阿尔沃兰海东南边缘（摩洛哥陆缘）的一群全新世海山——梅利利亚海山区（MMF）。我们通过条带测深、浅地层声学剖面、旁侧声纳、高分辨率地震调查以及沉积物取样等手段对MMF海山区进行了研究。该海山群位于陆缘下倾部分的上陆坡至陆坡中部,西北-东南走向,覆盖面积超过20km2。     

Glacier reconstruction of La Covacha Massif in Sierra de Gredos(central Spain) during the Last Glacial Maximum

A paleoglacier reconstruction during the Last Glacial Maximum(LGM) is presented for a high mountain area of La Covacha massif in Sierra de Gredos(Iberian Central System) in central Spain. The moraines that, according with the previous literature, had formed during the global LGM, were mapped through photo interpretation of digital aerial photographs and 3D images and detailed field surveys. The topography of the paleoglaciers was estimated using a simple steady-state model that assumed a perfectly plastic ice rheology, reconstructing the theoretical ice profiles and obtaining the ice thickness of the glaciers during the LGM. The reconstruction of the glaciers was carried out using automated and semi-automated physically-based models, obtaining more realistic results with semi-automated models. According to our study, the paleoglaciers in the study area covered an area of 34.79 km~2 during the global LGM, with a maximum ice thickness of 366 meters in La Vega gorge, a total volume of 34.25 × 108 m~3 and a mean paleoELA of 1932 meters. Most of the ice(～82%) was in paleoglaciers facing north, and the rest was in paleoglaciers with other orientations. This emphasizes the importance of orientation in relation to glacier dynamics and ice accumulation. The results obtained in the calculation of paleoELAs during the global LGM in Gredos are average compared to the Iberian mountains of the Northwest where the values were much lower, and with respect to those of the Southwest, much higher. This demonstrates the importance of the exposure of Mediterranean mountains to the humidity sources coming from the NW during the global LGM, as is the case at present.     

Structural Setting and Kinematic Analysis of the Halaban Region,Eastern Arabian Shield,Saudi Arabia

The vorticity analysis technique was applied to measure the different lithological units,such as schist,metagranite and metavolcano-sedimentary rocks,which are present in the Halaban region.This work aims to interpret the relationship between the different lithologies and the tectonic setting,in order to elucidate the nature of kinematic analysis in the Halaban region.The kinematic analyses were applied to feldspar porphyroclasts,quartz and hornblende for twentysix samples.The kinematic vorticity number (W_m) for deformed rocks in the study area ranged from～0.6 to 0.9.The direction of the long axes for finite strain data (X axes) revealed a WNW trend with shallow dipping.The direction of the short axes for finite strain data (Z axes) were represented by vertical with associated horizontal foliation.The results of the kinematic vorticity and strain analyses are characterized by simple shear with different degrees of deformation in the Halaban region.Furthermore,our finite strain data shows no significant volume change during deformation.The subhorizontal foliation was synchronized with thrusting and deformation.Furthermore,throughout the overlying nappes,the same attitudes of tectonic contacts are observable,the nappes in the orogens being formed from simple shear deformation.     

内蒙古鄂尔多斯高原古冰缘遗迹科学考察研究进展

冰缘遗迹(特别是冷生楔形构造及融冻褶皱)是重建古气候及第四纪晚期多年冻土环境的重要证据.内蒙古鄂尔多斯高原是我国北方地区冰缘现象最为发育的地区之一.为准确了解鄂尔多斯高原冰缘遗迹类型及其分布特征、区域冻土演化历史等,中国科学院西北生态环境资源研究院和荷兰自由大学共同组成科研小组,于2018年5—6月组织了"鄂尔多斯高原...     

Challenges in the Detection and Attribution of Northern Hemisphere Surface Temperature Trends Since 1850

Since 2007, the Intergovernmental Panel on Climate Change(IPCC) has heavily relied on the comparison between global climate model hindcasts and global surface temperature(ST) estimates for concluding that post-1950s global warming is mostly human-caused. In Connolly et al., we cautioned that this approach to the detection and attribution of climate change was highly dependent on the choice of Total Solar Irradiance(TSI) and ST data sets.We compiled 16 TSI and five ST data sets and found by alter...     

Effect of Matching Algorithm and Profile Shape on Pulsar Pulse Time of Arrival Uncertainties

For high-precision pulsar timing analysis and low-frequency gravitational wave detection, it is essential to accurately determine pulsar pulse times of arrival(ToAs) and associated uncertainties. To measure the ToAs and their uncertainties, various cross-correlation-based techniques can be employed. We develop methodologies to investigate the impact of the template-matching method, profile shape, signal-to-noise ratio of both template and observation on ToA uncertainties. These methodologies are...