日本凋毛藻碳酸酐酶基因及其编码的蛋白特征

碳酸酐酶CA(EC 4.2.1.1)是含锌原子的金属蛋白酶,该酶的基本功能是催化CO₂与HCO₃-间的相互转换[1].该反应在许多生物过程中都发挥着重要的作用,例如离子交换、呼吸作用、pH稳定性、捕获CO₂和光合作用等[2~5].CA广泛分布于各种生物中,已知的CA酶根据氨基酸序列被分为-αCA,-βCA和γ-CA三种类型[5~7].这3种类型的CA酶序列间的相似性很低,被认为是由不同的途径进化而来的[7].α-型的CA酶在动物、植物、绿藻、细菌和蓝细菌中都有发现[7],在藻类中报道的α-型CA还很少.迄今为止只在衣藻中发现2种异构体,在盐藻中发现3种[8,9],在红藻中还未见报道.     

Impact of Grazing Exclusion on the Surface Heat Balance in North Tibet

The grazing exclusion program used by the Tibetan government to protect the ecological environment has changed the vegetation and impacted the surface heat balance in North Tibet. However, little information is available to describe the in?uences of the current grazing exclusion program on local surface heat balance. This study uses the records of fenced grassland patch locations to identify the impact of grazing exclusion on surface heat balance in North Tibet. The records of fenced grassland patch locations, including the longitude, latitude, and elevation of the vertices of each fenced patch (polygon shapes), were provided by the agriculture and animal husbandry bureaus of the counties where the patches were located. ArcGIS 10.2 was used to create polygon shapes based on patch location records. Based on satellite data and the surface heat balance system determined by the model, values for changes in land surface temperature (LST), albedo and evapotranspiration (ET) induced by grazing exclusion were obtained. All of these can influence surface heat balance and alter the fluctuation of LST in the northern Tibetan Plateau. The LST trends for day and night showed an asymmetric diurnal variation, with a larger magnitude of warming in the day than cooling at night. The maximum decrease in absorbed shortwave of LST (-0.5 - -0.4 ℃ per decade) occurred in the central region, while the minimum decrease (-0.2 - -0.1 ℃ per decade) occurred in the eastern region. The decreased latent heat lead to the LST increased maximum (>1 ℃ per decade) occurred in the central region, The eastern region increased at a rate of 0.2-0.5 ℃ per decade, while the minimum increase (0-0.1 ℃ per decade) occurred in the northwestern region.     

Sensitivity evaluation of the flash flood warning system introduced to ungauged small mountainous basins in Korea

This study evaluates the uncertainty involved in the determination of the flash flood guidance(FFG) of the flash flood warning system(FFWS) for a small mountainous region(FFWS_MR)in Korea. The sensitivity is evaluated both at each step to determine the FFG and for all steps together. The results show that the relative difference of the FFG is about 50% of the current system, most of which involves the channel width. Especially, the use of some specific empirical equations to estimate the major parameters results in a considerable amount of the relative difference of the FFG. In addition, though only four basins were field-surveyed, it is found that the indirect estimation of the major parameters always introduces some amount of additional uncertainty. In conclusion, accurate estimation of the major parameters must be the most important procedure to derive an accurate FFG, among which the channel cross-section at the exit of the basin lies at the center.     

An overview of socio-economic and ecological perspectives of Fiji's inshore reef fisheries

This paper reviews the socio-economic and ecological context of Fijian reef fisheries. This review is deemed necessary because improved understanding of the state and trends of Fiji's coral reef fisheries on a national level is required for designing an effective management plan for Fiji's inshore reef fisheries. The most important point that emerges from our review is that despite numerous studies of Fiji's reef fisheries, the current status of reef-associated fisheries at the national level is still uncertain due, mainly, to the lack of dependable data on the subsistence fisheries. This in turn leads to uncertainty about how the continuation of fishing, in particular, fishing focused on target species for the coral reef resources trade, will affect fishing communities and the ecosystem.     

Multivariate analysis of fluorescence and source identification of dissolved organic matter in Jiaozhou Bay, China

Hierarchical clustering analysis and principal component analysis (PCA) methods were used to assess the similarities and dissimilarities of the entire Excitation-emission matrix spectroscopy (EEMs) data sets of samples collected from Jiaozhou Bay, China. The results demonstrate that multivariate analysis facilitates the complex data treatment and spectral sorting processes, and also enhances the probability to reveal otherwise hidden information concerning the chemical characteristics of the dissolved organic matter (DOM). The distribution of different water samples as revealed by multivariate results has been used to track the movement of DOM material in the study area, and the interpretation is supported by the results obtained from the numerical simulation model of substance tracing technique, which show that the substance discharged by Haibo River can be distributed in Jiaozhou Bay.     

虾夷扇贝天然苗种筛选装置的研究与应用

随着贝类底播增养殖产业的迅猛发展,为保证底播贝苗的成活率,养殖企业对天然苗种更加重视。天然苗种采用中间育成方法,需要对苗种进行筛选作业,但目前筛选作业仍以人工作业为主,存在作业效率低、劳动强度大等问题。作者通过对大连地区虾夷扇贝(Patinopecten yessoensis)天然苗种筛选作业调研的基础上,设计了满足生产实际需求的扇贝苗种筛选装置,可实现3种规格贝苗的筛选作业。试验分2阶段进行,第1阶段对影响筛选准确性的因素进行正交分析于验证,确定了最佳工艺参数;第2阶段在大连旅顺进行的生产性对比试验得出,机械筛选的平均准确率为91.33%,误差率低于10%,且5组筛选结果无显著性差异(P0.05);同时机械筛选的平均作业效率为80g/(人/min),约为人工筛选作业效率的8倍。由此说明扇贝苗种筛选装置具有较高的准确性、稳定性和高效性,具有推广应用价值。     

Risk identification and perception in the fisheries sector: Comparisons between the Faroes,Greece, Iceland and UK

This paper presents the findings of risk identification and risk perception research conducted in relation to the fisheries systems of four distinct and diverse European countries: Faroes, Iceland, Greece and the UK. Risk research traditionally attempts to quantify the potential threat or consequences from a range of risk events or hazards. This research, however, adopted a social sciences perspective and so assumed that a risk event or hazard can mean different things to different people and that these perceptions are also context and culturally dependent. Risk perceptions were examined and risk registers developed in each country for a range of stakeholder groups. A ‘mental modelling’ approach was adopted in a series of qualitative interviews. Findings were examined in terms of a wide range of psychological, social and cultural risk theories. Differences in risk perceptions were noted between stakeholder groups and countries and contextual influences were examined such as the widely differing fisheries management systems used in each country. This research provides one of the first attempts to systematically evaluate risks and perceptions across a range of fisheries-systems. The findings support social science theories which argue that risk is a subjective, as opposed to objective, concept and that this subjectivity will therefore affect our attempts to assess and manage those hazards we think we can potentially influence or control.     

Multivariate analysis of flood characteristics in a climate change context of the watershed of the Baskatong reservoir,Province of Québec,Canada

The analysis of the impact of climate change (CC) on flood peaks has been the subject of several studies. However, a flood is characterized not only by its peak, but also by other characteristics such as its volume and duration. Little effort has been directed towards the study of the impact of CC on these characteristics. The aim of the present study is to evaluate and compare flood characteristics in a CC context, in the watershed of the Baskatong reservoir (Province of Québec, Canada). Comparisons are based on observed flow data and simulated flow series obtained from hydrological models using meteorological data from a regional climate model for a reference period (1971–2000) and a future period (2041–2070). To this end, two hydrological models HSAMI and HYDROTEL are considered. Correlations, stationarity, change‐points, and the multivariate behaviour of flood series were studied. The results show that, at various levels, all flood characteristics could be affected by CC. Copyright © 2011 John Wiley & Sons, Ltd.