Evaluation of acetylcholinesterase activity in several zooplanktonic crustaceans

Zooplankton are an essential component of the marine and estuarine food chains. The ecotoxicological risk to zooplanktonic communities, estimated through the use of the biomarkers, can be used as an early warning signal of a potential alteration of the ecosystem health. The aim of this project was to evaluate the potential use of several biomarkers (esterases, mixed function oxidases, porphyrins) in zooplanktonic organisms. The acetylcholinesterase activity (AChE) was determined in homogenates of whole organisms of 10 different zooplanktonic crustaceans. Mean activity of AChE was 10.05 micromol/min/g for Acartia margalefi; 3.30 for Acartia latisetosa; 79.70 for Siriella clausi; 49.97 for Diamysis bahirensis; 7.48 for Siriella armata; 14.20 for Mysidopsis gibbosa; 4.49 for Euphausia crystallorophias; 1.66 for Euphausia superba; 2.74 for Streetsia challengeri; 13.26 for Meganycthiphanes norvegica. The species moreover showed a linear increase in enzyme activity in relation to the increase in sample concentration. The key result of this study concerns the different AChE activity basal values of the crustaceans' different species.     

Evaluation of toxicity in tributaries of the Mersey estuary using the isopod Asellus aquaticus (L.)

Increasing numbers of industrial, agricultural and natural chemicals are present in sewage effluent and are known to elicit toxic effects in laboratory exposures, but little is known of their combined sub-lethal effect in the field. In this study, a combination of esterase activity and ventilation rate assays was performed to determine the neurological and physiological function of the freshwater crustacean Asellus aquaticus (L.) at sites above and below a sewage treatment works (STW). Cholinesterase and carboxylesterase activities were significantly inhibited (n=8, P<0.05) and ventilation rates increased (n=8, P=0.0001) in A. aquaticus at STW sites compared to those from reference sites, indicating a decrease in neurological and physiological function. The ecological relevance of these findings for the population dynamics of the organisms in the field is discussed.     

Burial and scour around short cylinder under progressive shoaling waves

The results of a laboratory experimental program aimed at better understanding the scour around and burial of heavy cylindrical objects under oscillating flow on a sandy bed are described. This study was motivated by its application to the dynamics of isolated cobbles/mines on a sandy floor under nonlinear progressive waves, such as that occur in shallow coastal waters beyond the wave-breaking region. In the experiments, nonlinear progressive waves were generated in a long wave tank of rectangular cross-section with a bottom slope. Model mines (short cylinders) were placed on the sandy bottom and the temporal evolution of the bed profile and the velocity field in the near field of the object were observed. Experiments were conducted at relatively high Reynolds numbers for a range of flow conditions, which can be characterized by the Keulegan–Carpenter number and Shields parameter. Depending on the values of these parameters, four different scour regimes around the cylinder including periodical burial of cylinder under migrating sand ripples were observed; they were classified as: (i) no scour/burial, (ii) initial scour, (iii) expanded scour, and (iv) periodic burial cases. A scour regime diagram was developed and the demarcation criteria between different regimes were deduced. Semi-empirical formulae that permit estimation of the scour depth with time, the equilibrium maximum scour depth and length, and conditions necessary for the burial of the cylinder as a function of main external parameters are also proposed.     

Modelling of flow around a near-bed pipeline with a spoiler

Flow around a pipeline with and without a spoiler near a smooth wall is simulated by solving the Navier–Stokes equations. Finite-difference formulation with a second-order upwind scheme in a curvilinear coordinate system is employed. The influences of the spoiler on hydrodynamic forces, pressure distribution, vortex shedding frequency, velocity profile under the pipe, as well as shear stress on the wall are investigated. The attachment of a spoiler significantly increases drag, root-mean-square (RMS) lift, flow through the gap between the pipe and the wall and shear stress on the seabed around the pipe. The spoiler also generates a non-zero mean downward force on the pipeline, which may enhance the self-burial of the pipeline.     

Upstream dams and downstream clams: growth rates of bivalve mollusks unveil impact of river management on estuarine ecosystems (Colorado River Delta, Mexico)

We studied how the extensive diversion of Colorado River water, induced by dams and agricultural activities of the last 70 years, affected the growth rates of two abundant bivalve mollusk species (Chione cortezi and Chione fluctifraga) in the northern Gulf of California. Shells alive on the delta today (‘Post-dam’ shells) grow 5.8–27.9% faster than shells alive prior to the construction of dams (‘Pre-dam’ shells). This increase in annual shell production is linked to the currently sharply reduced freshwater influx to the Colorado River estuary. Before the upstream river management, lower salinity retarded growth rates in these bivalves. Intra-annual growth rates were 50% lower during spring and early summer, when river flow was at its maximum. Growth rates in Chione today are largely controlled by temperature and nutrients; prior to the construction of dams and the diversion of the Colorado River flow, seasonal changes in salinity played an important role in regulating calcification rates.Our study employs sclerochronological (growth increment analysis) and geochemical techniques to assess the impact of reduced freshwater influx on bivalve growth rates in the Colorado River estuary. A combination of both techniques provides an excellent tool to evaluate the impact of river management in areas where no pre-impact studies were made.     

Esterases as biomarkers in Nereis (Hediste) diversicolor exposed to temephos and Bacillus thuringiensis var. israelensis used for mosquito control in coastal wetlands of Morbihan (Brittany,France)

Since 1998, a biomonitoring programme has been implemented to assess the potential impact of chemical mosquito control on macroinvertebrates of the coastal wetlands of Morbihan (Brittany, France). Acetylcholinesterase and carboxylesterases were used as biomarkers to assess the effects of Abate 500e (a.i. temephos) and Vectobac 12 AS (a.i. endotoxins of Bacillus thuringiensis var. israelensis, Bti) in Nereis (Hediste) diversicolor. Esterase inhibition revealed a marked impact of temephos, suggesting preferential contamination of the worms through the food. In Bti-exposed N. diversicolor, random variations of esterase activities were observed, that could not be attributed to the larvicide. However, esterases only reflected indirect physiological effects of Bti, and further investigations are needed to identify biomarkers more specific of Bti endotoxins.     

The CANALES experiment (1996-1998). Interannual, seasonal, and mesoscale variability of the circulation in the Balearic Channels

Repeated hydrographic casts, mooring time series and satellite sea surface temperature collected during the CANALES experiment (1996–98) are used to describe the thermohaline circulation in the Balearic Channels (western Mediterranean) and to analyze its variability. Mass transports are estimated by inverse calculations. The role played by each channel in the meridional water exchange is clarified: the Ibiza Channel funnels southward cool, saline, northern waters whereas the Mallorca Channel appears as the preferred route for the northward progression of warm, fresh, southern waters. A neat interannual trend is revealed by the continuous decrease of the amount of Western Mediterranean Intermediate Waters (WIW) brought by the Northern Current, reflecting the increase in temperature of the winter mixed layer in the northern Mediterranean that occurred each year between 1996 and 1998. A clear seasonal signal was also seen in the transport of the Northern Current which decreased from 1 to 1.4 Sv in winter to < 0.5 Sv in summer. The current intensified again in fall. A number of mesoscale eddies, from 20 to 70 km in size, most of them anticyclonic vortex eddies were brought by the unstable Northern Current, these eddies strongly perturbed the water exchange in the Ibiza Channel forcing retroflections of northern waters back to the north-east into the Balearic Current. These eddies either stayed stalled for several months in the Gulf of Valencia to the north of the channel, or were slowly funnelled southward through the channel narrows. A decreasing trend was observed in the mesoscale activity of the Northern Current between 1996 and 1998. Conversely, large, anticyclonic eddies, 150-km diameter, progressively invaded the Algerian Basin to the south of the channels in 1997–98 and forcing northward inflows (up to 0.75 Sv) of fresh and warm waters of Atlantic origin (AW) into the Mallorca Channel. The marked interannual differences observed in both northern and southern eddy activity may be linked to the interannual variability of the large scale thermohaline circulation.     

A field-based population model for the sediment toxicity test organism Leptocheirus plumulosus: I. Model development

We describe the development of a stage-structured population matrix model for the estuarine amphipod Leptocheirus plumulosus, a sediment toxicity test organism, based on regular sampling of a natural population for approximately 2 years. Annual population dynamics were characterized by abundance peaks in the spring and fall, and low densities in the summer and winter. We use a sequence of seasonal projection matrices to capture these qualitative patterns in population dynamics. We compare two methods for estimating transition probabilities: multiple regression and truncated singular value decomposition (SVD). Overall, SVD gave more realistic parameter estimates than regression, although fecundity estimation was problematic. There were predictable trends in growth, survival and fecundity within years. The SVD-derived model provides a framework for assessing the population-level effects of contaminants on Leptocheirus.     

Historical record of heavy metals in a highly contaminated Mediterranean deposit: The Besòs prodelta

The Besòs prodelta, located around a highly populated area, receives the discharge of the Besòs River, one of the most contaminated rivers discharging into the Mediterranean sea. The record of heavy metals accumulated in this prodelta is studied to assess the sedimentary behaviour and the history of the anthropogenic heavy metals in the area.

Full-size image

dated sediments have allowed the identification of the sedimentation patterns and the elaboration of dated heavy metals and cenospheres profiles, which reflected the evolution of the anthropogenic impact in this marine environment. Sediments accumulated during the 18th and 19th centuries were already affected by a moderate heavy metal contamination. This contamination increased drastically in the sediments accumulated during the last decades. This data is well correlated with the increase in industrialisation, population and energy consumption in the Besòs drainage basin during the last century.