近海N_P营养盐的研究现状与进展

海洋中N、P等营养盐是海洋生态系统中的重要营养元素,是海洋生物生存和繁殖的能量来源.同时,营养盐又具有复杂多变的化学地质作用,使得近海生态环境中营养盐的研究一直是海洋研究的热点之一,对海水营养盐的研究历史几乎与海洋化学发展史同步.由于环境问题的日益突出,营养盐的研究成为目前海洋及相关科学研究项目中的核心内容之一.笔者通过回顾当今国内外近海环境营养盐调查研究的历史和综述当今国内外该学科的研究现状,概括了该学科的主要进展,并指出存在的问题和今后的发展方向.     

�㶫������������˫����¶�λ�������

???????λ????????????2004??1???2007??5??ML??1.5????????????ж?λ??????????2004??9??17?????????4.9????????????????????????????????????????ML??2.0?????????????б?????????????4.9???????????????????????????λ???????￡????????1?±?????????????????????????????????4.9?????????????????????     

Sequence analysis and quantitative detection of Norwalk-like viruses in cultured oysters of China

We isolated 4 Norwalk-like viruses (NLVs) contaminated oysters from 33 Chinese oysters collected from local commer-cial sources of Shandong Province. After amplification of the RNA-dependent RNA polymerase (RdRp) region of NLVs genomes with RT-PCR, the open reading frame 1 (ORF1) of the RdRp was sequenced and subjected to multiple-sequence alignment. The re-suits showed that NLVs in the four isolates belong to genogroup Ⅱ. The sequence comparison showed that the similarity between four Chinese oyster isolates were higher than 99.0%, which indicated that NLVs prevalent in close areas have high homogeneity in genome sequences. In addition, the most conserved sequences between diverse NLVs were used to design primers and TaqMan probes, then the real-time quantitative PCR assay was performed. According to the standard curve of GII NLVs, the original amounts (copies) of NLVs in positive patient's fecal isolate, positive Japanese oyster isolate, and the Chinese oyster isolate were 8.9×108, 1.25×108 and 4.7×101 respectively. The detecting limit of NLVs was 1×101 copies. This study will be helpful for routine diagnosis of NLVs pathogens in foods and thus for avoiding food poisoning in the future.     

西施舌(Coelomactra antipuata)群体遗传多样性与分化的研究

采用不连续垂直板聚丙烯酰胺凝胶电泳技术,对广西北海、福建漳港和江苏启东3个西施舌自然群体的7个等位酶12个位点进行检测分析.结果表明,西施舌群体的遗传多样性较高,平均每位点等位基因数目(A)范围为1.5833-2.0000、平均每位点有效等位基因数目(Ae)范围为1.2850-1.5373、多态位点百分数(P)为33.33%、观察杂合度(Ho)为0.2389-0.3083、期望杂合度(He)为0.1496-0.2041之间.西施舌群体间有一定的遗传分化,遗传分化系数(FST)为0.0719,福建漳港群体与广西北海群体之间的FST为0.0413,但福建漳港群体和广西北海群体与江苏启东群体之间的FST为0.0505.     

Least Square Finite Element Method for Viscous Splitting of Unsteady Incompressible Navier–Stokes Equations

In order to solve unsteady incompressible Navier–Stokes(N–S) equations, a new stabilized finite element method,called the viscous-splitting least square FEM, is proposed. In the model, the N–S equations are split into diffusive and convective parts in each time step. The diffusive part is discretized by the backward difference method in time and discretized by the standard Galerkin method in space. The convective part is a first-order nonlinear equation.After the linearization of the nonlinear part by Newton's method, the convective part is also discretized by the backward difference method in time and discretized by least square scheme in space. C~0-type element can be used for interpolation of the velocity and pressure in the present model. Driven cavity flow and flow past a circular cylinder are conducted to validate the present model. Numerical results agree with previous numerical results, and the model has high accuracy and can be used to simulate problems with complex geometry.     

The use of automated coal petrography in determining maceral group composition and the reflectance of vitrinite

Using photometric and image analysis methods, a reasonable technique and procedure have been developed for the determination of maceral group composition and the reflectance of vitrinite. This procedure is introduced by an optimum method for seeking the interval values of each maceral group, i.e., from the “fingerprint” reflectance histogram, it seeks reasonable interval values and distribution values for vitrinite and other maceral groups. After checking and correction, these values can be put into a program and computerized. This method had advantages such as increased speed of analysis and reproducibility of the results.     

桩基承载力自平衡试验方法在昆明地区的应用研究

概述了自平衡法的基本原理,对云南某化工有限公司5×105t/a合成氨煤气化装置中的三根试桩采用自平衡新方法进行了桩基承载力测试,对测试数据进行了整理和分析。应用结果表明,测试取得较好的工程效果。     

杨山晚古生代沉积盆地成因类型及其与桐柏-大别造山带关系的探讨

杨山晚古生代沉积盆地位于桐柏-大别山北麓,它具有明显的前陆盆地沉积特点,由早期(D₂?—C₁)的复理石建造到晚期(C₁—P?)的磨拉石建造;古生物地理分析表明其与华北、扬子陆块都有密不可分的联系,其间不可能有古洋盆的存在,因而它应当是桐柏一大别造山带碰撞造山过程中形成的前陆盆地。杨山晚古生代前陆盆地的形成说明,扬子陆块和华北陆块的陆-陆碰撞起始于晚泥盆世之前(S₃—D₂),而桐柏-大别造山带中生代的构造事件则可能代表一次大规模陆内逆冲-推覆作用。     

大洋钻探与西太平洋构造

菲律宾海的深海钻探证了弧间扩张和残留弧形模式。小笠原－马里亚纳弧前区的钻探表明，俯冲带初生时伴着海底扩张，形成了异常宽阔的火山活动带。有许多出上蛇绿岩形成于俯冲带初生时的海底扩张环境。日本海的钻探揭示了日本边缘盆地的打开和关闭，新近形成的日本海东缘板边界对华北地区的构造有重要制约作用，中国东海和南海占有独特的构造位置，布置大洋钻探意义重大。文章还强调了中国地质学家从全球地质学的观点研究问题（而不仅     

Gold concentration from well water by the hydroelectrical extraction method

An experimental survey of the gold concentrated from well water by a hydroelectrical extraction (HEE) method has been carried out in the famous Au metallogenic province in the eastern part of Shandong Province in northeastern China. This method is based on the existence of ionic Au in groundwater around ore bodies. Under the action of an external electric field, ionic Au migrates toward the cathode where the metal is absorbed by a special absorbent attached to the cathode. Promising areas for further exploration can be evaluated from the amount of Au in the absorbent.Distinct Au anomalies were discovered by the experimental survey. The results from the follow-up study of some of these anomalies have shown that most of them are related to the known placer Au deposits and to the primary Au occurrences. However, two new Au prospects and several new Au sources have been found which offer new targets for Au hunting in the area.The hydroelectrical extraction method is an effective technique of looking for Au in areas covered with transported overburden. Since the Au in well water can be concentrated on the cathode with the absorbent, the sensitivity requirement for Au analysis could be reduced. The method also provides a useful tool for the exploration of blind placer Au.