Nitrogen fixation driven by mesoscale eddies and the Kuroshio Current in the northern South China Sea and the East China Sea

N₂ fixation rates(NFR, in terms of N) in the northern South China Sea(nSCS) and the East China Sea(ECS) were measured using the acetylene reduction assay in summer and winter, 2009. NFR of the surface water ranged from 1.14 nmol/(L·d) to 10.40 nmol/(L·d)(average at(4.89±3.46) nmol/(L·d), n=11) in summer and 0.74 nmol/(L·d) to 29.45 nmol/(L·d)(average at(7.81±8.50) nmol/(L·d), n=15) in winter. Significant spatio-temporal heterogeneity emerged in our study: the anticyclonic eddies(AE)(P...     

广西红树林根际土壤放线菌的原位培养分离及其活性筛选

为了发掘广西红树林根际土壤的放线菌资源,本文利用原位培养装置,埋于根际土壤中俘获放线菌,30d后取回实验室,采用平板涂布法对4个地点的原位培养样品于15种培养基上进行分离纯化;对分离株基于16SrRNA基因序列进行系统发育分析;进一步进行抗菌活性和产酶活性检测。共分离得到113株放线菌。对其中33株放线菌进行测序,结果表明20株属于链霉菌属,11株属于拟诺卡氏菌属,1株属于伦兹氏菌属,1株与拟诺卡氏菌属相似性最高为90%,很可能属于放线菌一个新属。抗菌实验结果显示其中有7株、4株、18株、6株、10株、3株实验菌株分别对大肠埃希菌、铜绿假单胞菌、金黄色葡萄球菌、普通变形杆菌、乙型溶血性链球菌和肺炎克雷伯氏菌具有抑制作用;有55株、62株、24株、72株的实验菌株分别具有纤维素酶活性、淀粉酶活性、胶原蛋白酶活性、酯酶活性。原位培养可以丰富对广西红树林根际土壤的认识,分离到了新种甚至可能是新属的放线菌,分离得到的部分放线菌菌株具有较高生物活性,为后续工作提供了良好的实验材料。     

赤道西太平洋大气边界层的诊断分析

利用１９９３年ＥＮＳＯ事件爆发（４月）前酝酿时期“热带大洋与全球大气－海洋耦合响应试验”强化观测阶段“向阳红五号”科学考察船１５５°Ｅ，２°Ｓ定点海洋气象和高空大气探测资料，分析了赤道西太平洋大气边界层特征。结果表明：赤道西太平洋上空辐合对流区边界层内也有等温或逆温层存在，大气边界层物理参数变化与大尺度海－气变异有密切关系，赤道太平洋沃克环流加强、东移，边界层上部逆温层出现概率明显增大，边界层内高湿层湿度减小。另外，边界层内实测风速、风向廓线随高度的变化基本上不服从Ｅｋｍａｎ规律，但由其平均，分量合成得到的平均风随高度的变化却基本符合Ｅｋｍａｎ规律。     

Iron and phosphorus effects on the growth of Cryptomonas sp. (Cryptophyceae) and their availability in sediments from the Pearl River Estuary, China

Based on lab-culture experiments analyzing limitation and combination of iron and phosphorus on the growth of Cryptomonas sp. (Cryptophyceae), and the study of accumulation and release of Fe-bound P in sediment cores collected from the marine region of the Pearl River Estuary, China, reasons for the high frequency of phytoplankton bloom therein are discussed. Results show that the combined effect of Fe and P can obviously accelerate algal development, and the optimum culture conditions maintaining maximum growth rate are 0.05 μM Fe and 50 μM P. Cellular contents of Fe and P is consistent and the P:Fe molar ratio is 159:1. The optimum range of the P:Fe molar ratio in culture experiments for cell incubation is 500–1400. The vertical trends of total Fe and total P variations in sediments are parallel. Fe-bound P is the main species of inorganic sedimentary P. Through continuous leaching with agitation, 34–80% of exchangeable P and 4–23% of exchangeable Fe are concurrently released from the surficial sediments. This is a possible way by which nutrients are made available to phytoplankton. These factors might be responsible for a high frequency of harmful algal blooms in the Pearl River Estuary.     

Parametric vibration of submerged floating tunnel tether under random excitation

For the study of the parametric vibration response of submerged floating tunnel tether under random excitation,a nonlinear random parametric vibration equation of coupled tether and tube of submerged floating tunnel is set up.Subsequently,vibration response of tether in the tether-tube system is analyzed by Monte Carlo method.It may be concluded that when the tube is subjected to zero-mean Gaussian white noise random excitation,the displacement and velocity root mean square responses of tether reach the peak if the circular frequency of tube doubles that of tether;the displacement and velocity root mean square responses of tether increase as the random excitation root mean square increases;owing to the damping force of water,the displacement and velocity root mean square responses of tether decrease rapidly compared with tether in air;increasing the damping of the tether or tube reduces the displacement and velocity root mean square responses of tether;the large-amplitude vibration of tether may be avoided by locating dampers on the tether or tube.     

胶州湾多毛类名录及新纪录的描述

早在30年代,张玺教授就领导采集团对胶州湾的海产动物进行了系统的调査。全国解放后,中国科学院海洋研究所在胶州湾进行了大量的调查,吴宝铃教授发现了多毛类的许多新种和新记录。作者自1980年以来在胶州湾进行了连续三年的逐月底栖生物调査,又发现了许多过去未曾记录的多毛类,基本掌握了胶州湾多毛类的全貌。本文根据现有资料,报道胶州湾多毛类46科213种,其中前人已记录的有132种,作者补充了81种(名录中带*号者),有23种在我国为首次记录。本文对新记录的种类作简单描述,并在后列出胶州湾多毛类名录。     

2017年春季季风间期南海中北部浮游植物生长与微型浮游动物摄食

Phytoplankton growth rates and mortality rates were experimentally examined at 21 stations during the 2017 spring intermonsoon(April to early May) in the northern and central South China Sea(SCS) using the dilution technique, with emphasis on a comparison between the northern and central SCS areas which had different environmental factors. There had been higher temperature but lower nutrients and chlorophyll a concentrations in the central SCS than those in the northern SCS. The mean rates of phytoplankton growth(μ_0) and microzooplankton grazing(m) were(0.88±0.33) d~(–1) and(0.55±0.22) d~(–1) in the central SCS, and both higher than those in the northern SCS with the values of μ_0((0.81±0.16) d~(–1)) and m((0.30±0.09) d~(–1)), respectively.Phytoplankton growth and microzooplankton grazing rates were significantly coupled in both areas. The microzooplankton grazing impact(m/μ_0) on phytoplankton was also higher in the central SCS(0.63±0.12) than that in the northern SCS(0.37±0.06). The microzooplankton abundance was significantly correlated with temperature in the surface. Temperature might more effectively promote the microzooplankton grazing rate than phytoplankton growth rate, which might contribute to higher m and m/μ_0 in the central SCS. Compared with temperature, nutrients mainly affected the growth rate of phytoplankton. In the nutrient enrichment treatment,the phytoplankton growth rate(μn) was higher than μ_0 in the central SCS, suggesting phytoplankton growth in the central SCS was nutrient limited. The ratio of μ_0/μn was significantly correlated with nutrients concentrations in the both areas, indicating the limitation of nutrients was related to the concentrations of background nutrients in the study stations.     

3厘米扫描成像微波辐射计在海洋感感中的初次应用

所有物体都不断地发出电磁辐射,在微波谱段内黑体的辐射量B_bb可用瑞利—金斯公式表示:B_bb=2KT/λ2。     

中国近海盘管虫属(多毛纲：龙介虫科)的研究Ⅰ

中国近海多毛纲龙介虫科(石灰虫科)Serpulidae的分类区系,除吴宝铃和陈木(1985,1991)、陈木和吴宝铃(1978,1980)发表过6个新种以及杨德渐和孙瑞平(1988)作过一些常见种报道外,迄今尚未见系统研究报道。本文根据中国科学院海洋研究所历年调查、全国海洋综合调查和作者近年来调查补采的标本进行系统整理。共鉴定龙介虫科盘管虫属28种,其中11种为我国首次记录、1个新种。模式标本保存在中国科学院海洋研究所。     

α-1,4-葡聚糖裂解酶的动力学性质研究

红藻中的α-1,4-葡聚糖裂解酶是红藻淀粉的降解酶,除了 红藻淀粉外,它还可以作用于多种底物。文中分别以PNPG和可溶性淀粉作底物,以龙须菜的 几个品系作为材料,研究了该酶促反应的动力学方面的性质。以 PNPG作为底物,该酶在60m in内酶促反应速度与保温时间成线性关系;在野生型龙须菜品系中该酶的最适反应温度是50 ℃,在两种突变型品系中是40℃;最适pH范围在4.4～5.5;底物浓度是4mmol/L时达到最大反 应速度;葡萄糖对该酶促反应有明显的抑制作用。以可溶性淀粉作为底物,在反应60 min内 酶反应速度与保温时间成线性关系;最适反应温度为50℃;最适pH范围在5.0～5.8;底物浓 度是8mg/mL时达到最大反应速度;葡萄糖对可溶性淀粉的抑制作用比对PNPG的弱。