Occurrence of Two Distinct Types of Tissue Inhibitors of Metallo-proteinases-2 in Fugu rubripes

In this study, genes of two distinct tissue inhibitors of metalloproteinases-2 （TIMP-2） from Japanese puffer fish Fugu rubripes, Fugu TIMP-2a and TIMP-2b, were cloned. The open reading frames of Fugu TIMP-2a and TIMP-2b cDNAs are composed of 660 and 657 nucleotides and 220 and 219 amino acids, respectively. Both Fugu TIMP-2s contain 12 cysteine residues, which might form six disulfide bonds as in other animals＇ TIMP-2s. Reverse-transcribed polymerase chain reaction analysis showed the mRNAs of Fugu TIMP-2a and TIMP-2b to be expressed in some tissues examined with different expression patterns. These findings suggest that the two distinct Fugu TIMP-2s might perform different functions in Fugu tissues.     

Determination of the freshwater origin of Coastal Oyashio Water using humic-like fluorescence in dissolved organic matter

Coastal Oyashio Water (COW), defined as a water mass with a temperature lower than 2 °C and a salinity lower than 33.0, is distributed in the North Pacific Ocean off southeastern Hokkaido, Japan, from winter to spring. COW is rich in macronutrients and dissolved iron and is thus considered to affect the spring phytoplankton blooms in the Oyashio region. Although river water and sea-ice melt water have been considered freshwater end-members of COW, the contributions of these freshwater sources to COW have not been well described. In this study, the humic-like components in dissolved organic matter were first applied as a parameter to evaluate the freshwater end-members of COW in March 2015. Linear regressions with negative slopes were determined between the humic-like components and the salinity of COW. The intercepts of the regressions against the humic-like components were within the ranges of those observed for the local rivers of Hokkaido but were very different from those of sea ice. These findings suggest that river water contributed to the COW observed here as a freshwater end-member, although the contribution of sea-ice melt water to COW could not be evaluated. This novel approach also highlighted two different less-saline water masses in COW. The first was characterized by a lower temperature and relatively high levels of humic-like components, while the second was higher in temperature and had higher levels of humic-like components. It is suggested that these different characteristics are due to the contributions of water from different rivers and/or different effects of sea-ice melt water.     

Does suspended matter drained from the Isahaya freshwater reservoir cause organic enrichment in the northern Ariake Bay?

The inner part of Isahaya Bay was converted to a freshwater reservoir following the closure of the land claim dike in 1997. Turbid water drains into Isahaya Bay when water levels increase. We investigated whether particulate organic matter (POM) from the reservoir in Isahaya Bay has caused bottom organic enrichment in the northern part of Ariake Bay. Using potential end-members from before to after the rainy seasons, during which a frequent discharge from the reservoir was expected, stable isotope analyses were performed on sediments collected from Isahaya Bay and northern Ariake Bay. Each end-member was isotopically differentiated by δ¹³C and δ¹⁵N (riverine POM: ?28.5 to ?27.2‰ and 3.3–4.6‰; reservoir POM: ?25.7 to ?25.3‰ and 7.4–8.4‰; marine POM: ?21.8 to ?19.7‰ and 6.7–7.6‰; microphytobenthos estimated from consumers: ?16.1 to ?15.9‰ and 5.2–6.1‰, respectively). Sediment isotopic signatures fell within the mixing space defined by the signatures of the end-members. Marine POM contributed greatly to bottom sediments in both seasons in Isahaya Bay and Ariake Bay, ranging from ca. 60–70 and 40–60%, respectively. Reservoir POM contributed around 10% to bottom sediments. This percentage slightly increased in the sediment of Isahaya Bay after the rainy season, but decreased in the sediment of Ariake Bay. Thus, most of the POM discharged from the reservoir would not reach the northern part of Ariake Bay and would not be a major contributor to organic enrichment. This study is the first to quantitatively describe the contribution of drained reservoir POM outside Isahaya Bay.     

Environmental controls on phytoplankton production in coastal ecosystems: A case study from Tokyo Bay

Phytoplankton biomass and primary production were examined in their environmental context, for a semi-enclosed bay (Tokyo Bay, Japan) using data from monthly samples collected over a three-year period. Heavy precipitation and high surface temperatures in the late spring and summer gave rise to a highly-stratified water-column and stimulated a series of phytoplankton blooms, whereas during the winter, a weakly-stratified and deeply-mixed water-column led to a rapid decline in phytoplankton biomass under light-limited growth conditions. By incorporating pigment, photophysiological and optical data into a primary production model we show that daily, water-column primary production ranges from ∼160 mg C m⁻² d⁻¹ to 7600 mg C m⁻² d⁻¹. High water turbidity and deep vertical mixing, both separately and in concert, limit the light available for algal growth over much of the year. Annual primary production varied from 370 to 580 g C m⁻² y⁻¹. The relative influences of nutrient limitation and light limitation are assessed. A model is developed that describes this in an explicit manner using photophysiological parameters.     

Intensity measure conversion of fragility curves

In seismic risk assessment of structures, fragility functions are the probabilistic characterization of vulnerability at the component and/or structural level, expressing the probability of failure as a function of a ground motion intensity measure (IM). Fragility curves, in general, are structure- and site-specific, thus a comparison of fragility curves, then of vulnerability, is not straightforward across multiple structures. Also, it could be the case that hazard at a site of interest is not available for the IM originally considered in the fragility assessment. These situations require to convert fragility curves from an original IM to a target one. The present study addresses a hazard-consistent probabilistic framework for converting spectral acceleration-based IMs from an original IM to a target IM at a given site. In particular, three conversion cases, under different assumptions on the explanatory power of the involved IMs with respect to structural failure, are discussed: (a) a vector-valued IM consisting of the original and target IMs, magnitude, and source-to-site distance; (b) a vector-valued IM consisting of the original and target IMs; and (c) the original (scalar) IM only, assuming that structural response, given the IM, is statistically independent of the other ground motion variables. In this framework, the original fragility functions are characterized using the state-of-the-art methods in performance-based earthquake engineering, then the fragility curves as a function of the target IM are evaluated through applications of the probability calculus rules, ensuring consistency with the seismic hazard at the site of interest. The conversion strategy is illustrated through the applications to three-, six-, and nine-story Italian code-conforming reinforced concrete buildings designed for a high-hazard site in Italy. The study shows that, in most of the cases, the converted fragility curves have agreement with the reference curves directly developed in terms of the target IM. Cases in which least agreement was found are likely due to the models used to obtain the terms required by the conversion equations.     

Response of Equatorial Pacific Mean Temperature Field to Intraseasonal Wind Forcing

The effects of intra-seasonal wind forcing on the mean field of the tropical Pacific Ocean has been studied using an ocean general circulation model (GCM). Idealized intra-seasonal zonal wind forcing with zero mean, which propagates eastward, induces net eastward jets at the equator that shift the warm water pool to the east. The mean temperature of the upper 200 m of the ocean increases off the equator and decreases at the equator. The change is independent of the propagation speed of the intra-seasonal wind forcing. The magnitude of the change depends on the amplitude and the period of the forcing, and the ocean structure, while the spatial pattern is independent of these parameters. A simple shallow water model is used to explain these changes. It is found that the term responsible for the enhanced eastward Equatorial jet is the Reynolds stress term, which arises from a phase shift of the zonal current due to friction. The resultant convergence of eastward momentum on the equator and geostrophic adjustment of the interface to the change of zonal current brings about the thermal redistribution of the upper ocean seen in the GCM.     

Interannual Variability of Antarctic Hydrographic Structure and Frontal Zones along Meridional Sections between Syowa Station and Southern Africa

Six years of quasi-meridional crossings of the Antarctic Ocean from Antarctica to southern Africa between 4°W and 16°E longitude are analyzed, with emphasis on temperature and salinity distributions. Data are sparse, but (particularly in the southern part of the sections) adequate to show significant variations in the latitude/depth distributions and T-S characteristics of the principal water masses. In particular, the cross-sectional area occupied by Warm Deep Water (Weddell Deep Water) dropped by about one third in 1968. Positions of major features associated with the Antarctic Divergence varied by up to 5 degrees of latitude. The surface temperature gradient often observed at the Antarctic Divergence appears to be the surface expression of a shallow (possibly seasonal) thermocline which sometimes slopes upward to intersect the sea surface. There is a clear need for more closely spaced and consistently planned data from Antarctic cruises, particularly those on which physical oceanography is an ancillary activity. As a bare minimum, much more closely spaced surface temperature (and if possible also salinity) measurements would be useful.     

The Weathering of Granodiorite Porphyry in the Thiel Mountains, Inland Antarctica

The weathering of granodiorite porphyry is examined in the extremely cold and dry environment of the inland part of Antarctica. Weathering features include granular disintegration, rock varnish, and sheeting. Sheeting has gradually proceeded since the exposing of the nunatak, but the other types of weathering have not actively advanced in recent times.
Granular disintegration primarily comprises the release of individual crystals. The mechanism of this release is that cracks are created along crystal boundaries and cleavages in phenocrysts mainly due to differential thermal expansion. Plagioclase and hypersthene are released more readily than quartz. Disintegration of plagioclase has produced many prism-shaped holes, 0.5 to 2.0 mm in length, while fine-grained quartz crystals, 0.05 to 0.1 mm in diameter, are incidentally released without cracking.
The reddish brown (10R4/4) rock varnishes result from oxidation and consist of limonite, which fills cracks and penetrates into crystals. Ferrous iron in hypersthene, biotite, ilmenite and magnetite is transformed into limonite by oxidation. Manganese is not found in the varnishes. Sulfur, which is important for oxidation and which may have originated from adhered snow, is concentrated on the surface of the rock.
Sheeting has precipitated rock falls, and has gradually formed a gray mosaic on the varnished wall. The sheeting was caused by gravitational body force, which is internal stress of the rock body due to the mass of the overburden.     

A Late Holocene geomagnetic secular variation record from Erhai Lake, southwest China

A secular variation record of the geomagnetic field direction for the last 6.5  kyr has been obtained from the magnetization of sediment cores from Erhai Lake, southwest China. In order to make a comparison with this record, secular variation in east-central China was investigated by combining available magnetic field data from historical records and archaeomagnetic measurements since about 350 bc . The secular variation in Erhai Lake shows features consistent with the combined record, except for the oldest three observed declination swings in Sian from 720 to 900 ad . Many features of declination and inclination in China also occur in Japan. From 500 to 1000 ad , declination was westerly ranging from about −20° to −5° in Erhai Lake, east-central China, and Japan.