Pyrene induces a reduction in midbrain size and abnormal swimming behavior in early-hatched pufferfish larvae

Spills of heavy oil (HO) have an adverse effect on marine life. We have demonstrated previously that exposure to HO by fertilized eggs of the pufferfish (Takifugu rubripes) induces neural disruption and behavioral abnormality in early-hatched larvae. Here, two kinds of polycyclic aromatic hydrocarbons, pyrene and phenanthrene, were selected to examine their toxic effects on larval behavior of another pufferfish species (T. niphobles). Larvae exposed to pyrene or phenanthrene exhibited no abnormalities in morphology. However, those exposed to pyrene but not phenanthrene swam in an uncoordinated manner, although their swimming distance and speed were normal. The optic tectum, a part of the midbrain, of pyrene-exposed larvae did not grow to full size. Thus, these findings are indicated that pyrene might be a contributor to the behavioral and neuro-developmental toxicity, although there is no indication that it is the only compound participating in the toxicity of the heavy oil mixture.     

Effect of heavy oil on the development of the nervous system of floating and sinking teleost eggs

Heavy oil (HO) on the sea surface penetrates into fish eggs and prevents the normal morphogenesis. To identify the toxicological effects of HO in the context of the egg types, we performed exposure experiments using floating eggs and sinking eggs. In the course of development, HO-exposed embryos of floating eggs showed abnormal morphology, whereas early larva of the sinking eggs had almost normal morphology. However, the developing peripheral nervous system of sinking eggs showed abnormal projections. These findings suggest that HO exposed fishes have problems in the developing neurons, although they have no morphological malformations. Through these observations, we conclude that HO is strongly toxic to floating eggs in the morphogenesis, and also affect the neuron development in both floating and sinking eggs.     

Statistical analyses and modeling approaches to hydrodynamic characteristics in alluvial aquifer

This study is aimed at quantifying the difference in aquifer's response to recharge between some different locations in a fan aquifer and a delta aquifer for a preliminary study of revealing mechanisms of water transport in alluvial aquifer. The aquifer's response to recharge is statistically quantified with the two viewpoints: (1) timing and volume of recharge and (2) time length of aquifer's holding water. For the first point, a statistical model that links precipitation and groundwater level is introduced, and its parameters are identified using correlation analysis. Our results show that the recharge rate at the toe is higher than that at the apex and at the delta. For the second point, the concept of ‘memory effect’ of aquifer is adopted and quantified using the autocorrelation and spectral analyses. Our results show that the memory effect is longer at the toe of fan than at the apex, and thus, a temporary increase of water level has about five times as long‐term influence on subsequent water levels at the toe of the fan as at the apex. This study demonstrates that the statistical analyses and modeling of hydrological data are useful for characterizing aquifer's hydrodynamics. Copyright © 2012 John Wiley & Sons, Ltd.     

Tidal amplification and along‐strike process variability in a mixed‐energy paralic system prograding onto a low accommodation shelf,Edgeøya,Svalbard

The study describes the depositional development and sediment partitioning in a prograding paralic Triassic succession. The deposits are associated with the advance of large prism‐scale clinoforms across a shallower platform area. Approaching the platform, the limited accommodation and associated relative higher rates of deposition generated straighter clinoforms with lower foreset angles. The vertical restriction across the platform is interpreted to have amplified the tidal signature. Sediment was redistributed from the coast into increasingly sandy delta‐front deposits, compared to offshore equivalents. The deposits comprise extensive compound dune fields of amalgamated and increasingly clean sandbodies up‐section. Rapid deposition of significant amounts of sand led to differential subsidence and growth‐faulting in the delta front, with downthrown fault blocks further amplifying the tidal energy through funnelling. A mixed‐energy environment created along‐strike variability along the delta front with sedimentation governing process‐regime. Areas of lower sedimentation were reworked by wave and storm‐action, whereas high sedimentation rates preserved fluvially dominated mouth bars. A major transgression, however, favoured tidally dominated deposits also in these areas, attributed to increasing rugosity of the coastline. Formation of an extensive subaqueous platform between the coast and delta front dampened incoming wave energy, and tidally dominated deposits dominate the near‐shore successions. Meanwhile formation of wave‐built sand‐bars atop the platform attest to continued wave influence. The strong tidal regime led to the development of a heterolithic near‐shore tidally dominated channel system, and sandier fluvial channels up‐river. The highly meandering tidal channels incising the subaqueous platform form kilometre wide successions of inclined heterolithic stratification. The fluvially dominated channels which govern deposition on the delta plain are narrower and slightly less deep, straighter, generally symmetric and filled with cleaner sands. This study provides important insight into tidal amplification and sand redistribution during shallowing on a wide shelf, along with along‐strike process‐regime variability resulting from variations in sediment influx.     

Simultaneous volume measurements of post-perovskite and perovskite in MgSiO3 and their thermal equations of state

Simultaneous volume measurements of MgSiO₃ post-perovskite (PPv) and perovskite (Pv) were performed in a diamond anvil cell (DAC) combined with synchrotron X-rays. An externally-heated DAC was used in addition to a laser-heated DAC for the volume measurement experiment at high temperatures. The volume data were collected in the stability field of post-perovskite from 115 to 130 GPa. The temperature generated in the externally-heated and the laser-heated DACs for the volume measurement were up to 832 and 2330 K, respectively. Using two different but complementary heating techniques, we collected the data at a wide temperature range from 300 to 2330 K. The obtained P-V-T data for PPv and Pv were fitted to a third-ordered Birch-Murnaghan equation of state (EOS). For a precise comparison of the volume between the two phases, the EOSs were constructed based on the same pressure scale of MgO. The simultaneous volume measurements and the volumes calculated from the determined EOSs demonstrate that the volume difference between PPv and Pv of about 1.5% is almost constant with increasing temperature to 4000 K at the transition. At the base of the mantle, this density difference corresponds to a temperature anomaly of 1300 K without the phase transition due to the very small thermal expansivity of minerals, which has a significant effect on mantle dynamics. The thermal expansivity contrast between the top and the bottom of the mantle is a factor of 3.6. From a mantle convection study, this value suggests that huge and hot plumes are formed at the core–mantle boundary.     

Toxicogenomic analysis of immune system-related genes in Japanese flounder (Paralichthys olivaceus) exposed to heavy oil

Heavy oil contamination is one of the most important environmental issues. Toxicities of polycyclic aromatic hydrocarbons (PAHs), including immune toxicities, are well characterized, however, the immune toxic effects of heavy oil, as a complex mixture of PAHs, have not been investigated. In the present study, we selected Japanese flounder (Paralichthys olivaceus) as a model organism, and observed alteration of immune function by the exposure to heavy oil. To analyze the expression profiles of immune system-related genes, we selected 309 cDNAs from our flounder EST library, and spotted them on a glass slide. Using this cDNA array, alteration of gene expression profiles was analyzed in the kidneys of flounders exposed to heavy oil. Six Japanese flounders (mean body weight: 197 g) were acclimated to laboratory conditions at 19–20 °C. Three fish were exposed to heavy oil C (bunker C) at a concentration of 3.8 g/L for 3 days, and the others were kept in seawater without heavy oil and used as the control. After the exposure period, the fish were transferred into control seawater and maintained for 4 days, and then they were dissected and their kidneys were removed. Total RNA was extracted from the kidney samples to use in gene expression analyses. The microarray detected alteration of immune system-related genes in the kidneys of heavy oil-exposed flounders, including down-regulation of immunoglobulin light chain, CD45, major histocompatibility complex class II antigens and macrophage colony-stimulating factor precursor, and up-regulation of interleukin-8 and lysozyme. These results suggest that pathogen resistance may be weakened in heavy oil-exposed fish, causing a subsequent bacterial infection, and then proinflammatory genes may be induced as a defensive response against the infection. Additionally, we found candidate genes for use as biomarkers of heavy oil exposure, such as N-myc downstream regulated gene 1 and heat shock cognate 71 kDa proteins.     

Elasticity of MgO to 130 GPa: Implications for lower mantle mineralogy

The aggregate shear wave velocities of MgO (periclase) have been determined throughout Earth's lower mantle pressure regime approaching 130 GPa using Brillouin spectroscopy in conjunction with synchrotron X-ray diffraction technique in a diamond anvil cell apparatus. We found that the extrapolations of the high-pressure shear wave velocities and shear moduli to ambient pressure are highly consistent with earlier studies. However, the measurements over a wide pressure range revealed that the pressure derivative of the shear modulus (dG/dP = G₀′) of MgO is 1.92(2), which is distinctly lower than that of previous lower-pressure experiments. Compared with the previous results on (Mg,Fe)O ferropericlase, there is no clear correlation between iron content and G₀′. We calculate that the shear wave velocity profile of lower mantle along the adiabatic geotherm applied by the lower G₀′ value of periclase can remarkably well reproduce the global seismological 1-D velocity profile model with uniform composition model. The best-fitting result indicates the possibility of a lower mantle mineralogy with ~ 92 vol.% silicate perovskite phase, implying that the bulk composition of lower mantle is likely not to be pyrolitic but more chondritic. The present acoustic measurements performed over the large pressure range have thus led us to a better understanding of compositional model of the Earth's lower mantle.