Effects of 17beta-estradiol on the reproduction of Java-medaka (Oryzias javanicus), a new test fish species

17β-estradiol (E2) is a female hormone which is known to be one of the strongest estrogenic chemicals in the environment. The present study investigated the effects of E2 on the reproduction of the estuarine fish, Java-medaka (Oryzias javanicus). Starting from the embryonic stages, Java-medaka was exposed for 6 months to 9.5, 16, 68, 159 and 243 ng/l of E2. The fecundity of Java-medaka exposed to E2 levels >16 ng/l was significantly lower than that of the control. The appearance of secondary sexual characteristics seemed to be inhibited by exposure to 159 and 243 ng/l E2. Vitellogenin concentrations in the liver of male fish exposed to 68, 159 and 243 ng/l were significantly higher than that of the control. Thirty-three and sixty percent of male fishes exposed to 159 and 243 ng/l, respectively, had testis-ova. These results suggest that reproduction in the Java-medaka, especially the male fish, could be affected by exposure to E2 concentrations greater than 16 ng/l.     

Atmospheric wave propagations in the mesopause region observed by the OH(8,3) band,NaD, O2A(8645Å) band and OI 5577 Å nightglow emissions

Simultaneous measurements of the upper mesospheric NaD and OH(8,3) band emissions by meridional scanning photometers, and the OI 5577 Å, O₂ Atmospheric band at 8645 Å, NaD and OH(8,3) band emissions by multi-channel tilting filter type zenith photometers have been carried out at Cachoeira Paulista (22.7°S, 45.0°W), Brazil. On two nights during the period May–August 1983, the meridional scanning observations showed horizontal intensity gradients and phase propagations. The nocturnal intensity variations on one of these occasions 13–14 June 1983, which was a magnetically disturbed night with 4 ?k_p? 8, also showed vertical phase propagation. In this paper, we present these observations and discuss the possible effects of the horizontal wind system and of gravity wave propagation.     

Shaking table tests on the lateral response of a pile buried in liquefied sand

This paper presents an experimental study on the lateral resistance of a pile subjected to liquefaction-induced lateral flow. To observe the soil surrounding the pile during liquefaction, it was modeled as a buried cylinder that corresponded to a sectional model of the prototype pile at a certain depth in the subsoil. In order to create a realistic stress condition in the model ground, the model was prepared in a sealed container and the overburden pressure was applied to the ground surface by a rubber pressure bag. The model pile was actuated back and forth through rods attached on each side by an electro-hydraulic actuator.This paper focuses on observing the deformation of the liquefied soil surrounding the pile when a large relative displacement between the pile and the soil is induced. The loading rate effect on the lateral resistance of the pile in the liquefied sand and the influence of the relative density are also investigated.Test results show that a larger resistance is mobilized as the loading rate becomes higher. When the loading rate is higher, the cylinder displacement required for the lateral resistance becomes smaller. It has been also observed that as the relative density of the soil increases, dilatancy of the soil in front of the pile also increases.     

Cenomanian (Upper Cretaceous) carbon isotope stratigraphy of terrestrial organic matter for the Yezo Group, Hokkaido, Japan

Abstract Carbon isotope fluctuations of sedimentary organic matter along the two geological traverses in the Yezo Group, Hokkaido, northern Japan, elucidate a detailed chemostratigraphy for the Cenomanian Stage on the northwestern Pacific margin. Visual characterization of the kerogen from mudstone samples shows that the major constituents of sedimentary organic matter originated as terrestrial higher plants. The atomic hydrogen/carbon ratios of the kerogen suggest that the original δ¹³C values of terrestrial organic matter (TOM) have not been affected significantly by thermal diagenesis. The patterns in two δ¹³C_TOM curves are similar and independent of changes in lithology and total organic carbon contents, which suggests that TOM was mixed sufficiently before the deposition in the Yezo forearc basin for the δ¹³C composition having been homogenized. In addition, this implies that the Hokkaido δ¹³C_TOM profiles represent the averaged temporal δ¹³C variations of terrestrial higher‐plant vegetation in the hinterlands of northeast Asia during Cenomanian time. Three shorter‐term (ca. 0.1 my duration) positive‐and‐negative δ¹³C_TOM fluctuations of ∼1‰ are present in the Lower to Middle Cenomanian interval in the Yezo Group. On the basis of the age‐diagnostic taxa (ammonoids, inoceramids and planktic foraminifers), these discrete δ¹³C_TOM events are interpreted to be correlated with those in the δ¹³C curves of pelagic carbonates from European basins. The correlation of δ¹³C events between the European and Yezo Group sections suggests that the shorter‐term δ¹³C fluctuations in Cenomanian ocean‐atmosphere carbon reservoirs are useful for global chemostratigraphic correlation of marine strata. In particular, the correlation of δ¹³C fluctuations of the so‐called ‘Mid‐Cenomanian event’ (MCE) implies: (i) the δ¹³C variations of global carbon reservoir during the MCE are precisely recorded in the δ¹³C_TOM records; and (ii) the MCE δ¹³C_TOM event is an efficient chronostratigraphic index for the Lower/Middle Cenomanian boundary of the Mid‐Cretaceous sequences.     

An atomic level study of rhenium and radiogenic osmium in molybdenite

Local atomic structures of Re and radiogenic Os in molybdenite from the Onganja mine, Namibia, were examined using X-ray absorption fine structure (XAFS). Rhenium L_III-edge X-ray absorption near-edge structure (XANES) and extended X-ray absorption fine structure (EXAFS) show that the oxidation state of Re, the interatomic distances between Re and the neighboring atoms, and the coordination number of Re to S are very similar to those of Mo in molybdenite. The results confirm that Re is present as Re(IV) in the Mo site in molybdenite.Measurement of L_III-edge XANES and EXAFS of a minor concentration (8.55 ppm) of radiogenic Os was accomplished in fluorescence mode by removing the interfering X-rays from Re and other elements using a crystal analyzer system. The data indicate that the oxidation state of radiogenic Os is Os(III) and Os(IV) and clearly different from Os(II) in natural sulfide minerals, such as OsS₂ (erlichmanite). XANES data also suggest that radiogenic Os does not form a secondary Os phase, such as OsS₂ or Os metal, in molybdenite.EXAFS of radiogenic Os was successfully simulated assuming that Os is present in the Mo site in molybdenite. The data are consistent with the XANES data; Os does not form Os phases in molybdenite. The EXAFS simulation showed that the interatomic distance between Os and S is 2.27 Å, which is 0.12 Å smaller than the distances of Re-S and Mo-S (2.39 Å) in molybdenite. Similar valences and ionic sizes of Re and Mo in molybdenite support the fact that large amounts of Re can be incorporated into the Mo site as has been observed in previous studies, whereas the different properties of Os compared to Mo and Re suggested here support much lower abundance of common Os in molybdenite. This makes molybdenite an ideal mineral for the Re-Os geochronometer as shown in many studies. However, the shorter distance between radiogenic Os and S compared to those of Re-S and Mo-S in molybdenite suggests that the radiogenic Os has a smaller ionic size than Re(IV) and Mo(IV). Furthermore, Os may be partly present as Os(III). Smaller and lower charge Os can diffuse faster than larger and higher charge Re in molybdenite at a given set of conditions. Hence, our study provides an atomic-level explanation for the high mobility of Os compared to Re, which has been suggested by earlier workers using laser ablation ICP-MS.     

Occurrence of arsenic (V) in forearc mantle serpentinites based on X-ray absorption spectroscopy study

We examined As concentration, mineralogical site, and oxidation state in the serpentinites associated with the Tso Morari eclogitic rocks in the Indus suture zone, northwest Himalaya, to examine how highly fluid-soluble elements like As are transferred from slabs to arc magmas in subduction zones. The serpentinite samples represent hydrated peridotites at the base of the mantle wedge beneath Eurasia, and were exhumed from a depth of ∼100 km during subduction of the Indian continental margin. The bulk serpentinite samples contain total As concentrations ranging from 6 to 275 ppm. Arsenic in the samples is most likely present with magnetite and antigorite, since electron probe analysis yielded up to 90 ppm As in these minerals. X-ray absorption near-edge structure spectra indicate that As in serpentinites is mostly As(V) and that the neighboring atoms of As are O, although there are minute grains of sulfides and arsenides. The ratio of As(V) to total As is greater in samples with higher As contents, suggesting that the major source of As was oxidized As(V), introduced to the mantle wedge. Arsenic(V), originally adsorbed on Fe-oxides in slabs and overlying sediments, was most likely liberated during their subduction and incorporated subsequently in the overlying mantle wedge. Our data constrain the introduction of As into the mantle wedge at relatively shallow levels, much shallower than 25 km. Arsenic incorporated in the serpentinites was transported to deeper levels by mantle flow downward along the subduction zone, to be subsequently exhumed together with eclogitic rocks.     

Effects of 17β-estradiol on the reproduction of Java-medaka (Oryzias javanicus), a new test fish species

17β-estradiol (E2) is a female hormone which is known to be one of the strongest estrogenic chemicals in the environment. The present study investigated the effects of E2 on the reproduction of the estuarine fish, Java-medaka (Oryzias javanicus). Starting from the embryonic stages, Java-medaka was exposed for 6 months to 9.5, 16, 68, 159 and 243 ng/l of E2. The fecundity of Java-medaka exposed to E2 levels >16 ng/l was significantly lower than that of the control. The appearance of secondary sexual characteristics seemed to be inhibited by exposure to 159 and 243 ng/l E2. Vitellogenin concentrations in the liver of male fish exposed to 68, 159 and 243 ng/l were significantly higher than that of the control. Thirty-three and sixty percent of male fishes exposed to 159 and 243 ng/l, respectively, had testis-ova. These results suggest that reproduction in the Java-medaka, especially the male fish, could be affected by exposure to E2 concentrations greater than 16 ng/l.     

Spectrum of slowly varying component of solar radio emission on millimeter wavelengths

The spectrum of the S-component of solar radio emission has been investigated at 4 GHz, 17 GHz, 35 GHz, 70 GHz, and 94 GHz. The spectrum for a spot group which appeared late in March 1966 (McMath plage No. 8223), seems to be flat at the frequencies above about 35 GHz. This implies that the emission is due to pure free-free emission at the frequencies above 35 GHz.     

Water status in winter wheat grown under salt stress

Properties of the soil surface layer, the temporal pattern of the microclimate variables as well as crop condition were combined to analyze the characteristics of the evapotranspiration from winter wheat fields in a saline soil area. In order to accomplish this analysis, evapotranspiration was divided into evaporation from the soil and transpiration from wheat. Moreover, the effect of soil salinity on evapotranspiration was evaluated through the relationship between actual evapotranspiration and potential evapotranspiration (Ea/Eo) and the total soil water potential (y) was divided into two components: matric potential (yM) and osmotic potential (yo). Two sites with different salinity levels were chosen for this study, located in Hebei Province, China. Measurements were conducted in April-May 1997 and May 1998. The Bowen ratio method was used to estimate the actual evapotranspiration (Ea), whereas potential evapotranspiration (Eo) was estimated using Penman’s equation. Measurements of soil evaporation (Es) were obtained with micro-lysimeters, and transpiration was calculated from the difference between Ea and Es. The results show that transpiration comprised on average almost 80 % of total evapotranspiration. Evaporation from the soil differed slightly between years, but this variation was dominated by the leaf area index (LAI), which ranged from 4 to 5 during the study period of 1997 and 1998. Soil electric conductivity (EC), which is directly related to osmotic potential, ranged from 1.9 to 3.5 mS cm-1 in 1997 and was negligible in 1998. Our results indicate that lower osmotic potential decreases the total soil water potential, thus affecting plant transpiration. Hence, it is possible to say that soil salinity actually decreases evapotranspiration from winter wheat fields.